부산지역 무균성 뇌막염 원인 바이러스의 분리 및 동정 : 1998년을 중심으로

김영희,정영기,김기순,지영미,윤재득,김병준,구평태,민상기,정구영,김만수,조경순 동의대학교 기초과학연구소 2000 基礎科學硏究論文集 Vol.10 No.1

The incidence of aseptic meningitis infection is ensuing and threatening the health of children. Enteroviruses are the major agents of aseptic meningitis and identification of virus has been a clue to diagnosis and epidemiology. The outbreak of aseptic meningitis occurred in Pusan, 1998. Patients were concentrated from April through November. Children were more susceptible than adults. Among 306 cases of specimens from stool, throat swab tested, only 7.2% were positive on virus isolation, 12 cases from stool and 10 from throat, respectively. All isolated 7 serotypes of viruses represented cytopathic effect on cultured cells. Three types of echovirus 6, 25, 30 and coxsackievirus B2, B3, B4, B6 were identified by neutralizing anti body test. Isolated coxsackievirus and echovirus were observed by an electron microscope with negative staining.

서울의 Penicillinase Producing Neisseria gonorrhoeae 발생빈도(1998)

김재홍,김준호,반재용,이정우,황성주,정준규,정성태,강진문,조흔정,홍창의,정혜신,이한승,김이선,이봉길,이종호,선영우,한기덕,윤성필,이성훈,안종성,박석범,문승현,조항래,김형섭,류지호,황재영,박준홍,손상욱 한양대학교 의과대학 2001 한양의대 학술지 Vol.21 No.1

In recent years, gonorrhea has been pandemic and remains one of the most common STDs in the world, especially in developing countries. For the detection of a more effective therapeutic regimen and assessing the prevalence of Penicillinase Producing Neisseria gonorrhoeae(PPNG), we have been trying to study the patients who have visited the Venereal Disease Clinic of Choong-Ku Public Health Center in Seoul since 1980 by menas of the chromogenic cephalosporin method. In 1998, 93 strians of N. genorrhoeae were isolated, among which 60(64.5%) were PPNG. The prevalence of PPNG in Seoul, which had been decreased to 39% in 1996 after a peak of 74.3% in 1993, is increased to 64.5% in 1998.

제2형 당뇨병 남성 환자에서 유리 테스토스테론과 성호르몬 결합 글로블린 농도

남기덕,김영설,박철영,오승준,김덕윤,우정택,김성운,양인명,김진우,최영길 대한당뇨병학회 2002 Diabetes and Metabolism Journal Vol.24 No.6

연구배경:인슐린저항성은 제2형 당뇨병과 심혈관질환의 주요한 위험 인자로 성호르몬과 상호 관계가 있다고 알려져 있다. 그러나 여성과는 달리 남성에서는 연령과 인슐린저항성에 따른 유리 테스토스테론과 성호르몬 결합 글로블린 농도의 변화에 대한 연구가 부족한 실정이다. 그러므로, 본 저자등은 제2형 당뇨병 남성 환자에서 정산인과 비교해서 유리 테스토스테론과 성호르몬 결합 글로블린 농도를 측정하고 연령에 따른 변화 정도를 알아보고자 하였다. 방법:대상 환자 모두에서 연령과 체질량지수, 총 콜레스테롤, 중성지방, 공복혈당과 인슐린 농도를 측정하였다. 혈중 유리 테스토스테론 농도는 방사면역측정법(radioimmunoassay)을 이용해서 측정하였고, 혈중 성호르몬 결합글로불린은 면역방사계측측정법(immunoradiometric assay)을 이용해서 측정하였다(Diagnostic System Laoratories, Wbster, TX, USA). 결과:1)제2형 당뇨병 남성 환자에서 정상 대조군 남성에 비해 성호르몬 결합글로불린은 104.1±35.0 vs 25.7±3.5 mole×10??로 의미 있게 높았으나(p<0.001), 유리 테스토스테론은 13.7±9.5 vs 13.6±6.5 ng/dL로 차이가 없었다. 2)연령과 성호르몬 결합 글로블린 사이의 상관 계수는 제2형 당뇨병 남성 환자에서 0.40로 중등고의 양의 상관 관계를 보였고(p<0.001), 정상 대조군 남성에서 0.11로 유의한 상관 관계를 보이지 않았다. 연령과 유리 테스토스테론 사이의 상관 계수는 제2형 당뇨병 남성 환자에서 0.08, 정상 대조군 남성에서-0.17로 모두에서 유의한 상관 관계를 보이지 않았다. 3)연령과 체질량지수를 보정한 후에 제2형 당뇨병 남성 환자와 정상 대조군 남성에서 혈중 인슐린 농도, 유리 테스토스테론과 성호르몬 결합 글로블린 사이에는 상관 관계가 없었다. 결론:제2형 당뇨병 남성 환자에서 정상 남성에 비해 성호르몬 결합 글로블린 농도가 증가되어 있었으며, 유리 테스토스테론은 차이가 없었다. 연령이 증가함에 따라 성호르몬 결합 글로블린이 제2형 당뇨병 남성 환자에서 정상 남성에 비해 증가 폭이 의미있게 컸으며, 유리 테스토스테론은 변화가 없었다. Background: Insulin resistance is a risk factor for cardiovascular disease and type 2 diabetes mellitus. There are many previous studies indicating that insulin lowers serum sex hormone-binding globulin levels, and there is inverse correlation between insulin resistance and serum sex hormone-binding globulin levels in women. However, in men, a limited number of studies are available to explain the effect of sex hormone on age and insulin. Therefore, the present study was undertaken to investigate the relationship among free testosterone, hormone-binding globulin and age in type 2 diabetic men and control subjects. Method: Age, body mass index, total cholesterol, triglyceride, fasting blood sugar, and insulin concentrations were examined on 89 type 2 diabetic men and 47 control subjects. The free testosterone level was measured by commercially available double-antibody system (Radioimmunoassay). The sex hormone-binding globulin level was also measured by commercially available double-antibody system(Immunoradiometric assay). Results: 1) Sex hormone-binding globulin level was significantly increased in patients with type 2 diabetes. However, there was no significantly difference in free testosterone level between the two groups. 2) Sex hormone-binding globulin was positively correlated with age (r=0.4, p <0.001) in patients with type 2 diabetes. Sex hormone-binding globulin and free testosterone were not correlated with age in control sujects. 3) Free testosterone and sex hormone-binding globulin concentrations were not significantly related to serum insulin concentration after adjusting for age and body mass index. Conclusions: We observed increased sex hormone-binding globulin concentration in diabetes man, and was a positively related to age. Further studies are needed to understand the relationships between age, insulin resistance, testosterone, and sex hormone-binding globulin concentrations(J Kor Diabetes Asso 24:699~707,2000).

유산양 유즙으로부터 분리된 세균의 분포 및 항균제 감수성 검사

김혜라 ( Hye Ra Kim1 ),정지영 ( Ji Young Jung ),김선득 ( Seon Deuk Kim ),박준영 ( Jun Young Park ),조인영 ( In Young Cho ),신성식 ( Sung Shik Shin ),손창호 ( Chang Ho Son ),오기석 ( Ki Seok Ok ),허태영 ( Tai Young Hur ),정영훈 ( Y 한국동물위생학회 2012 韓國家畜衛生學會誌 Vol.35 No.4

The aim of this study was to isolate microorganisms from half milk samples of dairy goats by California mastitis test (CMT) during the lactation period and to further investigate the susceptibility of isolated organisms to antimicrobial drugs. From a total of 235 half milk samples with CMT scores of 2 or above from 366 dairy goats distributed throughout Jeonnam province, microorganisms were isolated from 198 (83.5%) samples either singly (99.0%) or in combination (1.0%). The most prevalent microorganism was the coagulase-negative Staphylococcus spp., (44.4%, n=88) followed by Staphylococcus aureus (24.2%, n=48), Escherichia coli (11.1%, n=22) and Streptococcus spp. (7.6%, n=15). Isolated bacteria also included Bacillus spp. (2.5%, n=5), Pseudomonas spp. (2.5%, n=5), Micrococcus spp. (1.5%, n=3), Corynebacterium spp. (1.5%, n=3), Enterococcus facium (1.0%, n=2), Morganella morganii (0.5%, n=1) and Streptococcus agalactiae (0.5%, n=1). During the summer season, a high prevalence of all micro-organisms were observed in which Staphylococcus spp. (30.8%), Escherichia coli (8.6%), and Streptococcus spp. (5.6%) were among the most prevalent bacteria isolated. Staphylococcus spp. was also shown to be high in the winter (21.7%). In most samples, the presence of bacterial pathogens in goat milk led to the increase in the total somatic cell count (SCC). Most of the half milk samples of dairy goats with bacterial contamination showed SCC of ≥1×10(6) cells/ml (90.4%). Minor pathogens (11.4%) were more detected from milk samples with SCC of <1×10(6) cells/ml than major pathogens (4.1%), while the major pathogens tended to be higher from samples with SCC of ≥3×10(6) cells/ml. Susceptibility of these bacteria to 12 antimicrobial agents was tested by the Kirby-Bauer disc diffusion method. Results indicated that more than 90% of bacteria isolated from CMT 2+ dairy goat half milk samples were susceptible to trimethoprim/sulfamethoxazole, amoxicillin/clavulanic, enrofloxacin and cephalothin while they were resistant to tetracycline (44.7%).

경부고속철도와 일본 도카이도신간선의 비교 연구

김성득,류영언 울산대학교 1997 공학연구논문집 Vol.28 No.1

우리나라 남·북축을 잇는 경부고속철도와 일본 도카이도신간선의 건설배경, 건설효과 및 노선의 확충에 대해 비교고찰하고 관련 전문가가 소속된 학회 회원들과 지역 여론을 설문조사하고 분석하였다. 우리나라 경부고속철도의 중간역수와 역간 평균거리는 일본 도카이도신간선의 중간역수, 역간 평균거리와 비교하여 많은 차이가 남을 알 수 있었다. 경주·울산권에 1개의 중간역을 설치하여 광역도시권으로 함께 발전하는 안과 2개의 중간역을 설치하여 2개의 도시권으로 발전하는 안에 대한 경주, 울산, 포항 세 도시의 발전 방향에 대한 면밀한 검토가 있어야 하겠다. 설문조사 결과 경주 중간역사 위치 여하에 따라 울산 중간역사 설치의 강도가 달라졌고, 경주 중간역사 설치 위치와 원활한 연계교통망 건설이 세 도시의 21세기 발전과 국가발전에 크게 영향을 미칠 것이라 판단되었다. This paper presents a comparative study on the Seoul-Pusan High-Speed Railroad and the Tokaido Shinkansen of Japan. The comparitive study of the two areas includes construction backgrounds, development, construction effect, and line expansion. Questionnaire surveys were carried out to collect opinions from experts and local residents. The projects sound much different in the number of intermediate stations and average intervals between two adjacent stations. Two different approaches were suggested in Kyungju area, one big city with one intermediate station. Based on these two approaches, three cities have much different impacts on future developments. The survey shows that the need of the Ulsan intermediate station depends on the location of Kyungju intermediate station. It is found that the Kyungju intermediate station and smooth traffic networks are very strongly related to the 21'C development of Kyungju, Ulsan, and Pohang, three cities and the nation.

二重 Langevin 振動子의 變壓特性에 관한 硏究

金漢星,任漢錫,韓得永 건국대학교 부설 산업기술연구소 1982 논문집 Vol.7 No.-

This paper suggests a new model of piezoelectric transformer fabricated with steel plates, piezoelectric ceramics, electrodes and thin insulators. And the transformation ratio and the phase difference of several kinds of those transformers were discussed. As a result, the transformation ratio can be increased by series connection of the output ceramic elements or parallel connection of the point ceramic elements. Phase difference between the input voltage and the output voltage was occurred by 90˚ when the transformer had the maximum transformation ratio. These characteristics seem to be applied to develop the high frequency power transformers.

상추의 생산단계별 Chlorpyrifos 및 Procymidone의 잔류허용기준 설정

김영숙,박주황,박종우,이영득,이규승,김장억 한국환경농학회 2002 한국환경농학회지 Vol.21 No.2

상추의 생산단계에서의 잔류허용기준 설정과 최종 소비단계에서의 안전성 평가자료로 활용하기 위하여 지금까지 상추수확물에서의 잔류수준과 검출빈도가 높은 것으로 알려진 살충제 chlorpyrifos 및 살균제 procymidone의 포장상태에서의 생물학적 반감기, 보관상태에 따른 잔류량 변화 그리고 세척형태에 따른 제거율을 조사하였다. 시설재배포장에서 상추중 두 약제의 살포농도별 생물학적 반감기를 측정한 결과 기준량 살포의 경우 chlorpyrifos 1.2일 및 procymidone 1.3일, 배량살포의 경우에는 chlorpyrifos 1.5일 및 procymidone 2.6일로 나타났다. 유통과정중에서 일어날 수 있는 두 가지 농약의 잔류량 변화를 알아보기 위해서 실온(20℃) 및 냉장(4℃) 상태로 보관하면서 두 약제의 잔류량 변화를 조사한 결과 반감기는 실온저장시 chlorpyrifos 3.8일, procymidone 7.2일이었고, 냉장저장시에는 chlorpyrifos 12.4일, procymidone 20.9일로 나타나 두 약제 모두 실온저장보다 냉장저장시 잔류량의 감소가 훨씬 느린 것으로 나타났다. 세척방법에 의한 잔류농약의 감소량을 조사한 결과 세제 사용시의 평균 제거율은 chlorpyrifos 48.3%, procymidone 52.6%로 나타났고, 물 사용시에는 chlorpyrifos 31.3%, procymidone 38.1%로 나타났다. Two pesticides commonly used for lettuce cultivation including chlorpyrifos and procymidone were subjected to a field residue trial to ensure safety of terminal residues in the harvest. After pesticides were applied at standard and double rates in a foliar spray, leaf persistence of their residues was investigated for 10 days prior to harvest. Even though far exceeded the tolerances, initial leaf residues were rapidly dissipated with time and remained only 0.4~7.2% of the residues in the harvest. As well fitted by the first-order kinetics, biological half-lives of the pesticide residues in lettuce leaves ranged 1.2~2.6 days. Slow dissipation of the residues in the harvest was observed during storage at room temperature and 4℃ for 7 days. Portions of dislodgeable residues which resided in detergent washings decreased as time elapsed. Patterns in dissipation and distribution of dislodgeable residues were not largely affected by the application rate of pesticides. It is concluded that timing of pesticide application, that is, pre-harvest interval would be the first factor to determine the terminal residue level in edible portions of lettuce.

산소-질소계 여러 자리 시프염기리간드를 이용한 구리(II)의 비색정량

김선덕,김영숙,김준광 대구대학교 기초과학연구소 1997 基礎科學硏究 Vol.14 No.1

The colorimetric determination of Cu(II) ion by using the polydentate schiff base ligands Bis-(salicylaldehyde)-diethylentriamine(BSDT), Bis- ( salicylaldehyde) -triethylenetetramine (BSTT), and Bis-(salicylaldehyde)-tetraethylenepentamine(BSTP) have been developed. The schiff bases and their Cu(II) complexes were obeyed to Beer's law in the concentration range 1.0X 10-3-4.OX 10 3M. The molar extinction coefficients of the Cu(II) complexes are low, and therefore the sensitivity is relatively low. Cu(ID was determined in the presence of univalent ions and bivalence cation ions but oxlate, EDTA, Fe(II), Fe(III) and Co(II) were interfered.

만성 심부전환자의 혈액에서 측정한 Tumor necrosis factor-α 및 Interleukin-6의 임상적 의의

김명구,김성구,박상호,한대희,강진환,변정득,심규혁,최병조,방덕원,온영근,현민수,권영주 순천향의학연구소 2001 Journal of Soonchunhyang Medical Science Vol.7 No.2

Background and objectives: Many conditions are responsible for the pathophysiology and progressive mechanisms of congestive heart failure. More recently, it has also become evidence that another class of biologycally activated molecules generically reffered to as cytokine these are also over expressed in congestive heart failure. Therefore, the purpose of this study was to measure concentrations of tumor necrosis factor-α(TNF-α), interleukin-6(IL-6) in mild to severe symptoms of heart failure and compare their values with those found in normal control and analysed correlation relationship between cytokine level, clinical findings and hemodynamic indicies. Subjects and Methodology: Levels of TNF-α and IL-6 were measured on pulmonary artery during cardiac catheterization in heart failure patients(n=32) and normal subjects(n=8) as well as physical examination and echocardiogram. Cytokines assay were performed on plasma using commercially available ELISA(Enazyme-Linked Immunosorbent Assay) kits. Results: Although the levels of TNF-α and IL-6 tend to increase in congestive heart failure group, the cytokines level was not made significantly statistical difference between congestive heart failure group and controls. When analyzing the correlation between the levels of PCWP(pulmonary capillary wedge pressure) and cytokines(TNF-α, IL-6), respectively, there were statistically significant correlation coefficient 0.32,(p<0.05), 0.39(p<0.01). The cytokine IL-6 and pressure of pulmonary artery were significant correlation.(correlation coefficient 0.36, p<0.02) More significantly, there was correlated with TNF-α and IL-6.(correlation coefficient 0.57, p<0.001) Conclusions: There was tended toward high concentration of TNF-α & IL-6 in congestive heart failure and significant difference for PCWP between TNF-α & IL-6, thus may be correlated with development and progression in congestive heart failure.

AlGaAs/GaAs계 VSIS 레이저 다이오드의 종방향 특성해석을 위한 Simulation

김득영 동국대학교 산업기술연구원 1997 산업기술논문집 Vol.9 No.-

GaAs계 정보처리용 고출력 반도체 레이저 다이오드의 소자설계를 위한 시뮬레이션을 수행하였다. 본 연구에 이용된 레이저 다이오드의 구조는 굴절률 도파형의 V-grooved substrate inner stripe 구조이며, 광출력, 활성층의 두께 및 광도파층의 두께 등을 변수로 설정하였다. 시뮬레이션은 이들 변수에 의한 유효굴절률, 소광계수, 이득분포 및 필드패턴 등의 특성을 레이저 다이오드 구조에서의 종방향 거리에 따라 분석하는 방법으로 수행하였다. 도파층과 활성층의 두께가 일정할 때 광출력이 높아질수록 중심에서의 유효굴절률은 높아지고 소광계수는 낮아짐을 알 수 있었다. 근시야상의 세기는 광출력, 활성층의 두께 및 도파층의 두께 변화에 따라 중심에서의 값이 다르게 나타났으나 원시야상의 세기는 이들 조건에 무관하게 중심에서는 일정한 값으로 나타났다. In this work, the index guided type of AlGaAs/GaAs laser diode was simulated for device modeling. The V-grooved substrate inner stripe structure was used as a modeling structure. The output power, thickness of active layer, and thickness of guided layer were varied as a simulation parameters. The equivalent refractive index, extinction coefficient and field patterns are simulated as a function of lateral distance using these simulation parameters. Under constant thickness of active layer and guided layer, equivalent index is increase and extinction coefficient is decrease for high output power. The intensities of near field pattern are different at center with variation of parameters, howerver the intensities of far field pattern are same having a no connection with simulation parameters.