Characterization of the European type of maternal lineage evident in extant Jeju native pigs

Byeong‐Woo Kim,In‐Cheol Cho,Moon‐Sung Park,Tao Zhong,임현태,Sung‐Soo Lee,Hee‐Bok Park,Moon‐Suck Ko,Jun‐Heon Lee,Jin‐Tae Jeon 한국유전학회 2011 Genes & Genomics Vol.33 No.2

Using a partial D‐loop sequence of mtDNA, an intensive analysis was conducted of the maternal lineages of Jeju native pigs (JNPs) from Korea. In total, 100 mtDNA sequences were obtained from Asian wild boars (AWBs), European wild boars (EWBs), Asian domestic pigs (ADPs), European domestic pigs (EDPs), and JNPs and were used for phylogeny and network analyses. Two distinct JNP groups - one (JNPA) in the Asian cluster and the other (JNPE) in the European cluster - were identified in the estimated phylogenetic tree and network. The maternal lineage of JNPE was the closest to that of EWB and a clear haplogroup was identified that shared an identical haplotype (hap04) among 15 individuals of JNPE and 2 individuals of EWB. A Landrace and an EWB shared hap03with a JNPE. EWB, Landrace, Large White, and Duroc formed two clear haplogroups with JNPE in a parsimonious medianjoining network analysis, suggesting that an obvious maternal contribution of EDP has occurred in JNPE in recent years. A pair‐wise mismatch analysis also indicated that JNPE may have experienced a sudden population expansion, suggesting a more recent establishment compared with the gradual population expansion of JNPA. The JNPE group therefore should be further evaluated in order to decide whether this group should be culled or accepted into further programs for maintenance of the JNP population as a pure breed.

Differential Manifestation of Ocular Phenotypes in TALEN-mediated p19arf Knockout FVB/N and C57BL/6J mouse lines

Jin‑Sung Park,Joo‑Il Kim,Hyun‑Jin Lim,Soo‑Kyung Ryu,Euna Kwon,Kang‑Min Han,Ki‑Taek Nam,Han‑Woong Lee,Byeong‑Cheol Kang 한국유전학회 2020 Genes & Genomics Vol.42 No.9

Background p19arf, primarily known as a tumor suppressor, has also been reported to play an essential role in normal development of mouse eyes. Consistently, lack of p19arf has been associated with ocular defects, but the mixed background of the knockout (KO) mouse strain used raised a concern on the accuracy of the phenotypes observed in association with the targeted gene due to genetic heterogeneity. Object We carried out a study to investigate into the efect of genetic background on the manifestation of p19arf KO associated phenotypes. Methods We characterized the phenotypes of novel p19arf KO mouse lines generated in FVB/N and C57BL/6J using a transcription activator-like efector nuclease (TALEN) system in comparison to the reported phenotypes of three other p19arf-defcient mouse lines generated using homologous recombination. Results Ninety-fve percent of FVB/N-p19arf KO mice showed ocular opacity from week 4 after birth which worsened rapidly until week 6, while such abnormality was absent in C57BL/6J-p19arf KO mice up to the age of 26 weeks. Histopathological analysis revealed retrolental masses and dysplasia in the retinal layer in FVB/N-p19arf KO mice from week 4. Besides these, both strains developed normally from birth to week 26 without increased tumorigenesis except for a subcutaneous tumor found in a C57BL/6J-p19arf KO mouse. Conclusion Our fndings demonstrated surprisingly variable manifestation of p19arf-linked phenotypes between FVB/N and C57BL/6J mice, and furthermore between our mouse lines and the established lines, indicating a critical impact of genetic background on functional study of genes using gene targeting strategies in mice.

진위천 유역 수질 특성 분석

박병기 ( Byeong Ky Park ),김진혁 ( Jin Yuk Kim ),이동준 ( Dong Jun Lee ),신경용 ( Kyung Yong Shin ),박민지 ( Min Ji Park ),문정숙 ( Jeong Suk Moon ),이영준 ( Young Joon Lee ),이재관 ( Jae Kwan Lee ) 한국물환경학회(구 한국수질보전학회) 2014 한국물환경학회·대한상하수도학회 공동 춘계학술발표회 Vol.2014 No.-

구성주의적 작문 수업이 아동의 작문수행에 미치는 효과

강병재(Byeong Jae Kang),김혜진(Hye Jin Kim) 한국아동학회 2000 兒童學會誌 Vol.21 No.2

Ninety 6th graders were randomly assigned to an experimental or a control group in this 5 week study of the effects of constructivist instruction on writing performance. After the writing pre-test, the experimental group was treated with constructivist instruction while the control group was treated with a tradition procedure based on Joyce and Weil`s(1992) basic exercise model Instruction consisted of ten 40-minute sessions. The effectiveness of the constructivist instruction was tested by post-and retention-tests. Two raters scored the children`s writing by the analytical scale of Jin-Suk Won(1994). Results were analyzed by t-test. The writing performance and the retention scores of the experimental group were higher than that of the control group. The results of the sub-criteria scores showed significant effects on children`s understanding of contents and constructive performance.

Bupivacaine과 ropivacaine이 Xenopus oocyte에 발현된 HERG 전류에 미치는 영향

김국성,이규승,김효신,손숙진,이상도,김광진,전병화,김윤희,박진봉 충남대학교 의과대학 의학연구소 2003 충남의대잡지 Vol.30 No.1

Bupivacaine is an amide-type local anesthetic widely used for regional anesthesia. Ropivacaine is developed as a less cardiotoxic alternatives to bupivacaine. In the present study, we have analyzed the effects of bupivacaine and ropivacaine on HERG currents expressed in Xenopus oocytes. Bupivacaine and ropivacaine(3∼1,000μM) blocked HERG currents in a concentration dependent manner. EC_(50) was 26.1±3.1μM(n_(R) 0.65±0.04) and 43.5±7.9μM(n_(H) 0.99±0.13) in bupivacaine and ropivacaine, respectively. Bupivacaine and ropivacaine did not affect the activation and deactivation kinetics of HERG channels. However, the drugs decreased the slope conductance measured from fully activated current-voltage relationship curves. These results suggest that bupivacaine and ropivacaine have a similarinhibitory effect on HERG channels, which could be a possible cellular mechanism of LQT or ventricular arrythmia by the drugs.

정신분열병에 대한 리스페리돈의 효과 및 안정성

이민수,김용구,김영훈,연병길,오병훈,윤도준,윤진상,이철,정희연,강병조,김광수,김동언,김명정,김상훈,김희철,나철,노승호,민경준,박기창,박두병,백기청,백인호,손봉기,손진욱,양병환,양창국,우행원,이정호,이종범,이홍식,임기영,전태연,정영조,정영철,정인과,정인원,지익성,채정호,한상익,한선호,한진희,서광윤 大韓神經精神醫學會 1998 신경정신의학 Vol.37 No.1

연구목적 : 본 시험의 목적은 임상시험 시작전에 연구자들을 대상으로 PANSS Workshop을 통하여 PANSS, ESRS에 대한 국내에서의 표준화 작업을 구축하고 새로운 정신병 치료제인 리스페리돈의 효과와 안정성을 재확인하여 리스페리돈 사용에 대한 적정화를 이루는데 있다. 연구방법 : 1996년 4월부터 1996년 9월까지 국내 39개 대학병원 정신과에 입원중인 혹은 증상이 악화되어 입원하는 정신분열병 환자 377명을 대상으로 다시설 개방 연구를 시행하였다. 1주일간의 약물 배설기간을 가진후, 리스페리돈을 8주간 투여하였고, 기준점, 1주, 2주, 4주, 그리고 8주후에 평가되었다. 용량은 제1일에는 리스페리돈 1mg씩 1일 2회, 제2일에는 2mg씩 1일 2회, 제3∼7일에는 3mg씩 1일 2회 투여하였다. 이후 환자의 임상상태에 따라 임의로 증량할 수 있으며, 최대 일일 16mg을 초과하지 않도록 하였다. 추체외로 증상을 조절하기 위한 투약을 허용하였다. 임상증상 및 부작용의 평가는 PANSS(Positive and Negative Syndrome Scale), CGI(Clinical Global Impression) 그리고 ESRS(Extrapyramidal Symptom Rating Scale)을 사용하였다. 연구결과 : 377명중 343명(91%)이 8주간의 연구를 완결하였다. 치료 종결시점인 8주후 PANSS 총점수가 20% 이상 호전된 경우를 약물 반응군으로 정의할때, 약물반응군은 81.3%였다. 리스페리돈에 반응하는 예측인자로는 발병연령, 이전의 입원 횟수, 유병기간이 관련 있었다. 리스페리돈은 1주후부터 PANSS양성, 음성, 및 일반정신병리 점수상에 유의한 호전을 보여 효과가 빨랐다. CGI의 경우도 기준점에 비해 1주후부터 유의한 감소를 나타내었다. ESRS의 경우, 파킨슨 평가점수는 기준점과 비교해 투여 1주, 2주, 4주후 유의하게 증가되었다가 8주후 기준점과 차이가 없었다. Dystonia 평가점수는 1주후만 유의한 증가를 보였으며, dyskinesia 평가점수는 유의한 차이가 없었다. 혈압, 맥박수의 생명징후 및 일반 혈액학 검사, 생화학적 검사, 심전도 검사에서 유의한 변화는 없었다. 결 론 : 이상의 다시설 개방 임상 연구를 통해 리스페리돈은 정신분열병 환자에서 양성증상뿐만 아니라 음성증상 및 전반적인 증상에도 효과적인 것으로 사료된다. 보다 명확한 평가를 위해서는 다른 항정신병약물과의 이중맹검 연구가 필요할 것으로 생각되며, 또한 장기적 치료에 대한 평가도 함께 이루어져야 하겠다. Objective : The purpose of this study was to investigate the efficacy and safety of risperidone in the treatment of Korean schizophrenic patients. Method : This multicenter open study included 377 schizophrenic patients drawn from 39 university hospitals. After a wash-out period of 1 week, the schizophrenic patients were treated with risperidone for 8 weeks and evaluated at 5 points ; at baseline, and 1, 2, 4 and 8 weeks of treatment. The dose was increased from 2mg/day(1mg twice daily) to 6mg/day(3mg twice daily) during the first week and adjusted to a maximum of 16mg/day over the next 7 weeks according to the patient's clinical response. Medication to control extrapyramidal symptoms was permitted. The psychiatric and neurological status of the patients was assessed by PANSS, CGI, and ESRS scales. Results : 343(91%) of 377 patients completed the 8-week trial period. Clinical improvement, as defined by a 20% or more reduction in total PANSS score at end point, was shown by 81.3% of patients. The predictors of response to risperidone were associated older age, shorter duration of illness, fewer previous hospitalization. Risperidone had rapid onset of action ; a significant decrease of the total PANSS and three PANSS factor(positive, negative, general), and CGI was already noticed at the end of first week. For the ESRS, parkinsonism rating scores were significantly increased until week 4 comparing with baseline. Dystonia rating scores were significantly increased until week 1, and dyskinesia rating scores were not significantly changed during the study. Laboratory parameters including vital sign, EKG, hematological, and biochemical values showed no significant changes during the trial. Conclusions : This study suggests that risperidone is generally safe and effective against both the positive and negative symptoms in our group of patients.

8주간의 걷기운동과 맨발걷기가 중년여성의 혈중 세로토닌, 코티졸, 에피네프린 그리고 노르에피네프린에 미치는 영향

정진숙,김병완,김창환 대전대학교 기초과학연구소 2019 自然科學 Vol.30 No.-

The purpose of this study is to inspect effects of regular walking and barefoot walking, which is the easiest way for middle-aged women to exercise in daily life, on serotonin, cortisol, epinephrine and norepinephrine in blood and probe the differences of hormonal changes between two groups. Both of two programs were performed three times a week and each program lasted for more than 30 minutes. The group of regular walking was set at 40-60%(HRmax) and the group of walking barefoot was set at level 10-12(RPE). This research is for reducing middle-aged women's stress and helping to promote their health. All the data is processed by using the arithmetical mean and standard deviation and two-way ANOVA is used to verify differences between groups and durations. In case that there is interaction, independent t-test is used for group comparison and dependent t-test is used for duration comparison to inspect details. Degree of hypothesis acceptance is set at p<.05, p<.01, p<.00l. Firstly, the serotonin rate is reduced in both groups after conducting the programs. Significant main effect of serotonin has differences related to durations at F(1.18)=8.281, p=.01 but there's no differences between groups at F(1.18)=.289, p=.597. There is no differences in interaction as well at F(1.18)=.432, p=.520. Secondly, significant main effect of the cortisol rate in both groups after conducting the programs has no differences related to durations at F(1,18)=.292, p=.595 and there's no differences between groups at F(1.18)=.121, p=.732. There is no differences in interaction as well at F(1.18)=.899, p=.356. Thirdly, the result of paired t-test shows that epinephrine rate of the group performing regular walking is reduced more than one of those who walk barefoot at t=2.326, df=9, p=.045 and there is a difference in interaction at F(1.18)=5.815, p=.027. Lastly, the norepinephrine rate of those who take usual walks shows a meaningful difference related to durations at F(1.18)=24.505, p=.00l which is more noticeable than one of the other. However, there is no difference between groups at F(1.18)=.175, p=.680 and there is also no difference in interaction at F(1.18)=.902, p=.355. The result says that both of the programs, regular walking and walking barefoot, don't influence the amount of middle-aged women's stress positively, which is against the previous studies. It is because this research exclude other factors which can influence hormone secretion of middle-aged women such as weather, time, diet and mental state in middle age. It can be deduced that future researches to promote middle-aged women's health and decrease the symptoms of depression should include changes caused by temperature, time to exercise, exercise intensity and subject's psychological state in middle age.

Gate array를 이용한 8-bit CMOS Subranging ADC의 설계

권병진,정강민 成均館大學校 科學技術硏究所 1992 論文集 Vol.43 No.1

An 8-bit Subranging Converter(ADC) has been realized in a 1.2-㎛ Silicon Gate, Double Metal CMOS process. Gate arrays have used for the design. The ADC uses 31 comparators and is capable of conversion rates to 6.7MHz at Vdd = 5V, Vss = -5V. A voltage comparator with 4mV resolution and 61ns response time, and analog switch(Transmission Gate) of 300Ω on-resistance value can be realized on the same chip with digital circuits. Chip size is 2.284X2.284㎟

從聯合國席位和核危機看中國對韓半島外交政策

李炳鎭 평택대학교 1998 論文集 Vol.11 No.-

??篇文章的主要目的是展示在后毛??時代. 中國對韓半??政策是以-충 "平衡??木" 포适??正在變化的國內, 地區和全球形勢. 타不規則地演變了궤개??段, 首先??我??熟悉的-개朝鮮的政策到法理上-개朝鮮??上-개朝鮮-개韓國的政策, 然后又到法理上和??上是-개朝鮮-개韓國的政策. 中國想??加自己的向現代化??的民族力量, 提高國??地位, ??-충現??利益的??略指??了中國的對韓半??政策. 北京保持-충雙??迹的方法; -方面타在1992年8月??韓國建立了外交??系??推??충地綠經??系; 영-方面타又??北韓保持-충??統的地綠??略??系.

Fluconazole로 치유된 안면백선 1예

이병송,윤태진 대한화학요법학회 1996 대한화학요법학회지 Vol.14 No.1

저자들은 fluconozole로 치료한 T. mentagrophytes에 의한 안면백선 1예를 경험하고 이를 문헉고찰과 함께 보고한다. Fluconazole is a triazole antifungal agent with a primarily fungistatic action in vitro. Its spectrum includes dermatophyte, filamentous, dimorphic and dermaticaceous fungi and some yeast species. Fluconazole inhibits the cytochrome P-450 dependent 14-demethylase (lanosterol N-demethylase), therefore inhibiting conversion of lanosterol to ergosterol. At low concentrations there is inhibition of the enzyme 14-demethylase and ergosterol synthesis, with an elevation in the ianosterol/ergostetrol ratio. At higher concentrations there is fungicidal activity with rapid fungal membrance damage. Fluconazole may also inhibits cytochrome C perixidative and oxidative enzymes, causing an increase in intercellular peroxide generation. Furthermore, there may be inhibition of transformation of candidal yeast forms to pseudohyphae. We experienced a patient with timea faciale caused by Trichophyton mentagrophytes. He was treated with fluconazole 150mg weekly for 3 weeks. After completion of therapy, skin lesions showed complete recovery and we could not see any laboratory abnormalities or adverse reactions.