서울의 Penicillinase Producing Neisseria gonorrhoeae 발생빈도(1998)

김재홍,김준호,반재용,이정우,황성주,정준규,정성태,강진문,조흔정,홍창의,정혜신,이한승,김이선,이봉길,이종호,선영우,한기덕,윤성필,이성훈,안종성,박석범,문승현,조항래,김형섭,류지호,황재영,박준홍,손상욱 한양대학교 의과대학 2001 한양의대 학술지 Vol.21 No.1

In recent years, gonorrhea has been pandemic and remains one of the most common STDs in the world, especially in developing countries. For the detection of a more effective therapeutic regimen and assessing the prevalence of Penicillinase Producing Neisseria gonorrhoeae(PPNG), we have been trying to study the patients who have visited the Venereal Disease Clinic of Choong-Ku Public Health Center in Seoul since 1980 by menas of the chromogenic cephalosporin method. In 1998, 93 strians of N. genorrhoeae were isolated, among which 60(64.5%) were PPNG. The prevalence of PPNG in Seoul, which had been decreased to 39% in 1996 after a peak of 74.3% in 1993, is increased to 64.5% in 1998.

鮟鱇網 漁具 漁法의 改良에 관한 硏究(Ⅱ) : 展開帆을 이용한 改良漁具 試驗(豫報)

李吉來,宋之浩,朴鍾洙,徐萬錫,趙鳳坤,朴正鎬,洪聖根,鄭公炘,裵錫悌,鄭與基,金鎭乾 군산대학교 1980 群山水産專門大學 硏究報告 Vol.14 No.1

Ordinarily stow net fisheries was used by floater beam and sinker beam for the purpose of spreading the mouth of net. But it was not only iuconvenient and dangerous but much defective. In this thesis, we have studied fundermental experiment of the improved stow net fishing gear using head rope, ground rope and otter sail instead of floater beam and sinker beam. From the result of this experiment, it has been succeeded for fishing work to achieve basically without any difficulty. The results obtained in this study were as follows. 1) The resistance of fishing gear was estimated R≒7V2 as possible as it is taken greatly. (R: Resistance V: the rate of current) 2) The interval of otter sail was spreaded smoothly. Being in normal condition, it was maintamed the width 37-38m. It was heared comparison with net mouth of ordinary gear, and the shearing force was enough. 3) The height of mouth was lower than we have anticipated to and seemed to have the supplement of buoyancy. But according to increasing the rate of current, the lowness of mouth height was little affected. 4) The height of otter sail was decreased at the rate of 30% by the rate of current, on the contrary, the height of wing was not decreased owing to sufficient buoyancy, 5) The adjustment of extention length of hanging wire(connected with fishing boat) and raising wire(with otter sail) was great important problem and it must be dealed with the most moderatly because of a corelation between the spreading of fishing gear and catching ability. 6) On the depth of 30m, it was taken 25 minuits to cast anchor and lines, 20 min cast net, 24 min haul net, 45 min haul lines and heave anchor, respectively. 7) The improved stow net with otter sail was cleared up the defects and difficult problems in ordinary gear using floater beam and sinker beam, and it have more advantages of fishing work than ordinary one. But there were involved in lots of problems in details to solve in the future.

일차배양된 설치류 호흡기 상피세포로부터의 점액소 분비에 대한 수종 약물의 영향

이충재,석정호,이재흔,허강민,박지선,배소현,노삼길,박상철 충남대학교 의학연구소 2003 충남의대잡지 Vol.30 No.2

1. PKC activator인 PMA는 일차배양 HTSE세포로부터의 뮤신분비를 0.1μM 농도에서 30%, 1μM 농도에서 80% 가량 증가시켰다. 2. 식물 유래 성분으로, flavonoid의 일종인 TFR은 일치배양 HTSE 세포로부터의 뮤신분비를 10μM 농도에서 50%, 100μM 농도에서 80% 가량 증가시켰다. 3. 양이온성 폴리펩티드인 PLL 및 PLA는 일차 배양 HTSE 세포로부터의 뮤신분비를 0.01 - 10μM 농도에서 용량의존적으로 감소시켰다. 4. 결론적으로, 본 연구에서 얻어진 결과들은 새로운 거담제 및 점액용해제나 단백분해 효소제가 아닌 호흡기 류신의 생성/분비를 조절해 줄 수 있는 신개념의 약물을 개발함에 있어 극히 일부분이나마 단서를 제공하고 있다고 하루 수 있을 것이다. In the present study, we tried to investigate whether phorbol myristate acetate(PMA), trihydroxymethoxy-flavanone rutinoside(TFR) and cationic polypeptides significantly affect mucin release(secretion) from cultured hamster tracheal surface epithelial cells. Confluent primary hamster tracheal surface epithelial (HTSE) cells were metabolically radiolabeled with 3H-glucosamine for 24 hr and chased for 30 min in the presence of each agent to assess the effect on 3H-mucin release. The results were as follows : (1) Both PMA and TFR significantly increased mucin release from cultured HTSE cells ; (2) Cationic polypeptides including po1y-L-lysine(PLL, mw 7,500) and poly-L-arginine(PLA, mw 10,800) significantly inhibited mucin release from cultured HTSE cells, in a dose-dependent manner. This finding suggests us that PMA and TFR be further studied for the possible use as mild expectorants and cationic polypeptides might function as a regulator for hyper-secretion of mucus, both by direct acting on airway mucin-secreting cells, during the treatment of chronic airway diseases.

C6 glia 세포에서 유도성 Nitric Oxide Synthase 유전자 발현조절에 관한 연구

배진영,허강민,배소현,박지선,이충재,이재흔,석정호 충남대학교 의과대학 의학연구소 2003 충남의대잡지 Vol.30 No.1

To investigate transcriptional regulation of iNOS gene by LPS and cytokines, the production of NO, expression of iNOS mRNA and protein, binding activity of nuclear factor-kappa B(NF-kB), and promoter activity of iNOS gene were examined in rat C6 glial cells. LPS, interferon-gamma(IFN-γ), and tumor necrosis factor-alpha (TNF-α) stimulated the production of NO, which was increased synergistically by co-treatment. By the treatment of LPS, iNOS mRNA expression was initiated at 1 h, markedly increased by 3 h, and decreased gradually afterward. iNOS mRNA expression was markedly enhanced by mixture of LPS, IFN-γ and TNF-α. iNOS protein synthesis was increased by the treatment of mixture LPS and cytokine mixture. Treatment of LPS stimulated NF-kB activation, and the activation reached to the maximum level at 30 min, and the treatment of mixture of LPS and cytokines increased the activation. To determine the effect of NF-kB binding activity on iNOS promoter activation, CAT assay was performed. iNOS promoter activity was increased by the treatment with LPS for 5.5 h, and further increased by the combined treatment with LPS and cytokines. These results suggest that NF-kB activation by LPS and cytokines may play a significant role in the induction of the iNOS gene.

골격근 근형질세망의 ATPase활성도에 대한 Vanillylnonanamide의 영향

박경섭,홍장희,류영수,성지연,허강민,임종호,이재흔,석정호 충남대학교 의과대학 지역사회의학연구소 1999 충남의대잡지 Vol.26 No.2

To investigate the effect of vanillylnonanamide(VN) on the ATPase activity of the sarcoplasmic reticulum(SR) of the skeletal muscle, we prepared the SR vesicles from the back muscle of the rabbit, and measured ATPase activity. The results as follows: Ca-ATPase activity was about 50% in the total ATPase activity of skeletal muscular SR. In the reaction mixture with calcium, 100μM VN increased ATPase activity to 20%, and 100 nM and 10 μM thapsigargin(THP) inhibited the ATPase activity to 50% and 60%, respectively. And 100 μM VN plus 100 nM or 10 μM THP more inhibited ATPase activity than THP alone did it. However, without calcium, 100μM VN did not affect ATPase activity, and 10 μM THP inhibited it to 41%, but VN plus THP inhibited it to 31%. The above results show that VN in the reaction mixture with or without calcium has the different action to ATPase activity when it is used alone or together with Ca-ATPase inhibitor THP. This suggests that VN might increase or decrease the skeletal SR Ca-ATPase activity through affecting the lipid membrane around the Ca-ATPase.

Effects of Exercise and a High-Fat, High-Sucrose Restriction Diet on Metabolic Indicators, Nr4a3, and Mitochondria-Associated Protein Expression in the Gastrocnemius Muscles of Mice with Diet-Induced Obesity

Ji-Heun Lee,Didi Zhang,Seong Eun Kwak,Hyung Eun Shin,송욱 대한비만학회 2021 The Korean journal of obesity Vol.30 No.1

Background: Exercise and high fat, high sucrose restriction diets are well known treatments for obesity. The aim of this study was to measure the effects of those lifestyle interventions on molecular transducers of exercise, such as Nr4a3, mitochondria-associated proteins, and muscle function. Methods: We conducted 8 weeks of treadmill exercise and sucrose or fat restriction diets in obese mice. The mice were divided into eight groups: the normal diet (CON) group, normal diet with exercise (CONEX) group, high fat, high sucrose diet (HFHS) group, HFHS with exercise (HFHSEX) group, sucrose restriction (SR) group, SR with exercise (SREX) group, high fat, high sucrose restriction (ND) group, and ND with exercise (NDEX) group. Results: The 8 weeks of exercise reduced body weight, improved lipid profiles (total cholesterol, triglycerides), and increased hanging time. The combination of exercise and a fat and sucrose restriction diet improved glucose tolerance and increased grip strength. The 8 weeks of intervention did not significantly affect the Nr4a3 protein level. The sucrose and fat restriction diet increased the phosphorylated protein kinase B (pAkt)/Akt ratio, and its level was lower in the HFHS group. Exercise increased the protein expression level of PGC-1α in obese conditions. Moreover, SR led reduced the phosphorylated AMP-activated protein kinase (pAMPK)/AMPK ratio and PGC-1α to the control level. Conclusion: The 8 weeks of exercise or a sucrose and fat restriction diet improved metabolic indicators and muscle function. SR reduced pAMPK/AMPK and PGC-1α to the control level. Nr4a3 protein expression was not significantly changed by either exercise or a fat and sucrose restriction diet.

Neuroprotective Effect of Platycarya Strobilacea Infructescence Extract on Transient Focal Cerebral Ischemia in mice

Ji Hye Lee,Ji Heun Jeong,Jong Hun An,Ji Hyun Moon,Seong Hee Kang,Eun Soo Do,Do-Kyung Kim,Nam-Seob Lee,Young-Gil Jeong,Seung Yun Han 대한체질인류학회 2018 대한체질인류학회 학술대회 연제 초록 Vol.61 No.-

FCP 5 : Neocollagenesis and neoelastogenesis after grid fractional radiofrequency treatment; a study in micropig

( Heun Joo Lee ),( Jung In Kim ),( Jung Min ),( Hyun Min Seo ),( Sang Hyeon Hwang ),( Yoon Hwan Kim ),( Joon Hong Min ),( Joon Lee ),( Seung Jae Lee ),( Ji Hye Park ),( Ga Young Lee ),( Soo Hong Park 대한피부과학회 2013 대한피부과학회 학술발표대회집 Vol.65 No.2

Background: To reduce the signs of aging, many different laser and other light-based systems have been developed and evaluated for their ability. Objectives: This study was designed to evaluate the effects of the novel grid fractional radiofrequency (GFR) treatment on skin structure and collagen production in vivo. Methods: The GFR device was used to deliver radiofrequency (RF) energy to skin. To compare the effects of different energy level, we used two different parameters, 84W and 200W for 2 seconds. The wound healing response was evaluated histologically and by RT-PCR up to 10 weeks post-RF treatment. Changes of diameter of collagen fiber were assessed by electron microscopy. Results: Dermal thickness, cellularity, and elastin content increased. RT-PCR studies revealed an immediate increase in IL-1b, TNF-a, and MMP-13 while MMP-1, HSP70.2, HSP47, and TGF-b levels increased by 2 days. We also observed a marked induction of tropoelastin, fibrillin, as well as collagen type 1 and 3 by 28 days post-treatment. Conclusion: Our study revealed a vigorous wound healing response is initiated post-treatment with progressive increase in inflammatory cell infiltration from day 2 through 10 weeks. Furthermore, we successfully demonstrated for the evidence of profound neocollagenesis and neoelastogenesis following RF treatment of human skin. The combination of neoelastogenesis and neocollagenesis induced by treatment with the GFR system may provide a reliable treatment option for skin laxity and rhytids.

Elevated HDL2-paraoxonase and reduced CETP activity are associated with a dramatically lower ratio of LDL-cholesterol/total cholesterol in a hypercholesterolemic and hypertriglyceridemic patient.

Lee, Ji-Hye,Park, Jung-Heun,Lee, Sang-Hak,Kim, Jae-Ryong,Cho, Kyung-Hyun D.A. Spandidos 2010 INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE Vol.25 No.6

<P>A female patient (64 years of age; body mass index, 26) had a markedly and relatively low low-density lipoprotein-cholesterol (LDL-C) level (97 mg/dl) despite high serum total cholesterol (TC) (331 mg/dl) and triacylglyceride levels (307 mg/dl). Since the expected LDL-C was 222 mg/dl, there was a significant difference between the calculation and measurement based on direct enzyme assay. Only 30% of serum cholesterol was associated with LDL-C in this patient. To determine the basis for the markedly low LDL-C/TC ratio, we isolated and analyzed lipoproteins from the patient as well as age- and gender-matched controls. The patient had lowered serum CETP activity and elevated paraoxonase activity with GOT and GPT values in the normal range. The very low-density lipoprotein particles from the patient were larger than those of the controls and enriched with lipid and protein, while the LDL from the patient (LDL-P) had a lower particle number and protein content than the controls. The LDL-P was more resistant to cupric ion-mediated oxidation. HDL2 from the patient (HDL2-P) had highly enhanced paraoxonase activity and antioxidant ability. The patient had a 1.5-fold higher level of apolipoprotein (apo) A-I expression in HDL2. ApoA-I in HDL2 and HDL3 from the patient showed no fragmentation, while the control had fragmented bands (17 and 21 kDa) in the HDL. The HDL2-P also had a larger particle size and greater protein content with less lipid content. HDL3-associated cholesteryl ester transfer protein was reduced in the patient, although the particle size was similar to the controls. In conclusion, a patient who had a markedly lower LDL-C/TC ratio despite hyperlipidemia associated with higher paraoxonase activity, higher apoA-I level and lower CETP activity without fragmentation of apoA-I in the HDL fraction is presented. The enhanced antioxidant and anti-inflammatory activity of HDL might contribute to the low LDL-C/TC ratio in this patient.</P>

Aster ageratoides Turcz. extract attenuates Alzheimer’s disease-associated cognitive deficits and vascular dementia-associated neuronal death

Ji Heun Jeong,Seung Eun Lee,Jeong Hoon Lee,Hyung Don Kim,Kyung-Hae Seo,Dong Hwi Kim,Seung Yun Han 대한해부학회 2020 Anatomy & Cell Biology Vol.53 No.2

Dementia is the common neurodegenerative disorder affecting the elderly, with a progressive cognitive decline and memory loss. Since Alzheimer’s disease (AD) and vascular dementia (VD) share key pathologies including oxidative damage, oral supplement of phytochemical medicines, which are well-known for their antioxidant properties, can be a viable therapy for both types of dementia. In this study, the therapeutic potential of the Aster ageratoides extract (AAE), an oriental drug with multiple medicinal properties, was tested on experimental rat models of AD and VD. After confirming the in vitro attenuation of neuronal excitotoxicity by AAE, rats were orally administered with AAE for 7 days and subsequently tested under 2 different experimental paradigms: efficacy screening against #1 AD and #2 VD. For paradigm #1, the rats received intraperitoneal scopolamine and subsequently underwent 3 different behavior tests i.e., the Y-maze, novel object recognition, and passive avoidance tests. For paradigm #2, the rats were operated with the 2-vessel occlusion and hypovolemia (2VO/H) technique, and at postoperative day 7, their hippocampal neuronal viability and the neuroinflammatory changes were quantified. The results showed that the scopolamine-induced impairment of memory performance was significantly improved by AAE intake. Furthermore, while the 2VO/H operation induced marked hippocampal neuronal death and microglial activation, both these effects were significantly attenuated by AAE supplements. Some of the aforementioned effects of AAE intake were dose-dependent. These results provided evidence that AAE supplements can exert anti-AD and -VD efficacies and suggested that AAE might be used as an edible phytotherapeutic for the 2 major types of dementia.