화기삼에서 분리한 내생세균의 다양성 및 항균활성

홍지은,김장욱,이정우,방경환,조익현 한국약용작물학회 2018 한국약용작물학술대회 발표집 Vol.2018 No.05

Background : Endophytes are generally regarded as beneficial microorganisms that live in plant tissues without disease symptoms. The endophytic species may differ depending on the plant age, the sampled time, the plant genotype, and the tissue. Although numerous endophytes have been identified from various plants, little is known about the endophytic bacteria of Panax quinquefolius, a useful herb plant. Therefore, the aim of this study is to investigate diversity and distribution of endophytic bacteria from 2-years-old to 6-years-old P. quinquefolius and to evaluate antimicrobial activity of isolates. Methods and Results : Initially, 2-years-old to 6-years-old plants were collected and sterilized with 70% ethanol and sodium hypochlorite, subsequently we prepared individual suspensions which were mixed with sample and sterile distilled water. A total of 88 single colonies were obtained from the LB agar plates spreading suspension. Using 16S rDNA sequencing, the taxonomic status of the isolates were determined consequently 42 species were identified. The 42 species were classified into 4 phylum; Proteobacteria (64%), Firmicute (27%), Actinobacteria (8%), and Bacteroidetes (1%). Based on age, the isolates of 5-years-old plant showed highest diversity. Moreover, Actinomycetales, Bacillales, and Pseudomonadales were equally dispersed as predominant orders in 5-years-old plant. The antagonistic activities of isolates against a phytopathogen, Pseudomonas syringae pv. tomato DC3000 : GFP (Pst : GFP) were performed using dual culture assays. We measured antibacterial activity by quantifying fluorescence of Pst:GFP which representing pathogen growth. As a result, 36 isolates inhibited growth of Pst:GFP. Interestingly, all species belonging Pseudomonas in this study showed strong antibacterial activity against Pst : GFP. Conclusion : These results improve our understanding of the structure and diversity of the endophytic bacteria of P. quinquefolius. Furthermore, we suggest that bacterial endophytes with antimicrobial activity might have useful as materials for biocontrol agents.

사회불안성향 여대생의 사회불안 및 자아존중감에 대한 인지행동적 집단미술치료의 효과

홍지은,장석환 한국미술치료학회 2019 美術治療硏究 Vol.26 No.4

The purpose of this study was to examine the effects of group cognitive behavioral art therapy on the social anxiety and self-esteem of female undergraduates with social anxiety tendency. Several female students with difficulties in interpersonal relationships or speech were recruited from a university. Then, group cognitive behavioral art therapy with 10 sessions were conducted, and pre-post test scores were compared to investigate the differences between participants’ social anxiety and self-esteem at both time points. The following quantitative measures were used: Social Avoidance and Distress Scale (SADS) and Speech Anxiety Scale (SAS) for the social anxiety variable, and Self-Esteem Scale (SES) for the self-esteem variable. The results revealed that, after the program, participants experienced (a) reduced social avoidance and distress, (b) decreased speech anxiety, and (c) increased self-esteem significantly. In sum, group cognitive behavioral art therapy in this study reduced the female undergraduate students' social anxiety and enhanced their self-esteem. Lastly, the limitations of this study and possible future studies were discussed. 본 연구는 사회불안 성향이 있는 여대생들의 사회불안과 자아존중감에 대한 인지행동적 집단미술치료의 효과를 알아보고자 하였다. 이를 위해 수도권 소재 한 대학교에서 대인관계나 발표불안에 어려움이 있는 여학생들을 모집하여 총 10회기의 인지행동적 집단미술치료를 실시하여 사전-사후검사를 통해 그 변화를 살펴보았다. 사회불안은 사회적 회피 및 불편감척도(SADS)와 발표불안 척도(SAS)를, 자아존중감은 자아존중감 척도(SES)를 사용하여 양적 분석을 하였다. 그 결과, 첫째, 프로그램 실시 후 여대생의 사회적 회피 및 불편감이 유의미하게 감소하였고, 둘째, 발표불안도 감소하였으며, 셋째, 자아존중감이 증가하였다. 즉, 인지행동적 집단미술치료가 여대생의 사회불안 감소와 자아존중감 향상에 효과가 있었다. 본 연구는 기존의 아동과 청소년을 대상으로 한 연구들과는 달리 대학생을 대상으로 인지행동이론에 기반을 둔 사회불안 집단미술치료를 실시하여 그 효과를 입증한 것에 그 차별성과 의의가 있다. 마지막으로 본 연구의 한계와 가능한 후속연구를 논의하였다.

Isolation of novel leaf-inhabiting endophytic bacteria in Arabidopsis thaliana and their antagonistic effects on phytophathogens

홍지은,조성희,문주연,이정숙,권석윤,박정미 한국식물생명공학회 2015 Plant biotechnology reports Vol.9 No.6

Bacterial endophytes benefit the host with protection against abiotic and biotic stresses and through increased plant growth. In this study, we screened novel endophytes for the ability to endophytically inhabit leaf tissues. Four endophytic bacteria were screened from the apoplastic fluids extracted from the uninfected upper leaves of Arabidopsis thaliana 3 days postinoculation (dpi) with Pseudomonas syringae pv. tomato DC3000 (Pst). Under sterile plant growth conditions, we showed that the four isolates proliferated intercellularly in the leaf tissues of A. thaliana. Based on 16S rRNA sequencing analysis, the identities of the inoculated endophytes and the re-isolates from the leaf tissues were confirmed. Among the isolates, the two species of Rhodococcus were the first members of the genus to be identified as leaf-inhabiting endophytes. Additionally, three of the isolates showed antagonistic activities, with different levels of activity, against Fusarium oxysporum pv. conglutinans (F.o.) and/or Pst. Furthermore, the application of one isolate, Bacillus cereus KB1, on tomato plants significantly increased the disease resistance to Botrytis cinerea and Pst. In combination, these results indicate that these endophytic isolates can be used to develop potential biocontrol agents against a variety of pathogenic fungi and bacteria.

청도소싸움을 통해 본 전통여가의 근대화 과정

홍지은,임용석,김매이 여가문화학회 2015 여가학연구 Vol.13 No.1

Cheongdo bullfighting is a prominent case which presents the modernization of traditional leisure culture in Korea. The primary purpose of this study is to investigate traditional leisure activities’–Cheongdo Bullfightings’– modernization process. The study carefully investigates the different stages during its process of modernization. The modernization process of Cheongdo bullfighting could classified under five heads from 1970 to 2012, 1) reemergence of tradition, 2) modernization emerging, 3) glory days of bullfight 4) investment boom and 5) confusion period. This study examined the modernization of traditional leisure culture and the changes through historical analysis. The study used the periodical information from newspapers, magazines and researches from 1970s. By looking into the commercialization of bullfight and related groups' interests, The purpose of the study rely on providing basic information of managing and over grieving the process of commercialization and merchandising of traditional leisure culture by looking at the changes of Cheongdo bullfight. 본 연구의 목적은 청도 소싸움의 근대화 과정을 면밀히 검토해 봄으로서 전통여가의 근대화 과정을 이해하여 향후 전통여가 관리 및 발전에 도움이 될 수 있는 기초자료를 제공하는데 궁극적인 목적이 있다. 한국 전통여가의 근대화 과정을 분석하기 위해 역사연구방법을 사용하였으며, 전통적 경기방식을 유지하며 현재까지 지속되고 있는 가장 큰 규모의 소싸움 대회인 청도소싸움을 사례로 선정하였다. 본 연구는 문헌조사를 통하여 전통여가의 근대화 과정을 분석하였으며, 현장연구 및 면담을 병행하여 이를 보조 자료로 활용하였다. 분석결과 청도소싸움의 근대화 과정은 1970년부터 2012년을 기준으로 총 5단계의 변화과정을 거쳐 왔다. 1)재현기(1970~1990), 2)도입기(1990~1994), 3)번창기(1995~1998), 4)전성기(1999~2002), 5)호황기 및 혼란기(2003~2012). 변화되는 과정에서 청도소싸움의 의미는 시기별로 다르게 도출되었으며, 이러한 변화는 사회의 흐름과 정부의 개입, 행위 집단의 변화 등과 밀접한 관계를 이루고 있다. 청도소싸움의 ‘전통적 의미’ 또한 변화하는 사회구조 속에서 다양하게 해석되었다. 따라서 본 연구에서는 ‘전통’의 현대적 의미 해석과 현대 전통여가의 절충적 발전방향을 이를 통해 제시하고자 한다.

커먼즈를 둘러싼 정치와 변동하는 도시 커먼즈 : 청주시 산남 두꺼비 생태마을을 중심으로

홍지은 한국환경사회학회 2022 환경사회학연구 ECO Vol.26 No.1

This study analyzed changes in urban commons focusing on Toad Eco Village in Cheongju. The commons management perspective is concerned with deriving a system or rule that maintains the commons within the community. However, the commons are not already given or fixed in a stable state. Therefore, it is necessary to pay attention to the political process in which commons are formed or disbanded based on the theory of commons politics. The commons has both the aspect of common rights and the autonomous aspect of a community with specific boundaries. Also, these two aspects have a conflicting relationship depending on their boundaries. This study confirmed the following results while analyzing changes in urban commons. First, creating urban commons can relieve tension between the two dimensions and boundaries of commons and secure strong power when complementary relationships are formed. Second, the problem of ‘ownership’ must be resolved to create urban commons. Third, the urban commoning is a struggle and resistance that changes the meaning and role of the state and the public. 이 연구는 도시 커먼즈가 변동하는 정치적 과정의 구체적인 양상과 동학을 밝히기위해 ‘청주시 산남 두꺼비 생태마을’을 중심으로 분석하였다. 커먼즈 관리에 중점을둔 논의에서는 주로 커먼즈를 지속하는 공동체 내부의 제도나 규칙을 도출하는 데주요한 관심을 둔다. 그러나 대다수의 공간이 상품화된 현대 자본주의 도시에서 커먼즈는 이미 주어지거나 안정적인 상태로 고정되어 있는 것이 아니다. 따라서 이 연구는 커먼즈 정치에 관한 논의를 토대로 커먼즈가 구성되거나 해체되는 정치적 과정에주목해야 할 필요성을 제기한다. 커먼즈는 일정한 경계를 가지는 공동체의 자치적인차원을 가지는 동시에 집합적이고 보편적 권리의 차원을 가지며, 이 두 차원이 가지는 경계에 따라 일정한 긴장 관계를 내포한다. 이와 같은 커먼즈 경계를 중심으로 도시 커먼즈 변동을 분석하는 가운데 다음과 같은 지점을 확인하였다. 첫째, 도시 커먼즈 만들기는 커먼즈가 지닌 두 차원과 그 경계에 따른 긴장을 완화하고 상호보완적인관계를 맺을 때 커먼즈 구성을 강화하는 힘을 확보할 수 있다. 둘째, 도시 커먼즈 구성을 위해서는 사익에 중심을 두는 ‘소유권’의 문제를 풀어가야 한다. 셋째, 도시 커먼즈만들기는 국가와 공공(public)의 의미와 역할을 변화시키는 투쟁과 저항 속에서 이뤄진다.

다양한 체육학 분야에서 포토보이스 연구방법 적용가능성

홍지은,최재섭 한국사회체육학회 2018 한국사회체육학회지 Vol.0 No.72

The purpose of this study is to introduce the concept and process of photovoice, to review relevant research cases, and to discuss issues in photovoice applications for the sports-related research domain. Different types of photovoice procedures and questions were reviewed. We hope that this study enables other researchers to expand topics by understanding and applying a rather new method in the sports-related research field. To review previous literature, the keyword ‘photovoice’ was first searched and then narrowed into ‘sports’ ‘physical activity’ ‘leisure’ and ‘physical education’ on the database. With increasing emphasis on community-based participatory research, photovoice methods have been diversely used to embrace various groups including vulnerable social groups and minorities. In the field of sports, a number of recent studies have identified the participants’ perspectives and experiences of sport in specific contexts. This study suggests the possibilities of applying the photovoice methods for different expended topics, participants, domains and policies in the sports-related research.

하이드로겔을 이용한 줄기세포의 분화 제어

홍지은,이은지,허지승,황석연 한국생물공학회 2013 BT NEWS Vol.20 No.1

기체크로마토그래프/질량분석계에 의한 물시료 중 1,4- dioxane의 분석 및 위해성 평가

홍지은,표희수,박송자 한국환경독성학회 2003 환경독성보건학회지 Vol.18 No.3

1.4-Dioxane is used as a solvent for lacquers, paints, varnish removers, dye baths and printing compositions. And it is also used for detergent preparations, cosmetics, deodorants and fumigants. A method is described for the determination of 1, 4-dioxane in water samples by GC/MS. The extraction recoveries were studied for some solvents and solvent volume ratio were investigated using t-butyl methyl ether(MTBE). Optimum condition was obtained by the liquid-liquid extraction using the 10mL of MTBE for 10mL of water. Method detection limit of 1,4-dioxane in the 20mL of water samples was 0.05ng/mL. It could be determined in the range of 0.24~240ng/mL in treated water, and in the range of 0.69~81.9ng/mL in raw water, respectively. Risk assessments with 1, 4-dioxane exposure by drinking water ingestion were carried out. Based on the results of analysis, chronic daily intake of 1, 4-dioxane was 2.22×10^(-4)mg/kg/day and excess cancer risk was calculated to be 2.44×10^(-6).

판타지스포츠의 국내 도입을 위한 제도화 가능성 모색: 미국 판타지스포츠 현황분석을 중심으로

홍지은,김민수 한국체육정책학회 2019 한국체육정책학회지 Vol.17 No.2

The purpose of this study was to analyze the institutionalization possibility of fantasy sports in Korea. The fantasy sports industry has expanded rapidly in the United States. However, there is growing concern of the fantasy sports: expectations are spreading for the new economic effects of the sports industry, along with the eyes of concern over illegal gambling. Therefore, the study analyzed the legal aspects of the fantasy sports in the United States and Korea to provide legislative direction for the fantasy sports’ implementation in Korea. Firstly, the study has reviewed the laws relating to the fantasy sports in the United States: Wire Act, Professional and Amateur Sports Protection Act, The Unlawful Internet Gambling and Enforcement Act and The Illegal Gambling Business Act. In addition, in order to review the possibility of institutionalization in Korea, relevant laws such as the Sports Industry Promotion Act, the National Sports Promotion Act, the Act on the Integrated Supervision of the gambling industry, the Act on Promotion of Games Industry, the Act on Promotion of Sports Promotion, and the Act on the Restriction of Casualties were analyzed. According to the analyses, fantasy sports have some potential to be considered illegal in the domestic law. Nonetheless, it could be institutionalized along with analysis and formation of regulations on reward system and participation limit, and the controversy of an inclination to gambling could be offset by publicness to fund National Sports Promotion Funding. The possible impacts on sports related industries were discussed and implications and suggestions were presented for the future direction of the legislation and institution.

네일리스트의 직무스트레스와 감정노동의 현상 연구

홍지은,이정현 한국인체미용예술학회 2014 한국인체미용예술학회지 Vol.15 No.3

Human aesthetic needs to pursue standardized and universal beauty and, at the same time, include the expression of individuals' personality and taste. Nail art is one of the many approaches to meet these aesthetic needs and demands. In this industry, many people have been challenged to start their own business because of easy access. In addition, it is likely that a new occupation called 'nailist,' which was derived through the specification and specialization of beauty services, would keep growing because of shop diversification and advantage of launching a business with a small budget. As a nailist, you need to provide face-to-face services, meaning that it would bring emotional labor. Therefore, job stress would be relatively high. Since a nailist's job requires close observation and a caring mindset, strong stamina and mental health are required in this industry. This study investigated the job stress and emotional labor of nailists in Busan, Gyeongnam, and Daegu and found the following: Job satisfaction was relatively high. In terms of job stress by education, the highest level was observed in the college group (undergraduate and graduate). In average monthly income, job stress was high in the group of relatively low income. In terms of emotional labor levels by average monthly income, a high level was detected in 'less than KRW 1million' and 'KRW 3 million or more.' In terms of emotional labor levels by job satisfaction, a relatively high level was found in the groups that responded 'dissatisfied' and 'not bad.' In addition, a statistically significant positive correlation was observed between job stress and emotional labor.