Effects of Phenytoin and Diazepam on the Seizure Activity in the Cortical Dysplasia Animal Models

Kim, Si-Hyung,Choi, In-Sun,Cho, Jin-Hwa,Park, Eun-Ju,Jang, Il-Sung,Choi, Byung-Ju,Kim, Hyun-Jung,Kim, Young-Jin,Nam, Soon-Hyeun The Korean Academy of Oral Biology 2006 International Journal of Oral Biology Vol.31 No.2

Dysplasia-associated seizure disorders are markedly resistant to pharmacological intervention. Relatively little research has been conducted studying the effects of antiepileptic drugs(AEDs) on seizure activity in a rat model of dysplasia. We have used rats exposed to methylazoxymethanol acetate(MAM) in utero, an animal model featuring nodular heterotopia, to investigate the effects of AEDs in the dysplastic brain. Pilocarpine was used to induce acute seizure in MAM-exposed and age-matched vehicle-injucted control animals. Field potential recordings were used to monitor amplitude and numbers population spikes, and paired pulse inhibition in response to stimulation of commissural pathway. Two commonly used AEDs were tested: diazepam 5, 2.5mg/kg;phenytoin 40, 60mg/kg. Diazepam(DZP) and phenytoin(PHT) reduced the amplitude of population spike in control and MAM-exposed rats. However, the amplitude of population spike was nearly eliminated in control rats as compared to the MAM-exposed rats. Pharmaco-resistance was tested by measuring seizure latencies in awake rats after pilocarpine administration(320mg/kg, i.p.) with and without pretreatment with AEDs. Pre-treatment with PHT 60 mg prolonged seizure latency in control rats, but not in MAM-exposed animals. The main findings of this study are that acute seizures initiated in MAM-exposed rats are relatively resistant to standard AEDs assessed in vivo. These data suggest that animal model with cortical dysplasia can be used to screen the effects of potential AEDs.

Lidocaine, Thrombin, Epinephrine 의 항균효과

김진우,이동건,전혜선,김승준,김석찬,안중현,김치홍,권순석,김영균,김관형,문화식,신완식,송정섭,박성학 대한감염학회 2005 감염과 화학요법 Vol.37 No.6

목적 : 기관지내시경 검사에서 흔히 사용되는 국소마취제인 lidocaine과 내시경시 지혈목적으로 사용되는 thrombin과 epinephrine이 각종 균주에 미치는 항균효과에 대해 알아보고자 하였다. 재료 및 방법 : 균주는 가톨릭대학교 성모병원에서 2004년 3월부터 2004년 9월까지 임상검체 에서 동정된 S. aureus, S. pneumoniae, K. pneumoniae, P. aeruginosa 각각 42, 42, 42, 43주를 대상으로 하였다. Lidocaine, thrombin, epinephrine 감수성 검사는 National Committee for Clinical Laboratory Standards (NCCLS, 2002)의 기준에 따랐다. 결과 : Lidocaine은 S. aureus, S. pneumoniae, P. aeruginosa에서 MIC_(50), MIC_(90) 모두 20,000 ㎍/mL 이었다. K. pneumoniae는 각각 10,000 ㎍/mL이었다. Thrombin은 S. aureus와 P. aeruginosa에서 MIC50 500 lU/mL 과 MIC_(90) 500 IU/mL 이상이었고, K. pneumoniae에서는 MIC_(50)과 MIC_(90)이 모두 500 lU/mL이상이었으나 S. pneumoniae에서는 MIC_(50)과 MIC_(90)은 125 IU/mL이었다. Epinephrine은 K. pneumoniae, S. pneumoniae에 대한 MIC_(50), MIC_(90)가 모두 >500 ㎍/mL이었고, S. aureus와 P. aeruginosa에 대한 MIC_(50), MIC_(90)가 모두 500 ㎍/mL이었다. 결론 : 기관지 내시경 검사에서 흔히 쓰이는 lidocaine, thrombin, epinephrine 등의 약제들이 호흡기 질환의 흔한 병원균인 S. aureus, S. pneumoniae, K. pneumoniae, P. aeruginosa 균주들에 대해 항균 효과가 있을 수 있어 세균배양검사에 영향을 미칠 수 있겠다. Background : We performed this stody to find out about antimicrobial effect of lidocaine which is commonly used local anesthetic, and thrombin and epinephrine used for hemostasis during bronchoscopic procedures. Materials and Methods : The microorganisms that were cultured from specimens obtained during bronchoscopy were Staphylococcus aureus (n=42), Streptococcus pneumoniae (n=42), Klebsiella pneumoniae (n=42), and Pseudomonas aeruginosa (n=43) collected from St. Mary's Hospital, from March to Sep 2004 were used for susceptibity testing. Susceptibility to lidocaine, thrombin, and epinephrine were tested according to the National Committee for Clinical Laboratory Standards. Result : MIC_(50) and MIC_(90) of lidocaine for S. aureus, S. pneumoniae, P. aeruginosa were all 20,000 ㎍/mL and that for K. pneumoniae were 10,000 ㎍/mL. MIC_(50) and MIC_(90) of thrombin for both S. aureus and P. aeruginosa was 500 IU/mL and above 500 IU/mL, respectively; that for K. pneumoniae were all above 500 IU/mL and for S. pneumoniae they were 125 IU/mL, MIC_(50) and MIC_(90) of epinephrine for K. pneumoniae and S. pneumoniae were above 500 ㎍/mL; that for S. aureus and P. aeruginosa were 500 ㎍/mL. Conclusion : We observed possible antimicrobial effect of lidocaine, thrombin, and epinephrine in vitro against pathogens such as S. aureus, S. pneumoniae, K. pneumoniae, P. aeruginosa, which are common respiratory microorganisms. The use of these agants could affect the result of bacterial culture.

빈랑자 추출물의 염색성 및 기능성에 관한 연구

김지선,조용석,최순화 대한가정학회 2003 Family and Environment Research Vol.41 No.7

Recently there has been interested in the use of natural dyes in textile applications. Generally natural dyes can exhibit better biodegradability and have a higher compatibility with the environment. In this study, cotton wool and silk fabrics were dyed with alkali extract of Arecae semen using various mordants, and their dyeabilities were discussed. Additionally the fastness to washing, perspiration, light, rubbing, and drycleaning and UV-B protection rate were also investigated. Cotton, silk and wool fabrics dyed with alkali extract of Arecae semen were colored in reddish yellow tint. The optimum dyeing condition of the colorants extracted from the Arecae semen was three repeated dyeing at 95℃ for 1.5hrs. The dyed fabrics by using mordanting methods improved the fastness to washing, perspiration and drycleaning, but the fastness to light and rubbing were not increased. The UV-B protection rate of the wool fabrics were increased by dyeing with extract of Arecae semen.

ATM 망에서의 VDT 제공 방안

김영선,김화종,정명순 강원대학교 정보통신연구소 1997 정보통신논문지 Vol.1 No.-

VDT was proposed in order to provide residential multimedia services such as digital video, voice, data and so on, We studied a VDT network architecture and MPEG-2 transmission scheme over an ATM network. For this purpose, we studied the network architecture and communication protocols for MPEG-2 over AAL 5. In a hierarchical video coding, a picture can be seperated into the basic and the higher layers. The basic layer coding is the minimal information needed to reconstruct the video, and the higher layer coding is used to make better quality. By simulation, we obtained the cell loss probability caused by the overflow of video traffic, when subscribers received MPEG-2 video through an ATM network.

울금(鬱金)색소에 의한 염색견뢰도에 관한 연구

김정숙,최순화 대구산업정보대학 1994 논문집 Vol.8 No.-

A study on the colorfastness of turmeric cotton and silk fabrics were dyed by dyestuff extracted from turmeric and treated with four kinds of mordant. Then colors of the treated fabrics were specified by the method of XYZ color system and the colorfastness was tested for laundry, light. The result of experiment were analyzed by color difference according to L^(*)a^(*)b^(*) value formula. From the present study, following results were obtaianed. 1. Dyeing properties of tumeric silk beacme greater than on cotton. 2. Cu mordant did to laundy best of all. 3. Colorfastness to light was not good, cu mordant did to light best of all.

노인 구강건강관리 실태에 대한 연구

김수화,권순복 경복대학 2002 京福論叢 Vol.6 No.-

본 연구는 고령화사회에 접어든 우리나라의 노인구강건강을 증진시키기 위한 기초자료를 마련하고자 서울특별시에 거주하는 60세 이상 노인을 대상으로 일반사항과 구강상태, 구강보건인식도, 치과진료 및 구강보건교육 경험도에 대한 설문을 실시하여 다음과 같은 결론을 얻었다. 1. 조사대상자는 모두 177명으로 여자 81.4%, 남자 18.6%였고, 연령은 70-74세 27.7%, 65-69세·75-79세가 각각 19.8% 였으며, 학력은 무학이 49.2%로 가장 많았고 초등학교 졸업이 36.2%로 나타났다. 거주형태는 자녀와 함께 사는 경우가 58.2%, 혼자(부부) 사는 경우 41.8%였으며, 직업은 무직인 경우가 90.4%로 많았고, 전신질환은 58.2%가 가지고 있지 않았다. 2. 조사대상자의 구강 내 증상은 저작장애가 64.4%로 가장 많았고, 잔존치아 개수는 25개 이상이 26.0%, 계속가공의치는 대상자의 56.5%가 장착하고 있었고, 대상자의 56.4%가 구강건강이 좋지 않다고 인지하고 있었다. 의치필요자는 총 66.7%, 의치장착자는 총 44,1%로 필요한데 장착하지 못한 노인이 33.9%였다. 잇솔질 횟수는 하루 2회가 50.8%, 시기는 아침식사 후 85.9%, 저녁식사 후 76.3%, 방법은 좌우로 38.4%, 위아래 20.9%, 세치제는 치약이 80.8%로 많았다. 치실과 스케일링 인지도는 각각 16.9%, 36.2%로 나타났고, 음식물제거는 이쑤시개 사용이 46.3%, 치실 3.4%, 구강병대처법은 치과내원이 62.7%로 조사되었다. 최근 1년동안 치과방문경험도는 45.2%, 무면허진료와 구강보건교육 경험도는 46.9%, 18.1%였으며, 우선적으로 필요한 치료는 의치와 보철이 각각 26.6%, 22.6%로 많았다. 3. 연령이 높을수록 의치장착도와 필요도는 높게, 계속가공의치 장착도는 낮게 나타났으며, 저작장애는 연령이 증가함에 따라 크게, 잔존치아 개수는 연령이 증가하면서 감소하는 것으로 나타났다. 치실과 스케일링에 대한 인지도는 연령이 증가함에 따라 낮았고 혼자(부부)사는 노인이 무면허진료 경험도가 56.8%로 높았다. 구강건강이 약하다고 인식할수록 무면허진료 경험도가 높게 나타나 유의한 차이를 보였다. This Thesis was made to offer the data for oral health of the old and to improve their oral health. The subjects of this study were male and female old people who were older than 60 and have lived in Seoul. The questionnaire was made up of population sociological characteristics, oral condition, recognition of oral hygiene and experience of treatment by unlicensed and oral health instruction. The results were as follows : 1. The subjects consisted of 81.4% female and 18.6% male, 27.7% were 70 to 74 in age, 49.2% of the surveyees had never gone to school. In living type 58.2% of the subjects have lived with their children, the unemployed are 90.4% and 58.2% of the subjects had no general disease. 2. 64.4% of the surveyees had masticatory dysfunction, the rate of remained teeth is 26.0% in over 25. 56.4% responded their oral health is weak. The proportion of people wearing denture was 44.1% of the people who need denture and 33.9% in need of denture had no denture. In survey on toothbrushing, 50.8% brushed their teeth twice a day, 85.9% brushed after breakfast and 38.4% used their toothbrush up and down. The recognition of a dental floss and scaling is 16.9% and 36.2% respectively. 46.3% used toothpick in the way to clean food debris and 45.2% visited dental clinic within a year. The rate of experience of the treatment by unlicensed and oral health instruction is 46.9% and 18.1% respectively. 3. The rate of wearing denture, need for denture and masticatory dysfunction is higher and recognition of floss and scaling is lower at the older subjects. The old living alone(coup1e) had higher rate of experience of treatment by unlicensed than the old living with their children.

배양상태의 흰쥐 뇌하수체 앞엽에서 Neuropeptide Y 함유세포에 관한 면역조직화학적연구

이화영,김순회,이희래,어환,이왕재,백상호,이병란 대한해부학회 1996 Anatomy & Cell Biology Vol.29 No.3

양호교사 자격연수교과과정 개발에 관한 연구

김화중,김영임,윤순녕 韓國學校保健學會 1996 韓國學校保健學會誌 Vol.9 No.1

School health promotion is related to reinforce on various roles of school health teachers including health instruction, health services, healthful school living. In this aspects, there would be necessory for qualified continuous education proceed to activate roles of school health teachers. So the objectives of this study are first, to develop curriculum framework for inservice training of health school nurses, second, to descrive of curriculum objectives, third, to state of curriculum objectives and learning methods. There were used study methods as literature review, expert conferencing and workshop. Major results are as follows; 1. Curriculum framwork for inservice training of health school nurses was constitute of major variables of health care model and direct services role of school health teacher such as health service, health instruction and healthful environment area. 2. The curriculum for health instruction were different from elementary school and middle school. 3. Time distribution for curriculum was composed of that health service is 12hours, health instruction is 96 hours and healthful environmenet is 12 hours. 4. The learning methods for inservice training were suggested to intensify positive attitude through the discussion and practical exercise. 5. The curriculum objectives were classified by total, area and a course of study and time summary table was suggested. In conclusion, the developed curriculum would be contribute to improve of practical ability for school health teachers. And to increase of inservice training effect, there should be operate in order to participate trainees activately and to be educate concurrently. Also, there are necessary for execute in series of the program such as standized text developing by course, evaluation index developing and instructor education etc.

방사선사진에서의 골질과 임상적으로 평가한 골질 분류의 상관관계

김현우,허경회,박관수,김정화,이원진,허민석,이삼선,최순철 대한구강악안면방사선학회 2006 Imaging Science in Dentistry Vol.37 No.1

Purpose : To investigate the validity of digital image processing on panoramic radiographs in estimating bone quality before endosseous dental implant installation by correlating bone quality in radiographic images with clinical bone quality classification. Materials and Methods : An experienced surgeon assessed and classified bone quality for implant sites with tactile sensation at the time of implant placement. Including fractal dimension eighteen morphologic features of trabecular pattern were examined in each anatomical sites on panoramic radiographs. Finally bone quality of 67 implant sites were evaluated in 42 patients. Results : Pearson correlation analysis showed that three morphologic parameters had weak linear negative correlation with clinical bone quality classification showing correlation coefficients of -0.276, -0.280, and -0.289, respectively (p<0.05). And other three morphologic parameters had obvious linear negative correlation with clinical bone quality classification showing correlation coefficients of -0.346, -0.488, and -0.343 respectively (p<0.05). Fractal dimension also had a linear correlation with clinical bone quality classification with correlation coefficients -0.506 significantly (p<0.05). Conclusion : This study suggests that fractal and morphometric analysis using digital panoramic radiographs can be used to evaluate bone quality for implant recipient sites. (Korean J Oral Maxillofac Radiol 2006; 36 : 25-32)

대동맥 협착증과 동반된 출혈성 위장관 동정맥 기형에서 Estrogen-Progesterone 치료 1예

김도연,김화정,김효정,남준식,박수영,박영미,신정은,주미순,황민규,정혜경,손혜영,이선영,이한주 大韓消化器內視鏡學會 1999 Clinical Endoscopy Vol.19 No.부록2