Studies on the Activation Mechanism of c-src Protein Tyrosine Kinase by Ginsenoside-Rg1

Hong, Hee Youn,Yoo, Gyurng Soo,Choi, Jung Kap 전남대학교 약품개발연구소 1998 약품개발연구지 Vol.7 No.1

We have studied an activation mechanism of pp60^(c-src) protein tyrosine kinase (PTK) by ginsenoside-Rg₁ (G-Rg₁) in NIH(pMcsrc/foc)B c-src overexpressor cells. It was previously reported that G-Rg₁ stimulated the activation of c-src kinase at 20 μM with a 18 hr-incubation, increasing the activity by 2-4-fold over that of untreated control, and this effect was blocked by treatments of inhibitors of either protein synthesis (cycloheximide) or RNA synthesis (actinomycin D) (Hong. H.Y. et al. Arch. Pharm. Res. 16, 114 (1993)). However, an amount of c-src protein itself in wild-type cells was not changed by G-Rg₁. When the cells mutated at one or two tyrosine residue(s) (Y416/527) that are important sites to regulate the kinase activity were treated with G-Rg₁, increases both in the activity of c-src kinase and in the expression of the protein were not observed. In addition, removal of extracellular calcium ion by EGTA or inhibition of PKC by H-7 canceled the G-Rg₁-induced activation of the kinase. Although the activation was little affected by G-Rg₁ with a calcium ionophore A23187, it was synergistically stimulated by treatment of G-Rg₁ and PMA, a PKC activator. Taken together, these results suggest that the activation of c-src kinase by G-Rg₁ is caused by an increase in the specific activity of the kinase, but not in amount of it, and is involved with both cellular calcium ion and PKC. Further the increase in the specific activity of c-src kinase may result from altered phosphorylation at tyr-416 and -527.

교잡종 찰옥수수에 대한 연구 III : Ⅲ. 국내 옥수수 유전자원 수집종에 대한 주요 특성 분포

서정은,주현민,최윤표,홍범용,조양희,주정일,지희정,이희봉 충남대학교 농업과학연구소 2007 농업과학연구 Vol.34 No.2

2006년 국내 각 지역으로부터 수집된 재래종옥수수 유전자원을 관행방법에 의해 파종하고 주요특성별로 분포도를 살펴본 결과는 다음과 같다. 1. 수집된 유전자원에 대한 발아율은 대부분 80∼100%이상의 분포를 보였으나, 일부 수집계통에는 30% 미만의 극히 저조한 발아율을 보였다. 2. 수집자원에 대한 초기생육을 살펴보면 극히 생육이 저조한 것이 40% 정도를 보였고, 극히 우수한 자원은 3%미만으로 나타났다. 3. 간장은 대부분이 150∼199cm범위에 55%가 분포하고 있었으나, l00cm이하가 5%, 250cm이상이 8%로 나타나 수집종간에 큰 차이를 보였다. 4. 주당 분얼수는 1개 미만이 40%의 분포를 보였으나, 일부 수집종에서 2 내지 3개의 분얼수를 보이는 계통이 확인되었다. 5. 개화소요일수는 61∼64일의 범위에서 40%, 57∼60일의 범위에서 30%의 분포를 보였으며, 특히 일부 수집종의 경우 55일 이하의 극조생종도 10% 정도의 분포를 보였다. 6. 이삭길이는 대부분 11∼15cm범위에 60%가 포함되어 있으나, 일부계통에서 5cm미만이 2%, 16∼20cm의 이삭길이도 10%가 분포하였다. Germination ratio on the 300 collected genetic resources showed 80 to 100 percentage, but those of some accessions were very low as below 30 percentage. Early growth phase of most of these lines were very poor while some collected lines were very vigor. Range of stem height showed from 150 to 199cm, but that of some accessions showed below 100cm as five percentage and some of them was over 250cm as eight percentage. Number of tillers per plant appeared less one but some collected lines have two to three tillers per plant. In days to tasseling, some accessions showed more four per plant. Ear length of these accession showed above 60 percentage as 11 to 15cm, while that of some collected lines showed over 10 percentage as 16 to 20cm.

노인의 영적 간호요구

최미혜,김경희,김귀옥,김기숙,김수강,김정신,김춘숙,노흥진,박지연,성혜연,오명선,이선희,이원옥,이윤영,이현수,장명재,차혜경,채정선,홍상희 중앙대학교 의과대학 간호학과 간호과학연구소 2001 중앙간호논문집 Vol.5 No.1

This study was designed to exam the aged's needs for spiritual nursing care. The purpose was to serve as a basis for the development of spiritual nursing practice. The major findings are as follows : 1. The degree of needs for spiritual nursing care as area was that needs of love and relationship mean 22.0, needs of meaning and object mean 28.2, needs of forgiving mean 13.5. Total needs for spiritual nursing care mean 63.7, which was on the upper middle level. The needs of meaning and object was rated highest. 2. Among the general characteristics of the subjects, needs of love and relatiohship wasn't significanlty different. 3. Among the general characteristics of the subjects, needs of meaning and object was significantly different according to two factors : age(F=7.260, p=0.001), religion(F=5.275, p=0.001). Higher needs of meaning and object was possessed by the older than the younger, by the one who have religion than the other. 4. Among the general characteristics of the subjects, four factors made a significantly difference to needs of forgiving : sex(t=-2.851, p=0.006), age(F=8.201, p=0.001), religion(F=6.928, p=0.000), disease(t=2.327, p=0.024). Higher needs of forgiving was possessed by man than woman, by the older than the younger, by the one who have religion than the other, by the one who have disease than the other.

정신분열병에 대한 리스페리돈의 효과 및 안정성

이민수,김용구,김영훈,연병길,오병훈,윤도준,윤진상,이철,정희연,강병조,김광수,김동언,김명정,김상훈,김희철,나철,노승호,민경준,박기창,박두병,백기청,백인호,손봉기,손진욱,양병환,양창국,우행원,이정호,이종범,이홍식,임기영,전태연,정영조,정영철,정인과,정인원,지익성,채정호,한상익,한선호,한진희,서광윤 大韓神經精神醫學會 1998 신경정신의학 Vol.37 No.1

연구목적 : 본 시험의 목적은 임상시험 시작전에 연구자들을 대상으로 PANSS Workshop을 통하여 PANSS, ESRS에 대한 국내에서의 표준화 작업을 구축하고 새로운 정신병 치료제인 리스페리돈의 효과와 안정성을 재확인하여 리스페리돈 사용에 대한 적정화를 이루는데 있다. 연구방법 : 1996년 4월부터 1996년 9월까지 국내 39개 대학병원 정신과에 입원중인 혹은 증상이 악화되어 입원하는 정신분열병 환자 377명을 대상으로 다시설 개방 연구를 시행하였다. 1주일간의 약물 배설기간을 가진후, 리스페리돈을 8주간 투여하였고, 기준점, 1주, 2주, 4주, 그리고 8주후에 평가되었다. 용량은 제1일에는 리스페리돈 1mg씩 1일 2회, 제2일에는 2mg씩 1일 2회, 제3∼7일에는 3mg씩 1일 2회 투여하였다. 이후 환자의 임상상태에 따라 임의로 증량할 수 있으며, 최대 일일 16mg을 초과하지 않도록 하였다. 추체외로 증상을 조절하기 위한 투약을 허용하였다. 임상증상 및 부작용의 평가는 PANSS(Positive and Negative Syndrome Scale), CGI(Clinical Global Impression) 그리고 ESRS(Extrapyramidal Symptom Rating Scale)을 사용하였다. 연구결과 : 377명중 343명(91%)이 8주간의 연구를 완결하였다. 치료 종결시점인 8주후 PANSS 총점수가 20% 이상 호전된 경우를 약물 반응군으로 정의할때, 약물반응군은 81.3%였다. 리스페리돈에 반응하는 예측인자로는 발병연령, 이전의 입원 횟수, 유병기간이 관련 있었다. 리스페리돈은 1주후부터 PANSS양성, 음성, 및 일반정신병리 점수상에 유의한 호전을 보여 효과가 빨랐다. CGI의 경우도 기준점에 비해 1주후부터 유의한 감소를 나타내었다. ESRS의 경우, 파킨슨 평가점수는 기준점과 비교해 투여 1주, 2주, 4주후 유의하게 증가되었다가 8주후 기준점과 차이가 없었다. Dystonia 평가점수는 1주후만 유의한 증가를 보였으며, dyskinesia 평가점수는 유의한 차이가 없었다. 혈압, 맥박수의 생명징후 및 일반 혈액학 검사, 생화학적 검사, 심전도 검사에서 유의한 변화는 없었다. 결 론 : 이상의 다시설 개방 임상 연구를 통해 리스페리돈은 정신분열병 환자에서 양성증상뿐만 아니라 음성증상 및 전반적인 증상에도 효과적인 것으로 사료된다. 보다 명확한 평가를 위해서는 다른 항정신병약물과의 이중맹검 연구가 필요할 것으로 생각되며, 또한 장기적 치료에 대한 평가도 함께 이루어져야 하겠다. Objective : The purpose of this study was to investigate the efficacy and safety of risperidone in the treatment of Korean schizophrenic patients. Method : This multicenter open study included 377 schizophrenic patients drawn from 39 university hospitals. After a wash-out period of 1 week, the schizophrenic patients were treated with risperidone for 8 weeks and evaluated at 5 points ; at baseline, and 1, 2, 4 and 8 weeks of treatment. The dose was increased from 2mg/day(1mg twice daily) to 6mg/day(3mg twice daily) during the first week and adjusted to a maximum of 16mg/day over the next 7 weeks according to the patient's clinical response. Medication to control extrapyramidal symptoms was permitted. The psychiatric and neurological status of the patients was assessed by PANSS, CGI, and ESRS scales. Results : 343(91%) of 377 patients completed the 8-week trial period. Clinical improvement, as defined by a 20% or more reduction in total PANSS score at end point, was shown by 81.3% of patients. The predictors of response to risperidone were associated older age, shorter duration of illness, fewer previous hospitalization. Risperidone had rapid onset of action ; a significant decrease of the total PANSS and three PANSS factor(positive, negative, general), and CGI was already noticed at the end of first week. For the ESRS, parkinsonism rating scores were significantly increased until week 4 comparing with baseline. Dystonia rating scores were significantly increased until week 1, and dyskinesia rating scores were not significantly changed during the study. Laboratory parameters including vital sign, EKG, hematological, and biochemical values showed no significant changes during the trial. Conclusions : This study suggests that risperidone is generally safe and effective against both the positive and negative symptoms in our group of patients.

鋼箱子桁 壓縮플랜지의 補强재에 대한 硏究

鄭熺孝,金洪俊 경상대학교 생산기술연구소 2002 工學硏究院論文集 Vol.2 No.-

The sufficient safe factor for buckling must be obtained by pertinent stiffness in steel box girder. In this paper, the critical stress of stiffened plate with longitudinal ribs is estimated by the classic approximate method of analysis, integration of the two Karman's differential equtions and the energy method which put strain energy of bending equals the work done during buckling by the compressive force. Especially, according to change of thickness of compressional flange and area of stiffening ribs, the critical load of stiffened plate was analytically investigated and these results of theory and experiment were compared with analysis program, SAP2000

舞踊史 : 西歐 Ballet 發展에 공헌한 男性舞踊家

洪禎禧 이화여자대학교 한국문화연구원 1966 韓國文化硏究院 論叢 Vol.9 No.-

It seems to scorn at male dancing and to despire male dancers in our contemporaries. Such a tendency comes not only from the womanly manners of some of the thoughtless male dancers, but also from the ignorance that they do not know that it was male who come into dance and Ballet first. In Korea, where the recognition of male dancing is not popularized yet, such a trend must be abolished for the development of our Ballet, for Ballet is a branch of dances that requires male dancing essentially. Inferring that Ballet has been motivated and promoted by man as well as woman, it is not so difficult to suppose more of masculine dancer's participation will be a help for Ballet. It is generally accepted that Ballet, even in rude form, was started at the Italian Court in the fifteenth century, an in 1661 the Royal Academy of Dancing was formed by Louis XIV, who was himself a ardent performer. In eighteenth century, based on the scientific analysis, Jean G. Noverre established various fundamental techniques and he trained many of the male dancers, and later he becomes the leading features of European Ballet. However, in nineteenth century the Realism of the previous was changed into the Romanticism and thus Ballet stressed on the female dancers under the influences of the ideal of that period. Taglioni and Elssler made their appearances and male dancing degenerated as a stage effect. In the twentieth century male dancing was survived by a great Russian, Serge Diaghilev and his trinity of dancing, music and painting made male dancing an important role. His trinity was completed in some degree by Lifar and Nijinsky and elevated Ballet as a form of synthetic arts. Besides,, With his "Ballet Russes," he aroused a new recognition in England, where is now the center of Ballet. With the male dancers who have contributed to the art of Ballet, Ballet is now on the unstable base. It may seem rather strange that the author, as female, put stress on the importance of male dancers. However, feminine beauty and elegance can be more effectively seen by a support of good male dancers and in turn sublimity of a male dance also can be a good one when the ballerina's flexibility is followed. Different from mere "dancing" which derives from the natural urge, Ballet is an artificial creation through a medium of a trained dancer. In order to carry out his or her own duties, a dancer must be equipped with necessary knowledge, culture and refinement. Especially if a male dancer wants to avoid a voluptuous carrier he should be a man of good culture. But the culture is not cultivated in a day but gradually percolated into him. Thus we have urgent need of having training schools of Ballet. Fortunately enough, we have several schools where dancing is taught, but it is more important that each sex had better be trained at a separate place and under a different method as many of the dancing teachers have said. Such an assertion springs naturally when we compare the both sexes in view of anatomy, psychology and energetics. Finally, the author hope in this article that in Korea we have urgent need to have more of male dancers with good culture for the Future of Korean Ballet.

IgG3결핍 천식 환자에서 면역 글로불린 치료로 호전된 1례

김희규,옥철호,정만홍 고신대학교 의과대학 2009 고신대학교 의과대학 학술지 Vol.24 No.2

반복적인 호흡기 감염으로 천식 악화가 재발되는 환자는 면역 결핍이 그 원인일 수 있다. 저자들은 20년 전부터 기관지 천식으로 진단받고 최근 들어 잦은 호흡기 감염과 천식 악화가 발생한 환자에서 IgG3결핍을 확인하여 면역 글로불린 투여 후 임상적으로 호전된 1례를 경험하였기에 문헌 고찰과 함께 보고하는 바이다. Asthma is precipitated into acute episode of airway obstruction by respiratory infection. Also, some immunological abnormalities including deficiencies of IgG subclasses are reported in patients with asthma. A 65 year old woman, who has a 20 years history of bronchial asthma and allergic rhinitis, has been suffering from recurrent upper and lower respiratory infection for 2 years. She has been admitted frequently by asthma exacerbation. We evaluated her immunoglobulin level. IgG subclass III level was reduced at 14.3 mg/dl (normal range 23.0~196.0 mg/dl) with other IgG subclass profiles in the normal range. So, we administrated IVIG (intravenous immunoglobulin) monthly 0.4 g/kg dose for symptomatic IgG3 deficiency. She was much improved in clinical course : without asthma exacerbation, recurrent respiratory infection, and need for antibiotics. So, the symptomatic IgG subclass deficient patients may be candidates for IVIG replacement.

A Study on Effect of the Elderly s Leisure Life upon Psychological Loneliness and Life Satisfaction and Social Support s Mediating Effect

Jung,Myung-Hee,Kim,Hong-Sun 한국유통과학회 2019 KODISA ICBE (International Conference on Business Vol.2019 No.-

This study, it is intended to obtain a theoretical understanding on the relationship among the elderly s leisure activity, social support, life satisfaction and mental loneliness while, together with this, it is also intended to draw policy implications for enhancing the psychological well-being of the elderly in a practical dimension. The objective of this study consists in doing research on the influence of leisure activity exerted upon both life satisfaction and mental loneliness of the elderly over 60 and also on the moderating role of social support. The purpose of this study was to search for the influence of leisure activity and social support being exerted upon life satisfaction and mental loneliness as well as the moderating role of social support. In this study, as leisure activities, the 2 types of productive leisure activity and consumptive leisure activity, and as social support, the 3 types of spouse support, children support and friend support, and the 2 factors of life satisfaction and mental loneliness have been reviewed. The result of this study is suggesting policy implications that are important for enhancing life satisfaction in senescent period of the elderly.

원형 Cavity를 이용한 펄스형 Nb:YAG 레이저의 출력특성 및 병렬메쉬 회로의 최적화

홍정환,송금영,양동민,김휘영,김희제 부산대학교 생산기술연구소 2001 生産技術硏究所論文集 Vol.60 No.-

본 연구에서는 기존의 타원형 cavity가 아닌 원형 cavity를 설계 및 제작하여 그 동작특성에 관하여 연구하였다. 그 결과 타원형 cavity 보다 다소 적은 레이저 출력 효율 2.1%를 얻었다. 원형 cavity는 타원형 cavity 보다 컴팩트하며 제작하기가 용이하고, 제작 단가가 저렴하다는 장점이 있다. 입력에너지, 입력전압 및 펄스 폭을 동일하게 했을 경우 주회로 병렬 메쉬회로수를 최적화하고자 한다. PFN 회로를 1단에서 8단까지 PSPICE로 모의시험한 전류파형과 실제 실험한 전류파형이 거의 일치하였다. 그 결과 500㎲이상의 펄스 폭에서 6단 메쉬가 최적의 병렬 메쉬라는 결론을 얻었다. In this study, we have deigned and manufactured not only a present elliptic cavity but also a circular cavity, and experimented the operational characteristics. As a result, we obtained the maximum efficiency of 2.1% that was not less than that of an elliptic cavity. A circular cavity is much more compact and is easier to be manufactured than an elliptic cavity. And it can be made at a low cost. When the input energy, input voltage, and pulse width were in the same condition, we tried to decide the optimization of the mesh number of a parallel-mesh circuit which was connected with main power supply. The PSPICE simulation waveform of PFP(Pulse Forming network) circuit ranging from 1-mesh to 8-mesh nearly corresponded with the real current waveform obtained through a practical experiment. From these experimental results, we obtained the result that 6-mesh was the optimal parallel-mesh circuit in case that the pulse width was more than 500㎲.

상자형의 전단지연 현상과 유효폭에 관한 연구

鄭熺孝,金洪俊,李承烈,朴峻熙 경상대학교 생산기술연구소 2002 工學硏究院論文集 Vol.2 No.-

In a box girder, it is an accepted practice in structural engineering to represent the effect of 'shear lag' by adopting an 'effective width' concept. The actual width of flange plate (b) is replaced by a reduced width (b_e) over which the longitudinal stresses may be considered uniformly distributed and the application of the elementary theory of bending to the transformed girder cross section gives the correct value of maximum longitudinal stress (σ_max). This paper provides how to determine the magnitude of 'shear lag' and 'effective width ratio' in case of orthotropic box girder which is simply supported, symmetrically loaded only.