수입각증후군에 의한 급성 복증 1례

정은욱,지삼룡,이영태,박지훈,김동기,제인수,채두근,박성재,박은택,이연재,이상혁,설상영,정정명 白中央醫療院 2005 仁濟醫學 Vol.26 No.1

Afferent loop syndrome is an uncommon complication of a gastrectomy and Billroth Ⅱ reconstruction. It may cause symtoms at any time from the first postoperative day to many years after the gastrectomy. Afferent loop syndrome is characterized by abdominal pain, vomiting and elevation of serum amylase. Thus, it is difficult to differentiate afferent loop syndrome from other cause of acute pancreatitis. However, the history of gastrectomy can be an important clue for diagnosing afferent loop syndrome. We experienced one case of chronic afferent loop syndrome with acute pancreatitis. After appropriate management, the abdominal pain disappeared and serum amylase level decreased. We report this case with a review of relevant literatures.

갑상선 유두암이 병발된 가족성 대장 용종증 1예

백종현,소창배,이은영,이지숙,신은경,박은호,서정아,양재홍,송준영,박선자,박요한,최영식 고신대학교의과대학 2007 고신대학교 의과대학 학술지 Vol.22 No.2

Familial adenomatous polyposis (FAP) is an autosomal dominant syndrome, typically characterized by multiple colorectal adenomas and increased incidence of colorectal carcinomas if it is not treated. Moreover, a variety of extracolonic manifestation are seen. The prevalence of thyroid tumors developing in patients with FAP is about 1∼2%. Generally, papillary thyroid cancer indicates low mortality but ,however, as people get older, the cancer becomes more aggressive. So, the operation is required. Recently we experienced a case of FAP, presenting with papillary thyroid carcinoma, and reported with a brief review of literatures.

체형을 고려한 맞춤형 넥타이 개발에 관한 연구(제1보) : 넥타이 길이를 중심으로 Focused on the Length of Necktie

박은경,홍지원 한국의류학회 2003 한국의류학회지 Vol.27 No.11

The purpose of this study was to develop customized necktie design according to Korean adult male's body characteristics. For this purpose, experimentations of visual sensory evaluation on visual stimuli which show a man's wearing various neckties were carried out. The subjects were students(n=130) majoring in fashion and design related area and experts(n=6). The data were analyzed by ANOVA, duncan test, pearson's correlation coefficient, cross tabulation using spss pc^(+) program. Also statistic of Korean adult men's body measurements(1997) were analyzed and used to decide appropriate necktie length according to the customer's various height. The results were as follows: First, there were significant difference in visual sensibility on the stimuli showing 1) relative location of belt and necktie. 2) neckties with various length using golden ratio to the experimental model's height. Therefore, the need of producing neckties with different length according to customer's various height were verified. Second, neckties with three different length(140㎝, 145㎝, 151㎝) were suggested for the groups of 160㎝ height, 170㎝ height, 180㎝ height according to Korean adult male's body characteristics and aspect of production.

UV/H2O2 광산화공정을 적용한 원전 발생 방사성 폐액내 세제 처리

박은정,이지훈,박세문 대구대학교 환경기술연구소 2004 미래환경기술 Vol.1 No.1

원전 발생 방사성 폐액 내 세제를 제거하기 위한 전처리 공정으로 UV 광산화 공정을 활용하기 위하여 공정의 효율 및 적용 가능성에 대한 기초 실험을 수행하였다. 최적 처리조건을 도출하고자, 방사성 폐액의 평균 세제 함유량 (TOC 50∼100ppm)을 기준으로 모의폐액을 제조하여 과산화수소 주입량에 따른 세제 제거율, pH 변화에 따른 세제 제거율, 공기 공급량에 따른 세제 제거율을 도출하였다. 실험결과로서 과산화수소 주입량은 1000ppm, pH는 9.5일 때 최적 분해율을 보였고 공기 공급량의 영향은 받지 않는 것으로 밝혀졌다. 광분해 특성 규명을 위한 회분식 광분해 실험 결과, 광분해 반응 차수는 2차 반응임을 알 수 있었다. To apply the UV-photo oxidation process for the removal of detergent contained in the radwaste generated from the NPP, the effectiveness and feasibility were studied through the fundamental experiments. The simulants with TOC 50∼100ppm made of detergent were used for the lab-test. The optimum detergent removal rates due to the hydrogen peroxide volume, pH and air injection volume were investigated. The detergent removal rate was optimum at 1000ppm of hydrogen peroxide volume and at pH 9.5, while the air injection volume was not that much effective. The experimental result showed that the photo-oxidation degradation under the batch mode was of the second reaction.

부신피질 기능저하증에 대한 치료 1례

김지은,박형진,송근호 忠南大學校 獸醫科大學 附設 動物醫科學硏究所 2011 動物醫科學硏究誌 Vol.18 No.1

A 11-year-old castrated mongrel dog with the hypoadrenocorticism was referred to the Veterinary Medical Teaching Hospital of Chungnam National University. Hypoadrenocorticism was diagnosed by the clinical signs, the physical examination, laboratory examinations(complete blood count, serum chemistry), ACTH stimulation test and radiography. Clinical signs were improved after fludrocortisone treatment on the basis of ACTH stiluation test.

기체크로마토그래피/질량분석계에 의한 물시료 중 EDTA와 NTA의 분석 및 위해성 평가

박송자,표희수,홍지은 한국환경독성학회 2000 환경독성보건학회지 Vol.15 No.3

Ethylenediaminetetraacetic acid (EDTA) and nitrilotriacetic acid (NTA) are various applied as chelating agents for metal ions, then they are widely used in many industrial processes and domestic products. A method is described for the determination of EDTA and NTA in water samples by GC/MS. The reaction temperature, reaction time and pH for esterification of EDTA and NTA were investigated using 10% sulfuric acid-methanol, ethanol and propanol. Optimum conditions were obtained by the esterification in 80℃ for 1hr with ethanol. Method detection limits of ethylated EDTA and NTA in the 200 ml of water samples were 0.05ng/ml, respectively. EDTA and NTA could be determined in the range of 0.05~23.6 and 0.05~7.0ng/ml in treated water, and in the range of 0.06~25.0 and 0.05--6.40ng/ml in raw water respectively. Risk assessments with EDTA and NTA exposure by drinking water ingestion were carried out, Based on the results of analysis, chronic daily intakes of EDTA and NTA would be less than the value of acceptable daily intake or tolerable daily intake.

조혈모세포로 형질도입에 있어서 Vesicular Stomatitis G Envelope의 효과

박지윤,유은선 梨花女子大學校 醫科大學 醫科學硏究所 2002 EMJ (Ewha medical journal) Vol.25 No.1

목적 : 조혈모세포에서 viral vector를 이용한 안정된 유전자 전달은 유전성 혈액질환의 유전자 치료의 실현에 우선되어야 한다. 그러나 실제로 조혈모세포의 생물학적 특성 등으로 인해 유전자 도입율이 현저히 낮아 임상적인 적용에는 제한이 있다. 저자들은 조혈모세포로 VSV-G 외막을 사용한 HIV vector의 형질도입 효과를 알아보고자 하였다. 방법 : VSV-G를 이용한 HIV-1 vector를 calcium phosphate coprecipitation 방법으로 293T 세포에 형질도입시켜 재조합 농축 바이러스를 얻었다. 분리한 CD34+세포에 재조합 농축 바이러스(10배 농축)를 넣은 다음 4㎍/㎖ polybrene(Sigma)를 첨가하였다. 각 well당 RPMI로 총 500㎕로 맞춘 다음 37℃, 5% CO_2 환경에서 배양하면서 24시간, 48시간 후에 형질도입의 효과를 유세포기와 면역형광 현미경으로 분석하였다. 결과 : HIV 재조합 바이러스가 형질도입된 CD34+ 세포는 YFP의 발현으로 초록색의 형광을 나타내었다. 유세포 분석상 CD34+ 세포로 형질도입율은 배양 24시간 후에는 4.7±2.4%였고, 48시간 후에는 5.7±2.7%였다. VSVG를 사용하지 않은 경우에서는 0.23±0.33%로 저조하였다. 결론 : VSV-G 외막에 의한 HIV vector를 사용하여 CD34+ 세포로 형질도입이 효과적임을 증명하였다. 이런 결과는 조혈모세포를 이용한 유전자 치료의 확대에 기여할 수 있을 것이다. Objectives : Stable gene transfer to human pluripotent hematopoietic stem cells is an attractive strategy for the curative treatment of many genetic hematologic disorders. In clinical tral, the level of gene transfer to this cell population have generally been low. In this study we have evaluated the efficiency of gene transfer to human umbilical cord blood(UCB) CD34+cells using vesicular stomatitis virus glycoprotein G(VSV-G) pseudotyped HIV-1 vector. Method : High titers of replication-defective VSV-G pseudotyped HIV-1 based vector encoding the enhanced yellow fluorescent protein were produced by transient transfection. Human CD34+cells purified from UCB were incubated with pseudotyped HIV supernatants for 24-48 hours. The transduction efficiency were measured by marker gene expression under the microscopy and flow cytometry. Results : Transduction rates into CD34+ were low at 0 and 24 hours, reflecting 4.7±2.4% at 24 hours, they were increased to 5.7±2.7% at 48 hours. Conclusion : We demonstrate efficient transduction of purified human UCB CD34+ by HIV vectors pseudotyped with VSV-G. The results extend the lentiviral vector to clinical gene therapy using human pluripotent hematopoietic stem cells.

Alcohol의 투여에 따른 쥐의 장기에서 생성되는 과산화물의 정량에 관한 연구

박동기,정영진,이지은 建國大學校 附設 自然科學硏究所 1992 建國自然科學硏究誌 Vol.3 No.-

Sprague-Dawley rat 16마리를 control group, 25% alcohol-dose group, 40% whisky-dose group의 각각 6마리씩으로 나누어 식이하여 다음과 같은 결과를 얻었다. 혈청에서 지방의 분석을 total lipid, triacylglycerol, total cholesterol의 3가지를 측정한 결과, total lipid와 triacylglycerol은 alcohol-dose group, whisky-dose group 모두 contrlo에 비해 높은 값이 나타난 반면, total chloesterol의 양은 별 차이를 보이지 않았다. 장기의 중량을 측정함에 있어 liver에서만 유의하게(ㅔ0.05) whisky-dose group에서 높았다. TBA에 의한 hydroperoxide 측정은 liver, brain, lung, kidney, tetis brain 경우엔 whisky-dose group에서 증가하였고, lung의 경우에는 오히려 control group에서 높은 값을 보였다. Three groups of Sprague-Dawley rats(control,25% alcohol-dose, 40% whisky-dose) were used in this experiments. In plasma, the amounts of total lipid and triacylglycerol in the alcohol-dose and whisky-dose groups were increased than that of control groups. Relative weight of kidney, spleen testis was not affected by alcohol-dose and whisky-dose groups, but that of liver was significantly(p<0.05) increased in the 40% whisky dose group. The amounts of lipid peroxide in liver, kidney, testis tended to be increased in 25% alcohol dose groups, but not in lung and brain. In lung, the value of malondialdehyde(2nd product of hydroperoxide) of alcohol-dose groups was lowr than control groups, and in brain it was a little more increased in higher concentration of alcohol. The value of hydroperoxide of alcohol groups in the rat liver was significantly(p<0.01) increased compared with the control groups. There was a significant relation between alcohol consumption and lipid oxidation. Alcohol diet to the rat caused increased of lipid content and peroxidation.

건강한 성인에서 발병한 칸디다와 연관된 위궤양 1례

박지훈,이상혁,김희,이재호,박성재,지삼룡,양성연,박은택,이연재,설상영,정정명,강미선 白中央醫療院 2005 仁濟醫學 Vol.26 No.1

Fungus such as Candida albicans is a normal flora that is frequently found in the oral cavity, gastrointestinal tract, genitourinary tract, vaginal mucosa in a normal person. However, candida can cause opportunistic infection in an immune compromised host. Candidiasis has broad spectrum of disease from mucocutaneous infection to invasive or disseminated infection. But, it is rarely reported that candida is associaed with gastrointestinal tract disease in a healthy adult. The case of gastric ulcer associated with candida particularly in a health adult is reported with relevant literature.

피부 적용 겔제제의 첨가제에 의한 수분 보유력 평가

이은미,박종희,경기열,전일순,조혜정,지웅길 충남대학교 약학대학 의약품개발연구소 2004 藥學論文集 Vol.19 No.-

The aim of this study was to improve the moisturizing and water retaining effects of a gel containing poloxamer 407, glycerin, sod hyaluronate, sorbo(sorbitol 70%). Topical preparations were formulated as a gel containing different concentration poloxamer 407, glycerin, sod. hyaluronate, sorbo(sorbito1 70%), lipid(ceramide. sphingosine ratio 1:l). We tested a various combination of different additives for the moisturizing effect The water holding capacity of the mixture was measured using a moisture analyzer. In vivo test was carried out on human skin using Skicon-200 and water retaining effect was increased by addition of lipid. Water evaporation test was performed by various concentration of additives and water evaporation characteristics of the preparations were determined using the moisture analyzer. Moisturizing and water retaining effects was increased by adequate combination of various additives with topical gel formulations.