Compensated Gross Registered Tonnage의 槪要

이희일(李熙壹),박용철(朴容喆) 대한조선학회 1981 大韓造船學會誌 Vol.18 No.2

병적 담낭 상피세포내 점액질의 조직화학적 관찰

이희래,김순희,조정실,김옥경 梨花女子大學校 醫科大學 醫科學硏究所 1978 EMJ (Ewha medical journal) Vol.1 No.1

The present study was performed to evaluate the histochemical properties of epithelial mucins in diseased gallbladders. 18 gallbladder specimens were selected in which metaplastic goblet cells had been observed. Of the specimens 9 were gallstone and 9 cholecystitis. For microscopic examination, tissues were sectioned at a thickness 5μm, and were stained with following histochemical techniques: Alcian blue at pH 1.0 or 2.5^13) and periodic acid Schiff's reaction (PAS).^12) Observed results were as follows: 1) In ordinary gallbladder the epithelial cells showed a predominance of sulphated mucosubstance. 2) In many case of cholelithiasis a typical goblet-typed epithelial cells were found in the top of folds. In large goblet cell area the relative proportion of sulphated acid mucin decreased and correspondingly non-sulphated acid mucin increased. 3) In the case of cholecystitis the intracellular mucin of high columnar epithelium predominated in the deep area of folds. 4) The neutral mucin predominated in the surface epithelium of gallbladder under pathological condition.

흰쥐 뇌 선조체내 신경세포의 전자현미경적 관찰

이희래 梨花女子大學校 醫科大學 醫科學硏究所 1981 EMJ (Ewha medical journal) Vol.4 No.3

The present study was done to define the morphological characteristics of nenurons in the rat neostriatum. The rats were perfused with 1% paraformaldehyde and 2% glutaraldehyde fixative in phosphate buffer at pH 7.4. Tissues from caudate nucleus were postfixed with 2% osmium tetroxide in phosphate buffer at pH 7.4. and dehydrated in graded ethanol and aceton, and embedded in Epon 812. The polymerization was carried out in a 60℃ oven. The ultrathin sections were contrasted with uranyl acetate and lead citrate, and examined with a Siemens Elmiskop IA. Four morphologically differenttypes of neurons were classified. (1) The large neurons were often elongated or spindle shapes, measured 25~30μm in diameter. They had an indented nucleus and contained large amount of cytoplasm with many stacks of granular endoplasmic reticulum, concentration of ribosomes and rich Golgi cisternae. (2) The medium-sized, polygonal neurons were pyramid or polygonal shapes, measured 14~20μm in diameter. All neurons of this type had a spherical, unindented nucleus and moderate cytoplasm of pale cytoplasm with relatively small amount of granular endoplasmic reticulum and riboromes. (3) The medium sized, ovoid neurons were the most common neostriatal cells. These neurons were ovoid or spherical shapes, measured 12~20μm in diameter. They contained small quantities of Golgi cister in the dark cytoplasm. (4) The small neurons were the least common cells in the striatum. They had a distinctive nucleus, usually with a single indentation and contained little Glogi apparatus and few cisternae of granular endoplasmic reticulum.

원숭이 腦 靑斑의 電子顯微鏡的 觀察

李熙來 梨花女子大學校 韓國生活科學硏究院 1982 韓國生活科學硏究院 論叢 Vol.29 No.-

본 실험은 청반내에 분포하는 신경세포의 미세구조를 관찰하기 위하여 시도되었다. 실험동물인 다람쥐원숭이를 nembutal 마취하에 고정액인 1% paraformaldehyde와 2% glutaraldehyde(phosphate buffer pH 7.4)로 전고정하고, 2% osmiumtetroxide(phosphate buffer pH 7.4)로 후고정하여 Epon 812에 포매한 후 전자현미경 표본을 작성하여 관찰한 바 청반의 신경세포는 중형세포와 소형세포로 구분되고, 중형세포는 다시 축삭세포와 체연접의 수에 따라서 연접이 많은 세포와 적은 세포로 구분되며, 일부 축삭세포체연접은 세포체극에서 형성된다. The neuronal cell bodies of the locus coeruleus ofthe monkey (Saimiri sciureus) were investigated using electron microscopy. On the basis of size and shape of cell body, branching pattern of dendrites, distribution of cytoplasmic organelles and number of axosomatic synapses the locus coeruleus was observed three types of neuronal perikarya; medium-sized polygonal, medium-sized ovoid and small neurons. The medium-sized polygonal neurons were polygonal shapes measured to be 10~26㎛ in diameter. These neurons had thick dendrites with numerous branchings and small number of axosomatic synapses. The medium-sized ovoid neurons were ovoid or spherical shapes measured to be 13~23㎛ in diameter. All neurons of this type were characterized by a high number of axosomatic synapses. The small neurons(9~21㎛) displayed pale cytoplasm containing few organelles, Most of the axosomatic synapses make a direct contact to the soma but some of them do by somatic appendages.

우리가 떠나온 집, 시골집

이희인(李羲仁) 새얼문화재단 2011 황해문화 Vol.72 No.-

흰쥐 선조체 및 흑질의 Serotonin 수용체에 대한 ^3H-ketanserin을 이용한 전자현미경 자기방사법적 연구

이희래,이혜성,배영숙 이화여자대학교 생명과학연구소 1990 생명과학연구논문집 Vol.1 No.-

흰 쥐의 측뇌실에 ^3H-ketanserin을 주입한 다음 신줄무늬체 및 흑색질에서 serotonin 수용체의 분포양상을 전자현미경 자기방사법으로 관찰하여 다음과 같은 결과를 얻었다. 신줄무늬체에서 은입자는 대부분이 가지돌기 및 축삭가지와 연접에 표지되었다. 표지된 연접에서 축삭종말은 소원형 및 다형소포를 함유하였고 비대칭의 축삭가지돌기연접을 형성하였다. 흑색질에서 표지양상은 신줄무늬체와 거의 같았다. 이러한 결과에 따라서 신줄무늬체 및 흑색질에서 serotonin-2 수용체는 주로 소원형 및 다형소포를 함유한 비대칭의 축삭가지돌기연접에 분포하며 이 수용체의 분포는 serotonin신경섬유의 분포와 밀접한 관련이 있는 것으로 생각된다. It has been demonstrated that some serotoninergic axonal branches from the dorsal rap heneurons prject to the neostriatum and the substantia nigra, and also has been reported that serotonin receptors distribute in the same regions of the mammalian brain. It is interesting to analyse how the distribution of serotonin receptors correlates with that of serotoninergic nerve terminals in the brain. This study was performed to identify the serotoninergic nerve terminals in the neostriatum and the substantis nigra of the rat. Identification of the serotoninergic nerve terminals at ultrastructural level has been done by autoradiography after cerebroventricular administration of ³H-ketanserin as a ligand. In the neruropil of the neostriatum, most of silver grains were labeled on the thin branches of axons and dendrites and some of them on soma or myelinated axons. In the cases of labeled synapes, axon terminals contained round and pleomorphic vesicles and showed asymmetric contact with dendrites or dendritic spines. In the substantia nigra, the distribution patterns of labeled siver grains beared a close paralell to neostriatum. But in the labeled synapes from this region, axon terminals ocntaining small round vesicles made mainly asymmetric synapses with dendrites. The observations made in this study indicate that serotonin-2 receptors are present in the asymmetric axo-dendritic synapses with small round and pleomorphic vesicles and the distribution of the serotonin-2 receptors have closely with that of serotoninergic fibers in the neostriatum and the substantia nigra of rat.

흰쥐 편도체의 Dopamine 수용체에 대한 전자현미경 자기방사법적 연구

이희래 梨花女子大學校 醫科大學 醫科學硏究所 1989 EMJ (Ewha medical journal) Vol.12 No.1

The present study was performed to identify the dopaminergic receptor sites in rat amygdala. Identification of the receptor sites at ultrastructrual level has been done by autoradiography after cerebral ventricular perfusion of ^3H-haloperidol. From 434 examples of silver grains, 5% were labeled in soma, 36% in thick dendrites, 26% in the thin branches of axons and dendrites, 21% in thick axons and terminals and 12% in synapses with synaptic vesicles. In the cases of labeled synapses, all of axon terminals contained small round vesicles and showed asymmetric contact with dendrites. By these results it may be implied that dopamine receptors in the amygdala are located in both pre-and post-synaptic sites of the asymmetric axo-dendritic synapses with small round vesicles and the axons of labeled synapses originated from the dopaminergic neurons in the midbrain.

Harmaline이 마우스 Paneth세포에 미치는 영향에 대한 광학 및 전자현미경적 연구

이희래,최월봉,권흥식 대한해부학회 1976 Anatomy & Cell Biology Vol.9 No.2

원숭이 뇌 청반 신경연접의 전자현미경적 관찰

이희래,김순회 梨花女子大學校 醫科大學 醫科學硏究所 1982 EMJ (Ewha medical journal) Vol.5 No.3

This study provides a description of the normal morphology of the synapses of the locus coeruleus in the squirrel monkey, Saimiri sciureus, at the electron microscopic level. The animals were perfused with 2% glutaraldehyde-1% paraformaldehyde fixative in phosphate buffer at pH 7.4. Tissues from the locus coeruleus were postfixed by 2% osmium tetroxide in phosphate buffer at pH 7.4, and dehydrated in graded ethanol and aceton, and embedded in Epon 812. The polymerization was carried out in oven at 60℃. The ultrathin sections were cut on a LKB ultratome, contrasted with uranyl acetate and lead citrate, and examined in a Siemens Elmiskop 1A electron microscope. Special attention during the observation was given to the different types of synapses. Five types of synapses with many characteristics, could be differentiated : small-round vesicle terminals(52%), pleomorphic vesicle terminals(25%), elongated vesicle terminals(16%), small-round and dense core vesicle terminals(5%) and large-round vesicle terminals(2%). Some of the axosomatic synapses with small round or pleomorphic vesicles make an indirect contact to the soma by somatic appendages.

흰쥐 뇌의 Serotonin-2 수용체 분포에 대한 자기방사법적 연구

이희래 梨花女子大學校 醫科大學 醫科學硏究所 1988 EMJ (Ewha medical journal) Vol.11 No.4

The distribution of serotonin-2 receptors in the rat brain was studied by light microscopic autoradiography, using〔^3H〕ketanserin as a ligand. The serotonin-2 receptor densities were quantified by an actual count of labeled silver grains from the tissue preparations. A heterogeneous distribution of serotonin-2 receptor densities was found in the rat brain. High concentrations of serotonin-2 receptors were localized over the superior colliculus, neostriatum, amygdaloid complex and mammillary body. Intermediate concentrations were found over the dentate gyrus and visual cortex(area 17). Low densities were found over the substantia nigra and olfactory tubercie. The observations made in this study indicate that the anatomical distribution of serotonin-2 receptor is in accord with the distribution of serotoninergic innervation and the densities of serotonin-2 preceptor are differences in brain area of rat.