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The level of urinary aflatoxin M1 in Korean adults
김용대,권효진,문선인,엄상용,박정덕,최병선,손석준,홍영섭,김호,권호장,임지애,윤해정,김광진,김헌 충북대학교 동물의학연구소 2012 Journal of Biomedical and Translational Research Vol.13 No.3
Competitive ELISA methods was used to measure the level of aflatoxin M1 (AFM1) from urine in 1008 of Korean adults. Subjects were selected by random sampling in all area of Korea except Cheju-do. The recovery rate of AFM1 in this method was 105% (73-124%). The geometric mean of urinary AFM1 in all subjects was 3.43 pg/mL (3.67 ng/ g creatinine). The level of AFM1 in male showed statistically higher than one in female subjects. However, the levels of AFM1 were not different according to their age. The subjects in Chungbuk-do showed the highest urinary AFM1 concentration, whereas the subjects in Kyeongnam-do showed the lowest one. Assuming an excretion rate of 5%, this AFM1 excretion corresponds to about a 0.1 microgram/day in Korean adults.
Effects of onion peel water extracts on swimming endurance in rat
한상준,최준호,홍선화,박상호,정영호,김옥진 충북대학교 동물의학연구소 2013 Journal of Biomedical and Translational Research Vol.14 No.2
Onion (Allium cepa L.) contains high levels of dietary fibers and antioxidants, including vitamin C, D, and folates. Onion is also known as a quercetin-rich vegetable with high flavonoid content. Onion peel contains over 20 times more quercetin than onion flesh. The aim of this study was to examine the question of whether onion peel extract supplementation has an effect on maximal exercise performance in rat. Onion peel extracts were extracted with hot water. Thirty male Sprague Dawley rats were maintained on a pellet diet for one week, and then randomly divided into five groups: Normal control, Positive control (quercetin 20 mg/kg), Onion peel 4 mg/kg, Onion peel 20 mg/kg, and Onion peel 100 mg/kg. Oral administration was performed daily. The experimental period was four weeks. Thereafter, animals were then forced to swim in water and the maximal exercise performance period from the swimming start time to the exhausted time, in which they failed to rise to the surface of the water to breathe within a 7 second period, was measured. After necropsy, weights of gastrocnemius muscles were measured. Lactate dehydrogenase concentration in serum was measured using an enzymatic method, using a commercial kit. The maximal exercise performance period was significantly longer in the onion peel extracts fed groups, compared with the control group. The lactate dehydrogenase concentration of the onion peel extracts fed groups was significantly lower, compared with the control group. Based on these results, we suggest that onion peel water extract supplementation can enhance exercise capacity caused by the mechanism of decreasing lactate dehydrogenase concentration.
문호진,Mohammed Y. E. Chowdhury,김철중,신광순 충북대학교 동물의학연구소 2013 Journal of Biomedical and Translational Research Vol.14 No.2
Canine parvovirus (CPV2) is one of the most virulent virus causing acute hemorrhagic enteritis and myocarditis in dogs. Infection mainly caused by the ingestion of virus through the mucosal route. Therefore, induction of mucosal immunity is essential in prevention of Canine Parvovirus (CPV2) infection. For safe and effective delivery of viral antigens to the mucosal immune system, a novel surface antigen display system for lactic acid bacteria using the poly-γ-glutamic acid synthetase A protein (pgsA) of Bacillus subtilis as an anchoring matrix was applied in order to display CPV2 antigen on the surface of the recombinant L. casei. Recombinant fusion proteins comprised of pgsA and the capsid protein (VP2-S1) showed stable expression in Lactobacillus casei. Surface localization of the fusion protein was verified by cellular fractionation analyses. Oral and nasal inoculations of recombinant L. casei into mice resulted in high levels of serum immunoglobulin G (IgG) and mucosal IgA, as demonstrated by ELISA using recombinant VP2-S1 proteins. Mice receiving intranasal immunization mounted higher antibody response than those receiving oral immunization. These results indicate that mucosal immunization with recombinant L. casei expressing CPV2 VP2-S1 protein on its surface provides an effective means for elicitation of strong antibody responses against CPV 2 VP2-S1.
고흥선,Beong Kug Yang,Bae Keun Lee,Kyung Hee Jang,Seong Teak In,Jong Hyek Lee,Kwang Seon Kim,Gu Hee Kweon 충북대학교 동물의학연구소 2012 Journal of Biomedical and Translational Research Vol.13 No.4
To identify subspecies of the sika deer, Cervus nippon, and to select C. n. hortulorum individuals for the restoration of this subspecies in Korea, we obtained cytochrome b partial sequences (375 bp) of sika deer (C. nippon) in deer farms from northeastern China, North Korea, mainland Korea, and Jeju Island, based on noninvasive samples: these cytochrome b sequences were compared to the corresponding haplotypes of sika deer, obtained from GenBank. We identified five individuals of sika deer from deer farms in northeastern China and North Korea as C. n. hortulorum, and found that interbreeding between individuals belonging to different subspecies is common in deer farms from far-eastern Asia. We concluded that the five C. n. hortulorum individuals in deer farms from northeastern China and North Korea, detected in this study, cannot be considered as pure descendants of C. n. hortulorum in the wild.
엄상용,김용대,김헌,홍장수 충북대학교 동물의학연구소 2013 Journal of Biomedical and Translational Research Vol.14 No.2
Apolipoprotein A1 (ApoA1) is the major protein component of high density lipoprotein (HDL) cholesterol in blood, and ApoA1 genetic polymorphisms modulate the blood lipid profiles. This study was conducted in order to investigate the association between three genetic polymorphisms (rs670, rs5069, and rs5070) of ApoA1 and blood lipid profiles in postmenopausal Korean women. A total of 130 postmenopausal women who visited a hospital in order to undergo screening tests were subjects of this study. Genetic polymporphisms and blood lipid profiles were determined using a direct sequencing and spectrophotometric assay, respectively. A significant linkage disequilibrium was observed between all tested single nucleotide polymorphisms. ApoA1 rs5070 genetic polymorphism showed a marginally significant association with HDL cholesterol levels (p=0.066). After adjusting for age, body mass index, smoking, alcohol drinking, medication, hypertension, and diabetes mellitus, we found that the ApoA1 rs5070 genetic polymorphism is a significant determinant of HDL cholesterol levels (β=4.421, p=0.037). According to the results of this study, ApoA1 rs5070 genetic polymorphism may be an important genetic marker associated with HDL cholesterol in postmenopausal Korean women.
Inhibitory Effect of Natural Killer Cells on Liver Tumor Growth in Mouse Xenograft Model
박지성,Hyeran Sung,Il-Hoi Kim,CHONG-KIL LEE,Jin Tae HONG,송석길 충북대학교 동물의학연구소 2012 Journal of Biomedical and Translational Research Vol.13 No.2
Human natural killer (NK) cells are major player in innate immune response. The functions of these cells as a scavenger of cancer cell are enhanced by cytokines such as interleukin-2 (IL-2), which play an important role of immune response on both tumors and virally infected cells. Liver cancer has a high incidence rate and is a major cause of death in Korea. We provide evidence that human NK cells inhibit tumor growth of the hepatocellular carcinoma cell line SNU-354. NK cells were cultured with human IL-2 for 14 days, yielding an enriched NK cell population that contained 35% CD8+cells, 6% CD4+cells, 51% CD16+/CD56+ cells. NK cells at doses from 2.5 to 10 million cells/mouse were intravenously injected once a week in nude mouse model that retains human liver tumor induced by implantation of SNU-354 cells. The results demonstrate that human NK cells were recruited within tumor tissue and inhibited SNU-354 tumor growth by 32%, 58%, and 65%. The present data suggests that NK cell-based immunotherapy may be used for therapy of human liver cancer.
Hematologic and hemodynamic findings during hemodialysis in 13 beagle dogs
지창무,정해원,장효미,문종현,정동인 충북대학교 동물의학연구소 2014 Journal of Biomedical and Translational Research Vol.15 No.2
Hemodialysis is an effective therapy for renal failure in veterinary practice. To evaluate hematologic and hemody- namic changes during hemodialysis, 13 dogs were treated with hemodialysis, after which complete blood cell counts (CBC), serum chemistry, and mean systolic blood pressure were analyzed. For CBC, white blood cells (WBC) and platelets underwent significant changes. In serum chemis- try, there were significant differences in blood urea nitrogen (BUN), creatinine, total protein, albumin, globulin, amylase, calcium, potassium, and phosphorus contents. Further, mean systolic blood pressure suddenly increased in early hemodialysis and decreased significantly thereafter. During hemodialysis, adverse effects were observed in some dogs as follows: bleeding (1 dog), anemia (2 dogs), leukopenia (8 dogs), thrombocytopenia (2 dogs), and hypotension (1 dog). This study demonstrates hematologic and hemodynamic effects during hemodialysis as well as complications similar to human medicine. Before applying the commercialized human hemodialysis system to canine renal failure patients, we monitored hematologic and hemodynamic findings dur- ing hemodialysis in healthy beagle dogs.
홍선화,박상호,이윤성,김옥진 충북대학교 동물의학연구소 2013 Journal of Biomedical and Translational Research Vol.14 No.3
Mycoplasma (M.) hyopneumoniae is the causative agent of swine enzootic pneumonia, a disease that is prevalent in every country where pigs are raised. In this study, we aimed to develop a sensitive and specific PCR assay to detect M. hyopneumoniae in pigs. The suitability of this PCR assay for the detection of mycoplasmal infection was also tested using clinical lung samples from slaughtered pigs. We developed a probe and M. hyopneumoniae-specific primer pairs, MhyoP-F and MhyoP-R, for the new PCR assay based on regions in the Mycoplasma protein P97 gene that are unique to M. hyopneumoniae. The developed PCR assay was very specific and sensitive for the detection of M. hyopneumoniae. The assay was able to detect the equivalent of 10 pg of target template DNA, which indicates that the assay was very sensitive. In addition, the M. hyopneumoniae PCR assay detected only M. hyopneumoniae and no other Mycoplasma spp. or bacterial species of another genera. Further, the newly developed PCR assay effectively detected M. hyopneumoniae infection in pigs. We suggest that this PCR assay using M. hyopneumoniae-specific primer pairs,MhyoP-F and MhyoP-R, will be useful and effective for monitoring M. hyopneumoniae infection in pigs.