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CA125를 생산하는 자궁내막선암 세포주의 수립과 동정 및 Dibutyl Cyclic Adenosine Monophosphate 에 의한 CA125 생산의 변화에 대한 연구
남계현,이권해,조태호,김순덕,이순곤 대한산부인과학회 1994 Obstetrics & Gynecology Science Vol.37 No.4
1992년 4월 15일 수술을 시행한 68세의 자궁내막선암 환자의 적출 표본으로 부터 새로운 자궁내막선암 세포주 $quot;SCHE-1$quot;을 수립하였다. 첫째, 세포는 16개월이상 접촉저지를 일으키는 일없이 잘 쌓이는 경향을 유지하면서 안정된 증식을 하고 있다. 둘째, 염색체는 이수성으로 분포하고 있으며, 이배체의 것이 가장 높은 빈도를 보였으며, 핵형분석에서도 이상을 보였다. 셋째, 형태학적으로 각각의 세포는 큰핵, 염색질과다(hyperchromatism), 다핵성, 뚜렷한 핵소체 등의 세포학적인 악성기준을 가지고 있었다. 넷째, 전자 현미경소견에서 핵에는 뚜렷한 함요와 여러개의 핵소체가 있으며, 세포사이에는 세포간교가 나타나 세포가 상피성 유래라고 생각되어졌다. 또 다수의 미소융모가 관찰되었다. 또한 SCHE-1세포는 종양 표지물질로 CA125를 생산하고 있었는데 이는 자궁내막암의 기초적인 연구에 유용할 것으로 여겨진다. 또 dbc AMP가 CA125생산에 미치는 영향을 살펴본 결과 자궁내막선암 세포주 SCHE-1는 dbc AMP에 의하여 세포의 증식이 억제되었고, CA125의 생산이 증가되었으며 dbc AMP의 농도가 증가될수록 CA125의 양도 증가되었으나 정상세포는 dbc AMP를 첨가한 후에도 CA125가 생산되지 않았다. A new human endometrial carcinoma cell line, designated SCHE-1, was established from the endometrial adenocarcinoma of a 68-year-old woman. This cell line has grown well for 16 months and has been subcultured more than 50 times. Monolayer cultured cells are polygonal in shape, showing a pavement-lide arrangement and a piling up tendency without contact inhibition. The chromosomal number shows aneuploidy and the modal chromosomal number is in the diploid range. 1*10^5 SCHE-1 cells produced CA 125 (184-682U) during 19 days in cluture media. CA 125 was demonstrated immunohistocytochemically in the original tumor, and SCHE-1 cells. The cells contain no estrogen or progesterone receptors. We studied the effects of dibutyl cyclic adenosine monophosphate on celluiar growth and production of Ca125 in vitro. It is possible that the serum in the in vitro culture medium contains CA 125 and influences the activity of dibutyl cyclic adenosine monophosphate. Dibutyl cyclic adenosine monophosphate (which is said to have an influence upon gene expression) suppressed their proliferation and increased CA 125 levels. A dose-response relationship exists between the concentration of dbc AMP and CA 125 levels in the conditioned media, while the normal cells could not produce even after being administered dibutyl cyclic adenosine monophosphate. The above fact suggests that CA 125 gene expression was induced by dibutyl cyclic adenosine monophosphate.
융모성성선자극호르몬 측정시 단주항체를 이용한 효소면역측정과 방사면역측정의 비교
남계현,조태호,차상헌,이민철 대한산부인과학회 1990 Obstetrics & Gynecology Science Vol.33 No.12
1988년 1월부터 1988년 8월까지 서울 순천향대학병원에 내원한 환자 중 융모성성선자극호르몬측정이 필요한 82개의 혈액가검물에서 융모막성선자극호르몬 다주항체를 이용한 hCG의 효소면역측정값을 구하였고, 또한 방사면역측정을 동시에 실시하여 다음과 같은 결론을 얻었다. 1. RIA에 의한 -hCG값이 5-5,000mIU/ml일 때 ELISA검사치와 조사시 Y=1.74X-111(r=0.969)였다(이하 모두 Y=ELISA의 hCG, X=RIA의 -hCG값). 2. RIA에 의한 -hCG값이 5-200,000mIU/ml일 때는 Y=1.09X-787(r=0.961)였다. 3. 자궁외임신시는 Y=0.804X+927(r=0.989)였다. 4. 임신성융모질환시는 Y=1.12X-1117(r=0.964)였다. 5. 정상임신시는 Y=0.9X+739(r=0.988)였다. hCG의 단주항체를 이용한 효소면역측정법으로 융모성성선자극호르몬을 측정하였고, 동시에 융모성성선자극호르몬을 방사면역측정법으로 측정한 후 유의한 직선의 상관관계가 있음을 확인하고, 융모성 성선자극호르몬 측정시 방사면역측정법 대신에 효소면역측정법을 이용할 수 있다는 가능성을 시사한다. Human chorionic gonadotropin(hCG) is synthesized by trophoblastic tissue and has been established as a reliable index of trophoblastic activity. Since 1927 when Ascheim and Zondek discorved the earliest pregnancy test by bioassay from female rats, diverse methods for assay of the hCG have developed. Among the methods, radioimmunoassay is excellent and accurate but has the disadvantages like as the expences of regents and potential health risks from isotope. We examined the hCG by the RIA and ELISA from several cases of 82 patient simultaneously. The purpose of this study was to evaluate the correlation between RIA and ELISA of hCG and to consider the ELISA as the substitute of RIA of hCG assay. The results were as follows: 1. When the data of RIA were 5-5,000mIU/ml, the regression equation was Y=1.74X-111(Y=data from ELISA, X=data from RIA). The coefficient of corrlation(r) was 0.969. 2. When the data were 5-200,000mIU/ml, the regression equation was Y=1.09X-787. The coefficient of correlation(r) was 0.961. 3. In cases of ectopic pregnancies, the regression equation was Y=1.12X+927. The coefficient of correlation(r) was 0.987. 4. In cases of trophoblastic diseases, the regression equation was Y=1.12X-1117. The coefficient of correlation(r) was 0.964. 5. In cases of normal pregnancies, the regression equation was Y=0.90X+739. The coefficient of correlation(r) was 0.988. We concluded that there were significant positive relationships between RIA and ELISA of hCG assay. So, we considered the possibility of the ELISA as the substitute of RIA of hCG assay in the future.
남계현,이권해,조태호,이임순,고선용,안창훈 대한산부인과학회 1992 Obstetrics & Gynecology Science Vol.35 No.2
최근 본원 산부인과에서 산전 초음파검사 결과 태아복수와 석회화, 음낭 수종 등의 소견이 있었던 태변복막염 환자를 제왕절개 수술로 분만후 신생아는 2차에 걸친 장문합 수술로 현재 특이 사항없이 성장하고 있는 태아 복막염 1예를 경험하였기에, 문헌고찰과 함께 보고하는 바이다. Meconium peritonitis is rare and has been defined as a chemical inflammation of the peritoneum caused by the escape of sterile meconium into the peritoneal cavity of the fetus or newly born infant, resulting from the prenatal small bowel perforation associated with or without obstructive lesions and malformation. Prenatal ultrasonographic detection of the meconium peritonitis allows the preparation for the proper management which should be decreased the mortality and morbidity of the neonate. Recently, a case of prenatal meconium peritonitis which was diagnosed by ultrasonography at 40 gestational weeks in a 40 years old multipara was experienced at our department. We present this case with a brief review of literature.
남계현,이권해,조태호,최용귀,문원실,이치훈,홍기언 대한산부인과학회 1992 Obstetrics & Gynecology Science Vol.35 No.1
필자 등은 48세된 폐경전 여성에게 발생된 혈증테스토스테론치 증가 및 남성화를 동반한 난포막세포 증식증 1례를 경험하였기에 간단한 문헌고찰과 함게 보고하는 바이다. Defeminization & masculinization is a pathologic state in women manifested by androgen excess. Especially, hypertestosteronism in woman has been used as an ovarian marker of androgen exxcess, due to relatively small direct contribution of adrenal secretion. Mainly, testosterone was excreted by theca cells of ovary under the influence of LH(luteizing hormone). We found a case of premenopausal woman with virilizing symptoms & signs associated with the elavated serum testosterone level, and confirmed the hyperthecosis associated with luteinization & cortical stromal hyperplasia of ovaries by surgical pathologic examination. Thus, we present this case with the postoperative follow-up & with the brief review of the concerned literatures.