Super-resolution Imaging of Complex Biological Systems via Chemical Expansion Microscopy

Jae-Byum Chang(장재범) 한국고분자학회 2021 한국고분자학회 학술대회 연구논문 초록집 Vol.46 No.1

Visualization of the molecular structures inside biological systems is essential to understand the molecular mechanisms of biological phenomena occurring inside these systems. In 2015, a new super-resolution fluorescence imaging technique, called expansion microscopy (ExM), was demonstrated. ExM can achieve sub-60-nm resolution with diffraction-limited microscopy by physically expanding target specimens with a swellable hydrogel. Here, we demonstrate a new ExM technique, which we termed chemical ExM, which can visualize almost all biological structures. In chemical ExM, all biological structures are directly labeled with fluorophores. As the molecular weight of fluorophores is around 1,000 Da, specimens with a thickness of several millimeters can be stained and imaged with a sub-60-nm resolution after expansion. Chemical ExM would be an attractive tool to study various biological systems, as it enables the super-resolution imaging of thick specimens without antibodies.

TFT Backplane Technologies for AMLCD and AMOLED Applications

최재범,장영진,박철호,최범락,김효석,박기찬 한국물리학회 2009 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.54 No.1

We thoroughly investigated low-temperature polycrystalline silicon (LTPS) thin-film transistor (TFT) backplane technologies based on (1) a melt-mediated crystallization process with laser systems, (2) a solid phase crystallization process with advanced annealing systems and (3) a single-crystalline Si layer transferred onto a large glass substrate for at-panel-display applications. Extensive micro-structural analyses of the silicon films, comparison of the TFT performances and evaluation of the image quality of the displays enabled us to choose the competitive technologies for large-area active-matrix liquid-crystal display (AMLCD) and active-matrix organic light-emitting diode (AMOLED) applications. We thoroughly investigated low-temperature polycrystalline silicon (LTPS) thin-film transistor (TFT) backplane technologies based on (1) a melt-mediated crystallization process with laser systems, (2) a solid phase crystallization process with advanced annealing systems and (3) a single-crystalline Si layer transferred onto a large glass substrate for at-panel-display applications. Extensive micro-structural analyses of the silicon films, comparison of the TFT performances and evaluation of the image quality of the displays enabled us to choose the competitive technologies for large-area active-matrix liquid-crystal display (AMLCD) and active-matrix organic light-emitting diode (AMOLED) applications.

2P-210 Fabrication of Chiral Nanostructure by Directed Self-Assembly

최홍균,장재범,( Caroline Ross ) 한국공업화학회 2017 한국공업화학회 연구논문 초록집 Vol.2017 No.1

In this study, we demonstrate that confinement of a cylindricalmorphology BCP in a shallow circular pit can produce either concentric rings or a spiral. A spiral is promoted by the presence of a notch-shaped feature within the template which controls the spiral chirality. The length of the spiral increases with the diameter of the template. Design of the notch geometry enabled double spirals to be formed. A notch of width □ L0 promotes spirals even for commensurate pit sizes indicating the critical importance of the inner shape of the template. For smaller notches, spirals formed for incommensurate template diameters and rings for commensurate template diameters. Analogous to using a notch to initiate a spiral in a circular pit, our approach could be extended to guide the chirality of 3D helical spirals formed in cylindrical confinements with a helical ramp template.

Synthesis of Inorganic Structures Mimicking Specific Protein Assemblies in Multicellular Organisms via Antibody-Guided Biotemplating

송창우,송대현,안재완,김일두,장재범 한국고분자학회 2023 한국고분자학회 학술대회 연구논문 초록집 Vol.48 No.2

Study on the sample disruption process to acquire super-resolution images of various organs via expansion microscopy

김세인,이진아,조유진,조인,장재범 한국고분자학회 2017 한국고분자학회 학술대회 연구논문 초록집 Vol.42 No.1

분무열분해(噴霧熱分解) 공정(工程)에 의한 주석(朱錫) 산화물(酸化物) 나노 분말(粉末) 제조(製造)

유재근,차광용,김명춘,한정수,장재범,이용화,김동희,Yu, Jae-Keun,Cha, Kwang-Yong,Kim, Myung-Choun,Han, Joung-Su,Jang, Jae-Bum,Lee, Yong-Hwa,Kim, Dong-Hee 한국자원리싸이클링학회 2008 資源 리싸이클링 Vol.17 No.6

본 연구는 폐 주석의 리싸이클링을 통한 고기능성 주석 산화물 나노 분말의 대량제조 기술개발을 위한 전 단계 연구로서 주석 염화물 용액을 원료로 하여 분무열분해 반응에 의하여 평균입도 50nm 이하의 주석 산화물 분말을 제조하였으며 반응온도의 변화에 따른 생성 입자들의 특성 변화를 파악하였다. 열분해 반응온도가 $800^{\circ}C$로부터 $850^{\circ}C$로 증가함에 따라 형성된 입자들의 평균입도는 20 nm로부터 30 nm로 증가하였다. 또한 XRD 피크의 강도도 증가하였으며 비표면적은 1/2 정도로 크게 감소하였다. 반응온도 $900^{\circ}C$의 경우에는 액적 형태는 평균입도 30 nm 정도의 나노 입자들로 구성되어 있는 반면 독립된 입자들의 경우에는 평균입도가 $80{\sim}100\;nm$로 현저하게 증가 하였으며 입자 표면이 더욱 치밀화되어 있었다. 또한 XRD 피크 강도도 현저히 증가하였으며 비표면적은 현저하게 감소하였다. 반응온도 $950^{\circ}C$의 경우에는 액적 형태의 비율 및 크기가 현저히 감소하였으며 대부분의 입자들은 독립된 형태를 유지하고 있었으며 평균입도는 약 70 nm로 $900^{\circ}C$의 경우보다 오히려 감소하였다. 또한 XRD 피크의 강도도 $900^{\circ}C$의 경우에 비하여 현저히 감소하였으며 비표면적은 2배 정도 크게 증가하였다. This study is the previous stage for the mass production technology development of the nano-sized tin oxide powder by the recycling of the wasted tin metal, and nano-sized tin oxide powder with the average particle size below 50 nm is prepared from the tin chloride solution by the spray pyrolysis process. As the reaction temperature increases from 800 to 850, the average particle size of the generated powder increases from 20 to 30 nm. As the reaction temperature increases to 900, the droplet type is composed of the particles with the average size of the 30 nm. while the average size of the independent particles increases up to $80{\sim}100$ nm and the surface microstructure becomes more solid. Until $900^{\circ}C$, as the reaction temperature increases, the XRD peak intensity increases, while the specific surface area decreases. When the reaction temperature increases to 950, most of the powder appears with the independent type and the average particle size decrease down to 70 nm. The XRD peak intensity greatly decreases and the specific surface area increases almost twice.

Antidepressant effect of StillenTM

정현자,김정화,김나래,유명숙,남선영,김규업,최영진,장재범,강인철,백남인,김형민 대한약학회 2015 Archives of Pharmacal Research Vol.38 No.6

StillenTM has been used to treat patients withgastric mucosal ulcers and has an anti-inflammatory effect. It is well-known that neuro-inflammatory reactions arerelated to depression. Here we evaluated the antidepressant-like effect of StillenTM on mice subjected to the forcedswimming test (FST). StillenTM and eupatilin (a majorcomponent of StillenTM) significantly decreased immobilitytimes compared with the FST control group. In theStillenTM-administered group, increased levels of5-hydroxytryptamine (serotonin) and brain-derived neurotrophicfactor protein were observed in the hippocampus. Nissl bodies also increased in the hippocampus neuronalcytoplasm of the StillenTM-administered group. StillenTMdecreased levels of interleukin (IL)-1b, IL-6, and tumornecrosis factor-a (at the mRNA and protein levels) in thehippocampus and serum, compared with the control group. In addition, the mRNA expression of estrogen receptor-bincreased after StillenTM administration in the hippocampus. These findings suggest that StillenTM should beviewed as a candidate antidepressant.

Acute and 28-Day Subacute Toxicity Studies of Hexane Extracts of the Roots of Lithospermum erythrorhizon in Sprague-Dawley Rats

한정탁,김명준,문설희,전유림,황재식,남춘자,박종우,이선호,나재범,박찬성,박희원,이정민,장호송,박선희,한경구,최영환,이혜영,강종구 한국독성학회 2015 Toxicological Research Vol.31 No.4

Lithospermum erythrorhizon has long been used as a traditional oriental medicine. In this study, the acute and 28-day subacute oral dose toxicity studies of hexane extracts of the roots of L. erythrorhizon (LEH) were performed in Sprague-Dawley rats. In the acute toxicity study, LEH was administered once orally to 5 male and 5 female rats at dose levels of 500, 1,000, and 2,000 mg/kg. Mortality, clinical signs, and body weight changes were monitored for 14 days. Salivation, soft stool, soiled perineal region, compound-colored stool, chromaturia and a decrease in body weight were observed in the extract-treated groups, and no deaths occurred during the study. Therefore, the approximate lethal dose (ALD) of LEH in male and female rats was higher than 2,000 mg/kg. In the subacute toxicity study, LEH was administered orally to male and female rats for 28 days at dose levels of 25, 100, and 400 mg/kg/day. There was no LEH-related toxic effect in the body weight, food consumption, ophthalmology, hematology, clinical chemistry and organ weights. Compound-colored (black) stool, chromaturia and increased protein, ketone bodies, bilirubin and occult blood in urine were observed in the male and female rats treated with the test substance. In addition, the necropsy revealed dark red discoloration of the kidneys, and the histopathological examination showed presence of red brown pigment or increased hyaline droplets in the renal tubules of the renal cortex. However, there were no test substance-related toxic effects in the hematology and clinical chemistry, and no morphological changes were observed in the histopathological examination of the kidneys. Therefore, it was determined that there was no significant toxicity because the changes observed were caused by the intrinsic color of the test substance. These results suggest that the no-observed-adverseeffect Level (NOAEL) of LEH is greater than 400 mg/kg/day in both sexes.

Conversion of Advanced Materials via Labeled Biostructure (CamBio): A New Approach to Broaden Intracellular Biostructure Templating Methods

송대현,송창우,조승희,권태윤,서준영,김지윤,유재영,이혁모,신현정,정연식,장재범 한국고분자학회 2023 한국고분자학회 학술대회 연구논문 초록집 Vol.48 No.2

부재료 침지처리가 김치의 냄새 및 발효 특성에 미치는 영향

최아름(A Reum Choi),박동일(Dong Il Park),유귀재(Guijae Yoo),김소영(Soyoung Kim),장재범(Jae Bum Jang),채희정(Hee Jeong Chae) 한국식품영양과학회 2009 한국식품영양과학회지 Vol.38 No.11

김치와 각각의 부재료들의 향기 성분을 비교하기 위하여 GC/MS로 정성 분석한 결과, 김치의 향기 성분은 sulfide 계열의 황 화합물과 유기산, 알코올류 등으로 나타났고, 김치의 냄새를 구성하는 물질들은 김치의 냄새를 각각의 부재료에서 검출된 향기 성분들로부터 유래한 것으로 확인되었다. 김치 특유의 이취를 저감시키기 위하여 김치의 부재료인 마늘, 생강, 양파 및 파를 각각 5 ㎜의 길이와 0.3 ㎜의 두께로 세절하고 100℃에서 2분간 데친 후, 정수된 물 4℃에서 12시간 동안 침지처리 하였다. 부재료 침지처리가 김치의 군덕내(moldy odor)와 신내(sour odor)에 미치는 영향을 관능검사로 평가한 결과, 세절침지처리김치에서 일반김치에 비하여 신내와 군덕내가 크게 감소한 것으로 나타났다. 또한 부재료의 침지처리가 숙성 중 김치에서 pH, 산도, 유산균수에 영향을 미치는 것으로 나타났다. The volatile flavor compounds of kimchi and its sub-ingredients were analyzed using GC/MS. The major volatile compounds of kimchi were identified as sulfide compounds, organic acids and alcohols. It was confirmed that the major volatile flavor compounds of kimchi originated from sub-ingredients such as garlic, ginger, onion and reek. To reduce the characteristic odor of kimchi, the sub-ingredients (garlic, ginger, onion and reek) were chopped into a length of 5 ㎜ and a thickness of 0.3 ㎜, blanched at 100℃ for 2 min, and then soaked in water at 4℃ for 12 hr. The effects of soaking of the sub-ingredients on sensory evaluation with regard to characteristic odor of kimchi such as sour and moldy odor were investigated. The sour and moldy odors of kimchi were significantly reduced by the soaking of sub-ingredients. Additionally the addition of soaked sub-ingredients in kimchi had influences on the change of pH, total acidity and lactic acid bacterial count of kimchi during fermentation.