Efficient Interleukin-21 Production by Optimization of Codon and Signal Peptide in Chinese Hamster Ovarian Cells

( Hee Jun Cho ),( Byung Moo Oh ),( Jong-tae Kim ),( Jeewon Lim ),( Sang Yoon Park ),( Yo Sep Hwang ),( Kyoung Eun Baek ),( Bo-yeon Kim ),( Inpyo Choi ),( Hee Gu Lee ) 한국미생물 · 생명공학회 2019 Journal of microbiology and biotechnology Vol.29 No.2

Interleukin-21 is a common γ-chain cytokine that controls the immune responses of B cells, T cells, and natural killer cells. Targeting IL-21 to strengthen the immune system is promising for the development of vaccines as well as anti-infection and anti-tumor therapies. However, the practical application of IL-21 is limited by the high production cost. In this study, we improved IL-21 production by codon optimization and selection of appropriate signal peptide in CHO-K1 cells. Codon-optimized or non-optimized human IL-21 was stably transfected into CHO-K1 cells. IL-21 expression was 10-fold higher for codon-optimized than non-optimized IL-21. We fused five different signal peptides to codon-optimized mature IL-21 and evaluated their effect on IL-21 production. The best result (a 3-fold increase) was obtained using a signal peptide derived from human azurocidin. Furthermore, codon-optimized IL-21 containing the azurocidin signal peptide promoted IFN-γ secretion and STAT3 phosphorylation in NK-92 cells similar to codon-optimized IL-21 containing original signal peptide. Collectively, these results indicate that codon optimization and azurocidin signal peptides provide an efficient approach for the high-level production of IL-21 as a biopharmaceutical.

두가지 혈류 유발방법에 의한 새로운 심폐소생술(이중 혈류 유발 심폐소생술)장치의 개발

황성오,김현,조준휘,오범진,임종천,최경훈,윤정한,이승환,김영식,이강현,이윤선 大韓應急醫學會 1999 대한응급의학회지 Vol.10 No.2

Background: There have been many efforts to augment blood flow during cardiopulmonary resuscitation. These efforts have focused on maximizing the effect of cardiac pump or thoracic pump alone. However, considering that the heart is the biggest blood reservoir and increase of intrathoracic pressure can generate blood flow, simultaneous exploitation of both mechanisms may have synergistic effect. We hypothesized that simultaneous chest constriction in addition to sternal compression by standard CPR may have additive hemodynamic effects by preventing deformation of the chest and increase of intrathoracic pressure. Methods and results: we built a new mechanical device to perform compression and thoracic constriction simultaneously. The device consists of two main elements. Piston in the center is to depress the sternum. Strap is to constrict the thorax circumferentially. Strap is attached to both sides of the piston. When the piston is pushed down, it depresses the sternum and pulls on the thoracic strap. To determine strap width to produce optimal hemodynamic effect, we measured hemodynamic parameters with variable widths of strap in two dogs after induction of ventricular fibrillation. Result of the experiment showed that 10cm wide strap was determined to be most effective. We also determined optimal depth of compression to produce maximal hemodynamic effect with animal experiments using two dogs. Animal experiments showed that the highest aortic pressure could be generated when the stemum was depressed to 5 cm. Cardiopulmonary resusciation using a new device could generate higher systolic aortic pressure, coronary perfusion pressure and end-tidal carbon dioxide tension in comparison with standard cardiopulmonary resuscitation in a pilot animal study using two dogs. Conclusion: New cardiopulmonary resuscitation method using a mechanical device designed by us could perform sternal compression and simultaneous thoracic constriction, and generate better hemodynamic effects than standard cardiopulmonary resuscitation in pilot animal experiments.

서울의 Penicillinase Producing Neisseria Gonorrhoeae 발생빈도(1996)

김재홍,황동규,전재홍,김윤석,김중환,김용준,이창균,임동진,김현수,조창근,김경문,박상훈,전우형,김희성,이호정,차명수,김갑형,김형석,김석우,황지환,박병순,권오상,이민수,송기훈,성소영,이인섭,부태성 대한화학요법학회 1999 대한화학요법학회지 Vol.17 No.2

Background : In recent years, gonorrhea has been panedemic and remains one of the most commom STDs in the world, especially in developing countries. Objective & Methods: For the detection of a more effective therapeutic regimen and assessing the prevalence of PPNG, we have been trying to study the patients who have visited the VD Clinic of Choong-Ku Public Health Center in Seoul since 1980 by means of the chromogenic cephalosporin method. Results: In 1996, 139 strains of N. gonorrhoeae were isolated, among which 53(39.0%) were PPNG. Conclusion: Our results suggests that after a peak of 74.3% in 1993, the prevalence of PPNG in Seoul is gradually declining.

알레르기 환자에서 MAST-CLA 검사의 의의

남해선,황규윤,,백병준,정윤영,오천환,조성란,박준수 순천향의학연구소;Soonchunhyang Medical Research Institute 2000 Journal of Soonchunhyang Medical Science Vol.6 No.1

To evaluate usefulness of application of MAST-CLA to indetify allergens, authors conducted MAST-CLA allergen-specific IgE assay and collected allergy related symptoms in 118 allergic patients diagnosed at Soonchunhyang University Chunan Hospital during October 1998 through January 2000. The results were as followings; 1. The mean (SD) age was 23.1 (±12.17) years old and teenagers accounted for 33.1% of patients. 2. The frequent allergic symptoms were sneezing (59.3%), rhinorrhea (53.4%), nasal stiffness (44.1%), itching sensation (24.6%), cough or sputum (16.1%), and eye symptom (8.5%) in order. 3. Among these subjects, 62.7% of patients revealed at least more than one specific allergen and 70.3% showed elevated total IgE levels by MAST-CLA. The three most frequent specific allergens were Dermatophagoides farinae (47.5%), Dermatophagoides pteronyssinus (45.8%), and house dust (30.5%). 4. While age was not significantly related to positive of both specific allergens and total IgE (P>0.05), sneezing and nasal stiffness were significantly associated with positive of specific allergen and total IgE, respectively (both P<0.05). 5. Total IgE increased with the number of specific allergens, but both specific allergen and total IgE were not significantly related to increases of allergic symptoms (P>0.05). In conclusion, our data suggest that MAST-CLA allergen-specific IgE assay is useful measure for identification of causative allergens in allergic patients, however, it is recommended that more specific allergic antigens should be determined and replaced or added to Korean inhalent panel of MAST-CLA.

網膜色素上皮細胞의 Lysosomal acid phosphatase의 活性値 測定

김복희,김은경,김윤화,백미화,이지숙,조영숙,황응숙 曉星女子大學校 藥學大學 學生會 1988 曉星藥誌 Vol.4 No.-

Measurement of lysosomal enzyme activity in the rabbit eye was effected using acid phosphatase as enzyme markers. Acid phosphatase in the retinal pigment epithelium of the rabbit eye was studied using standard enzymological techniques. The enzyme activity in the lysosomal extract of the pigment epithelium was 54.9±23.0 King-Armstrong Unit/dl.

Lab-on-a-Disc for Simultaneous Determination of Nutrients in Water

Hwang, Hyundoo,Kim, Yubin,Cho, Juhye,Lee, Ji-yoon,Choi, Man-Sik,Cho, Yoon-Kyoung American Chemical Society 2013 ANALYTICAL CHEMISTRY - Vol.85 No.5

<P>In this study, we describe a novel platform based on centrifugal microfluidics for simultaneous determination of nitrite, nitrate and nitrite, ammonium, orthophosphate, and silicate in water samples. All processes from sample metering to detection were integrated and automatically conducted on a rotating disc-shaped device. Fluid transfer was controlled by laser irradiation on the ferrowax-based microvalves. Liquid samples and reagents were pumped by centrifugal force in the rotating disc, and their positions and movements were controlled through a programmable light from a laser diode. This novel water analysis platform required only 500 μL of sample (100 μL for each nutrient) and 10–30 μL of reagents for colorimetric detection. In addition, the fully automated parallel processes and efficient mixing in the rotating disc allowed for a significant reduction in total analysis time (∼7 min 40 s) and increased accuracy. Validation with a seawater certified reference material indicated that the platform accurately measured nutrient concentrations in water samples. In addition, we showed that the nutrients in the seawater collected from Chunsu Bay in Korea measured by the proposed lab-on-a-disc and by a commercialized autoanalyzer are comparable.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/ancham/2013/ancham.2013.85.issue-5/ac3036734/production/images/medium/ac-2012-036734_0005.gif'></P>

Clinical Significance of Chronic Kidney Disease and Atrial Fibrillation on Morbidity and Mortality in Patients with Acute Myocardial Infarction

Hwang, Hyeon Seok,Park, Mahn-Won,Yoon, Hye Eun,Chang, Yoon Kyung,Yang, Chul Woo,Kim, Suk Young,Cho, Jung Sun,Kim, Chan Joon,Park, Gyung-Min,Park, Chul-Soo,Choi, Yun-Seok,Koh, Yoon-Seok,Lee, Jong Min,S S. Karger AG 2014 American journal of nephrology Vol.40 No.4

<P>Abstract</P><P><B><I>Background/Aims:</I></B> Atrial fibrillation (AF) often coexists with acute myocardial infarction (AMI), and chronic kidney disease (CKD) is a major risk for AMI. However, the combined impact of CKD and AF on the mortality and morbidity in AMI population has not been determined. <B><I>Methods:</I></B> Between January 2004 and December 2009, a total of 4,738 AMI patients were enrolled prospectively. Patients were divided into four groups according to the combined status of CKD and AF. The primary endpoint was a combination of 5-year major adverse cardiac and cerebrovascular events (MACCE). <B><I>Results:</I></B> The prevalence of AF was significantly higher in CKD patients than in non-CKD patients (6.76 vs. 3.31%, p < 0.001). The highest cumulative event rate of MACCE and death was observed in patients with both CKD and AF (68.5 and 64.0%), respectively. In multivariable analyses, compared with patients with neither AF nor CKD, hazard ratios (HR) for composite of MACCE were 1.66 (95% CI, 1.14-2.41), 1.24 (95% CI, 1.06-1.46), and 2.10 (95% CI, 1.42-3.13) for patients with AF only, those with CKD only, and those with both CKD and AF, respectively (p for interaction = 0.935). Patients with both CKD and AF had a greatest risk for all-cause mortality (HR 2.54; 95% CI, 1.60-4.53), and the significant synergistic interaction was observed between CKD and AF (p for interaction = 0.015). <B><I>Conclusion:</I></B> The combined effect of AF and CKD on the risk of MACCE after an AMI is stronger than any separate condition, and it confers a synergistic effect on the all-cause mortality risk.</P><P>© 2014 S. Karger AG, Basel</P>

Electrochemical Properties of Li Metal Batteries with P(PEGMA)-Coated Lithium Trivanadate Cathode and Li Powder Anode

Cho, Sung Ho,Hwang, Sun Woo,Kim, Byung Hyuk,Bae, Ki Yoon,Yoon, Hyun,Yoon, Sam S.,Yoon, Woo Young American Scientific Publishers 2016 Journal of Nanoscience and Nanotechnology Vol.16 No.10

<P>Ionic conductive poly(poly(ethylene glycol) methyl ether methacrylate) (hereafter polymer) was coated onto a lithium trivanadate (LiV3O8) cathode by using an electrostatic spray deposition method in an effort to improve the ionic conductivity and prevent dissolution of vanadium into the electrolyte. The coating layer on the LiV3O8 electrode was identified by Fourier transform infrared spectroscopy, field emission scanning electron microscopy and transmission electron microscopy. Polymer was uniformly coated on the LiV3O8 electrode with a thickness of about 40 nm. Lithium powder, synthesized by the droplet emulsion technique, was used as the anode. Electrochemical charge-discharge tests showed that the cell based on the polymer-coated LiV3O8 cell exhibited an initial specific capacity of 186 mAh/g and the capacity retentions of the polymer-coated LiV3O8 after 100cycles was 87%, which is far improved that of uncoated cell (65%). Also, the coated cell showed 81% of the capacity retention after 300 cycles at 2 C-rate. These results demonstrate that the polymer coating on LiV3O8 is effective for suppressing capacity fading at high C-rate.</P>

Toxicogenomics Study on ${\alpha}-Naphthylisothiocyanate\;(ANIT)$ Induced Hepatotoxictiy in Mice

Hwang, Ji-Yoon,Lim, Jung-Sun,Jeong, Sun-Young,Park, Han-Jin,Cho, Jae-Woo,Yoon, Seok-Joo The Korean Society of Toxicogenomics and Toxicopro 2006 Molecular & cellular toxicology Vol.2 No.1

[ ${\alpha}-Naphthylisothiocyanate$ ] (ANIT) induces intrahepatic cholestasis, involving damage to biliary epitheial cells. This study investigates hepatic gene expression and histopathological alterations in response to ANIT treatment in order to elucidate early time response of ANIT-induced hepatotoxicity. ANIT was treated with single dose (3, 6, and 60 mg/kg) in corn oil by oral gavage. Serum biochemical and histopathological observation were performed for evaluation of hepatotoxicity level. Affymetrix oligo DNA chips were used for gene expression profile by ANIT-induced hetpatoxicity. Hepatic enzyme levels (ALT, AST, and ALP) were increased in 24 hr high dose group. In microscopic observations, moderate hepatocellular necrosis, were confirmed 24 hr high dose groups. We found that gene expression patterns were dependent on time and dose. Our selected genes were related inflammation and immunomodulation. In this study, ANIT-induced hepatotoxicity was involved in acute phase responses and provides evidence for role of neutrophil could be mechanism associated with ANIT-mediated hepatotoxicity.

Material Flow Analysis of Mercury Contained in Paint and Pigment

( Yoon-a Cho ),( Mi-jeong Jeong ),( Tea-wan Jeon ),( Dong-gun Hwang ),( Hee-sung Lee ),( Jin-mo Yeon ),( Yong-jun Kim ),( Na-hyeon Cho ) 한국폐기물자원순환학회(구 한국폐기물학회) 2015 한국폐기물자원순환학회 3RINCs초록집 Vol.2015 No.-

In order to efficiently fulfill the agreement Minamata convention, Path to the environment from mercury emissions from the production of mercury should be aware of the environment of mercury flow through the integrated management of the entire process. In this study, Were selected for the paint(pigment) in consideration of the priority by looking at the mercury material flows from production to disposal, we want to create a step-based materials such as Minamata Convention to respond to international treaties. Analysis of the mercury content of the sample 76 except the waste water, Mercury is detected in the 0 ~ 0.25 mg / kg level had a very low level as compared with the mercury content based paint for children 25 mg / kg of the United States. The annual mercury emissions in paint products produced is 0.67 kg, Being in the domestic distribution of a 0.63 kg / year. Mercury pigments detected 10 of 11 products, paint has been investigated as one. The reason is low detection rate in the paint product is determined by the dilution due to the pigment while being mixed with a large amount of solvent. When compared to the amount of mercury treatment in this study and in previous studies that the amount of mercury put paint(pigment), The mercury emissions from existing literature 18 times higher, In accordance with the regulations, such as RoHS mercury-containing products to the existing paint is considered to be due to decreased As eradicated.