후두암의 보존적 수술에 대한 임상적 고찰

정경호,정상술,홍기환 釜算慶南耳鼻咽喉科學會 1994 임상이비인후과 Vol.5 No.1

결핵균 30 kDa 항원과 Triton X-100 Solubilized Protein 항원에 의한 대장암 주변 림프절 단핵구의 활성화

박정규,김광호,조은경,임재현,민들레,송영자,김화중,백태현 忠南大學校 癌共同硏究所 1998 癌共同硏究所 硏究誌 Vol.2 No.1

Tumor-draining lymph node mononuclear (TDLMN) cells are specifically sensitized to the growing tumor but such cells are deficient for mediating an antitumor response. In this study, we examined the feasibility of using mycobacterial 30 kDa or Triton X-100 solubilized protein (TSP) antigen to stimulate mononuclear cells of colon cancer-draining lymph node for the generation of cell mediated immune effector cells. The proliferative response of TDLMN cells stimulated with mycobacterial 30 kDa or TSP antigen was determined by ^(3)H-thymidine incorporation assay. The proliferation of TDLMN cells to mycobacterial 30 kDa or TSP antigen was significantly increased in PPD (+) patients, but a poor response to the 30 kDa or TSP antigen was observed in PPD (-). The expression on γδ T cells to mycobacterial 30 kDa or TSP antigen was assessed by flow cytometry. The γδ T cells from PPD ( + ) patient responded only to 30 kDa antigen but to TSP antigen. An investigation of cytokine mRNA expression was undertaken using reverse transcription-polymerase chain reaction (RT-PCR) to follow TDLMN cells stimulated with the 30 kDa or TSP antigens for 5 days. The IFN-γ and TNF-α mRNA expression was only induced in TDLMN cells of PPD ( + ) patient in response to the 30 kDa or TSP antigen. The IL-2 mRNA expression was induced in both PPD (+) and PPD (-) in response to the 30 kDa or TSP antigen. But the IL-4 mRNA expression was not induced in response to the 30 kDa or TSP antigen. These results suggest that the 30 kDa and TSP antigens may serve as biologic response modifier for the generation of cell mediated immune effector cells.

選手들의 不安과 運動行動의 關聯性에 關한 硏究

김정완,최경호 慶尙大學校 1999 論文集 Vol.38 No.-

The purpose of this study was to explore the adaptability of the Spielberger's 'state-trait anxiety test' for measuring athlete's state and trait anxiety. Questionnaire in-cluding 40 items for anxiety, 5 items for motivation, 13 items for self-efficacy, 6 items for performance was administrated to subjects. The subjects of this survey were 277 male and 124 female athletes of middle school(n=24), high school(n=364), and college(n=13) in Kyeong Sang Nam Do. The results of this study were as follows: 1) Accord-ing the result of factor analysis for items to measure, 3 factors(psychological safety, trait anxiety, and state anxiety)were extracted. 2) The validity(Cronbach's alpha) of each factors was .829 for psychological safety, .773 for trait anxiety, and 776 for state anxiety. 3) By the results of Multiple regression, Forward step-wised multiple regression, and t-test be-tween high and low group of sports anxiety score, The common and differential mechan-isms between Trait Anxiety and State Anxiety were founded. Both Trait Anxiety and State Anxiety statistically significant predict Psychological Safety. While Trait anxiety influenced to self-efficacy, motivation, and performance, State Anxiety didn't. The major limit of this study was a restricted sample. Thus, a future cross-validation study using another nationalized sample should be followed.

문화관광축제의 평가속성에 관한 연구

박호표,이경숙,서정수 한국관광정책학회 2003 觀光政策學硏究 Vol.9 No.1

This research has studied what sorts of attributes are prioritized and which of them are important, starting with its purpose that the evaluation of cultural tourist festivals should be executed objectively, systematically and comprehensively. To accomplish the research objective, the analysis of weighted values and the verification of the divergences among groups have been carried out through the attributes able to evaluate cultural tourist festivals output from a preceding study. The research set up two factors in the attributes of evaluation: holders, visitors and detailed attributes of each factor have been observed. First, the areas of holders' evaluation among the attributes by each factor for measurement are the degrees of objectives accomplished, the efficiency of events as wholes, economical efficiency and democratic elements. Second, those of visitors' evaluation are experience activities, facilities, service and approachability. The research has also verified the priorities in each attribute, the degrees of its importance and the divergences among groups by researching the opinions of professionals on the attributes in evaluating from 81 valid samples, a satisfiable outcome has been attained as the whole reliance of holders, local residents, visitors and participating companies was 0.9 plus. In the priority of each factor, the factors for holders have been prioritized economical efficiency, the efficiency of events, the degrees of objectives accomplished and democratic elements in order. Those for visitors have been prioritized service, experience activities, facilities and approachability. Despite the significance of this research that an objective and comprehensive study of cultural tourist festivals has been carried out in it, it maintains some limitations as follows: First, however evaluation should include all of its elements, such as objects, periods, time, evaluators, compensational systems, etc., this research has dealt only with the selection of attributes and the decision of priorities. Hence, another research that considers all of the elements should be carried out. Second, in spite of the fact that evaluation should be not only for the past but also the establishment of long-term strategies and vision, this research lacks discussions on those elements. In other words, the attributes in it has not been selected through vision or strategies, but through a bibliographic study as a form of being verified the relationship between the academic circle and the official-dom. The researches hereafter should be to set up strategies and to come up with vision. Third, this research has failed to compare the thoughts of the involved industry on the attributes in evaluating the festivals since it has limited its respondents only to the two groups: the academic and the official people. If the future researches are carried out in the level of overcoming the limitations indicated above, it is thought that more meaningful results will be obtained.

도산지수를 이용한 부실예측에 관한 연구

심갑용,정호섭,조경선 전북대학교 산업경제연구소 1999 전북대학교 산업경제연구소 논문집 Vol.30 No.-

어떤이 일이 일어나기 전에 그것을 예측하는 것은 그것이 어떤 일이든지 그리 간단하지는 않다. 특히 지진예측은 불가능에 가까울 정도로 어려운 일이라고들 한다. 그에 비해 도산을 사전에 예측하는 것은 훨씬 쉽다. 왜냐하면 지진은 지구의 지각구조가 갑자기 일으키는 현상으로서 그 구조가 충분히 해명되어 있지 않은데 비해, 기업의 도산은 사람이 기업활동을 통해 일어나는 결과로서 그 메커니즘을 상당히 정확하게 파악할 수 있기 때문이다. 기업의 도산은 개인행동이나 기업행동으로서 감지 할 수 있으며 소문이나 기업의 분위기도 기업의 부실이나 도산을 예측하는 중요한 판단 자료일 수 있다. 그러나 이러한 인지보다는 기업이 제공하는 재무자료를 통해서 그 기업의 경영상태나 재무상태를 알 수 있기 때문에 재무정보를 바탕으로 신뢰성있는 기업의 부실이나 도산을 예측할 수 있다. 본 연구에서 도산지수라는 새로운 개념을 도입하여 기존의 연구들이 가지고 있는 기업부실예측의 한계점에 극복하고자 한다. 이를 위해서 본 연구에서는 기존의 연구들이 사용하는 재무비율변수를 그대로 사용하지 않고 기업의 부실이나 도산의 예측에 좀 더 적합하도록 하기 위해 수정한 재무비율변수를 사용할 것이다. 따라서 기존연구에서 사용된 재무비율과 상당히 다르다. 이러한 재무변수로 통계적 기법을 사용하여 부실기업과 비부실기업간의 차이 분석을 통해 결과를 도출하고 이렇게 도출된 변수로 도산지수라는 개념을 사용하여 기업의 부실을 쉽고 용이하게 예측하고자 했다. 그 결과 연구가설에서 설정한 5가지 재무변수를 검증한 결과 부실기업과 비부실기업간의 차이가 매우 유의적으로 나타났으며, 또한 부실전 3년간의 부실기업의 도산지수가 1.0보다 상당히 작게 나타났으며 비부실기업의 도산지수는 1.0보다 크지는 않지만 1.0에 매우 근접해 있었다. 이러한 결과를 바탕으로 도산지수를 이용하여 부실기업을 예측하는데 일반화 가능성을 확인 할 수 있었다. 본 연구에서는 사례연구도 병행하여 그 적용을 타진해보았다.

석청(Mad-honey) 복용 후 유발된 부정맥 1예

김용철,김남호,김승환,최준호,박은미,이상재,이은미,유남진,윤경호,오석규,정진원 圓光大學校 醫科學硏究所 2008 圓光醫科學 Vol.23 No.2

석청에 포함된 grayanotoxin에 의해 독성 작용이 나올 수 있으며, 이러한 독성 작용은 일반적으로 24시간 이내 저절로 회복된다. 최근에 본 저자들은 석청 복용 후 발생한 심실빈맥을 경험하였기에 보고하는 바이다. 49세 남자가 호흡곤란으로 내원하였으며, 내원당시 분당 40회 정도의 동성 서맥이 관찰되었고, 수액을 투여하던 중 심실 빈맥이 발생하였다. 항부정맥제를 투여 후 정상 동율동으로 전환되었으며, 특별한 이상 없이 4일 후 퇴원하였다. Mad-honey intoxication caused by the consumption of honey producted from the nectar of rhododendrons. The grayanotoxins cause the intoxication. The toxic effects of mad-honey poisoning are rarely fatal and generally last for no more than 24 hours. We experienced one case, a 49 years-old man who presented with dyspnea after ingestion of mad-honey. He showed marked sinus bradycardia with < 40 beats per minute on admission. The cardiac rhythm was changed to ventricular tachycardia immediately. These features resolved completely in 24 hours with continuous infusion of amiodarone(600 mg per day) and fluids. We report the case of intoxication of mad-honey as a presentation of fatal cardiac arrhythmia.

중소도시 하수관거의 침입수량 및 수질오염 원단위 산정

김옥형,박승기,정재훈,임경호 公州大學校 工學硏究院 生産技術硏究所 2004 論文集 Vol.12 No.-

The volume of wastewater in combined sewers in study areas usually exceeds capacities of the sewer system or the treatment plant during storms. For this reason, combined sewer systems are designed to overflow occasionally and discharge excess waste water. The 6 monitoring sites were selected from two small cities near the Hongsung prefecture during over a rainy season. Monitoring was performed by collecting grab samples and by measuring the rainfall and flow rates during dry and wet seasons. Generally the flow rate of wastewater in combined sewers was rapidly decreased after 23:00 PM and gradually increased from 06:30 AM in all sites during the dry season. Due to monitoring and statistical analysis, the groundwater contributes on sewage volume increase (average 3.4-25.4% more) during experimental periods. Therefore, this research is focus on the investigation of the effects of stormwater and groundwater to combined sewer systems.

항 Vancomycin 내성균성 항생물질을 생산하는 Streptomyces sp.AMLK-335의 배양조건

이기형,임대석,최경희,이경호,김창한,김정환 건국대학교 동물자원연구센터 2000 動物資源硏究誌 Vol.21 No.-

본 연구는 새로운 항 vancomycin 내성균성 항생물질을 개발하기 위하여 실시되었으며, 전국 각지의 토양 시료로부터 분리한 방선균 중 vancomycin 내성균주인 K-98 Enterococcus faecium 642, K-98 Enterococcus faecium 637 균주에 대하여 항생물질을 생산하는 분리균주 AMLK-335를 선정하여 이 균주의 항생물질 생산에 대한 최적 발효 조건을 검토한 결과, 탄소원 첨가로는 glycerol(0.5%), 질소원 첨가로는 yeast extract(0.5%)가 가장 효과적으로 항생물질 생산에 이용되었고, 비타민을 첨가에 따른 항생물질 생산성의 차이는 크게 나타나지 않았으나, calcium pantothenate 첨가시 항균활성이 비교적 높아졌으며, 금속이온의 첨가는 K+, Pb+, Ba²+, NA²+ , Ca²+를 첨가하였을 때는 항생물질의 생산성이 비교적 높아졌으며, 그중 Ca²+ 첨가구가 가장 생산성이 높았으며, Fe²+ , Hg+의 금속이온을 첨가하였을 때는 항생물질 생산성이 감소하였으며, CaCO₃첨가는 비첨가구보다 높은 항생물질 생산성을 보였으나, 첨가량에 따른 영향은 대체적으로 없었다. pH의 변화에 따른 생산성은 전반적으로 pH가 항생 물질 생산에 영향을 미치지 못하였으나, pH 4.5∼6.0 부근에서는 비교적 높은 항생물질 생산성을보였으며, 통기량에 따른 항생물질 생산성은 통기량이 많을수록 항생물질 생산이 좋았다. 한편 배양기간에 따라 항생물질 생산성을 조사한 결과, 5일째에 가장 높은 항생물질 생산성을 나타내었으며, pH의 변화는 배양 초기에 하강하였다가 점차 상승하는 경향을 보였다. This study was carried out for examining antibiotic productivity of the strain Streptomyces sp. AMLK-335 producing anti-VRE antibiotic. According to different condition, the strain was cultured for 4 days at 27℃ and determined cell growth, pH value and antibiotic productivity using disk diffusion method. In the experiment, the strain Streptomyces sp. AMLK-335 was examined various culture conditions which was added 8 carbon sources, 12 nitrogen sources, 8 vitamins and 11 metal ions in each time and controlled concentration of CaCO₃, initial pH and aeration. From the studies of conditions for antibiotic production, antibiotic productivity was the best to use 0.5% glycerol as carbon source and 0.5% yeast extract as nitrogen source. When several vitamins were added to medium, there was little difference in antibiotic productivity. But the addition of calcium pantothenate slightly increased antimicrobial activity. The addition of metal ion, such as K+, Pb+, Ba²+, NA²+ and Ca²+ slightly increased antibiotic productivity except Fe²+ and Hg+. The addition of CaCo₃showed higher than non-addition experiment in antibiotic production, but there wasn't generally influenced at addition concentration. According to pH change, antibiotic productivity was increased at pH 4.5∼6.0, and when there was a lot of aeration, antibiotic productivity was increased.

독성물질의 세포사 기전 및 세포사 유발물질의 검색법 개발에 관한 연구(Ⅰ) : 독성물질로 인한 파킨슨병 모델에서의 세포사 기전 연구 Study on the cell-death mechanisms of toxin-induced parkinsonism

강태석,김종민,서경원,김영옥,김준규,오재호,이윤동,김규봉,오정자,송연정,임종준,전범석,문전옥,최광식 식품의약품안전청 2000 식품의약품안전청 연보 Vol.4 No.-

MPTP 독성물질이 도파민성 신경세포에 선택적으로 작용하여 산화성 손상에 의한 신경세포사를 일으키는 것을 이용하여 파킨슨병의 동물모델을 만들고, 이를 통해서 아폼토시스를 비롯한 포사의 기전에 대한 연구 및 너코틴의 신경세포 보호효과 여부를 판정하는 실험을 병행하고자 하였다. 파킨슨꾐의 동물모델을 MPTf 독성 물질을 이용하여 확립하였으며, MPTP(30mgag, i.p.)를 투여한 후 1, 2,3, 4, 5일째 흑질 조직을 채춰하여 tarm로 박걸하여 tyrosine hydroxylase 면역조직화학염색을 수행하여 cell countif우한 결과, control은 57.635ce11s, 1일째 친.OfDells,2일째 57.9±6cells,3일릴 없.3±죠ells, 4일째 49.0츠3cells, 5일째 39.4±Scells료 4, 3일째 뚜렷한 신경세포 수의 감소를 보였다. 신경세포사 기전 규명을 위한 아폼토시스 분걱에서는 벼PTP 투여 후 1, 2, 3, 4, 5일째 조직을 채취하여 Hoechst staining, TUNEL staining을 수곡하였는데 양성 반응을 보인 신경세포는 관찰되지 않아. 아폼토시스로 인한 세포사가 관찰되지 않았다. bIPTP 파킨슨병 동물모델에서 nicotine 보호효과 탐색에 관한 실험은 nicat푸e 0.2mgAg을 5일 퐁안 투여 후 리『fP(30mgag)를 CS7Bt/6 마은스에 복강 내주사로 nicotine과 병용 투여한 후 1, 2, 3, 4, 5일째 뇌를 적출하땄다. 신경세포사가 뚜렷이 관찰되기 시작하는 4, 5일째의 신경세포 수의 감소 정도를 20. 30% 정도 약화시키는 경향을 보였으나, nicotine 보호효과에 대한 추가 실헝이 현재 수행 중에 있다. The cause of Parkinson's disease (PD) is largely unknown. However, free radical toxicit? may plaf a role ip. the degeneration of substantia nigra, which is the Hajorfocus of pathological damages in PD. Recently, a neuroprotective effect of nicotine in PD has been suggested. Therefore, the mechanism of neurodegenerafion and protective potential o( nicotine in PD were investigated in the experimental modeB of Pll using a neurotoxin, C57BL/6mice were administered with 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP, 30 mg/kg,j.p.). The degree of neurodegenerafion was determined by immunohistochemical stainiHB oftyrosine hydroxylase (TH). TH-positive cells on nigral sections were found 56.0 ±4, 57.9 ±6,52.315ce11s, 49.0±3cells, and 39,4±Scells at days 1, 2, 3, 4, 5, respectively (controls : 57.6±Scells). Hoechst and TUNEL staining showed no evidence of apoptosis. The exandnation on themice co-adrunistered with nicotine(0.2mgAg) and MPTP(30mgag) revealed a tendency ofnicotine protective effects. At days 4 and 5, the degree of TH-positive cells was decreased by20-30%, In corclusiffn, the role of apoptosis was not evidenced in this MPTP modeB of PB.The possible proteccon by nicotine should be elucidated with further studies.

여호와의 증인 환자에서 산과적 출혈 후 무수혈 치료 2예

김재령,여소진,이해혁,김정식,김태희,남계현,이권해,이임순,박진화,황경호 순천향의학연구소 2004 Journal of Soonchunhyang Medical Science Vol.10 No.2

Jehovah's Witness comprise a unique obstetric population. Their refusal of blood stems from an interpretation of a literal translation of the Bible, and it is this belief that puts them at an increased risk of morbidity and mortality if hemorrhage occurs. We report two cases of a Jehovah's Witness who bled massively due to obstetric hemorrhage, refused blood transfusion and had profound anemia. The patients were treated with recombinant human erythropoietin, parenteral iron and oxygen. And they were treated on an intensive care unit with intermittent positive pressure ventilation. We reviewed with literature considering the therapy for acutely anemic patients who refuse transfusion to decrease the duration of the most severe anemia.