Ethanol Extract of Ganoderma lucidum Augments Cellular Anti-oxidant Defense through Activation of Nrf2/HO-1

Lee, Yoo-hwan,Kim, Jung-hee,Song, Choon-ho,Jang, Kyung-jeon,kim, Cheol-hong,Kang, Ji-Sook,Choi, Yung-hyun,Yoon, Hyun-Min KOREAN PHARMACOPUNCTURE INSTITUTE 2016 Journal of pharmacopuncture Vol.19 No.1

Objectives: The mushroom Ganoderma lucidum has been widely used as a traditional herbal medicine for many years. Although several studies have focused on the anti-oxidative activity of this mushroom, the molecular mechanisms underlying its activity have not yet been clearly established. The present study investigated the cytoprotective effect of ethanol extract of Ganoderma lucidum (EGL) against oxidative stress (hydrogen peroxide, $H_2O_2$) and elucidated the underlying mechanisms in a C2C12 myoblast cell line. Methods: Oxidative stress markers were determined by using the comet assay to measure reactive oxygen species (ROS) generation and deoxyribonucleic acid (DNA) damage. Cell viability and Western blotting analyses were employed to evaluate the cellular response to EGL and $H_2O_2$ in C2C12 cells. Transfection with nuclear factor erythroid 2-related factor 2 (Nrf2)-specific small interfering ribonucleic acid (siRNA) was conducted to understand the relationship between Nrf2 expression and $H_2O_2$-induced growth inhibition. Results: The results showed that EGL effectively inhibited $H_2O_2$-induced growth and the generation of ROS. EGL markedly suppressed $H_2O_2$-induced comet-like DNA formation and phosphorylation of histone H2AX at serine 139 ($p-{\gamma}H2AX$), a widely used marker of DNA damage, suggesting that EGL prevented $H_2O_2$-induced DNA damage. Furthermore, the EGL treatment effectively induced the expression of Nrf2, as well as heme oxygenase-1 (HO-1), with parallel phosphorylation and nuclear translocation of Nrf2 in the C2C12 myoblasts. However, zinc protoporphyrin IX, a HO-1 inhibitor, significantly abolished the protective effects of EGL against $H_2O_2$-induced accumulation of ROS and reduced cell growth. Notably, transient transfection with Nrf2-specific siRNA attenuated the cytoprotective effects and HO-1 induction by EGL, indicating that EGL induced the expression of HO-1 in an Nrf2-dependent manner. Conclusion: Collectively, these results demonstrate that EGL augments the cellular anti-oxidant defense capacity through activation of Nrf2/HO-1, thereby protecting C2C12 myoblasts from $H_2O_2$-induced oxidative cytotoxicity.

어린 브리티쉬 숏헤어 고양이에서 발생한 비대성심근병증

임수정,이선희,손선호,최호정,송근호 忠南大學校 獸醫科大學 附設 動物醫科學硏究所 2008 動物醫科學硏究誌 Vol.15 No.1

A l0-month-old, neutered male, British shorthair cat was referred to the Veterinary Medical Teaching Hospital of Chungnam National University with the history of tachypnea, anorexia and depression which onset were two days ago. On physical examination, increased respiratory rate and no murmur were found. A complete blood count indicated thrombocytosis. Results from a serum biochemical profile demonstrated elevations in CK, ALP, BUN and Ca, and decreases in sodium, potassium and chloride. Thoracic radiographs revealed patchy interstitial opacity increase and left atrium enlargement. Echocardiographs showed the evidences of hypertrophic cardiomyopathy which were hypertrophy of diastolic interventricular septum and left ventricular free wall. Other findings on echocardiography were the reverse E:A ratio(tranmitra1 inflow pattern) and absence of systolic anterior motion of mitral valve. Electrocardiographs demonstrated wide P and R wave, which mean left atrium enlargement and left bundle branch block, respectively. Medications including furosemide (2mg/kg), enalapril (0.25mg/kg), and spironolactone (1mg/kg) were prescribed twice a day and orally. Client education about exercise restriction and possibility of aggressive progress was given. Clinical signs suggestive of thromboembolism were developed on 6th day after showing tachypnea; right forelimb lameness, bilateral hindlimb lameness, loss of femoral pulse and severe pain. After all, euthanasia was performed at local animal hospital, according to the owner's request.

청소년의 스마트폰 중독과 우울 및 불안과의 관계

김경은,김필송,민주연,박수경,신서인,이지은,정해인,조호정,최정원,최정인 이화여자대학교 간호과학대학 2013 이화간호학회지 Vol.- No.47

Purpose: The purpose of this study was to investigate the relationships between the degree of smart phone addiction among adolescents and their depression and anxiety levels. Method: In this study, 379 high school students were selected using the convenient sampling method. The instruments used for this study were smart phone addiction measure, the CES-D (Center for Epidemiological Studies-Depression Scale), and the STAI (State-Trait Anxiety Inventory). Data were analyzed using the SPSS 20.0 program with descriptive statistics, t-test, ANOVA with Scheffe test, and Pearson’s correlation coefficient. Result: The mean score for smart phone addiction was 40.45±17.27, depression was 36.56±9.58, state anxiety was 44.00±9.61, and trait anxiety was 45.70±9.75. There were significant differences between the degree of smart phone addiction and the following variables: gender (t=-4.953, p<.001), hours of smart phone use per day (F=12.259, p<.001), types of frequently used features (F=3.485, p=.008), and satisfaction level for smart phone (F=5.18, p<.001). There were statistically significant relationships (p<.001) among degree of smart phone addiction, depression level, and state and trait anxiety levels. Conclusion: The results of this study suggested that there was a significant relationship between smart phone addiction and mental health, specifically, depression and anxiety among adolescents. Further research is needed to develop nursing strategies to provide care for adolescents who frequently utilize smart phones.

복합부위 통증증후군 1형에서의 Ropivacaine, Ketamine, Clonidine을 이용한 정맥부위 차단의 효과 : 증례 보고 A case report

송호경,이진영,정성우 대한마취과학회 2002 Korean Journal of Anesthesiology Vol.43 No.6

There is no treatment either to prevent the development of complex regional pain syndromes (CRPS), or to adequately and specifically control established pain. Symptom-based analysis of pain is important for disease progress assessments and treatment outcomes. An intravenous regional block (IVRB) with local anesthetic can contribute to the management of CRPS. We observed that by the IVRB using ropivacaine, ketamine and clonidine, the patient with CRPS type Ⅰ who was resistant to sympathetic ganglion block and medical treatment with gabapentin, carbamazepine and antidepressant, was relieved of neuropathic pain. (Korean J Anesthesiol 2002; 43: 810~814)

백서의 복강내에 투여한 Mitomycin-C-Polyglycolic acid 제형이 장기에 미치는 영향에 관한 연구

송규상,강대영,최정목,노승무,정경수,오정연,김진향,양준목,최선웅,이진호,조준식,민병무,김용백,김창식,박근성,김승영,김학용,인현빈 충남대학교 의과대학 지역사회의학연구소 1998 충남의대잡지 Vol.25 No.1

To evaluate the in-vivo effects of mitomycin-C-polyglycolic acid composite(MMC-PGA) the disk: shaped composite were implanted into the peritoneal cavity of the Sprague-Dawley rats. The pathologic changes were examined at various time points up to 12 weeks. Initially the soft tissue around the inserted disks showed capillary congestion at 3 days. Inflammatory infiltrates with foreign body giant cells appeared from the 2nd week and reached peak response at 6-8 weeks. These reaction diminished prominently at 12 weeks. No specific pathologic change was found in the liver, the kidneys, and the heart. The above results suggest that the MMC-PGA composite can serve as a new device for intraperitoneal chemotherapy of various types of cancers.

강관 조립 틀비계의 성능 분석

송길호,곽순섭,김호수,정성진 대한건축학회 2003 대한건축학회 학술발표대회 논문집 - 계획계/구조계 Vol.23 No.2(구조계)

About the steel pipe built-up scaffolding(BTS), which is being used in domestic construction sites, there are no related codes to the overall capacity of the BTS in the Industrial Safety and Health Act (ISHA) but only the ones which describe the testing method and the capacity of the each member of BTS. So we need to know the full capacity of the BTS at the time of the being used stage, not the ones of the members but the one of the total BTS frame. In this study, first, the vertical frame member which is the basic element of the BTS frame, is tested for its capacity. Second, the load test about the full strength of the BTS system are performed in two ways namely, for the first-story and the second-story BTS frame. In each case, the centric load(1/2 L) test and the eccentric load(1/4 L, 0L) tests are carried out, where L is the longer length of the BTS frame. According to the results of the experiments, first, the load endurance capacity of the first-story BTS frame are larger than the one of the second-story BTS frame at the same conditions. Second, the centric load endurance capacity is bigger than the one of the eccentric loading in the same story BTS system structure.

백서 하악골에서 신연빈도에 따른 골형성 비교 연구

송정호,신상훈,김종렬 대한악안면성형재건외과학회 2002 Maxillofacial Plastic Reconstructive Surgery Vol.24 No.2

The purpose of this study was to investigate the clinical, radiographic, histologic and molecular changes in distraction osteogenesis according to different distraction rhythm in the rat's mandible. Forty-five adult male rats underwent open osteotomy between the second and third molars and external unilateral distraction device was applied. Latency was allowed for 5 days before distraction began. The distraction device was activated with same rate of 0.5㎜ per day and varying distraction rhythms of once daily, twice daily, four times daily until 3㎜ length gain was achieved. Tissues were harvested after 4 days, 2 weeks, and 4 weeks after distraction for Western blot analysis, RT-PCR analysis and harvested 3 weeks, 6 weeks after distraction for clinical, radiographic, and histologic studies. Three animals were killed at each time point. The results obtained from this study were as follows: No significant difference was found on clinical examination according to distraction rhythms, and increasing the frequency of distradction was shown to improve regenerate bone formation on radiographic and histologic examination, and TGF-β1 expression increased when small, more frequent distractions were performed. From these results, it could be stated that more frequent distraction is shown to significantly improve and accelerate bone formation and has considerable effects on TGF-β1 expression.

HEMT소자의 전송특성 해석을 위한 채널내의 산란율에 관한 양자역학적 연구

송영진,도태영,황호정 중앙대학교 기술과학연구소 1994 기술과학연구소 논문집 Vol.24 No.-

In this paper the rates of two-and three-dimensional scattering are calculated in pseudomorphic ??/??/GaAs HEMT heterostructure systems. The electronic states and wave functions in the square quantum well are determined numerically by the self-consistent solution of Poisson's and Schrodinger's equations. The wave functions and energy levels obtained are used to calculate the major two-dimensional scattering rates in this structure. Polar optical- phonon and acoustic- phonon, piezoelectric, ionized impurity and alloy scattering rates are considered for the first two subbands. This results are compared to the three-dimensional scattering rates also calculated using by the triangular approximation of quantum well in the same structure.

스케일이 있는 급수관내의 유도초음파의 전파 특성에 관한 연구

송성진,김영환,이동훈,이현동,배철호,박정훈 한국비파괴검사학회 2003 한국비파괴검사학회지 Vol.23 No.1

배관 내의 스케일은 통수비를 감소시키므로 배관을 적절히 관리하기 위해서는 스케일량에 대한 정량적인 평가가 필수적이다. 상수도 급수관내의 스케일량을 평가하기 위해서 유도초음파를 사용하였다. 가변각 웻지를 사용하여 유도 초음파의 여러 모드를 발생시키고, 노후 배관을 따라서 전파하는 특성을 분석하였다. 실험적으로 관내의 스케일이 증가함에 따라서 f×d=1.5㎒·㎜인 경우에 F(M,2) 모드들의 진폭이 크게 감소하는 것을 알 수 있었다. 본 연구의 결과로 상수도 급수관의 스케일량 평가에 적합한 유도 초음파는 F(M,2) 모드들임을 알 수 있었다. Since the scale in pipes reduces the flow rate, a quantitative evaluation of the scale is essential for the proper maintenance of pipes. Guided waves were employed to estimate the amount of scale in water supplying pipes. Using variable angle wedge, several modes of guided waves were generated and their propagation charcteristics along the pipes with scale were analyzed. It was experimentally observed that the amplitude of F(M,2) modes at f×d=1.5㎒·㎜ decreased significantly with increasing amount of scale. The present study showed that F(M,2) modes were optimal to evaluate the scale in water supplying pipes.

치과기공사의 작업환경 개선 설비현황과 업무 스트레스 관련 연구

정인호,신중규,이송권 慶山大學校 保健福祉硏究所 2003 保健福祉硏究 Vol.8 No.-

The purpose of this study was to find out a scheme to reduce a job stress of dental technicians and to increase and operation efficiency with the improvement of working environment. The method of study was by the questionnaire-survey from dental technicians working in the dental clinics or the dental department of clinics or hospitals in DaeguㆍKyungbuk, for the period of Jul 1 - Aug 2, 2002. Here 246 ones answered relative faithfully among 300 questionnaires were used. The results of study were as follows; 1. In the stress degree of dental technicians, stress by work load, job conflict, and pay showed significant results in crown jobs(p<0.001), long-time workers(p<0.0001) and discontinuing workers(p<0002), and technicians(p<0.007), respectively. 2. From looking over facility for improvement of working environment conditions of dental technicians, it's found that most of illumination and noise protection facilities and a part of respiratory protection facilities are not installed for dental technicians satisfactorily. 3. In relationship between installation for work environment improvement and stress, technicians who worked in good environment condition had lower stress degree than workers with unsatisfied work enrironment in most of all characteristics. Especially the result showed that installation for respiratory system is very important for reduction of factors causing stresses.