Hydrocortisone에 依하여 Inducek된 Serine Dehydratase의 代謝?果

趙武衍,金濟炫 中央醫學社 1975 中央醫學 Vol.29 No.2

Hepatic serine dehydratase was induced with 5 mg of hydrocortisone by intraper-itoneal administration in albino rats weighing around 200 gm. Serine metabolism was observed in intact and in the isolated perfused liver. The results were as follows: 1) Serine dehydratase activity was two times higher in the hydocortisone treated group than in the controls in intact and in the isolated perfuse liver. 2) In vivo experiments blood concentrations of pyruvate was lower in the hydrocortisone group than in the control, whereas respiratory14CO2 was no dif?ference between hydrocortisone and control groups. 3) In the isolated perfuse liver: i) net cumulative production of pyruvate in the hydrocortisone group was less than that of the control in group after 200mg and 1. 2gm administrations of L-serine to each. group. ii) net cumulative production of glucose after 200mg administration was lower in the hydrocortisone group than that of the control. This lower value was maintained until the end of the perfusion. After administration of 1. 2gm serine glucose production was continuously raised but no difference between the hydrocortisone and control groups was observed. iii) liver glycogen content in the hydrocortisone group was higher than that of the control but there is no difference after administration of different dosages of L-serine. From the above results it may be concluded that induced serine dehydratase activity was not parallel to the production of pyruvate, glucose, respiratory CO2 and liver glycogen.

Serine Dehydratase 의 Hormonal Induction 의 생리적 의의에 대한 실험적 연구

조무연,김제현 생화학분자생물학회 1981 BMB Reports Vol.8 No.1

백서에서 L - Tryptophan 의 과량 투여시 간장내 Triglyceride 축적에 미치는 호르몬의 효과

조무연,김윤수 ( Moo Youn Cho,Yoon Soo Kim ) 생화학분자생물학회 1978 BMB Reports Vol.11 No.1

When an excess amount of L-tryptophan (0.34mmole) was administered intraperitioneally to a normal rat the triglyceride content in the liver increased two times that of the normal control in three hours, but no significant changes of triglyceride content in the liver of adrenalectomized rat was noted, however, the triglyceride content in liver and FFA in the plasma of adrenalectomized rat administered epinephrine and L-tryptophan together was increased as much as one and a half times than that of the control adrenalectomized rat which received L-tryptophan or epinephrine only. No effects of hydrocortisone, glucagon and insulin on the triglyceride accumulation in the liver were noted in adrenalectomized rat receiving each hormone or L-tryptophan only, or hormone and L-tryptophan together. Therefore, it was confirmed that the triglyceride accumulation in the liver induced by L-tryptophan is due to the indirect effect of L-tryptophan through stimulation of epinephrine secretion.

식이조성을 달리한 백서에 만성 알콜투여가 간장조직 내 malic enzyme 및 ATP-citrate lyase활성에 미치는 실험적 연구

조무연,김윤수,Cho, Moo-Youn,Kim, Yoon-Soo 생화학분자생물학회 1978 한국생화학회지 Vol.11 No.1

간장조직 내 lipogenic enzyme인 malic enzyme과 ATP-citrate lyase의 활성이 식이조성을 달리한 사료로 사육한 백서에서 어떻게 영향을 받으며 이동안 만성알콜투여 (6.7Cal/day)로 오는 효소활성 및 간장조직 내 지방 형성과 어떠한 상관 관계를 가지는가 관찰한 바 식이조성 차이로 오는 효소 활성은 고함수탄소 식이군에서 malic enzyme 활성만이 높았고 고지방식이군, 저함수탄소 식이군에서는 정상식이군에 비해 감소하고 알콜투여에 의한 영향은 볼 수 없었다. 간장조직내 식이에 의한 triglyceride 함량의 변화는 정상식이군에 비해 고지방식이군, 저함수탄소식이군에서는 높으나 알콜투여에 의한 효과는 고지방식이군에서만 현저한 증가를 나타냈다. 혈청내 유리지방산 농도는 알콜투여에 의해 정상식이군에서는 1.5배(倍), 저함수탄소식이군에서는 3배(倍)의 증가가 있었으며 고지방식이군에서는 차이가 없으며 고함수탄소식이군에서는 알콜투여군이 대조군에 비해 오히려 낮았다. 식이 조성및 알콜투여에 의한 혈청내 Phospholipid 함량에는 변화가 없었다. Influences of diet composition on the activities of two lipogenic enzymes, malic enzyme and ATP-citrate lyase, and on the hepatic triglyceride accumlation were investigated in rats. In addition, the effect of chronic administration of ethanol on these enzymes and hepatic triglyceride accumulation were studied. The results were summarized as fallowing. 1) The activity of malic enzyme in the liver of rat fed high-fat (HF), low-carbohydrate (LC) and high-carbohydrate (HC) diet groups was 62%, 55% and 155% of that of the normal level, and the ATP-citrate lyase activity was 22%, 31% and 44% of that of normal level, respectively. These changes of the lipogenic enzyme activities were not altered by chronic administration of ethanol to the rats. 2) Average content of triglyceride in the liver of rat in normal diet group was 9.27mg per g. of liver, 16.37mg in the normal diet plus ethanol treated group, 37.79mg in the HF diet group, 75.7mg in the HF plus ethanol treated group, 14.78mg in the LC diet group, 17.87mg in the LC diet plus ethanol treated group, 11.61mg in the HC group, 14.92mg in the LC diet plus ethanol treated group. These results, shows that the administration of ethanol was increased hepatic triglyceride content only in the high fat diet group. 3) Free fatty acid concentration was increased in the serum of rats free fatty acid concentration was decreased by 40% in the high carbohydrate group, may be due to the increased or decreased mobilization of lipid from peripheral adipose tissue. 4) No total phospholipid content in the serum of rat was observed among above different diet and plus alcohol treated group. It is therefore apparent that ethanol administration induces the hepatic accumulation of triglyceride in the HF diet group, whereas it reduces the hepatic accumulation of triglyceride and serum free fatty acid level in the HC diet group.

백서에서 L-Tryptophan의 과량 투여시 간장내 Triglyceride 축적에 미치는 호르몬의 효과

조무연,김윤수,Cho, Moo-Youn,Kim, Yoon-Soo 생화학분자생물학회 1978 한국생화학회지 Vol.11 No.1

L-tryptophan을 백서 복강내 과량 (0.34m mole) 투여시 간장내 지방축적에 있어서 호르몬의 작용을 관찰한 바 부신적출한 백서에서 Epinephrine과 L-tryptophan 동시주사군이 Epinephrine 단독주사군에 비하여 1.5배의 증가를 보이고 혈액내 유리지방산의 농도도 높으나 그외의 다른 호르몬(Hydrocortisone, Glucagon, Insulin)은 간장조직내 지방축적에 아무 작용도 없었다. When an excess amount of L-tryptophan (0.34mmole) was administered intraperitioneally to a normal rat the triglyceride content in the liver increased two times that of the normal control in three hours, but no significant changes of triglyceride content in the liver of adrenalectomized rat was noted, however, the triglyceride content in liver and FFA in the plasma of adrenalectomized rat administered epinephrine and L-tryptophan together was increased as much as one and a half times than that of the control adrenalectomized rat which received L-tryptophan or epinephrine only. No effects of hydrocortisone, glucagon and insulin on the triglyceride accumulation in the liver were noted in adrenalectomized rat receiving each hormone or L-tryptophan only, or hormone and L-tryptophan together. Therefore, it was confirmed that the triglyceride accumulation in the liver induced by L-tryptophan is due to the indirect effect of L-tryptophan through stimulation of epinephrine secretion.

배양 뼈에 있어서 Calcium 대사에 대한 Hydrocortisone 의 효과

조무연,송정석 생화학분자생물학회 1978 BMB Reports Vol.6 No.3

Cruciate Paralysis를 동반한 치상돌기 골절에 의한 제1경추골 탈구

조무연,신종하,최천식,주문배 대한신경외과학회 1991 Journal of Korean neurosurgical society Vol.20 No.7

Cruciate paralysis, an unusual clinical entity, is "frequently undiagnosed or misunderstood", and can simulate the acute cervical central-cord injury syndrome. This rare injury pattern is characterized by weakness of upper extremities with little of no compromise of lower limb function following trauma to the superior cervical cord. The patterns of injury and clinical findings support selective damage to the corticospinal tract or upper motor neurons subserving upper limb function with the pyramidal decussation. Authors experience a case of cruciate paralysis due to type 2 odontoid fracture and atlas dislocation.

Dehydro - L - ascorbic Acid 가 백서 간에서 Vitamin A 대사에 미치는 영향

조무연,김현옥,송정석 ( Moo Youn Cho,Hyun Ock Kim,Chung Suk Song ) 생화학분자생물학회 1976 BMB Reports Vol.9 No.2

The effect of dehydro-L-ascorbic acid (DHA) on the transfer of vitamin A to the liver was observed in the isolated rat liver perfusion. DHA had no effect on the transfer of vitamin A to the liver. The addition of DHA to the perfusate did not raise the level of either oxidized or reduced form of vitamin A in the liver. From this fact, DHA seems to be destroyed as soon as it is absorbed into the liver or not to be absorbed to the liver and rather to be destroyed in the blood.

CHROMOSOMAL SUBFRACTION에서 BENZO(A)PYRENE 화합물의 생화학적 연구

조무연,이송재,정인철,정인권,노준 고신대학교(의대) 고신대학교 의과대학 학술지 1990 고신대학교 의과대학 학술지 Vol.6 No.2

The distribution of the carcinogen within the chromosomal subfractions of the transformable C3H/10T1/2 mouse fibroblast cell demonstrated a significant enrich of benzo(a)pyrene(BP) in the portion of the chromatin which is complexed with the tightly bound nonhistone chromosomal proteins(DNA-P). In the chromatin isolated from cells exposed to BP, BP metabolites were stimulated to ability of binding formed DNA-protein complex compared with control. In addition, fluorometry data treated BP in C3H/10T1/2 cells clearly indicates that the covalently bound adduct is BP-diol epoxide in guanidium chlonde dissociation and proteinase K digests data indicate that the majority of the carcinogen is bound to protein rather than to DNA. The data suggest that the binding of BP in this fraction may lead to change the replicative and transcribe activities.

Doxorubicin이 HL-60 사람 백혈병 세포내 topoisomerase의 활성과 발현에 미치는 영향

조무연,정인철 고신대학교(의대) 고신대학교 의과대학 학술지 2004 고신대학교 의과대학 학술지 Vol.19 No.1

Background : DNA topoisomerases are essential enzymes present in all organisms. They modify DNA topology in connection with a number of nuclear processes, such as replication, transcription, chromatin remodeling, chromatin condensation/decondensation, recombination and repair. In the strand-breakage reaction by a DNA topoisomerase, a tyrosyl oxygen of the enzyme attacks a DNA phsphorus, foriming a covalent phosphotyrosine link and breaking a DNA phosphodiester bond at the same time. DNA topoisomerases have been shown to be the molecular targets of many antimicrobial and anticancer agents. Among topoisomerase-targeting drugs in clinical use at present, most act by trapping the covalent DNA-enzyme intermediates to convert a normal cellular enzyme to a DNA damaging agent. This studies were designed to elucidate whether pretreatment of HL-60 human leukemia cells with the topoisomerase 1-directed drug doxorubicin would increase the expression of topoisomerases and to investigate the activity of directed drug doxorubicin would increase the expression of topoisomerases and to investigate the activity of topoisomerase mediated by doxorubicin in nuclear extract from HL-60 human leukemia cells. Methods : We have conducted experiments on topoisomerase assay using gel electrophoresis, pUC-X 1 cloning, DNA sequencing, cell cytotoxicity in drug-treated cells, topoisomerase purification, quantitative RT-PCR analysis, northern blotting techniques, respectively. Results : Doxorubicin inhibited the relaxation activity of topoisomerase in pUC19 DNA at various concentrations (0.4-50uM), while it enhanced the cleavage of topoisomerase in the pUC-X 1 by forming a cleavable complex at 0.4-2uM. The levels of the topoisomerase 2a mRNA from RT-PCR analysis and northern blot were increased in HL-60 cells treated with doxorubicin, whereas the expression of topoisomerase 1 and 2b mRNA from RT-PCR analysis remained no significant change. Conclusion : Our results suggest that overexpression of topoisomerase 2a mRNA by topoisomerase 2-directed drug treatment is due to stabilizing drug-enzyme-DNA "cleavable complex".