운동과 중탄산나트륨 투여가 세포막 투과성 변화에 미치는 영향

양정수,최용어,김학렬,이규성,오세진 韓國體育大學校附屬 體育科學硏究所 1992 韓國體育大學校附屬 體育科學硏究所論文集 Vol.11 No.1

본 연구에서는 근피로현상을 규명하기 위한 요인으로서 세포막 투과성의 변화를 평가하는것 이었으며,중탄산나트륨 투여가 세포막 투과성의변화에 미치는 영향을 규명하는것이었다. 신체건강한 성인남자 8명이 연구의 피험자로 동원 되었으며 이들은 400m 트랙에서 1500m 최대런닝을 수행하였으며 이후 중탄산나트륨 300mg/kg.wt의 섭취후에 동일한 실험을 반복하여 수행하였다. 중탄산나트륨 투여전 1500m 최대런닝에 따라 Na??(P<0.01) Ca????(P<0.05)은 크게 증가된 수준을 나타내었으나 K??, Glucose, LDH 및 Pi농도는 증가된 수준을 나타내었으나 유의한차(P<0.05)는 없는 것으로 나타났다. 또한 중탄산나트륨 투여후 1500m 최대런닝에 따른 Na??(P<0.0001)과 Pi(P<0.0001) 농도는 크게 증가되었으나 그 밖의 변인에서는 증가된 수준을 유지하였으나 유의한 차 (P<0.05)는 없는것으로 나타났다. 또한 중탄산나트륨 투여 전, 후간 차를 비교하여 보았을때 1500m 런닝직후 Ca????(P< 0.01)에서만 유의한 차를 나타내었으며, 그 밖의 어떠한 변인에서도 유의한차(P<0.05)는 없는 것으로 나타났다. 결론적으로 1500m 최대런닝에 따라 세포막 투과성의 변화는 피로에 기여하는 중요한 요인이 된다는 것을 평가할 수 있었으며, 1500m 최대런닝으로 유발된 세포막 투과성의 변화는 중탄산나트륨 투여에 의해서는 유의한 영향을 미치지 못한다는 결론을 내릴 수 있었다. The purpose of this paper was to estimate the changes of cell membrane permeability as indicator of muscle fatigue, and the effect of sodium bicarbonate ingestion. Eight male was participated as subject of this study. Subjects were performed a 1500m maximal running as control, Also as sodium bicarbonate ingestion(300mg/kg. wt). In the experiment of control, Na?? (p<0.01) and Ca???? (p<0.05) was shown significant differnce follows 1500m running, but was not shown significant difference(p)0.05) in the K??, Glucose, LDH and Pi concentration. In the experiment of NaHCO?? ingestion, Na (p<0.0001) and Pi(p<0.0001) concentration was significantly increased levels, but was not displyed a significant difference(p >0.05) in other factors. Also, only Ca????, Compared before with after of NaHCO?? ingestion, after 1500m maximal running was shown a significant difference(p <0.01), but was not shown a significant difference(p>0.05) in other factors. We concluded that, The changes of cell membrane permeability followed 1500m maximal running estimated as important factors to induce a muscle fatigue. However, the changes of cell membrane permeability was not proved the improved effect as sodium bicarbonate ingestion.

포도상구균의 약제 내성 및 β-lactamase의 생산에 관하여

양재윤,박상욱,이용진,조규봉,이연태 단국대학교 신소재기술연구소 1994 신소재 Vol.4 No.-

항균제의 오남용으로 내성균의 출혈빈도가 매우 높아져 임상가에서는 환자치료에 어려움이 적지 않다. 특히 많은 균종중에서 병원감염의 문제가 되고 있고 포도상구균에 대한 내성빈도는 환자치료에 중요하다고 판단된다. 따라서, 1992년 12월부터 1993년 2월 사이에 서울대 병원에 내원(來院)한 환자로부터 총 108주의 포도상구균을 분양 받아 항균제 내성검사를 실시하였고 황색포도상구균의 경우는 β-lactamase 생산유무 확인 및 methicillin에 대한 MIC 농도를 비교하였으며, methicillin에 대하여 각기 다른 MIC농도를 갖는 균종에 대한 substrate 분해능 검사를 실시하여 다음과 같은 결론을 얻었다 (1). 항균제 내성검사를 실시한 108주의 포도상구균은 penicillin에 가장 높은 내성을 나타냈다. 황색포도상 구균의 경우는 97%, 표피포도상 구균의 경우 100%, coagulase 음성포도상구균의 경우 95.7%로 나타났다 (2). vancomycin의 경우는 전 균주가 감수성을 나타냈다 (3). methicillin에 대한 내성율은 41.4%로 나타났다. 이 내성균주들은 10가지 검사 항생제중 최고 9가지에 대한 항균제 내성을 나타내어 다약제 내성임을 알 수 있었다 (4). β-lactamase 생산 실험에서는 58균주의 황색포도상 구균중 53주가 양성을 나타내어 91.4%의 결과를 얻었다. β-lactamase 생산균주와 methicillin에 대한 내성균주가 반드시 일치하지는 않았다 (5). Spectrophotometric assay에서는 내성이 높은 균주와 낮은 균주간에 β-lactamase에 의한 substrate 분해능이 서로 유사하게 나와 황색포도상 구균의 methicililin에 대한 내성 획득은 β-lactamase 뿐만이 아닌 penicillin binding protein(PBP)의 구조적 변화로 인한 내성획득도 시사함을 알 수 있다. One hundred and eight Staphylococcus aureus strains were isolated from patients of Seoul National University Hospital from December 1992 to Feburary 1993. All strains were tested for their sensitivities against antibiotics. β-lactamse producing test was also performed in order to know the relationship between the methicillin resistance and β-lactamase production. Its activity was determined by direct spectrophotometric method. Initial velocities of hydrolysis of the antibiotics were monitored at wavelength 323 nm which corresponded to maximal change in absorbance between the unhydrolyzed substrate and the hydrolyzed product. The results are summarized as follows: 1. In antibiotics sensitivity test of 108 isolated strains of Staphylococcus, they showed hightest resistance to penicillin but all of tested strains were susceptible to vancomycin. 2. 41.4% of them showed resistance to methicillin. 3. All methicillin resistant strains were found to be multi-drug resistant, from 3 to 9 antibiotics. 4. 91.4%(53 out of 58) produced β-lactamase, β-lactamase producing strains did not always coincide with the methicillin resistant strains. 5. β-lactamase activities were similar among staphylococus strains, irrespective of their methicillin resistance.

효소 전처리 BCTMP 시트의 노화 거동

양봉숙,김형진,강규영,조병묵,오정수 동국대학교 생명자원과학대학 연습림 2000 연습림논문집 Vol.- No.6

It was generally known that the aging treatment of paper is accompanied with the reductions in physical and optical properties of individual fibers and sheet. In particular, the paper prepared with mechanical pulps occurs the yellowing of sheet, during aging, due to the absorption of light or temperature, and finally has limitation in widely applicable functions of paper. The aging behaviors of paper can be discussed with three groups of factor. One is considered to external factors, like heat, light and moisture. Another is commented with environmental factors, like oxygen, nitrogen, sulfur, and other air pollutant materials. The other is explained by internal factors, like pulp types, lignin contents, and so on. In this paper, the aging behaviors of paper prepared with BCTMP were studied in order to know how to affect in physical and optical properties of paper. For modification of fibers properties, a commercial enzyme was pretreated in the slurry of BCTMP, before handsheet-making. Sheets were aged by accelerated heat treatment. The comparisons were carried out in mechanical strength properties and color reversions. Aging treatments were resulted in higher brightness reductions, in enzyme pretreated paper.

한강변 응급센터에 내원한 익수환자에 대한 임상적 고찰

양진홍,박규남,최승필,김영민,김세경 대한응급의학회 2001 대한응급의학회지 Vol.12 No.2

Background: There are approximately 2,400 drowning deaths reported every year in Korea, but there are few good reports about submerged patients visiting the emergency room, so this study was designed to investigate the clinical characteristics and outcomes of submerged patients treated at an emergency medical center near the Han river. Methods: A retrospective review of 123 cases of submerged patients admitted to our emergency medical center over a 5 year period from 1996 to 2000 was conducted. Results: Among the 123 submerged patients, there were 66 males and 57 females, and their mean age was 33.18. The common cause of submersions was a suicide attempt, followed by lack of attention, unknown etiology, and water sports such as swimming. Most of the patients were transported to ER by 119 ambulance. In-hospital CPR was performed on 27(22%) cases. At initial ECG rhythm of these cases, asystole was noted if 25 patients; VF and pulseless electrical activity, respectively, were noted in two others. Prehospital CPR had been performed on all in-hospital CPR cases except 2. Prolonged ROSC after succesful CPR was noted in 13 cases, The mean duration of CPR was 26 minutes. Five patients survived after CPR. Their neurologic outcomes were assessed on CPC(cerebral performance category). Three patients were categorized in CPC1, the other two patients were categorized in CPC3 and CPC4. Conclusion: Submerged patient's visits to emergency medical center near the Han river were most frequent in summer for suicide purposes. Drowned patients were more hypothermic than other cardiac arrest cases, and resulted in better outcome even with prolonged arrest time, if they were given appropriate prehospital CPR and aggreissive in-hospital resuscitation.

Streptomyces의 mutanase 유도에 관한 연구

양규호,정진 大韓小兒齒科學會 1996 大韓小兒齒科學會誌 Vol.23 No.3

The mutan containing α-1,3 bond is an insoluble portion of glucan which is the main component of dental plaque. The secretion of mutanase was assessed with mutan-digesting Streptomyces isolated from soil, and the factors affecting its activity was studied, obtaining the following result. Mutan-digesting Streptomyces was identified as Streptomyces exfoliatus by its characteristics. The effect of dextranase was identified on the media containing blue dextran. A clear zone was produced by Streptomyces exfoliatus on the media containing blue mutan, so showing the secretion of mutanase. A clear zone was significantly produced on the media overlayed with agar containing blue mutan. A clear zone was produced at 2 days after the inoculation of Streptomyces exfoliatus on the media containing below a concentration of 0.025% glucose, at 3 days on the media containing 0.05% glucose, and at 4 days on the media containing 0.1% glucose. Mutan-digestion wasn't appeared early by adding other carbohydrates. The higher concentration of peptone, the later appearance of clear zone was on the media containing below a concentration of 0.1% peptone. These results indicated that the secretion of mutanase was identified from mutan-digesting Streptomyces on the media containing blue mutan, and a clear zone was appeared lately on the media containing higher amount of glucose.

Self etching primer를 사용하여 부착된 교정용 브라켓의 전단결합강도의 비교

양진영,김민지,임용규,이동렬 대한치과교정학회 2007 대한치과교정학회지 Vol.37 No.4

이면성 및 도플러 심초음파도를 이용하여 진단한 선천성 거대 좌회선지-좌심실 관동맥루

진승원,박준철,이영중,유병현,박현옥,김용주,윤호중,김재형,홍순조,최규보,권종범,원용순,박건 대한순환기학회 2000 Korean Circulation Journal Vol.30 No.4

프로스타글란딘 E₁에칠에스테르의 외용 리오겔 제제 설계

양성운,이진교,이지은,김희규,박혜숙,김종석,최한곤,용철순,최영욱 한국약제학회 2004 Journal of Pharmaceutical Investigation Vol.34 No.2

External lyogels containing prostaglandin E₁ethyl ester(PGE₁-EE), a produrg of prostaglandin E₁(PGE₁) as a therapeutic agent for erectile dysfunction, were formulated to overcome the aqueous instability and enhance the percutaneous absorption. Lyogels of PGE₁-EE were prepared with ethanol (EtOH)/proplyene glycol (PG) cosolvent system as a vehicle, cineol as an enhancer, and hydroxypropylcellusose as a gelling agent. In vitro percutaneous absorption studies were performed to determine the rate of PGE₁ absorption through rat or hairless mouse skin. The permeability of PGE₁-EE lyogel with enhancer was 16-fold greater than that of lyogel without enhancer. Cosolvent produced 9-fold increase in percutaneous absorption. Pharmacodynamic effects of lyogels were evaluated in mature male cats in terms of intracavernosal pressure (ICP). Lyogles containing 0.01% of PGE₁-EE showed higher ICP compared to intraurethral preparation of PGE₁(1%) and enhancer-free control lyogel. The shelf-life (t_(10%) of lyogel at refrigerated condition (4℃) was calculated as 928 days, which is 4.2 times longer than that of control hydrogel. As a result, PGE₁-EE was formulated successfully to a lyogel system with a selective enhancer and cosolvent system for the topical delivery of PGE₁.

가시오가피(Acanthopanax senticosus)의 석유에테르 추출물 중 polyacetylene계 물질의 동정

양효진,김은미,장규섭 충남대학교 농업과학연구소 2008 농업과학연구 Vol.35 No.1

This study was conducted to isolate polyacetylenes from the Acanthopanax senticosus and to identify the chemical structure of the polyacetylenes by UV, IR, ¹H-NMR and (13)^C-NMR. One of the liposoluble materials was extracted with petroleum ether. Polyacetylene compounds were collected through solvent fractionation at silica gel column chromatograph. The HPLC was used for the semi-preparative separation IR spectra of fraction 5 showed triple bonds at 2256 cm^(-1) and double bond at 1654cm^(-1), respectively, ¹H-NMR spectra of Fraction 5 showed the double bond at 5.35-5.48 ppm. Triple bond at 64.0. 71.2, 74.2, 80.2 ppm and double bond at 121.89, 133.0 ppm were observed in the (13)^C-NMR spectra. 가시오가피를 실온에서 petroleum ether로 추출한 polyacetylene의 동정하기 위하여 TLC, HPLC, UV spectrum, IR, NMR로 수행하였다. TLC에 전개시킨 결과, polyacetylene 표준품과 동일한 band가 fraction 5에서 확인되었으며, HPLC에서 fraction 5를 분리시킨 결과 retention time이 4.40, 5.36, 6.40분이었다. 이를 UV spectrum에서 확인한 결과, 6.40분의 peak(compound 3)에서 polyacetylenes의 파장인 231.0nm, 239.0nm, 257.0nm을 나타내었다. IR spetrum에서 triple bond 2256cm^(-1)과 double bond1654cm^(-1)의 전형적인 peak를 나타내었으며, (13)^C-NMR(400MHz, CDC1₃)에서 polyacetylenes 전형적인 64.0, 71.2, 74.2, 80.2ppm은 2개의 triple bond에 의한 peak와 121.89, 133.0ppm에서 internal double bond로 결합된 2개의 peak를 확인 할 수 있었다.

백서의 복강내에 투여한 Mitomycin-C-Polyglycolic acid 제형이 장기에 미치는 영향에 관한 연구

송규상,강대영,최정목,노승무,정경수,오정연,김진향,양준목,최선웅,이진호,조준식,민병무,김용백,김창식,박근성,김승영,김학용,인현빈 충남대학교 의과대학 지역사회의학연구소 1998 충남의대잡지 Vol.25 No.1

To evaluate the in-vivo effects of mitomycin-C-polyglycolic acid composite(MMC-PGA) the disk: shaped composite were implanted into the peritoneal cavity of the Sprague-Dawley rats. The pathologic changes were examined at various time points up to 12 weeks. Initially the soft tissue around the inserted disks showed capillary congestion at 3 days. Inflammatory infiltrates with foreign body giant cells appeared from the 2nd week and reached peak response at 6-8 weeks. These reaction diminished prominently at 12 weeks. No specific pathologic change was found in the liver, the kidneys, and the heart. The above results suggest that the MMC-PGA composite can serve as a new device for intraperitoneal chemotherapy of various types of cancers.