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      • KCI등재

        액체크로마토그래피-형광검출기를 이용한 닭고기 중 플루오로퀴놀론계 항균물질 정량분석 및 잔류조사

        박은정,임지흔,이성모 한국식품위생안전성학회 2004 한국식품위생안전성학회지 Vol.19 No.1

        1. 닭고기에서 4종의 플루오로퀴놀론계 합성항균제(ofloxacin, norfloxacin, ciprofloxacin, enrofloxacin)를 액상추출법으로 추출하여 형광검출기와 HPLC를 이용하여 동시 정량분석하는 방법을 확립하였으며 분석조건으로서 컬럼은 Symmetry C18(250×4.6 mm id, 5 ㎛), 이동상은 0.4% triethylamine 및 0.4% phospholic acid 수용액, methanol 및 acetonitrile 혼합용액(800:100:100, v/v/v)을 사용하였으며, 형광검출기는 여기파장 278 nm, 측정파장 456 nm으로 그리고 유속은 1.0 ml/min., 주입량은 50 ㎕로 하였다. 확립된 분석조건으로 측정한 ofloxacin, norfloxacin, ciprofloxacin, enrofloxacin 표준품의 표준곡선식에서 모두 상관계수 0.999이상의 양호한 직선성을 보였으며, 첨가한 닭고기의 크로마토그람에서도 각각의 nfwlfquf 분리시간대에 방해 피크 없이 양호한 분리도를 나타내었다. 0.05~0.2 ㎍/g 첨가한 시료에서 평균 회수율은 ofloxacin 92.0~95.4%, norfloxacin 84.2~87.3%, ciprofloxacin 78.3~82.2%, enrofloxacin 91.3~95.3%이었으며 변이계수(CV)는 2.7~9.4%이었다.4종의 동시분석법의 검출한계 및 정량한계는 각각 ofloxacin 23.5 ppb, 35.3 ppb, norfloxacin 3.4 ppb, 5.1 ppb, ciprofloxacin 3.0 ppb, 4.5 ppb, enrofloxacin 2.5 ppb, 3.8 ppb 수준이었다. 2. 인천 지역에서 돛구한 닭고기 총 1,523수를 EEC-4-plate법으로 검사한 결과 양성반응을 보인 닭고기는 15수(육계 10, 토종닭 5)였으며, HPLC를 이용한 정밀검사결과 육계 5수에서 ciprofloxacin이 불검출~0.04 ppm, enrofloxacin이 0.01~0.69 ppm수준으로 검출되었으며, 토종닭 5수에서는 ciprofloxacin이 0.02~0.12 ppm, enrofloxacin이 0.36~6.79 ppm수준으로 검출되었다. Ofloxacin, norfloxacin, ciprofloxacin, and enrofloxacin in chicken muscle were seperated by liquid extraction and determined with high performance liquid chromatography (HPLC) with fluorescence detector. Analysis was carried out using following cond itions; C18 column (250×4.6 mm i.d. 5 ㎛ particle size), mobile phase composed of D.W. (containing 0.4% triethylamine and phospholic acid): methanol : acetonitrile (800:100:100, v/v/v), isocratic pump at a flow rate of 1.0 ml/min and 50㎕ of injection volume, fluorescence detector with EX 278 nm/EM. 456 nm. The calibration curves of four fluoroquinolones showed linearity (r^2≥0.999) at concentration range of 0.025-0.6 ㎍/ml. The recoveries in fortified chicken muscle represented more than 80% with low coefficient of variation (<10%) for concentration range of four fluoroquinolones. The detection limits for ofloxacin, norfloxacin, ciprofloxacin, and enrofloxacin were 23.5, 3.4, 3.0 and 2.5 ng/g in chicken muscle, respectively. We also monitored fluoroquinolones residue in muscle of chickens (broiler 1,227, Korean native chicken 219, laying chicken 77) using EEC-4-plate screening and HPLC confirmation methods. Ten(broiler 5, Korean native chicken 5) out of the fifteen samples which were positively detected by EEC-plate screening method from 1,523 chicken meat were confirmed with ciprofloxacin and enrofloxacin by HPLC. The ranges of residual concentration were 0-0.12 ppm for ciprofloxacin and 0.01-6.79 ppm for enrofloxacin. In conclusion, our method could be applied effectively to determine four fluoroquinolones residues in chicken meat, and further survey for fluoroquinolones residue in chicken meat are needed for more effective control of fluoroquinolones used in livestock.

      • KCI등재

        토마토 과실에서 Superoxide Dismutase를 고발현하는 형질전환 식물체

        박은정,이행순,권석윤,최관삼,곽상수,Park, Eun-Jeong,Lee, Haeng-Soon,Kwon, Suk-Yoon,Choi, Kwan-Sam,Kwak, Sang-Soo 한국식물생명공학회 2002 식물생명공학회지 Vol.29 No.1

        Superoxide dismutase (SOD)를 과실에서 고발현시킨 형질전환 토마토 (서광과 꼬꼬)를 개발하였다. 카사바 배양세포에서 분리한 CuZnSOD (mSOD1)를 과실에 우세적으로 발현하는 ascorbate oxidase promoter (ASOp)를 이용하여 ASOp :: mSOD1/pBI101 벡터를 제작한 후 Agrobacterium 매개로 자엽 절편체를 형질전환하였다. Kanamycin 저항성 식물체를 기관발생 경로로 재분화시킨 후 Southern 분석으로 형질전환을 확인하였다. 서광과 꼬꼬 토마토의 형질전환체와 대조구 식물체의 과실을 성숙 단계별로 분류하여 단백질 함량과 SOD 비활성도 (units/mg protein)를 측정한 결과, 단백질 함량은 열매가 익은 단계로 갈수록 점점 감소하여 완전히 익은 단계에서 가장 낮았다. SOD 비활성도는 형질전환 토마토의 열매의 모든 단계에서 대조구보다 높았으며 완전히 성숙한 과실에서 가장 높았다. 성숙한 형질전환 서광과 꼬꼬 과실에서 SOD 비활성도는 비형질전환의 것보다 각각 약 1.6배와 약 2.2배 높았다. SOD isoenzyme gel 분석에서 도입한 mSOD1로 추정되는 CuZnSOD 밴드가 형질전환체에서 과실 성숙에 따라 강하게 발현되었다. 이상의 결과로서 ASO promoter에 의해 SOD 유전자가 토마토 과실에 특이적으로 발현됨이 확인되었다. Superoxide dismutase (SOD) plays an important role in cellular defense against oxidative stress in plants. We have developed transgenic tomato plants overexpressing a cassava SOD in fruits. Three transgenic tomato plants (one from cv. Pink forcer and two from cv. Koko) using a new vector system, ASOp :: . mSOD1/pBI101, harboring ascorbate oxidase promoter (ASOp) expressing dominantly in cucumber fruits, CuZnSOD cDNA (mSOD1) isolated from cultured cells of cassava, and nptll gene as a selectable marker were successfully developed. SOD specific activity (units/mg protein) in transgenic fruits of both cultivars was increased with maturation of the fruits. SOD specific activity of well-mature fruits in transgenic Pink forcer and Koko showed approximately 1.6 and 2.2 times higher than control fruits, respectively. The strength of SOD isoenzyme bands well reflected the SOD activity during the fruit maturation. These results suggested that SOD gene was properly introduced into tomato fruits in a fruit-dominant expression manner by ASO promoter.

      • 인간 폐포세포 유래 A549세포주에 대한 Mercuric oxide의 독성

        박은정,박광식 동덕여자대학교 종합약학연구소 2004 동덕약학연구지 Vol.8 No.-

        Heavy metals such as cadmium, mercury, chromium, lead and arsenic are known toxic to living organisms. However, studies on the sensitivity of toxicities among metals are not many. In the present study, we examine the cytotoxicities of mercuric oxide in cultured A549 cells, which are derived from human lung epithelia. The cells were also treated with three other different heavy metals, arsenite trioxide, cadmium chloride, and potassium chromate with various concentration. The relative cytotoxicity were measured by MTT and expressed as viablity ratio compared to control group at 24, 48, 72, and 96h. As results, mercuric oxide was found to be the most toxic among the metals compounds tested.

      • KCI등재후보
      • KCI등재
      • KCI등재

        중학생의 시간관리 : 관련변수 및 학업성취도와의 관계

        박은정,김외숙 한국가족자원경영학회 2008 가족자원경영과 정책 Vol.12 No.1

        The purpose of this study was to clarify variables affecting time management practice and the relationship between the practice and academic achievement of middle school students. Questionnaires including a time management scale were used for this study and the survey data were taken from 352 middle school students in Seoul and Gyeonggi-do. The statistical methods for the analysis were frequency, percentage, average, t-test, ANOVA and Pearson's correlation. The variables affecting student time management were gender, their standard of living, parental educational level and parental occupation. Religion and residential area were indicated as variables affecting several subscales of time management but the academic year of student had no effect on student time management behavior. The relationship between time management and the academic achievement of middle school students was slightly positive. Developing programs for time management in the field of family resource management is also necessary.

      • KCI등재
      • KCI등재

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