삼보감(Citrus sulcata Hort. et Takahashi) 유실물체에서 유도된 캘러스의 체세포배 형성과 식물체 재분화

박수영,허인옥,부지현,한태완,송관필 濟州大學校 基礎科學硏究所 1998 基礎科學硏究 Vol.11 No.1

삼보감에 있어서 기내 체세표배 발생을 통한 다량증식을 도모하기 위해서 캘러스 증식 및 체세포배 발생, 배로부터 식물체 재분화에 미치는 배지 및 Polyamine, 생장조절제의 효과를 구명하기 위해 실시하였다. 삼보감 과실에서 채집된 종자를 호르몬이 첨가되지 않은 MS배지에서 무균발아시켰다. 발아된 유식물체의 줄기에서 캘러스를 유도하기 위해 NAA와 BA가 첨가된 MT 배지에서 배양하였고 5mg/L 2,4-D와 1mg/L BA가 첨가된 배지에서 계대배양하였다. 캘러스를 증식시키기 위한 배양조건 설정으로 배지별과 Polyamine(Spermidine, Spermine, Putrecine) 농도별(0 - 1mM)에 따른 생장량을 좌하였다.배지별에 따른 생장율은 MT배지에서 0.968g(fr wt)으로 가장 높았고, Polyamine의 영향은 0.01 mM Putrecine 처리구에서 0.78g으로 가장 높게 나왔다. 배발생 캘러스는 0.1mg/L NAA와 0.5mg/L BA가 첨가된 MT배지에서 유도하였으며 유되된 체세포배를 식물체로 재분화시키기 위해서 0.1mg/L NAA와 1mg /L BA가 첨가 된 MT배지에서 배양하였다. This study was performed to investigate the culture condition induction of somatic embryo and plant regeneration in callus induced from Citrus sulcata leaf and stem as a basic research for breeding of new plant. The seeds of Sambokam were germinated in hormone free MS medium under sterile condition. Callus induced form stem and leaf geminated young plant in MT supplemented with NAA plus BA and subcultured in MT supplemented with 5 ㎎/ L 2,4-D and 1 ㎎/L BA. As a investigation of culture condition for callus proliferation, growth rate of callus were investigated in various medium and polyamine concentrations. The effect of medium was most effective in MT medium as 0.968g and polyamine was most effective of in 0.01mM putrecine among various concentration. Formation of embryogenic callus induced from MT medium containing 0.1 ㎎/L and 0.5 ㎎/L BA. The geminated embryos developed to complete plantlet when cultured on MT medium supplemented with 0.1 ㎎/L NAA and 1㎎/L BA.

하계 실내 및 실외환경의 공기 중 휘발성 유기화합물 농도 및 개인노출

양원호,손부순,박종안,장봉기,박완모,김윤신,어수미,윤중섭,류인철 한국환경과학회 2003 한국환경과학회지 Vol.12 No.9

Volatile organic compounds (VOCs) are present in essentially all natural and synthetic materials from petrol to flowers. In this study, indoor and outdoor VOCs concentrations of houses, offices and internet-cafes were measured and compared simultaneously with personal exposures of each 50 participants in Asan and Seoul, respectively. Also, factors that influence personal VOCs exposure were statistically analyzed using questionnaires in relation to house characteristics, time activities, and health effects. All VOCs concentrations were measured by OVM passive samplers (3M) and analyzed with GC/MS. Target pollutants among VOCs were Toluene, o-Xylene, m/p-Xylene, Ethylbenzene, MIBK, n-Octane, Styrene, Trichloroethylene, and 1,2-Dichlorobenzene. Indoor and outdoor VOCs concentrations measured in Seoul were significantly higher than those in Asan except Ethylbenzene. Residential indoor/outdoor (I/O) ratios for all target compounds ranged from 0.94 to 1.51 and I/O ratios of Asan were a little higher than those of Seoul. Relationship between personal VOCs exposure, and indoor and outdoor VOCs concentrations suggested that time-activity pattern could affect the high exposure to air pollutant. Factors that influence indoor VOCs level and personal exposure with regard to house characteristics in houses were building age, inside smoking and house type. In addition insecticide and cosmetics interestingly affected the VOCs personal exposure. Higher exposure to VOCs might be caused to be exciting increase and memory reduction, considering the relationship between measured VOCs concentrations and questionnaire (p<0.05).

서울의 Penicillinase Producing Neisseria Gonorrhoeae 발생빈도(1996)

김재홍,황동규,전재홍,김윤석,김중환,김용준,이창균,임동진,김현수,조창근,김경문,박상훈,전우형,김희성,이호정,차명수,김갑형,김형석,김석우,황지환,박병순,권오상,이민수,송기훈,성소영,이인섭,부태성 대한화학요법학회 1999 대한화학요법학회지 Vol.17 No.2

Background : In recent years, gonorrhea has been panedemic and remains one of the most commom STDs in the world, especially in developing countries. Objective & Methods: For the detection of a more effective therapeutic regimen and assessing the prevalence of PPNG, we have been trying to study the patients who have visited the VD Clinic of Choong-Ku Public Health Center in Seoul since 1980 by means of the chromogenic cephalosporin method. Results: In 1996, 139 strains of N. gonorrhoeae were isolated, among which 53(39.0%) were PPNG. Conclusion: Our results suggests that after a peak of 74.3% in 1993, the prevalence of PPNG in Seoul is gradually declining.

Composition for detecting β-1,3-glucan, preparation method thereof and diagnostic kit detecting β-1,3-glucan

Bu-Soo Park(박부수),Yong-Soon Hwang(황용순),Jeong-Min Kim(김정민),Jong-Won Yoon(윤종원) 한국생물공학회 2005 한국생물공학회 학술대회 Vol.2005 No.10

Single Oral Dose Toxicity Study of Prebrewed Armeniacae Semen in Rats

Park, Ji-Ha,Seo, Bu-Il,Cho, Su-Yeon,Park, Kyu-Ryul,Choi, Seung-Hoon,Han, Chang-Kyun,Song, Chang-Hyun,Park, Soo-Jin,Ku, Sae-Kwang Korean Society of ToxicologyKorea Environmental Mu 2013 Toxicological Research Vol.29 No.2

Armeniacae semen (AS) has been considered a toxic herb in the Korean medicine as it contains hydrogen cyanide and amygdalin, especially in its endocarp. Therefore, prebrewed AS that is devoid of endocarp has been traditionally used. In the present study, amygdalin content of the prebrewed AS was significantly lower ($2.73{\pm}0.32{\mu}g/ml$; p<0.01) than the content in the extract that contained the endocarps ($28.50{\pm}6.71{\mu}g/ml$); amygdalin content corresponded to 10% of the extract in the present study. Because of single oral dose toxicity of prebrewed AS according to the recommendation of Korea Food and Drug Administration Guidelines (2009-116, 2009), which was based on single oral dose toxicity study of prebrewed AS, mortality due to toxic principles was significantly reduced. In this study, 2,000 mg/kg of prebrewed AS led to death of 1 female rat and 1 male rat at the end of 2 hr of administration. Based on these results, the 50% lethal dose in both male and female rats was determined to be 9279.5 mg/kg. Seizure, loss of locomotion, and increases in respiration and heart rate were observed as prebrewed AS treatment-related toxicological signs; these signs were restrictedly manifested in the prebrewed AS (2,000 mg/kg)-treated rats. In addition, no changes were observed in body weight, organ weight, gross features, and histopathological parameters with 2,000 mg/kg of AS in both male and female rats. These findings serve as direct evidence that amygdalin in AS is the toxic principle, which can be reduced by the traditional prebrewing method involving the exclusion of endocarp.

Aspergillus niger를 이용한 글루콘산 나트륨 생산 생변환 공정의 최적화

박부수(Bu-Soo Park),조병관(Byung-Kwan Cho),이상윤(Sang-Yoon Lee),임승환(Seong-Hwan Lim),김동일(Dong-Il Kim),김병기(Byung-Gee Kim) 한국생물공학회 1999 KSBB Journal Vol.14 No.3

Protective Effect of Herbal Extracts against Skin Wrinkle Induced by UVB Irradiation in Hairless Mice

Jong Soo Kim, Bu-Hui Bae, Hyun Ji Park, Bong Su Kang, Dang-Young Kim, Seon-Young Hwang, Byung Kuk Ahn, Sang Yoon Nam, Young Won Yun, Beom Jun Lee 충북대학교 동물의학연구소 2010 Journal of Biomedical and Translational Research Vol.11 No.3

본문참고

Single Oral Dose Toxicity Study of Prebrewed Armeniacae Semen in Rats

Ji-Ha Park,Bu-Il Seo,Su-Yeon Cho,Kyu-Ryul Park,Seung-Hoon Choi,Chang-Kyun Han,Chang-Hyun Song,Soo-Jin Park,Sae-Kwang Ku 한국독성학회 2013 Toxicological Research Vol.29 No.2

Armeniacae semen (AS) has been considered a toxic herb in the Korean medicine as it contains hydrogen cyanide and amygdalin, especially in its endocarp. Therefore, prebrewed AS that is devoid of endocarp has been traditionally used. In the present study, amygdalin content of the prebrewed AS was significantly lower (2.73 ± 0.32 μg/ml; p < 0.01) than the content in the extract that contained the endocarps (28.50 ± 6.71 μg/ml); amygdalin content corresponded to 10% of the extract in the present study. Because of single oral dose toxicity of prebrewed AS according to the recommendation of Korea Food and Drug Administration Guidelines (2009-116, 2009), which was based on single oral dose toxicity study of prebrewed AS, mortality due to toxic principles was significantly reduced. In this study, 2,000 mg/kg of prebrewed AS led to death of 1 female rat and 1 male rat at the end of 2 hr of administration. Based on these results, the 50% lethal dose in both male and female rats was determined to be 9279.5 mg/kg. Seizure, loss of locomotion, and increases in respiration and heart rate were observed as prebrewed AS treatment-related toxicological signs; these signs were restrictedly manifested in the prebrewed AS (2,000 mg/kg)-treated rats. In addition, no changes were observed in body weight, organ weight, gross features, and histopathological parameters with 2,000 mg/kg of AS in both male and female rats. These findings serve as direct evidence that amygdalin in AS is the toxic principle, which can be reduced by the traditional prebrewing method involving the exclusion of endocarp.

장기간 홍삼복방제 투여가 혈중 지질 및 체지방 변화에 미치는 영향

김성수(Sung-Soo Kim),박호윤(Ho-Yoon Park),변용현(Yong-Hyun Byun),황부근(Bu-Geun Hwang),이재현(Jae-Hyun Lee),심영제(Young-Jae Shim),박채규(Chae-Kyu Park),박명한(Myung-Han Park),양재원(Jai-Won Yang) 고려인삼학회 2002 Journal of Ginseng Research Vol.26 No.2

장기간의 홍삼복방제 투여가 혈중지질 및 체지방에 미치는 영향을 알아보고자 체지방율이 30% 이상인 여학생 28명을 대상으로 위약군(placebo group, n=7명), 홍삼복방제투여군 (red-ginseng product administration group, n=7명), 운동군(exercise group, n=7명), 운동 및 홍삼복방제투여군(exercise<br/> and red-ginseng product administration group, n=7명) 등 네 집단으로 분류하여 12주간의 실험기간에 따른 TC, TG, HDL-C, LDL-C, 체중 및 체지방의 변화를 관찰하여 분석한 결과 다음과 같은 결론을 얻었다. TC, TG, LDL-C의 경우 실험 12주후 위약군을 제외한 모든 집단에서 유의한[홍삼복방제투여군(p<.001), 운동군(p<.01), 운동 및 홍삼복방제투여군(p<.001)] 감소를 보였다. HDL-C의경우실험 12주후 위약군을 제외한 모든 집단에서 유의하게[홍삼복방제투여군(p<.05), 운동군(p<.01), 운동 및<br/> 홍삼복방제투여군(p<.001)] 증가하였다. 체중, 체지방율, 체지방량의경우실험 12주후 위약군을 제외한 모든 집단에서 유의한[홍삼복방제투여군(p<.001), 운동군(p<.001),운동 및홍삼복방제투여군(p<.001)] 감소를 보였다. 제지방량의 경우 실험 12주후 위약군을 제외한 모든 집단에서 유의하게[홍삼복방제투여군(p<.05), 운동군(p<.001), 운동 및 홍삼복방제투여군(p<.001)] 증가하였다. This study was done in order to evaluate the effects on the blood lipid profiles, the body weight and body fat in 28 healthy female volunteers who had over 30% body fat by the long term intake of red ginseng product. Subjects were divided into four groups (placebo group n=7, red ginseng product group; n=7, exercise group; n=7, exercise & red ginseng product group; n=7). Blood sampling and measuring of the body fat were taken by pre-treatment, 3 weeks, and after 12 weeks.Statistical techniques for data analysis were applied one-way ANOVA and repeated measures ANOVA. The 5% level of significance was used as the critical level for this study. In summary of results, total<br/> cholesterol, triglyceride and low density lipoproprotein cholesterol were reduced in three groups (red ginseng product group, p<0.001; exercise group, p<0.01; exercise & red ginseng product group, p<0.001) except placebo group. HDL-C was improved in three groups (red ginseng product group, p<0.05; exercise group, p<0.01; exercise & red ginseng product group, p<0.001) except placebo group. Body weight, percent body fat and body fat mass were reduced in three groups (red ginseng product group, p<0.01; exercise group, p<0.01; exercise & red ginseng product group, p<0.001) except placebo group. Finally, lean body mass was improved in three groups (red ginseng product group, p<0.05; exercise group, p<0.01; exercise & red ginseng product group, p<0.001) except placebo group.<br/> Key words : Red ginseng, blood lipid, body fat, total cholesterol, triglyceride, low density lipoprotein cholesterol (LDLC), high density lipoprotein cholesterol (HDL-C)

Porphyromonas gingivalis에 대한 노각나무 잎 추출물의 항균활성 및 생물막 형성 억제 효과

김혜수(Hye Soo Kim),박민정(Min Jeong Park),김수정(Soo Jeong Kim),김부경(Bu Kyung Kim),박준호(JunHo Park),김대현(DaeHyun Kim),조수정(Soo Jeong Cho) 한국생명과학회 2021 생명과학회지 Vol.31 No.3

본 연구에서는 천연물유래 구강건강 개선소재로써 노각나무의 이용 가능성을 알아보기 위해 노각나무 잎과 줄기를 에탄올에 추출한 다음 구강미생물에 대한 추출물의 항균활성을 조사하였다. 노각나무 잎과 줄기 추출물(1 mg/disc)은 구강미생물 중 P. gingivalis KCTC5352에 대해서만 항균활성을 나타내었으며 줄기보다는 잎 추출물의 항균활성이 우수하였다. 시판되고 있는 구강케어제품에 사용되고 있는 항균제와 노각나무 잎 추출물의 항균활성을 비교한 결과, P. gingivalis에 대한 노각나무 잎 추출물과 양성대조구로 사용한 triclosan의 항균활성은 유사하게 나타났으며. P. gingivalis에 대한 노각나무 잎 추출물의 MIC는 0.4 mg/ml이고 정균작용을 하였다. 노각나무 잎 추출물이 0.2-2.0 mg/ml 농도로 처리된 배양액에서 P. gingivalis KCTC5352의 생물막 형성과 세균 생육은 추출물의 농도가 증가할수록 농도의존적으로 억제되는 경향을 보였다. 또한 노각나무 잎 추출물(1 mg/ml) 처리가 P. gingivalis의 생물막 형성에 미치는 영향을 주사전자현미경으로 관찰한 결과에 의하면 추출물을 처리하지 않은 대조구는 추출물 처리구에 비해 P. gingivalis가 군집을 이루며 모여 있었고 세포 주변에서 생물막이 관찰되었지만 추출물을 처리한 처리구의 세포 주변에서는 생물막을 관찰할 수 없었다. qRT-PCR을 이용하여 생물막 형성 초기 과정에서 치면 부착에 필수적인 섬모(fimbriae)관련 mRNA 발현 양상을 0조사한 결과, 노각나무 잎 추출물이 0.2-2.0 mg/ml의 농도로 처리된 배양액에서 fimA와 mfa1 유전자 발현은 추출물의 농도가 높아질수록 농도의존적으로 억제되는 것을 확인할 수 있었다. 이상의 결과를 종합하면 노각나무 잎 추출물은 치주질환 원인균인 P. gingivalis에 대한 항균 활성과 생물막 형성 억제능이 우수하기 때문에 천연물유래 구강건강 개선소재로써 이용 가능성이 높을 것으로 판단된다. This study was conducted to investigate the potential of Stewartia koreana as oral healthcare materials. The antibacterial activity of ethanol extracts from leaves and branches of S. koreana against oral bacteria was confirmed. The leaf and branch extracts (1 mg/disc) showed antibacterial activity against P. gingivalis only among several tested oral bacteria. The leaf extracts showed higher antibacterial activity, with values similar to those of chlorhexidine, which was used as a positive control. The MIC of the leaf extract against P. gingivalis was 0.4 mg/ml and showed bacteriostatic action. The inhibitory effects of the extract on biofilm formation and on gene expression related to biofilm formation by P. gingivalis were determined by biofilm biomass staining, scanning electron microscopy (SEM), and qRT-PCR analysis. The biofilm production rate and cell growth of P. gingivalis in the cultures treated with 0.2-2.0 mg/ml of S. koreana leaf extracts were significantly decreased in a concentration-dependent manner. The inhibitory effect on the formation of P. gingivalis biofilms at concentrations of 1 mg/ml was confirmed by SEM. The qRT-PCR analysis showed concentration-dependent suppression of the fimA and fimB gene expression associated with fimbriae formation in the cultures treated with 0.2-2.0 mg/ml S. koreana leaf extract. These results support the conclusion that S. koreana leaf extracts can be used as oral healthcare materials derived from natural materials, as demonstrated by the antibacterial action and inhibition of biofilm formation of P. gingivalis.