Slide Session : OS-IFD-07 ; Infectious Disease : In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus

( Myung Jin Lee ),( Kye Hyung Kim ),( Jong Youn Yi ),( Su Jin Choi ),( Chung Jong Kim ),( Nak Hyun Kim ),( Kyoung Ho Song ),( Pyoeng Gyun Choi ),( Ji Hwan Bang ),( Wan Beom Park ),( Eu Suk Kim ),( San 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1

In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus Myung Jin LEE1, Kye-Hyung KIM1, Jongyoun YI2, SuJin CHOI1, Chung-Jong KIM1, Nak- Hyun KIM1, Kyoung-Ho SONG1, Pyoeng Gyun CHOI1, Ji-Hwan BANG1, Wan Beom PARK1, Eu Suk KIM1, Sang-Won PARK1, Hong Bin KIM1, Nam Joong KIM1, Myoung- Don OH1 Seoul National University College of Medicine, Korea1, Pusan National University School of Medicine, Korea2 Background: Severe fever with thrombocytopenia syndrome (SFTS) is an emerging infectious disease caused by a novel Bunyavirus, severe fever with thrombocytopenia syndrome virus (SFTSV). No effective antiviral therapy is proven yet, but clinical use of ribavirin (RBV) has been tried. We investigated the antiviral effect of RBV against SFTSV in vitro. Methods: To test for cytotoxicity of RBV, Vero cells were treated with different concentrations of RBV (3.90 to 500 μg/mL, two-fold dilution) and analyzed by cell viability MTS assay 48h post-infection. To determine antiviral activity of RBV against SFTSV, Vero cells were infected with SFTSV strain Gangwon/Korea/2012 at 100 TCID50 (50% tissue culture infective dose) per well in a 96-well plate, and RBV was added at the concentrations showing no or minimal cytotoxicity. Viral RNAs were extracted from the culture supernatants and quantifi ed using one-step real-time reverse transcription- PCR to amplify the partial large segment of SFTSV. Statistical analysis was done by one-way ANOVA with Tukey`s post hoc test. Results: Cytotoxicity due to RBV was not observed at RBV concentration =31.3 μg/ mL. Viral RNAs at 24h post-RBV treatment were reduced with increasing RBV concentrations (1-32 μg/mL), compared with those of mock-treated cells (P <0.01, Figure). Half maximal inhibitory concentration (IC50) of RBV was 3.69 μg/mL at 24h post-RBV treatment. Conclusions: Our study shows that RBV has antiviral effect against SFTSV in a dose-dependent manner. Further studies are required to evaluate the effi cacy of RBV in SFTS.

Group Size And Measure Of Group-Level Properties

Kim,Kyoung-Su,Kim In-Sook,Jun,Gyung-Ju,Yang,Dong-Min 한국인적자원관리학회 2009 인적자원관리연구 Vol.16 No.4

Bliese와 Halverson(1998)은 집단수준의 효과를 분석하는데 있어서 기존에 사용되어 온 eta-squared 보다는 eta-squared 값이 사용되어야만 한다는 것을 주장하였다 그러나 이들이 제시한 수정된 eta-squared 접근은 다음과 같은 한계점을 가지게 되는 바 첫째, 이 접근법은 개인수준의 데이터를 집단 수준의 데이터로 만드는데 있어서의 정당성을 제공하는 기준을 구체적으로 제시하고 있지 못하다는 것을 들 수 있고, 둘째, 이 접근법은 또한 집단 내 효과를 용인하고 분석하는 기준을 제공하고 있지 못하다는 점을 들 수 있다 본 논문에서는 이러한 한계점을 극복할 수 있는 몇 가지의 대안을 제시하고 있으며, 이와 더불어 개인수준의 데이터를 집단 수준으로 만드는 것에 대한 정당성을 위한 또 하나의 추가적인 절차를 제시하였다 Bliese and Halverson(1998) argue that corrected eta-squared, rather than eta-squared, should be used for analyzing group-level effects However, their proposed corrected eta-squared approach has the following limitations (1) it fails to specify a criterion for justifying the aggregation of individual-level data to the group level and (2) it fails to specify a criterion for analyzing and allowing for within-group effects In this paper, we suggest several alternatives that overcome these limitations We suggest an additional procedure for justifying the aggregation of individual-level data to the group level

광주지역 소아에서 유행한 호흡기바이러스 유행양상 분석

서진종,김민지,김선희,기혜영,정재근,김은선,박종태,김경심,이수야,김명권,정윤석 대한감염학회 2008 감염과 화학요법 Vol.40 No.4

Background : This investigation was to perform the epidemiological surveillance and genetic analysis on respiratory viral agents from children with acute respiratory infections in Gwangju. Materials and Methods : For this study, 3,695 specimens obtained from patients with acute respiratory infections were collected by collaboration with pediatric hospitals in Gwangju between 2005 and 2007. Specimens were screened for 8 respiratory viruses including influenza viruses (IFV), human rhinoviruses (HRV), human coronaviruses (HCoV), adenoviruses (ADV), parainfluenza viruses (PIV), human enteroviruses (HEV), respiratory synthitial viruses (RSV) and human bocaviruses (HBoV). Respiratory viruses were detected using multiplex (RT) PCR with viral specific primers. Results : Out of 3,695 specimens, the ratio of virus detection was 24.9% (919). Overall, HRV (35.5%) and IFV (34.9%) were the most common viruses found, followed by HBoV (14.8%), HCoV (10.6%), RSV (3.7%), ADV (3.4%), PIV (3.2%) and HEV (3.0%). In addition, multiple infections were detected in 80 patients (8.7%). When the prevalence was analyzed according to season, HBoV, IFV and HCoV showed two epidemic points in late spring and early winter. ADV, HRV, RSV PIV and HEV, however, were all found to have only one epidemic point, with RSV being most common during winter and the others being most prominent during spring. Conclusions : Through this epidemiological surveillance, the respiratory viruses prevalent in children in Gwanaju area were investigated. We strongly recommend the development of nationwide policy for the management of prevalent respiratory virus that includes long term collection of data and samples, vaccine development and prevention education of the misuse of antibiotics.

학교 직업교육이 장애인의 취업 및 임금에 미치는 영향

김동일,김수정,김경선 한국장애인고용공단 고용개발원 2012 장애와 고용 Vol.22 No.3

본 연구는 학교에서 제공하는 직업교육 프로그램이 실제 장애인의 졸업 후 취업과 임 금에 영향을 미치는지 여부를 탐색하였다. 이를 위해 장애인고용패널 2008년도 데이터 를 사용하여 분석을 수행하였다. 장애인의 특성을 인구학적 변인, 노동환경 변인, 인적자 원변인으로 나누어 학교 직업교육여부에 따른 취업효과와 임금효과를 로지스틱 회귀분석 과 다중회귀분석을 통해 수행하였으며, 그 결과 학교 직업교육의 취업효과와 임금효과는 나타나지 않는 것으로 확인되었다. 통제변수의 경우 남성이며 기혼이고 청각장애일수록, 장애가 경증일수록, 건강상태가 좋으며, 중소도시와 농어촌에 거주할 경우 취업률이 높아 지는 것으로 나타났다. 반면 장애정도에 따라 직업교육의 효과가 다르게 나타날 수 있으 므로 직업교육 및 장애유형과 장애정도의 상호작용항을 삽입한 결과 직업교육과 장애등 급과의 상호작용항이 취업여부에 유의한 영향을 미치는 것으로 나타났다. 임금효과를 알 아보기 위한 다중회귀분석에서도 학교 직업교육 이수 여부에 따른 임금효과는 유의미하 지 않은 것으로 나타났다. 통제변수의 경우 인구학적 특성으로는 성별, 결혼여부, 장애등 급, 건강상태가, 노동환경 특성으로는 사업체 근로자수와 정규직 여부가, 인적자본 특성 으로는 경력과 자격증보유여부가 임금에 유의미한 영향력이 있는 변인으로 나타났다. This study examined the effect of the vocational Education on the probability of obtaining job and the amount of salary for individual with disabilities. The data analyzed was extracted from Panel Survey of Employment for the Disabled and released by Korean Employment Agency for the Disabled. Logistic regression and multiple linear regressions were used to analyze the effectiveness of vocational education. The results of this study are as follows; First of all, the vocational education experience has no statistical significant impact on both the probability of obtaining job and the amount of salary. For control variables, males, individuals who has hearing impairment rather than physical disability and visual impairment, individuals with mild disability compare to those who have severe disability, individuals with good health, individuals who lived in small city compare to those in big cities has more probability of obtaining job. Control variables such as gender, marital status, disability level, work conditions, working experience, and license or certification were the variables which had casual effect on the amount of salary.

시멘틱 웹 기반의 비교구매 에이전트를 위한 동적 웹 온톨로지 시스템에 대한 연구

김수경,안기홍 한밭대학교 정보통신전문대학원 2005 정보통신전문대학원 논문집 Vol.3 No.1

기존 전자상거래 플랫폼과 컨텐츠는 데이터의 확장 및 통합이 고려되지 않은 HTML을 중심으로 한 표현 기반 기술로 되어 있고, 특히 전자상거래 사이트별로 상품 정보에 대한 분류체계가 상이함으로 인해 구매자에게 상품별 비교와 검색에 있어서 많은 시간이 낭비되고 또한 정보의 공유가 어려운 기술로 인하여 판매자와 구매자 모두의 요구를 만족시키지 못하고 있다. 본 논문에서는 최근 차세대 웹 기술로 각광받고 있는 시맨틱 웹 기반 기술인 RDF/RDFS를 이승하여 기존 이종의 상점에 제시된 상품 정보를 RDF 문서로 생성하고, Web Ontology Language(OWL)를 사용하여 상품 지식 기반 온톨로지를 구축한 뒤, RDF 문서와의 분석과 매칭을 통하여 이종의 상점에 표현된 상품을 실시간으로 비교 검색하고 동적으로 상품에 대한 지식 기반 온톨로지를 생성하는 웹 온톨로지 시스템을 설계 제안하였다. The existing e-commerce platform and contents are made of expression-based technology HTML that expansion and integration of data weren't considered. Besides, a lot of time is wasted in comparison and a retrieval of product to a buyer, because a classification System about product information is different every electronic commerce site, and cannot satisfy demand of seller and buyer all by trouble of information sharing an technology. In this paper, use the RDF/RDFS which is Semantic Web-based technology to get attention with next generation Web technology recently and it generates product information of the existing store in a RDF document, and after use OWL, and establishing product knowledge-based ontology, and through analysis and matching with a RDF document, A design proposed a System that compare a product expressed to an each e-commerce site by real time, and to retrieval and redefine product knowledge-based ontology as dynamic.

건축공사시방서에서의 KS규격의 국제인증방안에 관한 연구

김경식,이재섭,김용수,이규진,조용훈,최완철,전재열,이현수,구재동,이기환 대한건축학회 2004 대한건축학회 학술발표대회 논문집 - 계획계/구조계 Vol.24 No.1

The purpose of this research is make out construction specifications for International Construction. Using off-shore products incur cost increase and longer delivery term of construction. and inconvenient on International Construction. Expand the range of additional local products conforming to performance. Life Cycle Cost. maintainability. operability. user's acceptability, etc.. And this study is suggest the standard formats and the techniques for construction specification.

뇌졸중 환자의 기능 회복을 위한 치료적 운동 적용

김수경,이재신 한국스포츠리서치 2003 한국 스포츠 리서치 Vol.14 No.6

This study is to review the application of therapeutic exercise for functional recovery in stroke patients. Peoples with stroke increase in korea and a almost all the stroke patients requires to serve early rehabilitation treatment program after onset. Stroke is syndrome characterized by inability to performance of functional activities include activities of daily living, work and leisure due to hemiplegic deficit of sensory and motor function, impaired cognition and perception, aphasia, apaxia, dysaphgia and psychosocial problem. Main treatment program is therapeutic exercise for sensorimotor function. Traditional sensorimotor approach are PNF(proprioceptive neuromuscular facilitation), Bobath's NDT (Neurodevelopmental treatment), Brunnstrom's movement therapy and Rood approach. The goal of therapeutic exercise program with stroke is functional recovery and improvement of life quality.

트레드밀 운동시 흡기근 테이핑이 폐활량에 미치는 영향

김민지,신수영,송월섭,조수진,최동락,황미진,황진규,박진현,김경,Dennis W. Fell 대구대학교 특수교육재활과학연구소 2011 再活科學硏究 Vol.29 No.1

이 연구는 트레드밀 운동과 키네시오 테이핑의 효과에 따른 폐활량의 변화를 알아보기 위해 26명의 비흡연자가 참가하였으며 키네시오 테이핑을 적용하지 않은 그룹과 키네시오 테이핑을 적용한 두 그룹으로 무작위로 배정하고 각 군들을 주 3회 6주간의 트레드밀 훈련을 실시하였다. 실험 전과 후, 스파이로미터를 사용하여 키네시오 테이핑의 적용 따른 폐활량 변화 효과를 측정하였다. 이 연구의 결과를 종합해보면 키네시오 테이핑을 적용 그룹에서 적용하지 않은 그룹에 비해 FVC, FEV1에서 유의한 증가를 보였다. 이러한 결과는 건강한 성인에서 키네시오 테이핑을 적용한 경우 키네시오 테이핑을 적용하지 않고 트레드밀 운동을 한 경우보다 폐활량의 향상에 효과적이라고 생각되어진다. The purpose of this study was to investigate the variation of vital capacity(VC) according to the effects of kinesio taping with treadmill exercise. Twenty-six non-smokers were participated in this research and these subjects were randomly assigned into two groups. To measure the VC variation effects of kinesio taping, spirometer was used. The collected data were analyzed statistically by using a paired Mauchly test and repeated measure ANOVA. The results of this study were as follows; A group: treadmill with kinesio taping B group: treadmill without kinesio taping. Between A and B, there were significant differences. In the case of A group, there was 11.66% increase of VC, during 3 weeks experiment(p<.01). In the case of B group, there were significant differences, 3.35% increase of VC, during 3 weeks taping intervention(p<.01). After 6 weeks experiment, the improvement of VC shown a significant difference with intergroup (p<.05). From this result, it was revealed that treadmill exercise with kinesio taping was effective to improve VC to healthy adult than treadmill exercise without kinesio taping.

남자 대학생의 음주 형태 변화에 영향을 미치는 유전적, 심리사회적 요인

김시경,이수경,김민경,이상익 大韓神經精神醫學會 2007 신경정신의학 Vol.46 No.4

Objectives : This study aimed to determine the general predictive factors of change in drinking behavior and to provide materials for preventing drinking problems during early adulthood through examining genetic and psychosocial factors affecting the change of drinldng behavior in college students. Methods : The subjects were 101 male college students, apart of 534 students who had completed the previous study in 2000. In the present study as a 6-years follow up, we reassessed the drinking pattem and psychosocial variables and compared the results with previous data of the same subjects. To identify factors affecting the current drinking pattem, we used stepwise multiple regession and logistic regression analysis. Results : D allele (ALDH2) was found to reduce the degree of drinking and suppress problematic drinking, and C allele (TPH) had a suppression effect on problematic drinking. Drinking motive had a direct effect on the degree of drinking and Pro-blematic drinking. Negative cognitive expectancy had a direct effect on problematic drinking. Conelusion : Authors found some factors affecting the change of alcohol drinking behavior in college students and confirmed that there were hierarchies of significance among these factors. These may be applicable as variables for predicting drinking behavior in early adulthood.

Benzoyl peroxide의 환경에서의 초기 위해성 평가

김미경,배희경,김수현,송상환,구현주,박광식,이문순,전성환,나진균 한국환경독성학회 2004 환경독성보건학회지 Vol.19 No.1

Benzoyl peroxide is a High Production Volume Chemical, which is produced about 1,375 tons/year in Korea as of 2001 survey. The substance is mainly used as initiators in polymerization, catalysts in hte plastics industry, bleaching agents for flour nad medication for acne vulgaris. In this study, quantitative Structure-Activity Relationships(QSAR) are used for getting adequate information on the physical-chemical properties of this chemical. And hydrolysis in water, acute toxicity to aquatic and terrestrial organisms for benzoyl peroxide were studied. The physical-chemical properties of benzoyl peroxide were estimated as followed; vapor pressure=0.00929 pa, Log K_(ow)=3.43, Henry's Law constant=3.54×10^(-6) atm-m³/mole at 25℃, the half-life of photodegradation=3days and bioconcentration factor(BCF)=92. Hydrolysis half-life of benzoyl peroxide in water was 5.2hr at pH 7 at 25℃ and according to the structure of this substance hydrolysis product was expected to benzoic acid. Benzoyl peroxide has toxic effects on the aquatic organisms. 72 hr-ErC_(50)(growth rate) for algae was 0.44mg/L, 48hr-EC_(50) for daphnia was 0.07mg/L and the 96hr-LC_(50) of acute toxicity to fish was 0.24mg/L. Acute toxicity to terrestrial organisms(earth worm) of benzoyl peroxide was low(14 day-LC_(50)=>1,000mg/kg). Although benzoyl peroxide is high toxic to aquatic organisms, the substance is not bioaccumulated because of the rapid removal by hydrolysis (half life=5.2hr at pH 7 at 25℃) and biodegradation(8.% by BOD after 21days). The toxicity observed is assumed to be due to benzoyl peroxide rather than benzoic acid, which shows much lower toxicity to aquatic organisms. One can assume that effects occur before hydrolysis takes place. From the acute toxicity value of algae, daphnia and fish, an assessment factor of 100 was used to determine the predicted no effect concentration(PNEC). The PNEC was calculated to be 0.7㎍/L based on the 48hr-EC_(50) daphnia(0.07mg/L). The substance shows high acute toxicity to aquatic organisms and some information indicates wide-dispersive use of this substance. So this substance is a candidate for further work, even if it hydrolysis rapidly and has a low bioaccumulation potential. This could lead to local concern for the aquatic environment and therefore environmental exposure assessment is recommended.