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파슬리 4CL1 유전자의 cis - acting element 에 결합하는 DNA 결합 단백질의 특성파악
오승은 한국유전학회 2000 Genes & Genomics Vol.22 No.4
For the purification of transcription factor (s) binding to a cis-acting element footprint 56 (FP56) that regulates the developmental expression of parsley 4CL1 gene, the binding properties of synthetic oliganucleotide O56 including the FP56 sequence to nuclear proteins was determined. According to the electrophoretic mobility shift assay (EMSA) using O56, four major DNA-protein complexes from tobacco vegetative tissues and cultured cells were separated and named as tobacco binding proteins (TBPs). Among the major DNA-protein complexes, TBP2 and TBP3 were the specific binding products and the binding activities of both TBPs were dynamically varied depending on the growth phase of cultured cells. These two DNA-binding proteins bound to O56 independently even in 30 sec after the start of incubation for the EMSA. Interestingly, the binding activities of both proteins were promoted by treatment with alkaline phosphatase. The effect of phosphatase was suppressed by the treatment of NaF, a phosphatase inhibitor. Through the affinity chromatography coupled to O56 multimers, TBP3 eluted in 1M KCI while TBP2 eluted in 0.6, 0.8 and 1M KCI elution.
오승은,김순례,Oh, Seung-Eun,Kim, Soon-Lae 한국가정간호학회 2007 가정간호학회지 Vol.14 No.2
Purpose: This thesis focuses on researching the burden of the Home Health Care that the Family Care-givers have. Method: This study had been conducted for the duration of 3 consecutive months from January 2006 to March 2006 and had investigated 120 person registered as the Home Health Care Clients to a University Hospital located in Incheon City. The thesis adopted the 'feeling of burden' measuring method jointly developed by Seo, Mi-Hye and Oh, Ga-Sil(1993), and FACES-III(Family Adaptability Cohesion Evaluation) developed by Olson(1985) etc. translated by Kim, Yun-Hee(1989) as the measuring method for Family Function. Result: The overall Health Status of the Home Health Care Clients was 2.18 point of average out of 3 point, where as 1.83 for Activities of Daily living and 1.98 for Vital sign, which are below the average. In the mean time, the Competence for Modified Barthel Index marked 30.88 point out of the full mark. The analyzed result of the burden that the family members have was 3.43 point of average out of 5 point which shows that the interviewee feel that they are considerable burden to their families. When it comes to 'the Burden to the family members' and 'the Function of the family' according to the characteristics of the patient, the result shows statistically significant differences, which are varied according to gender, the relationship between the care-givers and the patients. When it comes to the Family Cohesion, the difference was examined as 'statistically significant' according to the Academic background of the patients and the relationship between the patients and the interviewee.(p<0.05). The result also suggests that there exists 'Negative correlation' among the level of patients' health status, the Family Cohesion and the Family Burden. Conclusion: From the result of this study stated above, this thesis is strongly insisting that there is an urgent need for us to develop a health care mediation program, which could eventually reduce the burden of home health care that the patients' family have. At the same time, a follow-up research to prove the effect of the program is imminent.
오승은 부산외국어대학교 지중해연구소 2003 지중해지역연구 Vol.5 No.2
The present research mainly examines how women's position had changed or had not changed in the course of socialist modernization. The research argues that the socialist modernization implemented by the Yugoslav communists represented both an opportunity for and an obstacle to addressing women's issue in Yugoslavia. The Communist leaders brought about a lot of changes and, to a considerable extent, they enabled traditionally house-bound women to make their way into society. Despite this seemingly-great achievement, the paper holds that the authoritarian nature of the communist rule stopped the communist leaders from truly addressing the women's issue. It concludes that the authoritarian leadership maintained or even enhanced the traditionally strong patriarchal value inherent in the society. With little changes made in the value system dominating the society, no reform was able to reach its logical conclusion. For this reason, the socialist modernization failed to liberate women from the traditional society. It was often said that Communism was a nightmare for men while it was a paradise for women. This saying may be true in some senses. Many indications show that the female members of the Yugoslav society benefited from drastic social changes and legal protection. For example, the 1946 Socialist Constitution proclaimed the equality of women in terms of employment education and marriage. The legal protective devices were put into practice. By the 1970s, women's illiteracy rate dropped to 10% from 57% in the 1940s. Women's employment rate rose 7 times higher in the mid 70s than in the 40s. Yet, these statistics fail to convey the whole picture of women's issue in Yugoslavia. These changes favorable to women happened only outside their home. They may have gained social, economic and political freedom much more than ever before. Yet, not much had changed inside home. Women may have found themselves working shoulder to shoulder with their male co-workers at work place. Yet back at home, they had to serve for male members of the family as their grandmothers and mothers did. The reason can be found in the patriarchal value that still dominated the society. Authoritarianism inherent in the communist system failed to weaken it. The communist leaders, who were authoritarian themselves, shared the need to liberate women only to the extent that they needed female laborers to achieve the socialist revolution women. Most of them who were male-chauvinistic head of the family themselves, did not see the need to liberate women as an individual member of the society. As a result, women were made to endure the double burden of working both at home and at work and remained as minority of their society. The unresolved issue of women's discrimination raised its ugly head again in the post-communist Yugoslavia. With most of the legal protection removed, women found themselves even more discriminated in the post-socialist society. Thus, the socialist modernization hold mixed meanings and draw mixed reactions from the female members of former Yugoslavia.
Cucurbita pepo 에서 획득한 light membrane vesicle로 부터 ATP-hydrolysis 단백질의 분리
오승은 建國大學校基礎科學硏究所 1992 理學論集 Vol.17 No.-
호박 하배축으로부터 4/6%(w/v) Ficoll gradient를 사용해서 light membrane vesicle를 분리하고 Triton X 100을 사용하여 vesicle에 결합된 단백질들을 용해한 후 Sepharose CL-6B ion-ex-change chromatography를 사용해서 단백질을 분리하고 300~500 mM NaCI elution 분획들을 모았다. 다시 Sephadex G 100으로 gel-filtration하여 두개의 ATP-hydrolysis 단백질을 분리하였다. 그중 한 단백질이 사람의 적혈구 원형질 막의 Ca²??-ATPase에 대해서 형성된 다크론성 항체와 교차 반응을 일으켰다. Light membrane vesicles were isolated from the zucchini hypocotyl by 4/6%(w/v)Ficoll gradient and the proteins were solubilized with Triton X 100. The solubilized protins were seperated with Sepharose CL 6B ion-exchange chromatography and the proteins which were eluted at 300~500 mM NaCI were collected. From these collected fractions two ATP-hydrolysing proteins were seperated by Sephadex G-100 gel-filtration. One of ATP-hydrolysing proteins was crossreacted with polyclonal antibodies against Ca²?? -ATPase of human erythrocyte.