한국인 당뇨병 및 비당뇨병 환자에서의 뇌혈관 질환 유무에 따른 PAI-1 촉진자 유전자형과 인슐린저항성에 관한 연구

오승준,김영설,박철영,김덕윤,김성운,양인명,김진우,최영길,팽정령,정경천 대한비만학회 2000 The Korean journal of obesity Vol.9 No.2

연구배경 : 혈전현상을 특징으로 하는 질환에서는 Plasminogen activator inhibitor-1 (PAI-1) 이 높은 활성도를 보이는데, PAI-1 치는 당뇨병, 심근경색증, 비만 등에서 높다고 밝혀진 바 있다. 또한 당뇨병 환자들의 합병증의 주요한 병인은 죽상경화증으로 혈전현상이 특징인 질환에서 증가하는 PAI-1이 당뇨병 환자에서 높다. 목적 : 정상인에서의 PAI-1 유전자 촉진자의 유전자형의 분포 및 혈액농도를 관찰하고, 당뇨병 및 뇌혈관 질환 환자군에서의 PAI-1 유전자 촉진자 유전자형의 분포 및 혈액농도를 측정하여 정상인과 차이점을 알아본다. 당뇨병 환자군에서의 혈장 PAI-1 치와 인슐린 저항성, 전구 인슐린 등과의 상관관계를 살펴보고, 인슐린저항성과 대혈관질환의 지표로 사용될 수 있는지 알아보았다. 방법 : 대상으로는 정상인 76명, 제2형 당뇨병 환자 56명, 뇌혈관질환이 동반된 제2형 당뇨병 환자 48명, 뇌혈관질환 환자 51명을 선택하여, 환자의 혈액에서 인슐린, 공복시 혈당, 전구인슐린, 중성지방, 총콜레스테롤 및 기타 생화학 검사 및 이학적 검사를 시행하였다. 환자의 DNA를 채취하여 전사개시 -675bp를 포함하는 대립형질 특이 시발체를 사용하여 중합효소 연쇄 반응을 실시하여, 그 유전자형을 판독하였다. 결과 : 정상 대조군 76명 (46.4±11.1세), 2형 당뇨병 환자 56명 (58.3±12.6세), 뇌경색증 환자 51명 (63.1±13.2세) 대상으로 하였다. PAI-1 촉진자 유전자형의 (4G/4G, 4G/5G, 5G/5G)빈도는 정상 대조군이 각각 23.7%, 75.0%, 1.3%, 뇌경색 환자군이 19.6%, 66.7%, 13.7%, 뇌경색이 동반된 당뇨병 환자군이 33.3%, 58.3%, 8.3% 였다. (X2=12.6, p=0.05). 이러한 사실은 서구인에 비해 4G/4G, 5G/5G 동형 유전자형이 낮은 결과였다. 각 군별 혈장 PAI-1 농도는 정상 대조군 13.4, 1.8 ~ 65.2 ng/mL (중앙값 , 범위 ) 2형 당뇨병 환자군 14.4, 2.9 ~ 47.8 ng/mL, 뇌경색 환자군 21.9, 6.2 ~ 154.7 ng/mL , 뇌경색이 동반된 2형 당뇨병 환자군 28.8, 3.2 ~ 139.3 ng/mL 로 차이를 보였다 (p=0.000). 전체 대상에서 PAI-1 촉진자 부위의 유전자형에 따른 PAI-1 활성도와 항원 농도는 차이를 보이지 않았다. 그러나 PAI-1 활성도는 혈중 중성지방, 전구인슐린, 체질량지수와 독립적인 상관관계를 보였다 (p=0.000, p=0.000 and p=0.005). 결론 : 결론적으로 PAI-1 촉진자 부위의 유전자형은 뇌경색증의 지표는 아니며, PAI-1 활성도를 결정짓는 인자는 유전적 요인보다는 혈중 중성지방, 전구 인슐린, 체질량지수와 같은 대사적 요인으로 생각된다. Plasminogen activator inhibitor-1 (PAI-1) is known be related to insulin resistance and several components of the large vascular disease. Notably, the high frequencies of diseases such as coronary heart disease or stroke are related to type 2 diabetes complications. We studied to find out whether the PAI-1 promother genotype could be a marker for cerebral infarction in type 2 patients. Subject patients were; 56 type 2 diabetics (age 58.3±12.6), 51 patients with cerebral infarction (age 63.1±13.2), 48 type 2 diabetics with cerebral infarction (age 64.8±9.3) , and 76 healthy control (age 46.4±11.1). The 4G/5G genotype of PAI-1 promoter was evaluated by polymerase chain reaction and endonuclease digestion. PAI-1 promoter genotype frequency (4G/4G, 4G/5G, 5G/5G) was 23.7%, 75.0% and 1.3% in healthy control, 17.9%, 67.9% and 14.3% in type 2 diabetes patients, 19.6%, 66.7% and 13.7% in cerebral infarction patients, 33.3%, 58.3% and 8.3% in type 2 diabetics with cerebral infarction (X^2=12.6, p=0.05). This finding is lower in frequency of 5G/5G homozygote than that reported in Caucasians. The plasma PAI-1 concentrations according to the disease were 13.4, 1.8 ~ 65.2 ng/mL (median, range) for healthy control, 14.4, 2.9 ~ 47.8 ng/mL for type 2 diabetes, 21.9 6.2 ~ 154.7 ng/mL for cerebral infarction , and 28.8, 3.2 ~ 139.3 ng/mL, for cerebral infarction with type 2 diabetes (p=0.000). In the all subjects, PAI-1 concentration and activity of PAI-1 promoter genotype did not show any significant difference. However, the PAI-1 activity was independently associated with serum triglyceride level, plasma proinsulin and BMI (p=0.000, p=0.000 and p=0.005 respectively). We concluded that PAI-1 genotype is not a marker for the cerebral infarction ; however, the genotype is related to PAI-1 concentration , and therefore it seems to be that metabolic factors such as triglyceride level or plasma proinsulin or BMI are more in relations with determining the PAI-1 concentration than the genotype.

흰쥐에서 중추 고장성식염 주입에 의한 승압효과에 미치는 ANP의 영향

오승호,양민준,염철호 조선대학교 1995 The Medical Journal of Chosun University Vol.20 No.1

The present study was designed to examine the effects of intracerbroventricular (ICV) administration of atrial natriuretic peptide (ANP) on the pressor response induced by ICV hypertonic NaCl in rats. Rats (male. Sprague-Dawley) weighing 220-300g were anesthetized with pentobarbital sodium (50mg/kg. IP) and their right lateral cerebral ventricle were cannulated. Both isotonic and hypertonic NaCl (0.15 M, 0.6 M and 1.2 M) were ICV (3μl/min) applied and arterial blood pressure and heart rate (HR) responses registered. Central administration of hypertonic NaCl solution caused an elevation in mean arterial pressure (MAP) and HR, while the response magnitude was comparable correlated to the NaCl concentration. The ANP (3pmol/min, ICV) alone did not cause recognizable changes in MAP. When ANP was administered with 0.6 M NaCl, however, hypertonic NaCl-induced changes in MAP were interrupted. These results indicate that ANP may have the role in central cardiovascular regulation. It is also suggested that in some conditions. ANP may act as an antipressor substance in the brain.

Plasmid DNA pBR 322의 증폭과 Hydroxyapatite Chromatography에 의한 분리

오두환,유주현,신원철,정건섭,유승구 연세대학교 산업기술연구소 1984 논문집 Vol.16 No.1

The amplification and the isolation conditions of pBR322 plasmid DNA were investigated. For the amplification of pBR322 plasmid DNA, cells from logarithmic growth phase were most effective. The optimal conditions for the amplification of pBR322 plasmid DNA in Escherichia coli GM4 were obtained as follows; 50-150 ㎍/ml of chloramphenicol concentration, 6-8 hours of incubation time in the presence of chloramphenicol. Ampicillin and tetracycline had no effect on the amplification of pBR322 plasmid DNA. Approximately 1 mg of pBR322 plasmid DNA was purified from 3.5 ℓof Escherichia coli GM4 culture broth by hydroxyapatite chromatography. The purified pBR322 plasmid DNA was expressed in Escherichia coli C600.

참나무 亞屬 주요 수종의 물리적 성질에 따른 역학적 성질에 관한 연구(Ⅱ) : 휨강도 Bending strength

吳承源,韓哲洙 全北大學校 1995 論文集 Vol.40 No.-

This study was carried out in order to get some basic data for wood rationally utilization by means of clearing mechanical properties which were lived in korea. Six lepidobalanus such as Quercus variabilis, Q. aliena, Q. serrata, Q. mongolica, Q. acutissima, Q. dentata were used for this research. The summarised results of this study were as following. 1) To the moisture content increased, bending strength and modulus of elasticity were inclined to decrease. 2) To the specific gravity increased, bending strength and modulus of elasticity were inclined to increase.

방광요관 역류환아에서 항역류수술후 99mTc-DMSA 신주사 소견의 분석

오승준,고재준,이경철,임대정,이승배,이안기,김광명,최황 大韓泌尿器科學會 1998 Korean Journal of Urology Vol.39 No.9

알코올 사용 장애 환자의 스트레스와 대처 양식 및 부적응에 관한 연구

박민철,오상우,이귀행,노승호,백영석,이상열,김재현 圓光大學校 醫科學硏究所 1999 圓光醫科學 Vol.15 No.2

Background: This study was explored the influences of perceived stress, coping style on maladaptation of patients with alcohol use disorders. Methods: To investigate these objects, 66 patients with alcohol use disorders were completed scales for the perceived stress, coping style, dysfunctional attitude, alcohol expectancy, self-efficacy, social support, state-trait anxiety, quantity of alcohol use, problems of alcohol use and BDI. The data were analyzed by t-test, correlation and multiple regression analysis. Results: First, the results showed the significant difference between long term group and short term group on the problem-related alcohol use and trait anxiety. Second, the results showed the significant difference between severe problem related alcohol use group and mild problem related alcohol use group on cognitive coping, negative expectancy, quantity of alcohol use. Third, among each variables of patients with alcohol use disorders, quantity of alcohol use had significantly high correlation with quantity of alcohol by family, quantity of alcohol by friends, perceived stress, negative expectancy. Problems of alcohol use had significantly high correlation with negative expectancy, cognitive coping, percieved stress, social support and self efficacy. Finally, vulnerability-stress model predicted 26.1% of variances of quantity of alcohol use, 48.9% of variances of problems of alcohol use and 13.9% variances of depression. Conclusions: The results of the study indicated the superiority of vulnerability stress model to predict quantity of alcohol use, problems of alcohol use and depression.

포도당과 이소프로테레놀에 의한 시상하부의 소마토스타틴 분비가 갑상선자극호르몬방출호르몬에 의한 갑상선자극호르몬에 미치는 효과

박철영,양인명,오승준,김덕윤,우정택,김성운,김진우,김영설,최영길 대한내분비학회 2000 Endocrinology and metabolism Vol.15 No.4·5

악골낭종에 대한 임상적 연구

문철,이동근,민승기,오승환,김태성 대한악안면성형재건외과학회 2000 Maxillofacial Plastic Reconstructive Surgery Vol.22 No.3

This clinical study was made of 214 cases of the jaw cysts which were diagnosed histopathologically in the Department of Oral and Maxillofacial Surgery, Wonkwang University Dental Hospital from Jan. 1, 1991 to Dec. 31, 1998. Medical records, radiographs, histopathological reports of 214 cases with jaw cysts were retrospectively reviewed. The results were as follows : 1.Among the total patient of 214 cases, male were 143 cases, and female are 71 cases, male predominated by the ratio of 2.04. 2.The periapical cysts and the dentigerous cyst are the most common cyst, irrespective of 116 cases(54.2%) and 58 cases(27.1%). 3.The periapical cyst has no gender prediction, male were 70 cases and female were 54 cases, the mean ages were 39.3 years(SD=18.4). The most common involving teeth were maxillary anterior teeth(59.7%) . 4.The dentigerous cyst predominantly occurred in third decade (18 cases : 31%), and the mean ages were 31.9 years(SD= 14.8). Dentigerous cysts predominantly occurred in male by the ratio of 7.29 (male : 51 cases, female : 7 cases). Maxillary anterior teeth(43.1%) and mandibular molars(32.8%) were most frequently involved. 5.The odontogenic keratocyst predominantly occurred in second and third decade and mean ages were 23.5 years(SD= 17.2). The mandibular molars(60%) were most frequently involved. 6.All nasopalatine cyst occurred after fourth decade, mean ages were 49.5years(SD= 10.4). In the gender incidence of nasopalatine cyst, 1 case occurred in female and 10 cases occurred in male. 7.In the surgical intervention of jaw cyst, cyst enucleation and marsupialization performed in 156 cases. Cyst enucleation with bone graft performed in 58 cases and iliac autogenous bone grafts most frequently performed in 29cases. 8.The diameter range of cyst enucleation with bone graft were from 15mm to 120mm with a cyst diameter of 33.3mm. (SD = 15.2). The diameter range of cyst enucleation and marsupialization were from 7mm to 82mm with a cyst diameter of 20.4mm.(SD=9.0) The diameter of cyst enucleation with bone graft were significantly greater than diameter of cyst enucleation. (p<0.05) 9.Post-operative complications occurred in 9 cases(15.5%) in which cyst enucleation with bone grafts performed and occurred in 31 cases(19.9% ) in which cyst enucleation.

圓光醫大 附屬 第2病院 神經精神科 患者에 對한 統計的 考察(Ⅱ)

박민철,장광철,김상원,김한주,유미경,김훈,유용진,김현정,박남진,오상우,이귀행,노승호,백영석 圓光大學校 醫科大學 神經精神科學敎室 1992 圓光精神醫學 Vol.8 No.1

본 연구는 1986년 7월 11일부터 1991년 7월 10일까지 원광의대 부속 제2병원 신경정신과에서 의래 및 입원치료를 받았던 6,072명의 환자를 대상으로 병원 역학조사를 실시하고 그 결과를 1차조사 (박민철 등1986)와 비교했다. 1.전체 환자 중 남자가 54.4%로 여자보다 많았고, 이리와 이리 인접지역이 84.0%로 대부분의 지역을 나타났다. 의료보장상태는 보험환자가 57.1%, 의료보호환자21.8%, 일반환자 21.1%였으며 연령별로는 20대 24.6%, 30대 22.8%로 20대 30대가 절반정도를 차지했다. 2.외래환자에서는 신경중성장애가 46.0%로 가장 많고 정동장애, 경련성장애 순이며, 입원환자에서는 정신분열증이 30.5%로 가장 많고 기질성정신장애, 정동장애의 순이었다. 3.남자는 신경증성쟝애(23.4%), 정신분열증, 기질성정신장애 순이고 여자는 신경증성장애(39.9%), 정동장애, 정신분열증의 순이었다. 남자가 여자보다 많은 진단은 알코올정신장애(94.6%), 기질성정신장애, 소아정신장애 순이고, 여자가 남자보다 많은 진단은 정동장애(64.5%), 신경증성장애였다. 4.진단별 연령분포에서 신경증성장에는 30대(25.3%), 20대, 40대 순이고, 정신분열증은 20대(40.5%), 30대, 40대 순이며, 정동장애는 20대, 30대, 50대 순이나 비교적 비슷한 융이었다. 5.진단별 발병연령에서 신경증성장에는 20대, 30대가 절반이고,정신분열증은 20대(51.3%), 10대, 30대 순이며 정동장애는 20대(30.7%), 30대, 50대 순이었다. 진단별 평균 발병연령은 신경증성장애 32.7세, 정동장애 36.1세, 정신분열증 23.6세였다. 6.진단별 이병기간은 2년이상이 신경증성장에 28.3%, 정신분열증 68.5% 정동장애 43.9%인데 전체적으로 41.0%였다. 진단별 평균 이병기간은 정신분열증 73.2개월, 정동장애 45.5개월, 신경증성장애 29.5개월이었다. We examined epidemiologic study of 6,072 patients who visited Wonkwang Neuropsychiatric hospital since July 11 of 1986 until July 10 of 1991 and compared this results with previous study(Park et al 1986). The study revealed as follows:1. Of total patients males visited this hosptial more than females as 57.1%, Most of them(84.0%) resided Iri and neighbouring Iri. In the point of insurance, insured patients, medicaid patients and uninsured patients were 57.1%, 21.8% and 21.1% respectively. On the distribution of age, the twenties, the thirties were 24.6%, 22.8% respectively and twenties and thirties were about half of total patients. 2. Of outpatients neurotic disorder was the most as 46.0%, secondly mood disorder and thirdly convulsive disorder, but of inpatients schizophrenia was the most as 30.5%, secondly organic mental disorder and thirdly mood disorder. 3. Of males neurotic disorder was the most as 23.4%, secondly schizophrenia, and thirdly organic mental disorder, but of females neurotic disorder was the most as 39.9%, secondly mood disorder and thirdly schizophrenia. alcoholic mental disorder(94.6%), organic mental disorder, child mental disorder were mostly found in males,but mood disorder(64.5%) and neurotic disorder were mostly found in females. 4. On the distribution of age neurotic disorder was found the most in the thirties(25.3%), next in the twenties and in the forties, and schizophrenia was found the most in the twenties(40.5%), next in the thirties and in the forties, but mood disorder was found the most in the twenties, next in the thirties and in the forties but revealed similar rates relatively. 5. On the distribution of age of onset neurotic disorder developed nearly half during the twrnties and the thirties, schizophrenia developed mostly during the twenties (51.3%), secondly the teens and thirdly the thirties, but mood disorder developed firstly the twenties(30.7%), secondly the thirties and thirdly the fifties. on the average age of onset of illness neurotic disorder, mood disorder and schizophrenia were 32.7 years, 36.1 years and 23.6 years respectively. 6. On the duration of illness more than two years was 28.3% in neurotic disorder, 68.5% in schizophrenia and 3.9% in mood disorder and on the average duration of illness schizophrenia, mood disorder and neurotic disorder were 73.2 months, 45.5 months and 29.5 months respectively.

피질골 절제술을 응용한 구치의 원심직립 이동

김상철,강경화,오승환,이인성,김선영 대한치과교정학회 2004 대한치과교정학회지 Vol.34 No.5

빠르며 정확하고 안전한 치아이동을 목표로 삼고 있는 교정치료에서 새로운 패러다임이라고 여겨지는 피질골 절제술과 견인 골형성술을 응용한 구치의 원심직립이동에 대하여 알아보았다. 일반적인 방법으로는 어렵거나 치료 기간이 길어질 것으로 판단되는 구치의 원심직립 이동을 피질골 절제술과 견인 골형성술을 응용하여 도모하였다. 각각의 원심직립 증례를 통해 피질골 절제술과 견인 골형성술의 적용 술식, 견인장치 등을 논하고 그 효과를 파악하였다. 빠른 치아이동과 이에 따른 교정치료 기간의 단축이 가능하고 통상의 교정치료로는 이동에 한계가 있었던 치아이동을 구치의 정출이나 고정원의 상실 없이 도모할 수 있었다. Tooth movement facilitated by corticotomy and distraction osteogenesis, a new paradigm in orthodontics, was discussed. Molar uprighting was thought to be either difficult or nearly impossible. In this study, a section of cortical bone. which may act as resistance to tooth movement in alveolar bone, was removed. Active bone deposition was also made possible in the tension side. This forms the main concept of tooth movement facilitated by corticotomy and distraction osteogenesis. Molars in two cases were uprighted at such a speedy rate as we could not Imagine in conventional tooth movement, which lead to reduction of the total treatment period. And molar uprighting was possible without side effects, for example, supraversion of the tooth or anterior anchorage loss. These were the superior aspects to conventional orthodontics.