Benzo(a)pyrene 독성에 의한 사람 림프아 세포(NC-37)에서 c-myc 유전자의 발현

하봉준,정인철,곽충근,조무연 고신대학교 의학부 1996 高神大學校 醫學部 論文集 Vol.11 No.1-2

To investigate expression in c-myc gene by a chemical carcinogen benzo(a)pyrene(BP), human lymphoblast NC-37 cells were exposed to various concentrations of BP and the c-myc gene expression was evaluated by northern and slot blot hybridization methods. When NC-37 cells exposed to BP of 0-4㎍/ml were treated with HindⅡ/XbaⅠ restriction enzyme, the c-myc genes were cut at the same region regardless of BP concentration. The site of cleavage by the restriction enzyme, HindⅡ/XbaⅠ was identical in the control, BP-treated and BP-washed cells. However, the m-RNA expression in slot blot hybridization appeared to be 4-5 times higher in BP-treated cells than in the control, and this effect was partially removed by washing the BP. When the DNA isolated from NC-37 cells exposed to various concentrations(0, 2, 4㎍/ml) of BP were amplified by polymerase chain reaction using a primer containing c-myc exon Ⅰ, the resulting DNA were of the same size in all groups. These results suggest that overexpression of c-myc oncogene may be required for the malignant transformation and maintenance in benzo(a)pyrene poisoned human lymphoblast NC-37 cells.

유전자 재조합 사람 IFN-beta인 제네베타와 베네세린의 생물학적 활성의 비교

하종천,이협준,박봉환,김진숙,유대근,남상윤 전주대학교 자연과학종합연구소 2003 전주대학교 자연과학연구소 학술논문집 Vol.15 No.1

Background: Recombinant IFN-beta products have been approved for the treatment of several diseases. Recently, Inbionet, Co. has successfully developed rhIFN-beta product, Genebeta. This study was performed in a purpose of confirming the biological activity of Genebeta by comparison with another recombinant IFN-beta, Beneserin which was already approved. Methods: In vitro antiporliferative activity, regulation of MHC class I expression, antiviral activity, regulation of natural killer cell cytotoxicity were studied for functional comparison of these two rhIFN-beta products. Results: Proliferation assay data showed that two recombinant IFN-beta inhibited T cell and Daudi cell proliferation to a similar extent. When antiviral activity was compared, there was no significant difference between them. The treatment of these two rhIFN-beta also resulted in a similar level of upregulation of class I expression on the surface of A549 cells. Cytotoxicity experiment against tumor cells revealed that both of rhIFN-beta could enhance NK cell cytotoxicity to comparable level. Conclusion: All comparison data indicated that rhIFN-beta products, Genebeta and Beneserin have comparable potentials as a biological response modifier.

매크로/마이크로 레벨 볼트 결합 구조물에서의 실험적/해석적 방법을 통한 시스템 특성 비교

김봉석,이성민,이문구,이수훈,Jun Ni,송준엽,이창우,하태호 한국공작기계학회 2006 한국공작기계학회 추계학술대회논문집 Vol.2006 No.-

As the micro technology and miniaturization of a structure and component in the last couple of decades have been concretely realized, the understanding of structural and dynamic characteristics and prediction of dynamic behavior are indispensable to the miniaturized structures and micro machines. This paper shows the effect of dynamic characteristics in bolt-jointed structures by comparing natural frequencies and mode shapes between macro and micro-scale beams through experimental modal analysis and finite element analysis with a1 8 test models by materials, by size, and by joining condition; that is, SS41 and A16061-T651, micro and macro, and monolithic beam and bolt-jointed beam.

영동지역 주요 3개 하천의 수질실태 및 관리에 관한 연구

박광하,민선홍,최봉종,전방욱 강릉대학교동해안지역연구소 1994 東海岸硏究 Vol.5 No.1

영동지방의 주요 3개 하천의 수질을 조사하고 QUAL-2E 모델을 적용하여 보았다. 강릉남대천은 각 수질항목에서 시기별, 정점별로 큰 차이를 보였으며, 일반적으로 상류의 수질은 하류로 갈수록 생활오수의 유입으로 수질이 악화되는 전형적인 양상을 나타내었다. 양양남대천은 강릉남대천 및 삼척오십천에 비하여 시기별 변이폭이 좁았으며 각 정점별의 수질도 양호한 것으로 나타났다. 삼척오십천은 시기별보다는 정점별 수질차이가 크게 나타났으며 오염된 상류의 수질이 자정작용에 의하여 개선되다가 다시 생활오수의 유입으로 인하여 악화되는 특이한 양상을 보였다. 모델링 결과 하천의 유속, 유량등의 실측 자료가 부족함에도 불구하고 강릉남대천과 양양남대천은 실측치와 예상치가 높은 상관관계를 나타내었다. 또한 이 모델을 사용하여 강릉남대천의 수질예측을 시도하였다. The water quality was monitored and QUAL-2E modelling was applied in three main stream of Youngdong area. Kangnung Namdaecheon was shown to be a typical stream that the downstream water quality was worse than in the upperstream. Water quality of Yangyang Namdaecheon was better than that of Kangnung Namdaecheon and Samchuk Osipcheon. The spatial variation of water quality was greater than the temporal variation in Samchuk Osipcheon: the polluted upperstream water got better in the midstream, but got worse gain in the downstream. Despite of the lack of suitable measurement data, Kangnung Namdaecheon and Ynagyang Namdaecheon showed good relationship between measured and obtained data: however Samchuk Osipcheon, which had many point sources and much pollution load, showed poor relationship. Based on this model, future water quality of Kangnung Namdaecheon was prospected.

가상공간의 가공 공정과 상태 구현에 관한 연구

이수훈,김봉석,홍민성,김종민,JUN NI,박상호,송준엽,이창우,하태호 한국공작기계학회 2005 한국공작기계학회 춘계학술대회논문집 Vol.2005 No.-

This paper presents virtual machining system in order to realize turning process in virtual space. A reliable virtual turning process simulation was developed based on the surface shaping system which is capable of considering geometric model, thermal error model, and vibration model. Accuracy of surface shape resulting from proposed machining simulator was verified experimentally. This paper also developed the watchdog agent that continuously assessed, diagnosed, and predicted performance of products and machines in machining. The Watchdog agent extracted feature signal using time-frequency analysis among various signals from multi-sensor and evaluated machining condition using performance confidence value.

강구조 인장 접합부의 블록전단 파단

이은택,김만수,전봉진,이향하 중앙대학교 건설산업기술연구소 2003 건설산업기술연구소 논문집 Vol.4 No.-

The junction of tension materials, connected with high-strength bolt, should be designed to bear net section destruction, fastener fracture, and fracture of block shear considering yielding of the shear plane and the impact of shear retardation. Block shear among them means that tension side vertical and shear plane parallel to and shear plane horizontal in the direction of weight is torn in the form of "square piece;" it is a type of fracture well known in the junction such as simple beam or gasset plate connected with the bolts. In other words, block shear is a situation of limitations where member resistance is determined by shear strength generated by the destruct line (DL) and tension strength generated by right-angled sections. Since the design law of limitation status of steel architecture was established in 1997, investigation into detailed block shear calculation formula is not thoroughgoing enough, and it is not put into practice very well. This study showed that fracture shape and the proof stress of the fracture of 'ㄱ' steel (angle) are emphasized based on the previous results in order to find out feasibility of design criteria of nlock shear in terms of design law of limitation status and verify calculation formula of block shear.

자율신경계의 활성도 측정을 위한 Data Acquisition System의 개발 및 임상응용

신동구,박종선,김영조,심봉섭,이상학,이준하 영남대학교 기초/임상의학연구소 2001 Yeungnam University Journal of Medicine Vol.18 No.1

Background: Power spectrum analysis method is a powerful noninvasive tool for quantifying autonomic nervous system activity. In this paper, we developed a data acquistion system for estimating the activity of the autonomic nervous system by the analysis of heart rate and respiratory rate variability using power spectrum analysis. Materials and methods: For the detection of QRS peak and measurement of respiratory rate from patient's ECG, we used low-pass filter and impedance method respectively. This system adopt an isolated power for patient's safety. In this system, two output signals can be obtained: R-R interval(heart rate) and respiration rate time series. Experimental ranges are 30∼240 BPM for ECG and 15∼80 BPM for respiration. Regults: The system can acquire two signals accurately both in the experimental test using simulator and in real clinical setting. Conclusion: The system developed in this paper is efficient for the acquisition of heart rate and respiration signals. This system will play a role in research area for improving our understanding of the pathophysiologic involvement of the autonomic nervous system in various disease states.

정신분열병에 대한 리스페리돈의 효과 및 안정성

이민수,김용구,김영훈,연병길,오병훈,윤도준,윤진상,이철,정희연,강병조,김광수,김동언,김명정,김상훈,김희철,나철,노승호,민경준,박기창,박두병,백기청,백인호,손봉기,손진욱,양병환,양창국,우행원,이정호,이종범,이홍식,임기영,전태연,정영조,정영철,정인과,정인원,지익성,채정호,한상익,한선호,한진희,서광윤 大韓神經精神醫學會 1998 신경정신의학 Vol.37 No.1

연구목적 : 본 시험의 목적은 임상시험 시작전에 연구자들을 대상으로 PANSS Workshop을 통하여 PANSS, ESRS에 대한 국내에서의 표준화 작업을 구축하고 새로운 정신병 치료제인 리스페리돈의 효과와 안정성을 재확인하여 리스페리돈 사용에 대한 적정화를 이루는데 있다. 연구방법 : 1996년 4월부터 1996년 9월까지 국내 39개 대학병원 정신과에 입원중인 혹은 증상이 악화되어 입원하는 정신분열병 환자 377명을 대상으로 다시설 개방 연구를 시행하였다. 1주일간의 약물 배설기간을 가진후, 리스페리돈을 8주간 투여하였고, 기준점, 1주, 2주, 4주, 그리고 8주후에 평가되었다. 용량은 제1일에는 리스페리돈 1mg씩 1일 2회, 제2일에는 2mg씩 1일 2회, 제3∼7일에는 3mg씩 1일 2회 투여하였다. 이후 환자의 임상상태에 따라 임의로 증량할 수 있으며, 최대 일일 16mg을 초과하지 않도록 하였다. 추체외로 증상을 조절하기 위한 투약을 허용하였다. 임상증상 및 부작용의 평가는 PANSS(Positive and Negative Syndrome Scale), CGI(Clinical Global Impression) 그리고 ESRS(Extrapyramidal Symptom Rating Scale)을 사용하였다. 연구결과 : 377명중 343명(91%)이 8주간의 연구를 완결하였다. 치료 종결시점인 8주후 PANSS 총점수가 20% 이상 호전된 경우를 약물 반응군으로 정의할때, 약물반응군은 81.3%였다. 리스페리돈에 반응하는 예측인자로는 발병연령, 이전의 입원 횟수, 유병기간이 관련 있었다. 리스페리돈은 1주후부터 PANSS양성, 음성, 및 일반정신병리 점수상에 유의한 호전을 보여 효과가 빨랐다. CGI의 경우도 기준점에 비해 1주후부터 유의한 감소를 나타내었다. ESRS의 경우, 파킨슨 평가점수는 기준점과 비교해 투여 1주, 2주, 4주후 유의하게 증가되었다가 8주후 기준점과 차이가 없었다. Dystonia 평가점수는 1주후만 유의한 증가를 보였으며, dyskinesia 평가점수는 유의한 차이가 없었다. 혈압, 맥박수의 생명징후 및 일반 혈액학 검사, 생화학적 검사, 심전도 검사에서 유의한 변화는 없었다. 결 론 : 이상의 다시설 개방 임상 연구를 통해 리스페리돈은 정신분열병 환자에서 양성증상뿐만 아니라 음성증상 및 전반적인 증상에도 효과적인 것으로 사료된다. 보다 명확한 평가를 위해서는 다른 항정신병약물과의 이중맹검 연구가 필요할 것으로 생각되며, 또한 장기적 치료에 대한 평가도 함께 이루어져야 하겠다. Objective : The purpose of this study was to investigate the efficacy and safety of risperidone in the treatment of Korean schizophrenic patients. Method : This multicenter open study included 377 schizophrenic patients drawn from 39 university hospitals. After a wash-out period of 1 week, the schizophrenic patients were treated with risperidone for 8 weeks and evaluated at 5 points ; at baseline, and 1, 2, 4 and 8 weeks of treatment. The dose was increased from 2mg/day(1mg twice daily) to 6mg/day(3mg twice daily) during the first week and adjusted to a maximum of 16mg/day over the next 7 weeks according to the patient's clinical response. Medication to control extrapyramidal symptoms was permitted. The psychiatric and neurological status of the patients was assessed by PANSS, CGI, and ESRS scales. Results : 343(91%) of 377 patients completed the 8-week trial period. Clinical improvement, as defined by a 20% or more reduction in total PANSS score at end point, was shown by 81.3% of patients. The predictors of response to risperidone were associated older age, shorter duration of illness, fewer previous hospitalization. Risperidone had rapid onset of action ; a significant decrease of the total PANSS and three PANSS factor(positive, negative, general), and CGI was already noticed at the end of first week. For the ESRS, parkinsonism rating scores were significantly increased until week 4 comparing with baseline. Dystonia rating scores were significantly increased until week 1, and dyskinesia rating scores were not significantly changed during the study. Laboratory parameters including vital sign, EKG, hematological, and biochemical values showed no significant changes during the trial. Conclusions : This study suggests that risperidone is generally safe and effective against both the positive and negative symptoms in our group of patients.

Shading Level Affects Growth and Fruit Set of Paprika (Capsicum Annuum L.) ‘Colletti’

Jun Bong Ha,Chul Geon An,Chae Shin Lim,Eun Hwa Jung,Chang Hwan Sohn,Ou Soo Kim,Byoung Ryong Jeong 한국원예학회 2011 한국원예학회 학술발표요지 Vol.2011 No.5

New Faunistic Data of Tortricinae (Lepidoptera, Tortricidae) for the North Korean Fauna

Bong-Kyu Byun,Bong-Woo Lee,Kwan-Ho Bae,Jun-Keun Choi,Ha Yong Choi 한국응용곤충학회 2012 한국응용곤충학회 학술대회논문집 Vol.2012 No.10

The first record of the subfamily Tortricinae from North Korea was reported by Zhu (1969), comprising 17 species. Later Park and Byun (1991) added 11 species of the subfamily. And Park and Razowski (1991) added 16 species of the tribe Tortricini. Jaros et al. (1992) reported 11 species of Tortricinae from North Korea, based on the material Czech Academy of Sciences, Prague, Czech Republic(CAS). Byun et al. (1998) enumerated 350 species of Korean Tortricidae, citing 63 species of Tortricinae and 40 species of Olethreutinae from North Korea. Later Razowski (1999) provided a list of Korean Tortricidae with 176 of North Korea. Despite these studies, much of the North Korean Tortricidae deposited in the HNHM remained unstudied. Recently the first author examined the totricid material in the HNHM and reported 7 newly recorded species from North Korea (Byun et al., 2007). In the present study, we provide the faunistic information for ten species of Tortricinae, including their localities. This study was carried out under the support of Korea National Arboretum and Korea Forestry Research Institute.