Cucurbita pepo 에서 획득한 light membrane vesicle로 부터 ATP-hydrolysis 단백질의 분리

오승은 建國大學校基礎科學硏究所 1992 理學論集 Vol.17 No.-

호박 하배축으로부터 4/6%(w/v) Ficoll gradient를 사용해서 light membrane vesicle를 분리하고 Triton X 100을 사용하여 vesicle에 결합된 단백질들을 용해한 후 Sepharose CL-6B ion-ex-change chromatography를 사용해서 단백질을 분리하고 300~500 mM NaCI elution 분획들을 모았다. 다시 Sephadex G 100으로 gel-filtration하여 두개의 ATP-hydrolysis 단백질을 분리하였다. 그중 한 단백질이 사람의 적혈구 원형질 막의 Ca²??-ATPase에 대해서 형성된 다크론성 항체와 교차 반응을 일으켰다. Light membrane vesicles were isolated from the zucchini hypocotyl by 4/6%(w/v)Ficoll gradient and the proteins were solubilized with Triton X 100. The solubilized protins were seperated with Sepharose CL 6B ion-exchange chromatography and the proteins which were eluted at 300~500 mM NaCI were collected. From these collected fractions two ATP-hydrolysing proteins were seperated by Sephadex G-100 gel-filtration. One of ATP-hydrolysing proteins was crossreacted with polyclonal antibodies against Ca²?? -ATPase of human erythrocyte.

뇌경색을 동반한 Haemophilus parainfluenzae에 의한 감염성 심내막염 1예

오은영,배상수,정윤재,이창근,이혁,김성민,박승우,백경란,송재훈 대한화학요법학회 1998 대한화학요법학회지 Vol.16 No.3

저자들은 뇌경색을 동반한 H. parainfluenzae에 의한 심내마염을 ampicillin과 gentamicin의 병합요법으로 치료한 1예를 경험하였기에, 이를 문헌고찰과 함께 보고하는 바이다. We experienced a case of infective endocarditis due to Haemophilus parainfluenzae complicated by cerebral infarction. A 40 year-old woman was admitted because of right-sided hemiparesis and dysarthria, which was accompanied by high fever up to 40 C with chills. She had been suffering from low-grade fever and general weakness for one month prior to admission. Blood cultures grew H. parainfluenzae, and the brain CT scan demonstrated cerebral infarction of MCA territory. Transesophageal echocardiogram showed mitral valve prolapse with regurgitation, and no vagetation was found. She was successfully treated with ampicillin and gentamicin for six weeks. To our knowledge, this is the first case of infective endocarditis caused by H. parainfluenzae in Korea.

분광 앨립소미트리를 이용한 유리기판 위의 박막 분석

성덕경,방경윤,오영륙,최은호,안일신,김옥경 한양대학교 이학기술연구소 2003 이학기술연구지 Vol.6 No.-

Spectroscopic ellipsometry 은 다양한 물질의 광학적 특징을 연구할 수 있는 강력한 기술이다. 현재 실리콘 웨이퍼 위의 박막 분석에는 그 효율성이 확실히 입증되어 있다. 그러나 현재 산업은 유리 기판위의 박막을 분석하기를 원하고 있다. Ellipsometry로 투명기판을 분석하기 위해서는 후면 반사에의한 비간섭성 효과를 고려해야 한다. 기존의 반사공식을 투명기판의 비간섭성을 고려하여 수정하였다. 투명기판 위의 샘플과 scratch를 낸 같은 샘플을 측정 분석하여 정확성을 확인하였다. 수정된 반사공식을 이용하면 디스플레이와 같은 투명재료를 이용하는 곳에 유용하게 사용될 것으로 기대되고 있다. A Spectroscpic ellipsometry is very strong technology to study optical properties of many materials. It has already substantiated a analytic efficiency about thin films above wafers to be made of silicon. However, the current industry needs thin films analyzed on a glass. When we analyze a glass using a ellipsometry, we have to consider a efficiency of incoherence due to reflect at the back side. So then, we slightly altered the original formula. We compared a scratched sample with a sample on the glass, because off getting a exactitude. Our technical development, using the changed formula, is expected the useful utilization in a part of using gIasses such as display.

거주자 의식을 고려한 중층 고밀도 집합주택의 계획에 관한 연구

오승은,이영수 대한건축학회 2003 대한건축학회 학술발표대회 논문집 - 계획계/구조계 Vol.23 No.1(계획계)

The study is an attempt to explore user's preference of design elements for the Medium-height and High density collective Housings Planning. The purpose of the research is to suggest design guidelines for the collective Housings Planning applicating high-rise multi purpose housings' advantages. The analysis of the user's preference is resulted in necessary of considering activity and union senses in housings. Therefore, that housing can be planned with convenience facilities for the dwellers and circulation plan purpose for feeling in intimacy and amenity. The commercial and public space planning which behavior overlap and go through become to feel active in the housings.

NDRG2 is one of novel intrinsic factors for regulation of lL-10 production in human myeloid cell

최승철,김광동,김종태,오상석,윤선영,송은영,이희구,조영경,최인표,임종석,김재화 Sookmyung Women's University Research Institute of 2010 여성과 건강 Vol.6 No.1

당뇨병과 동반된 화농성 간농양의 임상상 및 예후인자

오은숙,강무일,이원영,오기원,임동준,이소영,이정민,고승현,김성래,안유배,손현식,윤건호,차봉연,이광우,손호영,강성구 대한당뇨병학회 2000 임상당뇨병 Vol.1 No.1

연구배경: 감염질환은 혈관질환과 함께 당뇨병의 중요한 사망원인의 하나이며, 혈당조절이 불량할수록 감염 빈도가 증가됨은 잘 알려져 있다. 간농양의 발생이 있어서도 당뇨병은 중요한 기저질환 중의 하나이다. 일반적으로 간농양의 가장 흔한 원인균은 Escherichi coli라고 알려져 있으나, 최근 당뇨병을 가진 간농양 환자에서 Klesiella pneumoniae의 검출이 증가되고 있다. 또 최근 연구에 의하면 K.pneumonie가 간농양의 가장 흔한 원인균이며, K. pneumoniae에 의한 간농양은 K.pneumoniae 이외의 균주에 의한 간농양보다 당뇨병과 밀접히 관련되어 있다고 보고된 바 있다. 이에 저자들은 간농양 환자를 대상으로 당뇨병환자의 빈도를 알아보고, 그 원인균 및 특징을 비당뇨병 환자들의 경우와 비교해 보고자 하였다. 방법: 1992년 1월부터 1999년 6월까지 방사선학적 검사 혹은 수술에 의해 간농양이 확인되거나, 경피적 천자나 수술을 통한 배농액 배양, 혹은 혈액 검사에서 원인균이 증명된 182명의 환자를 대상으로 후향적 임상고찰을 실시하였다. 결과: 화농성 농양을 가진 167명의 환자(남:94명, 여:73명)중 당뇨병을 가진 환자는 54명 (남:30명, 여:24명)으로 32.3%를 차지하였다. 환자의 평균나이는 당뇨군에서 62.1 ± 13.4세, 비당뇨군에서는 55.1 ±15.5세로 당뇨군에서 높았으며(p=0.0021), 남녀간의 평균나이 비교 시 두 군 모두 여성의 나이가 의미있게 높았다. 비당뇨군에 비하여 당뇨군의 경우 저알부민혈증의 빈도와 aspartate transaminase가 증가되어있었다. 원인균주는 K. pneumoniae (당뇨군54%, 비당뇨군 39.1%), E. coli(당뇨군 17.5%, 비당뇨군 18.8%), Pseudomonas aeruginosa, Streptococcus viridans, Enterococcus 순이었으며 두 군간의 의미는 차이는 없었으나 K. pneumoniae의 경우 다른 균종에 비해 단독감염의 빈도가 높았다(82.8%). 사망률은 당뇨군에서 16.7%, 비당뇨군에서 7.1%로 당뇨군에서 의미있게 높았으며(p=0.019), 당뇨군에서의 사망환자는 모두 패혈증이 동반되어 있었다. 당뇨환자의 사망군은 생존군에 비해 당뇨병 이환기간이 길고 혼합감염의 빈도가 높았다(p=0.046). 검사실 소견에서는 총 빌리루빈 및 알카리성 포스파타제의 증가가 관찰되어 있었다. 결론: E. coli가 간농양의 주된 원인균이라는 기존 보고와는 달리 당뇨군과 비당뇨군 모두 K. pneumoniae가 간농양의 주된 원인균이었다. 또한 당뇨 유병기간이 길고 당뇨조절이 불량한 환자에서 혼합감염이며 총 빌리루빈 및 알카리성 포스파타제의 증가등과 같은 위험인자가 존재할 경우 사망률이 증가될 수 있으므로 이들 군에서는 적극적인 치료가 필요할 것으로 사료된다. Background: Liver abscesses are commonly associated with underlying disease, particularly diabete mellitus. The number of the liver abscesses caused by Klebslella pneumoniae in diabetic patient has been increased in Korea nowadays. This study was conducted to clarify the clinical presentation and prognostic factors of pyogenic liver abscesses, especially in diabetic patients and to determine the proportion of K. pneunomiae as a pathogen in liver abscess in Korea. Methods: Medical records of 167 patients treated for pyogenic liver abscess from January, 1992 through June, 1999 were reviewed retrospectively in detail. Major Pathogenic organism, clinical manifestations, prognostic factors, the importance of diabetes mellilus as an underlying disease and its effect on clinical features and prognosis were analyzed. Results: Among 167 cases of pyogenic liver abscess, underlying diabetes mellitus was present in 32.3%. The mean age of patients was 62.7 13.4 years in diabetic liver abscess group and 55.1 15.5 years in non-diabetic liver abscess group. Most liver abscesses were cryptogenic in origin or secondary to the billary tree diseases. The clinical presentations among the two groups were not significantly different. When compared to patients without diabetes, patients with diabetes had significantly higher proportions of hypoalbuminemia and elevated aspartate transaminase level. The most common organism of the pyogenic abscess was K. pneumoniae in both groups. Percutaneous drainage of the abscess with appropriate antibiotics was the most commonly used therapeutic modality in liver abscess. The mortality rate of diabetic liver abscess is 16.1 % and 7.1% in nondiabetic liver abscess. Complications, especially septicemla, were found more frequently in patients with diabetes than in patients without diabetes (64.8% vs 28.3%, septicemia : 31.5% vs 9.7%). The factors influencing mortality in the diabetic liver abscess were disease duration, mixed infection, presence of septicemia, elevated bilirubin and alkaline phosphatase. Conclusions: In contrast to prior report that the E. coli was the most common pathogen in liver abscess, we found that K. pneumoniae was the most common organism cultured in liver abscess. Diabetic patients have more complications and higher mortality than patients without diabetes. Early detection and proper treatment are needed to improve the outcome for diabetic patient with liver abscess.

기관골 신생증 1예

오영상,이은우,정종혁,문승원,김수현,김동한,양혁승,박정환,박경옥,강성수,이영직 朝鮮大學校 附設 醫學硏究所 2006 The Medical Journal of Chosun University Vol.31 No.2

Tracheobronchopathia osteoplastica is a rare disorder of unknown cause characterized by the projection of numerous bony or cartilaginous submucosal nodules into the tracheobronchial lumen with sparing of the posterior membranous portion of tracheobronchial tree. In the past, most of the cases were diagnosed as secondary finding at autopsy. Nowaday, the key component of the evaluation is flexible fiberoptic laryngoscopy and bronchoscopy, which typically demonstrates irregular spicules of subrnucosal bone and cartilage projecting into the tracheobronchial lumen and causing various degrees of airway obstruction. A 45 years old man was visited Sunchon St. Carollo hospital due to discomfort on throat. We saw several nodules just below vocal cord via laryngoscope. The computed tomography revealed multiple small calcified nqdules on trachea and both main bronchi. Pathologic finding of bronchoscopic biopsy showed abnormal proliferation of bony and cartilagious nodules in the tracheal submucosa. These fingdings were consistent with tracheobronchopathia osteoplastica. We report here on a case of tracheobronchopathia osteoplastica with review of literature.

모 제약회사 근로자들의 직업성 천식 유병률과 약제 분말 노출수준

오성수,최용휴,김은아,이영목,장재길,유장진,박승현,김태균,정수영,김규상,강성규 大韓産業醫學會 2006 대한직업환경의학회지 Vol.18 No.2

목적: 제약회사에서 직업상 천식의 유병 규모를 파악하고 작업공정에 대한 작업환경 측정을 통해 제약회사의 약제 분말에 대한 노출수준을 평가하고자 하였다. 방법: 제약회사에서 약제 분말에 노출되는 근로자를 대상으로 천식을 선별하기 위하여 설문조사 및 진찰, 혈액검사, 폐기능 검사 그리고 피부단자시험을 실시하였고, 각 부서별 공정에 따라서 총분진과 항생제 또는 효소제 분말에 대한 작업환경 측정을 실시하였다. 직업상 천식이 의심되는 근로자에 대하여 기관지과민성에 대한 검사(메타콜린을 이용한 비특이 기관지유발시험)와 약제 분말 기관지유발시험을 실시하여 확진하였다. 결과: 설문조사 및 진찰에서 작업 관련 천식 증상이 있는 근로자는 11명(34.4%)이었고, 약제 분말을 이용한 피부단자시험에서 1+ 이상 반응을 보인 근로자 10명(31.2%)이었다. 비특이 기관지유발시험 결과 8명(25.0%)이 양성(PC20 ≤16 mg/ml)이었다. 약제 분말 기관지유발시험을 통해 직업상 천식으로 확진된 근로자는 5명(15.6%)이었다. 작업환경 측정에서 원료를 충진하거나 투입하는 작업 그리고 정립작업에서 약제 분말에 대한 노출이 많았다. 결론: 제약회사에서 직업상 천식은 주로 제조공정 중 약제 분말에 노출된 근로자에서 발생하여, 증상 및 직업성 천식의 유병률이 높았다. 따라서 원료를 충진 또는 투입하는 작업과 정립작업 등 제조과적에서 약제 분말 노출을 줄이기 위한 세심한 주의가 필요하여, 약제 분말을 취급하는 근로자에 대해서는 천식 증상 조사뿐 아니라 정기적으로 피부단자시험, 비특이 기관지유발시험 등을 실시하여야 할 것이다. Objectives: After the investigation of one worker with occupational asthma, we surveyed the preva- lence of occupational asthma and the exposure level of pharmaceutical dust of 32 workers in a pharmaceutical company. Methods: Thirty-two of the 90 employees participated in the survey which consisted of questionnaire, blood sampling, spirometry and skin prick tests with 8 common allergens as well as 9 antibiotics and 2 enzymes. Various indices of the working environment were also measured. Subjects who had a symptom suggestive of work-related asthma or positive skin prick test were further investigated by PC20 methacholine. Nine subjects who had a PC20 result of 16 mg/ml or less (n=7) or had work-related symptoms and positive skin prick test (n=2) were referred to undergo a specific bronchial provocation test to phar- maceutical dust in an academic allergic disease center. Results: Eleven of 32 workers (34.4%) had a work-related symptom suggestive of occupational asthma. Ten (31.2%) showed positive skin prick test. 8 (25.0%) had a PC20 result of 16 mg/ml or less (indicative of significant bronchial hyperresponsiveness), and 5 (15.6%) had a positive result on the specific bronchial provocation test. Exposure levels of stuffing, input of raw materials and screening process were relatively high. Conclusions: This survey showed that pharmaceutical workers have an increased risk of occupational asthma. Although pharmaceutical factories maintain a relatively good working environment, careful control of respiratory tract exposures, especially during stuffing, input or raw materials and screening process, is important to prevent occupational asthma. Pharmaceutical workers need to undergo regular skin prick and methacholine bronchial provocation tests, as well as asthmatic symptom survey, to ensure the early detection and prevention of occupational asthma.

NDRG2 is one of novel intrinsic factors for regulation of lL-10 production in human myeloid cell

최승철,김광동,김종태,오상석,윤선영,송은영,이희구,조영경,최인표,임종석,김재화 Sookmyung Women's University Research Institute of 2010 여성과 건강 Vol.6 No.1

유고사회주의 연방과 여성의 지위 변화

오승은 부산외국어대학교 지중해연구소 2003 지중해지역연구 Vol.5 No.2

The present research mainly examines how women's position had changed or had not changed in the course of socialist modernization. The research argues that the socialist modernization implemented by the Yugoslav communists represented both an opportunity for and an obstacle to addressing women's issue in Yugoslavia. The Communist leaders brought about a lot of changes and, to a considerable extent, they enabled traditionally house-bound women to make their way into society. Despite this seemingly-great achievement, the paper holds that the authoritarian nature of the communist rule stopped the communist leaders from truly addressing the women's issue. It concludes that the authoritarian leadership maintained or even enhanced the traditionally strong patriarchal value inherent in the society. With little changes made in the value system dominating the society, no reform was able to reach its logical conclusion. For this reason, the socialist modernization failed to liberate women from the traditional society. It was often said that Communism was a nightmare for men while it was a paradise for women. This saying may be true in some senses. Many indications show that the female members of the Yugoslav society benefited from drastic social changes and legal protection. For example, the 1946 Socialist Constitution proclaimed the equality of women in terms of employment education and marriage. The legal protective devices were put into practice. By the 1970s, women's illiteracy rate dropped to 10% from 57% in the 1940s. Women's employment rate rose 7 times higher in the mid 70s than in the 40s. Yet, these statistics fail to convey the whole picture of women's issue in Yugoslavia. These changes favorable to women happened only outside their home. They may have gained social, economic and political freedom much more than ever before. Yet, not much had changed inside home. Women may have found themselves working shoulder to shoulder with their male co-workers at work place. Yet back at home, they had to serve for male members of the family as their grandmothers and mothers did. The reason can be found in the patriarchal value that still dominated the society. Authoritarianism inherent in the communist system failed to weaken it. The communist leaders, who were authoritarian themselves, shared the need to liberate women only to the extent that they needed female laborers to achieve the socialist revolution women. Most of them who were male-chauvinistic head of the family themselves, did not see the need to liberate women as an individual member of the society. As a result, women were made to endure the double burden of working both at home and at work and remained as minority of their society. The unresolved issue of women's discrimination raised its ugly head again in the post-communist Yugoslavia. With most of the legal protection removed, women found themselves even more discriminated in the post-socialist society. Thus, the socialist modernization hold mixed meanings and draw mixed reactions from the female members of former Yugoslavia.