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      • KCI등재

        B16 Melanoma 세포에서 Phenylprlopanoid 화합물이 Melanin 생성에 미치는 영향

        박영미,윤미연,김경원,조남영,임혜원,이지윤,이진희,김연정,김창종 대한약학회 2003 약학회지 Vol.47 No.6

        '스콜라' 이용 시 소속기관이 구독 중이 아닌 경우, 오후 4시부터 익일 오전 7시까지 원문보기가 가능합니다.

        To investigate the relationship between structure and biological activity of phenylpropanoids, we measured effects of phenylpropanoids on anti-oxidant and whitening activity, In DPPH radical scavenging activity, caffeic acid analogues showed the significant anti-oxidant activity. Although phenylpropanoids did not inhibit purified-tyrosinase activity, they significantly inhibited tyrosinase activity and melanin production in MSH-stimulated B16 melanoma cells. However, phenylpropanoids did not affect tyrosinase expression in MSH-stimulated B16 melanoma cells, which suggest that inhibition of MSH-induced melanin production was due to tyrosinase inhibition mediated via other signal pathways but not expression of tyrosinase. Phenylpropanoids also significantly inhibited both hyaluronidase and elastase activity, suggesting that phenylpropanoids may be used as whitening, hydration and anti-wrinkling agents. Hydroxyl residue of aromatic ring in phenylpropanoids plays an important role in anti-oxidant and whitening activity.

      • KCI등재후보

        韓·日 문사의 교유시를 통해 본 상호인식-秋潭 兪瑒과 하야시 라잔(林羅山)을 중심으로-

        박영미 근역한문학회 2010 漢文學論集 Vol.30 No.-

        This writing is on Yuchang(1614~1692)and Hayashi Razan’ poems exchange. Yuchang visited Japan as a member of Korean Envoys and wrote poem with Hayashi Razan in 1655. It analyzed Yuchang and Hayashi Razan’s poems at the position of the subject and the other. They were subjects and at the same time others to each other. They who met each other clearing a geographical obstacle like a sea had no excitement and fear. Instead, ‘conflicts between subjects’ held a place at the position. Then, where are the conflicts from? This was made by East Asian situation at that time. That was diplomacy phenomena that deeply reflect establishment and fluctuations of Japan’s Baku government while reflect rehabilitation and development fluctuations of official government of Chosun Dynasty. 이 글은 1655년, 兪瑒(1614~1692)은 조선통신사 일행으로 일본을 방문하여 林羅山과 수창한 시를 분석한 것이다. 兪瑒의 「扶桑途中述懷兼敍壯遊一百五十韻錄示九巖茂源兩老師求和」와 林羅山의 「和秋潭扶桑壯遊一百五十韻」를 주체와 타자의 입장에서 분석하였다. 이들은 주체이며 동시에 서로에게 타자였다. 바다라는 지리적 장애물을 넘어 만난 이들이지만 서로에게 느끼는, 낯섦에 대한 흥분과 두려움은 없었다. 대신 그 자리는 ‘주체간의 충돌’이 자리하고 있었다. 소중화주의와 일본형 화이론의 대립으로 설명하기엔 이것은 좀 더 복합적이고 내면화되어 있었다. 그렇다면 이런 주체간의 충돌은 어디서 기인하는가? 이것은 당시 동아시아의 상황이 만들어낸 것이었다. 일본의 경우는 막번 체제의 성립과 동요, 조선의 경우는 관인지배의 재흥과 발전 동요를 깊이 반영한 외교현상이었기 때문에 충돌이 발생하였다.

      • KCI등재

        Fungi-rice bran based Fermentation of Coptis Chinensis and Curcuma Longa Root and its Influence of Silk Dyeing

        박영미,최재홍 한국의류산업학회 2013 한국의류산업학회지 Vol.15 No.4

        This study examined the dye-properties of natural fabrics dyed with Coptis chinensis and Curcuma longa root fermented with fungi. The optimum culture conditions for the fermentation of microorganisms, the relationship between natural dye color and fermentation conditions were investigated. Two different medical herbs (ground to 80-100 mesh in size) were used as a natural dyeing source. Phellinus linteus (P. linteus), which can grow in different media, such as Agarmedium (only agar containing medium), maltose extract agar (MA) and potato dextrose extract agar (PDA) culture media,were isolated from the medium. P. linteus was confirmed to be the optimum microorganism for the fermentation of Coptis chinensis and Curcuma longa, and the MA medium was confirmed to be the best for culturing. When using the microorganism as the fermenting agent, 32oC was found to be the optimum fermenting temperature for both natural colorants. Regarding the dyeing property of the fermented natural dye, silk was dyed quite darkly in an appearance by naked eye estimation and the K/S value in the color strength of silk reached a high level of 16 after the fermenting process. The washing fastness of dyed silk after treatment washing was reduced from 4 to under4 and indicates that dyed silk with fermented plant was not unsubstantial. The light fastness was 1 to 2, showing intended to maintain due to the fermentation process.

      • KCI등재

        팥(Phaseolus angularis) 열수 추출물의 산화적 DNA와 세포 손상 억제 효과

        박영미,정진부,서주희,임재환,정형진,서을원 한국자원식물학회 2011 한국자원식물학회지 Vol.24 No.2

        '스콜라' 이용 시 소속기관이 구독 중이 아닌 경우, 오후 4시부터 익일 오전 7시까지 원문보기가 가능합니다.

        본 연구에서는 열수 팥 추출물이 hydroxyl 라디칼에 의해 유도되는 산화적 스트레스에 미치는 영향을 알아보기 위하여 항산화활성과 DNA 및 세포의 산화적 손상 억제 효과를 조사하였다. 팥 열수 추출물의 DPPH 라디칼과 hydroxyl 라디칼의 제거능은 다소 낮았으나, Fe2+-chelating과 과산화수소 제거효과는 높게 나타나 활성산소의 생성을 억제하는 데 효과적인 것으로 확인되었다. 또한 팥 열수 추출물의 in vitro DNA cleavage, DNA migration 및 H2AX의 인산화비 억제활성은 높은 활성을 보여주고 있어 라디칼에 의한 DNA 손상 억제에 효과적으로 작용하였다. 또한 지질과산화와 p21의 발현율을 통해 세포의 산화적 손상에 미치는 영향을 살펴보면 지질과산화 억제능과 p21의 발현율에 매우 효과적으로 작용하고 있어 라디칼에 의한 산화적 스트레스로부터 세포를 보호할 것으로 생각된다. In this study, we evaluated the protective effects of the hot water extract from red bean (Phaseolus angularis) against oxidative DNA and cell damage induced by hydroxyl radical. The antioxidant activities were evaluated by hydroxyl radical and hydrogen peroxide scavenging assay, and Fe2+-chelating assay. Although the extract with hot water didn’t scavenge the hydroxyl radical, it removed and chelated hydrogen peroxide and ferrous iron necessary for the induction of hydroxyl radical by 71% and 64% at 200 μg/ml, respectively. Its protective effect on oxidative DNA damage was carried using ψX-174 RF I plasmid DNA comparing the conversion level of supercoiled form of the plasmid DNA into open-circular form and linear form and the expression level of phospho-H2AX in NIH 3T3 cells. In ψX-174 RF I plasmid DNA cleavage assay, it inhibited oxidative DNA damage by 96% at 200 μg/ml. Also, it decreased the expression of phospho-H2AX by 50.1% at 200 μg/ml. Its protective effect against oxidative cell damage was measured by MTT assay and the expression level of p21 protein in NIH 3T3 cells. In MTT assay for the protective effect against the oxidative cell damage, it inhibited the oxidative cell death and the abnormal cell growth induced by hydroxyl radical. Also, it inhibited p21 protein expression by 98% at 200 μg/ml. In conclusion, the results of the present studies indicate that hot water extract from red bean exhibits antioxidant properties and inhibit oxidative DNA damage and the cell death caused by hydroxyl radical.

      • KCI등재

        A Study on Sensory Properties of Backsulgi using Dry Non-Glutinous Rice Flour

        박영미,윤혜현 (사)한국조리학회 2014 한국조리학회지 Vol.20 No.5

        The study explores the sensory properties of Backsulgi prepared with dry non-glutinous rice flour sweetened with various sweeteners(sugar, honey, oligosaccharide, trehalos, erythritol and accesulfame K). Sensory attributes of Backsulgi were evaluated by quantitative descriptive analysis(QDA), PCA and PLSR. The QDA results revealed that the sample sweetened with trehalose showed highest value in dryness, and samples with accesulfame K, honey and erythriol had relatively high levels in moisture and springiness. Principle component analysis (PCA) results showed 78.89 % of the total variation with PC1 (54.92%) and PC2 (23.98%), respectively. The samples with accesulfame K(AF) and honey, which showed high values in moisture level, springiness and sweet taste, showed similar attributes which led to a positive direction of PC1. The correlation between the sensory attributes and consumer acceptance showed that the most important factors for high consumer acceptance were moistness, springiness, sweet taste and sweet flavor. Overall, the samples with accesulfame K(AF) had the closest position in the PLSR results with highest overall consumer satisfaction.

      • KCI등재

        진폐가족의 사회적지지 특성

        박영미,이성은,Park, Young-Mi,Yi, Sung-Eun 한국재활간호학회 2004 재활간호학회지 Vol.7 No.2

        Purpose: The study has planned to find out the perceived social support of the families with pneumoconiosis patients. Method: The subjects of the study were the 300 family care givers of the pneumoconiosis patients who were hospitalized in Taeback, Donghae and Jeongsun Occupational Medical Center. The Social Support Survey Instrument developed by Park(1985) was adopted. Results: The Direct Perceived Supports showed statistically differences by the age(F=1.70 p=0.01) and the state of the disease(F=3.09 p=0.027) of the patients. The Health Situation Centered Support was different by the marietal situation(F=2.29 p=0.48) of the pneumoconiosis patients. The Indirect Perceived Supports were statistically different by sex(t=3.76 p=0.043) and relation with the patient (F=2.49 p=0.048), group joining(t=3.79 p=0.042) of the family care givers. The DPSs were statistically different by family income(F=2.25 p=0.025), family authority(F=2.81 p=0.031) and health insurance status(F=2.13 p=0.026). Recommendation: It is recommended to develop an active social support program at the pneumoconiosis care centers for the middle aged female family care givers of the pneumoconiosis patients with the support of Ministry of Labor, Ministry of Health and Welfare and other NGOs of pneumoconiosis.

      • KCI등재

        치는 시간을 달리한 건식 쌀가루 인절미의 품질특성

        박영미,윤혜현,Park, Young Mi,Yoon, Hye Hyun 한국식품조리과학회 2016 한국식품조리과학회지 Vol.32 No.2

        Purpose: The objective of this study was to investigate the quality characteristics of injulmi made with dry glutinous rice flour according to the punching time (1, 4, 7, 10 min) and the frozen storage period (0, 1, 7, 30 days) as compared with the control made by the traditional method. Methods: Moisture content, texture profile, and sensory evaluation were measured in triplicate and color was measured five times. Results: The moisture content was highest in the sample prepared with 7 min of punching time and the control. As the punching time for injulmi increased, moisture content tended to increase. The L-value increased with increased punching time and the L-value was highest in the samples with 5 min and 7 min of punching time whereas the L-value showed no constant trend with frozen storage. The texture profile analysis indicated that adhesiveness, and gumminess decreased significantly with increased punching time, and punching for 7 min resulted in the highest hardness. The results of an acceptance test, showed that the injulmi prepared with dry glutinous rice flour had higher scores for color, flavor, and taste, texture than the control made with the traditional method. The whiteness of injulmi prepared with dry glutinous rice flour increased with longer punching times and, the sample with 10 min punching time and 7 days of frozen storage had the highest score in the acceptance test. Conclusion: These results can be used as primary data for research on the texture of injulmi made with dry glutinous rice flour.

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