Slide Session : OS-IFD-07 ; Infectious Disease : In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus

( Myung Jin Lee ),( Kye Hyung Kim ),( Jong Youn Yi ),( Su Jin Choi ),( Chung Jong Kim ),( Nak Hyun Kim ),( Kyoung Ho Song ),( Pyoeng Gyun Choi ),( Ji Hwan Bang ),( Wan Beom Park ),( Eu Suk Kim ),( San 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1

In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus Myung Jin LEE1, Kye-Hyung KIM1, Jongyoun YI2, SuJin CHOI1, Chung-Jong KIM1, Nak- Hyun KIM1, Kyoung-Ho SONG1, Pyoeng Gyun CHOI1, Ji-Hwan BANG1, Wan Beom PARK1, Eu Suk KIM1, Sang-Won PARK1, Hong Bin KIM1, Nam Joong KIM1, Myoung- Don OH1 Seoul National University College of Medicine, Korea1, Pusan National University School of Medicine, Korea2 Background: Severe fever with thrombocytopenia syndrome (SFTS) is an emerging infectious disease caused by a novel Bunyavirus, severe fever with thrombocytopenia syndrome virus (SFTSV). No effective antiviral therapy is proven yet, but clinical use of ribavirin (RBV) has been tried. We investigated the antiviral effect of RBV against SFTSV in vitro. Methods: To test for cytotoxicity of RBV, Vero cells were treated with different concentrations of RBV (3.90 to 500 μg/mL, two-fold dilution) and analyzed by cell viability MTS assay 48h post-infection. To determine antiviral activity of RBV against SFTSV, Vero cells were infected with SFTSV strain Gangwon/Korea/2012 at 100 TCID50 (50% tissue culture infective dose) per well in a 96-well plate, and RBV was added at the concentrations showing no or minimal cytotoxicity. Viral RNAs were extracted from the culture supernatants and quantifi ed using one-step real-time reverse transcription- PCR to amplify the partial large segment of SFTSV. Statistical analysis was done by one-way ANOVA with Tukey`s post hoc test. Results: Cytotoxicity due to RBV was not observed at RBV concentration =31.3 μg/ mL. Viral RNAs at 24h post-RBV treatment were reduced with increasing RBV concentrations (1-32 μg/mL), compared with those of mock-treated cells (P <0.01, Figure). Half maximal inhibitory concentration (IC50) of RBV was 3.69 μg/mL at 24h post-RBV treatment. Conclusions: Our study shows that RBV has antiviral effect against SFTSV in a dose-dependent manner. Further studies are required to evaluate the effi cacy of RBV in SFTS.

심리적 안전이 창의성에 미치는 영향: 성장마인드셋과 조직지원인식의 매개효과를 중심으로

최우수(Choi, Woo-Su),오상진(Oh, Sang-Jin) 한국경영교육학회 2020 경영교육연구 Vol.35 No.5

[연구목적] 본 연구는 불확실한 경영 환경 속 기업에게 혁신 수단으로서 창의성을 제시하기위해, 심리적 안전이 창의성에 미치는 영향과 그 과정에서 성장마인드셋, 조직지원인식과의 관계를 실증하여, 창의성 제고를 위한 실용적 방안을 제시하고자 한다. [연구방법] 국내 기업에 근무하는 다양한 업종의 구성원 총 420명에게 설문지를 배부하여 353명에게 자료를 수집하였다. 심리적 안전, 창의성, 성장마인드셋, 조직지원인식과 관련된 이론 및 선행 연구를 토대로 가설을 설정하였으며, 통계 도구로는 CFA를 통해 신뢰도와 타당성을 검증하였고, 가설검증을 위해 SPSS25, AMOS25, Macro Process3.0을 사용하였다. [연구결과] 연구 모형에 대한 가설 검증 결과 심리적 안전은 성장마인드셋, 조직지원인식, 창의성에 유의한 정(+)의 영향을 주는 것으로 나타났으며, 성장마인드셋과 조직지원인식의 매개효과도 유의한 것으로 나타났다. [연구의 시사점] 본 연구는 기업 현장의 조직문화 요인과 창의성 간의 관계를 동기부여 이론으로 검증했다는데 의의가 크다. 특히, 창의성을 특별한 재능 소유자의 전유물이 아니라 조직 문화의 변화를 통해 구성원들에게 확산시킬 수 있는 혁신의 도구로 제안했다는 점에서 시사점이 있다. [Purpose] This study aims to present creativity as a means of innovation to companies in uncertain business environment, demonstrate the impact of psychological safety on creativity and its relationship with growth mind-set and perceived organizational support in the process, and suggest practical measures to enhance creativity. [Methodology] Data were collected from 353 members. Hypothesis was set based on theories and previous studies related to psychological safety, perceived organizational support, growth mind-set and creativity. The collected data were reviewed for reliability and validity through CFA, and hypothesis verification was performed using SPSS 25, AMOS 25, and Macro Process 3.0 by Bootstrap. [Findings] Psychological safety was found to have a significant positive effect on growth mind-set, perceived organizational support and creativity, and the mediating effects of growth mind-set, perceived organizational support were also significant. [Implications] This study is significant in that it has verified the relationship between organizational culture factors and creativity at corporate sites with the theory of motivation. In particular, creativity is not the exclusive property of a particular talent owner, but it has implications for spreading to members through changes in organizational culture and, through this, proposed as a tool for innovation.

트레드밀 운동시 흡기근 테이핑이 폐활량에 미치는 영향

김민지,신수영,송월섭,조수진,최동락,황미진,황진규,박진현,김경,Dennis W. Fell 대구대학교 특수교육재활과학연구소 2011 再活科學硏究 Vol.29 No.1

이 연구는 트레드밀 운동과 키네시오 테이핑의 효과에 따른 폐활량의 변화를 알아보기 위해 26명의 비흡연자가 참가하였으며 키네시오 테이핑을 적용하지 않은 그룹과 키네시오 테이핑을 적용한 두 그룹으로 무작위로 배정하고 각 군들을 주 3회 6주간의 트레드밀 훈련을 실시하였다. 실험 전과 후, 스파이로미터를 사용하여 키네시오 테이핑의 적용 따른 폐활량 변화 효과를 측정하였다. 이 연구의 결과를 종합해보면 키네시오 테이핑을 적용 그룹에서 적용하지 않은 그룹에 비해 FVC, FEV1에서 유의한 증가를 보였다. 이러한 결과는 건강한 성인에서 키네시오 테이핑을 적용한 경우 키네시오 테이핑을 적용하지 않고 트레드밀 운동을 한 경우보다 폐활량의 향상에 효과적이라고 생각되어진다. The purpose of this study was to investigate the variation of vital capacity(VC) according to the effects of kinesio taping with treadmill exercise. Twenty-six non-smokers were participated in this research and these subjects were randomly assigned into two groups. To measure the VC variation effects of kinesio taping, spirometer was used. The collected data were analyzed statistically by using a paired Mauchly test and repeated measure ANOVA. The results of this study were as follows; A group: treadmill with kinesio taping B group: treadmill without kinesio taping. Between A and B, there were significant differences. In the case of A group, there was 11.66% increase of VC, during 3 weeks experiment(p<.01). In the case of B group, there were significant differences, 3.35% increase of VC, during 3 weeks taping intervention(p<.01). After 6 weeks experiment, the improvement of VC shown a significant difference with intergroup (p<.05). From this result, it was revealed that treadmill exercise with kinesio taping was effective to improve VC to healthy adult than treadmill exercise without kinesio taping.

건강보험 청구자료를 활용한 응급의료 이용현황

조수진,최윤정,이재호 건강보험심사평가원 2022 HIRA RESEARCH Vol.2 No.1

부분 무치악 환자에서 마그네슘 이온주입 임플란트의 성공률에 대한 전향적 임상연구

최수정,유정호,이규복,김진욱 大韓齒科補綴學會 2012 대한치과보철학회지 Vol.50 No.3

Purpose: This study examined the clinical success rate of Mg titanate implants (M Implant system, Shinhung, Korea), which employ a Mg coating method, by evaluating the marginal bone loss and implant stability using radiographs and Osstell, over a 1 year. Materials and methods: The locations of the implants placement were divided into 4 areas; the maxillary and mandibular premolars and molars. In the maxilla, 8 and 9 implants were inserted in the premolar and molar areas, respectively. In the mandible, 11 and 51 implants were inserted in the premolar and molar areas. Marginal bone loss and ISQ of all implants (79) were measured after insertion, mounting the prosthetic appliance, and 1, 3, 6, and 12 months after loading. The marginal bone loss was measured from the radiograph using XCP bite, which was customized, and the implant stability measured using Osstell. Fisher's exact test (α=.05) was used to compare the success rates of each region. Results: The mean marginal bone loss for the upper and lower jaws were 1.537 mm and 1.172 mm. The mobility showed a non-significant reduction or increase according with time. The success rates were accounted for 94.12% and 98.39% in the upper and lower jaws; the premolars and molars were accounted for 100% and 96.67%. The two cases of early failure resulted from failure of primary stability during implant insertion. The late failures were not observed for 1 year after adding a loading to the implants. Conclusion: The Mg titanate implant showed good primary stability and good clinical results in both healing and function. 연구 목적: 부분 무치악 환자에서 Mg titanate implant (M Implant system, Shinhung, Korea)의 보철후 1년간의 방사선사진을 이용한 변연골 흡수량과 Osstell을 이용한 임플란트 안정성 평가 결과를 분석하여 임상적인 성공률에 대해 알아보고자 한다. 연구 재료 및 방법: 38명의 환자에 79개의 임플란트를 식립하여 보철 후 1년 동안 변연골 흡수량, 임플란트의 동요도, 임플란트 식립 위치에 따른 분포 및 성공률, 보철 전후에 따른 성공률을 분석했다. 결과: 변연골의 흡수량은 보철물 장착 후 1년간의 평가에서 상악에서는 평균 1.537 mm, 하악에서는 평균 1.172 mm의 변연골 흡수가 관찰되었으며, 전체적인 상하악의 평균 변연골 흡수량은 1.255 mm로 관찰되었다. 수술 후 시간이 경과함에 따라 ISQ값은 미약하게 감소하였다가 증가하는 경향을 보였으나, 상악과 하악 모두에서 식립 당일 이후 예비 인상일, 최종 보철물의 장착일과 주기적인 평가 기간 동안 ISQ값의 큰 증가나 감소를 나타내지는 않았다. 성공률은 상악에서의 94.12%였고, 하악에서의 98.39%였다. 결론: 본 실험의 결과로 미루어 Mg titanate implant는 임상적으로 양호한 결과를 보여주었다.

사용자 수술명과 수술분류 code(ICD-9-CM) 일치율 향상에 관한 연구 : 수술실 OCS program 사용 활성화를 통하여

최향하,김미영,김도진,유지원,장정화,박수정,박재성 한국의료QA학회 2005 한국의료질향상학회지 Vol.12 No.1

Background : The necessity of unify and standardize codes used at hospital has been emphasized since OCS (Order Communicating System) was adopted. Therefore, the purpose of this study were to standardize operation code by continuous training of the ICD-9-CM code that is used as standard code in OCS program at operating room. Method : In 400 operation data, operation code entered in OCS program at operating room was compared to operation name recorded in medical record. In addition, a matching rate between input data of operation code by medical record department and computing input data of operation code in 3,710 cases was compared for each department. User operation name and operation code were matched and major diagnosis by operation department and operation name were also matched. Results : User operation name was reflected in operation classification code in detail, and operation code entered on user was registered. Input rate and matching rate of operation code were gradually improved after improvement activity. In particular, a matching rate was high at ophthalmology where operation name is segmented. Plastic surgery and orthopedics with a lot of emergency operation and comprehensive operation name show low input rates. Conclusions : As the medical field makes progress in computerlization, awareness of information exchange and sharing becomes higher. Among codes to classified medical institution, codes related to surgical operation are all different by user of hospital and department. Computerlization and standardization is essential. And when efforts of standardization continue in alliance with individual hospital and institution, initiative of preparing medical policy data at a national level will be accelerated.

의견불일치 상황에서의 화행 실현 양상 연구 : 일본인 한국어 고급 학습자를 대상으로

최수진 이화여자대학교 교육대학원 2005 이화교육논총 Vol.15 No.-

This research examines the similarities and the differences in 「patterns of speech act realization」in disagreement situations between Native Korean Language Speakers(KK), Japanese Learners of Advanced or Highly-Advanced Korean Language(JK) and Native Japanese Language Speakers(JJ) from Interlanguage Pragmatics' viewpoint through comparison analysis. In this regard, Discourse Completion Test(DCT) was used as an investigation method, and the questions were classified into twelve disagreement situations according to social variables such as intimacy, status, official and personal situations. In order to analyze the collected data, the Head Acts were classified as 「criticism, suggestion, request to talk further, acceptance, reason · explanation, avoidance, insistence」, and the Adjuncts were classified as 「use of formal name-titles, gratitude expression, apology expression, expression of desire for acceptance, emphasis on sympathy, addition expression」. In Chapter I of this paper, the need and the objective of this study are introduced as well as various preceding research of the Korean language education field including past disagreement speech act studies. Chapter ll explores speech act theories and Interlanguage Pragmatics which constitute the logical foundation of this thesis, and reconstructs disagreement speech act strategies and social variables necessary for the concept of disagreement and for analyzing experiment results by modifying to suit this investigation. Chapter III describes investigation tools, creation procedures, data analysis methods and examination tools. In addition, this experiment was carried out after two preliminary experiments. In Chapter Ⅳ, analysis for each group according to twelve situational strategy analysis and three social variables, intimacy, status, official and personal situations were carried out, after analyzing comprehensive strategies for each group regarding the results gained through this experiment. As a result, the order of frequency in use of the Head Acts were 「insistence-reason · explanation-suggestion-criticism」 while request to talk further, acceptance and avoidance strategies were hardly used at all. Performing analysis by social variables, the three groups all used the following order of strategies in disagreement situations with highly intimate people: 「insistence-reasonㆍexplanation-suggestion」. In the case of disagreement situations with a person with higher status, the order 「reasonㆍ explanation-insistence-suggestion」 as used, while 「insistence-suggestion-reason · explanation」 was used for a person of lower status. In official situations, the order of the strategies is appeared to be 「insistence-suggestion-reasonㆍexplanation」 Thus, for all three groups, there seemed to be no difference in strategy order in disagreement situations with highly intimate people, people with higher/lower status, or in official situations. The difference in speech act realizations according to situations of the three groups was that JJ seemed to show a higher tendency to expect others to follow through active insistence than KK, as seen from the fact that JJ uses more insistence strategies than KK. JK, on the other hand, appears to use strategies resembling those of KK as its understanding of the Korean language and the Korean culture is increased. Although all three groups displayed low levels of Adjuncts usage, KK used addition expression while JK and JJ used apology expression the most In disagreement situations. The difference in the groups according to social variables is that in low intimacy cases, KK explains the reason to others when JK and JJ expect others to follow by using assertion strategies. In personal situations, KK seeks to obtain consent from others through explanation while JK and JJ display high tendencies to expect others to follow by using assertion. The results of this investigation is summarized in Chapter V Looking at the results, in low intimacy cases and personal situations, JK uses different strategies than those of KK and thus fails to properly deliver personal feelings causing possible misunderstandings. Therefore, it seems necessary for Korean pragmatics education for JK to use more expressions that are used by KK which will enable JK to respect others and deliver personal opinions correctly at the same time. The results of speech act comparison analysis in disagreement situations for the three groups suggests new ideas for Korean education fields aiming for attaining communication skills. Disagreement occurs in various situations and may damage the face of others, so this study may play a significant role as primary data source for Korean Speech Act education. With continuous in-depth studies of Interlanguage speech act between languages which are similar to Korean culture considering various social variables, it will contribute to more effective Korean teaching-teaming and textbook development.

自然科 實驗學習指導方法의 硏究 : 탐구학습의 효율화를 위한 재료개발

최수안,오진태,옥치율 부산교육대학교 과학교육연구소 1985 科學敎育硏究 Vol.10 No.-

It may be said that the mainpost of natural science study is the inquring study and as for the point of observation and experiment which is a kind of inquiring process, the success of natural science study is dependent upon study materials. Therefore, in order to develop more effective inquring activities, successive studies such as the ploblem solution, improvement and development of study materials are needed. Several units is the field of chemistry and biology are selected, and several study materials improved and developed are examined as followers: 1. Several experiment methods for the game in order to observe the presence of air are tried. 2. In order to observe the solution phenomena of air easily, new experiment designe is devised, ammonia is soluble in the water including two drops of phenolphthalein and the solution phenomena of air changing into red. 3. In the experiment of the generation of carbon dioxide using every kind of shelfish instead of lime, the amount of the generation of carbon dioxide from the concentration of hydrochloric acid and the amount of shelfish is compared and the effort of substitute materials is examined. 4. The utility factor or the inquring study is devised through the utilization of waste materials, the development of substitute materials and the productive activities of instrument observation using study materials used separated units of materials. 5. It is found that a tadpole, a mud-snail and a killifish substitute for a planaria with a living thing material in the field of biology is available for the revival experiment.

조혈모세포이식 환자에서 침습성 진균 감염에 대한 이트라코나졸 액과 플루코나졸 시럽의 예방 효과 : 전향적, 무작위, 비교 임상시험

최수미,이동건,최정현,박선희,엄기성,김유진,김희제,민창기,유진홍,김동욱,이종욱,민우성,신완식,김춘추 대한감염학회 2005 감염과 화학요법 Vol.37 No.2

목적 : 조혈모세포이식 환자에서 호중구 감소 기간 동안 이트라코나졸 액(ITZS)과 플루코나졸 시럽(FCZS)의 진균 감염에 대한 예방 효과를 전향적으로 비교해 보고자 하였다. 재료 및 방법 : 2001년 8월부터 2002년 6월까지 가톨릭 조혈모세포이식센터에 입원하여 동종 조혈모세포이식을 받은 만 18세 이상의 환자들을 각각 ITZS와 FCZS군으로 무작위 배정하여 침습성 진균 감염 발생과 약제 관련 부작용을 전향적으로 조사하였다. 결과 : ITZS군에 40명, FCZS군에 38명이 배정되었고, FCZS군 환자의 평균 나이가 더 많았다. 시험 약제 평균투여기간은 ITZS군 16.4일, FCZS군 21.9일로 ITZS군이 더 짧았다. 이는 ITZS군에서 소화기 부작용으로 인한 약제 중단이 더 많았기 때문으로 보인다. 시험 약제 투여 후 총 빌리루빈 수치의 유의한 증가가 관찰되었으나, 약제 관련 부작용은 모든 환자에서 가역적이었다. 호중구감소 기간동안 표재성 진균 감염은 두 군에서 모두 발생하지 않았고, 침습성 진균 감염은 ITZS군 5명(12.5%), FCZS군 8명(21.1%)으로 FCZS군에서 많았으나, 통계학적으로 유의하지 않았고, 사망률에도 유의한 차이는 없었다. 결론 : 침습성 진균 감염에 대한 이트라코나졸 액과플루코라졸 시럽의 예방 효과에 유의한 차이는 없었으나, 앞으로 더 많은 환자를 대상으로 잘 짝지어진 대조군 연구가 필요할 것으로 보인다. 부작용 중 간독성과 관련하여 이식시 투여되는 여러 약제들과의 상호작용에 대한 연구도 필요할 것이다. Background : Though fluconazole is widely used for antifungal prophylaxis, it is ineffective against mould infections including Aspergillus species. Itraconazole has a broader spectrum than fluconazole but the capsule form shows erratic bioavailability in neutropenic patients. In this study, we compared itraconazole oral solution (ITZS) with fluconazole syrup (FCZS) for the prevention of invasive fungal infection (IFI) in allogeneic hematopoietic stem cell transplant recipients. Materials and Methods : Adults receiving allogeneic hematopoietic stem cell transplantation (HSCT) from september 2001 to June 2002, were randomly allocated to either the ITZS group or the FCZS group. We prospectively evaluated the safety and efficacy of each drug. Results : Out of 78 patients (40 patients in the ITZS group and 38 patients in the FCZS group) who were eligible for this study, 37 patients completed the course of prophylaxis without any evidence of IFI. The mean duration of prophylaxis was 16.4 days for the ITZS group and 21.9 days for the FCZS group (P<0.006). Drug-related adverse events occurred in 28 patients (70.0%) and 19 patients (50.0%) in the ITZS group and the FCZS group, respectively. Common adverse events of ITZS were nausea, vomiting, and diarrhea. Drug-related reversible hepatotoxicity occurred in 4 patients in the ITZS group. There was a significant elevation of total bilirubin level in the ITZS group. The incidence of suspected IFI occurred in 5 patients (12.5%) who received ITZS, compared with 8 (21.1%) who received FCZS (P=0.372). There were no proven IFIs or superficial (oral/vaginal) fungal infections in both groups. Overall mortality was not different between the two groups (2.5% in the ITZS group versus 5.3% in the FCZS group, P=0.610). Conclusion : ITZS and FCZS showed similar protection against IFI during pre-engraftment period. Poor tolerability due to gastrointestinal troubles of ITZS might limit its success as prophylactic therapy. Well matched controlled study with large number of patients will be required in the future.

조혈모세포이식 환자에서 발생한 Cytomegalovirus 질환의 특징 : 일개 대학변원에서 최근 10년간의 경험

최수미,이동건,박선희,김시현,김유진,민창기,김희제,이석,최정현,유진홍,김동욱,이종욱,민우성,신완식,김춘추 대한감염학회 2009 감염과 화학요법 Vol.41 No.1

Background : Studies on cytomegalovirus (CMV) diseases in Korean hematopoietic stem cell transplant (HSCT) recipients are lacking and do not reflect the recent trends of advances and changes. Therefore, we tried to analyze the clinical features of CMV diseases in HSCT recipients over the past 10 years at a tertiary university hospital in Korea. Methods : Retrospective review of medical records was done for all adult HSCT patients who received transplant at the Catholic HSCT Center from January 1998 to January 2008. Results : Forty-four cases (2.2%) of CMV diseases were identified. CMV pneumonia was diagnosed in 17 patients, retinitis in 16 patients, enterocolitis in 7 patients, esophagitis 1 patient, gastritis in 1 patient, duodenitis in 1 patient, and hepatitis in 1 patient. The median onset of symptom was 90 days after transplantation. Late CMV diseases accounted for 47.7%. CMV related death varied from 0 to 58.8% according to the involved organ. CMV retinitis was diagnosed relatively later in the course of transplantation mostly in patients who had chronic graft versus host disease (GVHD). On the contrary, CMV enterocolitis mainly occurred in patients who suffered from acute GVHD. The overall concurrent CMV reactivation was documented to be 63.6%: the concurrent CMV reactivation was observed only in 37.5% of patients with retinitis. Conclusions : We observed some differences in the pattern of CMV disease manifestation according to the involved organ and reconfirmed the fact that CMV pneumonia is the most common and fatal disease in HSCT recipients. Additionally, CMV retinitis was not uncommon in HSCT recipients. Since specific marker does not exist in predicting retinitis, regular ocular examination should be done thoroughly, especially in patients with chronic GVHD.