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      • Paraquat가 Bacillus megaterium 및 Saccharomyces cerevisiae의 膜 구성 지질의 지방산 조성에 미치는 영향

        金春淑,林英淑,崔慶浩 대구효성가톨릭대학교 응용과학연구소 1991 응용과학연구논문집 Vol.1 No.-

        Paraquat 처리가 미생물의 막지질 조성에 미치는 영향을 검토할 목적으로 대수증식기의 B. megateriumium 및 S. cerevisiae 배양액에 최종농도 10^-3M의 paraquart를 첨가한 후 증식저해의 정도 및 지질함량, 지방산조성을 조사하였다. Paraquat 처리에 의하여 공시한 두 균주의 증식이 현저히 저해 되었으며, 효모의 경우에도 75%의 균체가 사멸하였다. B. megaterium은 총 지질량에 유의적인 변화를 보이지 아니하였으나 S. cerevisiae는 paraquat 처리구에서 약간의 감소를 나타내었다. TLC에 의하여 지질을 분리한 결과 두 균주로부터 triglyceride, cholesterol, phospholipid 및 유리 지방산이 뚜렷이 분리되었으며 paraquat 처리에 의한 차이점은 발견되지 아니하였다. GC에 의해 막중의 총지방산을 검토한 결과 B. megaterium에서는 paraquat 처리에 의하여 steatic acid가 감소된 반면에 oleic acid가 증가되었다. S. cerevisiae의 경우에는 처리후 palmitoleic acid와 oleic acid가 감소된 반면에 palmitic acid와 stearic acid가 증가되었다. 한편, 막의 인지질 구성 지방산 조성을 검토한 결과 B. megaterium에서는 유의적인 차이가 없었으나 S. cerevisiae에서는 paraquat 처리에 의하여 stetic acid가 현저히 감소된 반면에 palmitoleic acid 함량이 증가되었다. Growing cells of a bacterium(Bacillus megaterium) and an yeast(Saccharomyces cerevisiae) were exposured to the paraquat, and then, composition of membrane lipid was assayed to understand toxic action of the herbicide against microorganisms. Paraquat repressed turbid metric growth of the both organisms. Approximately 75 percent of the yeast cells were killed after 12 hours of exposure to paraquat. B. megaterium and S. cerevisiae was composed of lipid by 2.0 and 1.6 percent of dry cell, respectively. Triglyceride, cholesterol, phospholipid and free fatty acid were separated from lipid extract of sonic disruptured cells. Paraquat treatment induced no significant difference in content and kind of the lipid. Lipid of the bacterial membrane had oleic acid as the highest fatty acid by occupying, 22.0 percent of total fatty acid. Content of the acid increased to 32.4 percent by paraquat treatment. The yeast had palmitoleic and oleic acid as prediminant fatty acid and the acid occupyed by 43.6 and 39.6 percent of total fatty acid, respectively. Differently from the bacterium content of saturated fatty acids such as palmitic and stearic acid increased near to two folds by paraquat treatment, while content of the palmitoleic and oleic acid decreased. Membrane phospholipid of the bacterum contained myristoleic and palmitic acid as the predominant fatty acid and content of the each acid was more than 20 percent. Fatty acid composition of the phospholipid showed no significant changes by paraquat treatment. Linoleic acid was the highest fatty acid of membrane phospholipid of the yeast. content of the acid was 43.2 percent. Ctearic acid decreased from 7.0 to 0.5 percent, while palmitoleic acid was increased from 18.7 to 30.1 percent by paraquat treatment.

      • 도시 노인의 폐경 후 삶

        김춘숙,최경숙 중앙대학교 간호과학연구소 2001 중앙간호논문집 Vol.5 No.2

        The purpose of this study is to explore the life of the old-aged people experiencing the aging process actuality after menopause in urban area. The present study investigates the life of old-oged people ]n the urban area by using ethnographical method such as in-depth interviews with informants and researcher's participant observation. The number of informants involved in tutus study was 14 and the old women aged 65 or more and the data collection period was from October of 2000 to February of 2001. The result of this study shows that the subject of study mostly faced menopause without preparation and could not pay enough attention to the physiological phenomenon because of economic difficulties. As a result, they became exposed to a sense of feminity loss and felt ambivalent about menopause. After menopause, their sexual activities usually stopped, and the onset of the aging process depressed them and drove them to despair. Nevertheless, they struggled to adapt themselves to a new life. In conclusion, this study found that the life of the old-aged women after menopause was noticeably different from that of before menopause. It is quite meaningful that this study shed light on social and economic factors that forced the old women to be exposed to emotional side effects of menopause without proper preparation. Therefore, the implication of nursing needs joint efforts by various organizations, including schools, hospitals, government agencies, and civic groups, to address erroneous views that menopause puts an end to the sex life. In line with this, a variety of social programs including job offers are required to help them get out of loneliness. Moreover, information on osteoporosis should be provided to them, and let them have access to diverse medical services to enjoy a better life.

      • KCI등재

        일부종합병원 신규간호사의 의사소통 경험

        김춘숙 경희대학교 경영연구원 2020 의료경영학연구 Vol.14 No.1

        The purpose of this study is to deeply understand the meaning of New Nurse's communication experience in some general hospitals. Data collection and analysis were gathered with Focus Group discussion of the qualitative research method, and the gathered data were analyzed with the data analysis method of Colaizzi(1978). The total participants were 6 New Nurse , who were divided in to two groups The total interview time was 3 hours. The students stated about 75 topics, which can be classified into 12 subjects, and then 5 categories: “Communication is ignored”, “Poor communication on the system”, “Communication difficulties”, “Conflict over nurse role” and “Leave the nurse”. In conclusion, the five categories described above show that this study aims to help the development of new nurses' educational programs, quantitative basic data, and medical management through their experiences in communication with new nurses after graduation.

      • KCI등재

        현미-채식이 고혈압, 당뇨병 환자에게 미치는 영향

        김춘숙,송충숙 경희대학교 경영연구원 2022 의료경영학연구 Vol.16 No.2

        The necessity of this study is the increase of hypertension and diabetes patients and the burden of individual, social and national burdens as they are taking medications for life. The purpose of this study was to investigate the influence of brown rice-vegetarian diet on hypertension-diabetic patients. As a research method, data were collected using the focus group discussion method of qualitative research, and the analysis method of Colaizzi (1978) was conducted. For data collection, the study participants were divided into 2 groups and interviewed with a total of 6 people from their point of view. As a result of the study, 5 categories, 11 topics, and 85 related meanings were derived. The derived categories were 'experienced physical changes', 'recognized the importance of food', 'choose brown rice-vegetarian food', 'better health', 'Stopping high blood pressure or diabetes medication'. In conclusion, the results of this study showed that as participants chose a brown rice-vegetarian diet, blood pressure and diabetes were naturally controlled, leading to discontinuation of antihypertensive and diabetes medications. Therefore, it is thought that the individual-society-national burden can be reduced by the participants quitting the drug, and from a nursing perspective, a change in perception and awareness of the importance of diet in nursing intervention for both disease groups is expected.

      • KCI등재후보

        비폭력 의사소통교육이 간호사의 의사소통능력, 의사소통 자기효능감, 스트레스에 미치는 효과

        김춘숙,이미옥,이상복 경희대학교 경영연구원 2016 의료경영학연구 Vol.2 No.1

        This study investigated the effects of nonviolent communication(NVC) program for communication competency, communication self-efficacy and Stress in 48 hospital nurses in Korea. NCV program was provided for 15 weeks. The The NVD program contents were interpersonal relationships, communication skills, NVC, role plays. Data were measured before and after the NCV program by questionnaires of Lee ets(2003)'s communication competency scale, Ayers(2005)'s communication self-efficacy scale and stress Visual Analogue Scale. Statistic analysis was done by SPSS 21 program, descriptive statistics of mean, standard deviation, minimum and maximum and paired t-test for difference analysis of the variables, The results were all statistically significant.: communication competency(p=.000), communication self-efficacy(p=.000), stress(p=.000). NVC program improved nurses/s communication competency, communication self-efficacy and lowered nurse's stress. The results will provide the basic data and evidences of NCV communication for nursing practices and further studies related NVC. The results will be used for developing more various NVC intervention programs for nurses who suffered from violence verbal abuse at work.

      • KCI등재후보

        청소년의 식이습관이 불안, 스트레스대처방안 및 학교폭력성향에 미치는 영향에 관한 연구 -중학생을 중심으로-

        김춘숙 ( Chun Sook Kim ),김지흔 ( Ji Heun Kim ) 경희대학교 경영연구원 2013 의료경영학연구 Vol.7 No.4

        This study was conducted to address the fact that dietary habits, which are important for the adolescents` mental and physical influence, can exacerbate anxiety and stress coping measures and affect propensity for school violence, with their physical-emotional-mental effects. The purpose of this study is to examine the relationship of influences that the dietary habits of adolescents have on anxiety, stress management measures and school violence tendencies. As for the method of study, with 315 male students at a middle school as the study subjects, data was collected from 4/15/2013 to 6/30/2013 to be studied via a structural equation model. Results was found that the dietary habits of adolescents are related to anxiety and stress management measures, and that the dietary habits of perpetrators of school violence were related to anxiety, and these habits exert influence on propensity for school violence. In conclusion, by exerting influence physically-emotionally-mentally, the dietary habits of adolescents exacerbated their anxiety and stress management measures and affected school violence tendencies. As such, with the results of this study as the basis, this study proposes to offer basic materials for the development of programs -in the study of nursing -for character education and school violence prevention programs.

      • KCI등재후보

        비폭력 의사소통교육이 간호사의 의사소통능력, 의사소통 자기효능감, 스트레스에 미치는 효과

        김춘숙 ( Kim Chun Sook ),이미옥 ( Lee Mi Ok ),이상복 ( Lee Sang Bok ) 경희대학교 경영연구원 2016 의료경영학연구 Vol.10 No.3

        This study investigated the effects of nonviolent communication(NVC) program for communication competency, communication self-efficacy and Stress in 48 hospital nurses in Korea. NCV program was provided for 15 weeks. The The NVD program contents were interpersonal relationships, communication skills, NVC, role plays. Data were measured before and after the NCV program by questionnaires of Lee ets(2003)`s communication competency scale, Ayers(2005)`s communication self-efficacy scale and stress Visual Analogue Scale. Statistic analysis was done by SPSS 21 program, descriptive statistics of mean, standard deviation, minimum and maximum and paired t-test for difference analysis of the variables, The results were all statistically significant.: communication competency(p=.000), communication self-efficacy(p=.000), stress(p=.000). NVC program improved nurses/s communication competency, communication self-efficacy and lowered nurse``s stress. The results will provide the basic data and evidences of NCV communication for nursing practices and further studies related NVC. The results will be used for developing more various NVC intervention programs for nurses who suffered from violence verbal abuse at work.

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