Characterization of the European type of maternal lineage evident in extant Jeju native pigs

Byeong‐Woo Kim,In‐Cheol Cho,Moon‐Sung Park,Tao Zhong,임현태,Sung‐Soo Lee,Hee‐Bok Park,Moon‐Suck Ko,Jun‐Heon Lee,Jin‐Tae Jeon 한국유전학회 2011 Genes & Genomics Vol.33 No.2

Using a partial D‐loop sequence of mtDNA, an intensive analysis was conducted of the maternal lineages of Jeju native pigs (JNPs) from Korea. In total, 100 mtDNA sequences were obtained from Asian wild boars (AWBs), European wild boars (EWBs), Asian domestic pigs (ADPs), European domestic pigs (EDPs), and JNPs and were used for phylogeny and network analyses. Two distinct JNP groups - one (JNPA) in the Asian cluster and the other (JNPE) in the European cluster - were identified in the estimated phylogenetic tree and network. The maternal lineage of JNPE was the closest to that of EWB and a clear haplogroup was identified that shared an identical haplotype (hap04) among 15 individuals of JNPE and 2 individuals of EWB. A Landrace and an EWB shared hap03with a JNPE. EWB, Landrace, Large White, and Duroc formed two clear haplogroups with JNPE in a parsimonious medianjoining network analysis, suggesting that an obvious maternal contribution of EDP has occurred in JNPE in recent years. A pair‐wise mismatch analysis also indicated that JNPE may have experienced a sudden population expansion, suggesting a more recent establishment compared with the gradual population expansion of JNPA. The JNPE group therefore should be further evaluated in order to decide whether this group should be culled or accepted into further programs for maintenance of the JNP population as a pure breed.

소아 야뇨증에 대한 임상적 고찰

김승수,곽병곤,김영대,김우경,김영균,이종국 白中央醫療院 2005 仁濟醫學 Vol.26 No.1

Objectives: The aim of this study was to describe the clinical characteristics of enuretic children and to evaluate the efficacy of treatments. Methods: Our study subjects enrolled were 52 children with nocturnal enuresis, who visited outpatient clinic of pediatrics in Seoul Paik Hospital and Ilsan Paik Hospital, during the period from October 1998 to August 2004. The data of gender, age, family history, symptom, clinical type of enuresis and drug therapy were collected and analyzed retrospectively. Results: The male : female ratio was 2.25 : 1. The mean age at diagnosis was 6.8 years with an age range from 4.3 years to 12.5 years. Fourty five cases(86.5%) were diagnosed as primary enuresis and 7 cases(13.5%) were diagnosed as secondary enuresis. A family history of enuresis was noted in 5 cases(9.6%) including 4 cases with parental enuresis history. The accompanying urinary symptoms were urgency(11.5%), frequency (7.7%), and dysuria(2.0%). Conclusion: We found frequent occurrences of enuresis in boys and primary type and an occasional association with a family history of enuresis. The two main treatment modalities were behavior control with an alarm system and drug therapy with imipramine, desmopressin, or oxybutynin. We recommend continuous drug medications to control nocturnal enuresis along with encouragement.

정신분열병에 대한 리스페리돈의 효과 및 안정성

이민수,김용구,김영훈,연병길,오병훈,윤도준,윤진상,이철,정희연,강병조,김광수,김동언,김명정,김상훈,김희철,나철,노승호,민경준,박기창,박두병,백기청,백인호,손봉기,손진욱,양병환,양창국,우행원,이정호,이종범,이홍식,임기영,전태연,정영조,정영철,정인과,정인원,지익성,채정호,한상익,한선호,한진희,서광윤 大韓神經精神醫學會 1998 신경정신의학 Vol.37 No.1

연구목적 : 본 시험의 목적은 임상시험 시작전에 연구자들을 대상으로 PANSS Workshop을 통하여 PANSS, ESRS에 대한 국내에서의 표준화 작업을 구축하고 새로운 정신병 치료제인 리스페리돈의 효과와 안정성을 재확인하여 리스페리돈 사용에 대한 적정화를 이루는데 있다. 연구방법 : 1996년 4월부터 1996년 9월까지 국내 39개 대학병원 정신과에 입원중인 혹은 증상이 악화되어 입원하는 정신분열병 환자 377명을 대상으로 다시설 개방 연구를 시행하였다. 1주일간의 약물 배설기간을 가진후, 리스페리돈을 8주간 투여하였고, 기준점, 1주, 2주, 4주, 그리고 8주후에 평가되었다. 용량은 제1일에는 리스페리돈 1mg씩 1일 2회, 제2일에는 2mg씩 1일 2회, 제3∼7일에는 3mg씩 1일 2회 투여하였다. 이후 환자의 임상상태에 따라 임의로 증량할 수 있으며, 최대 일일 16mg을 초과하지 않도록 하였다. 추체외로 증상을 조절하기 위한 투약을 허용하였다. 임상증상 및 부작용의 평가는 PANSS(Positive and Negative Syndrome Scale), CGI(Clinical Global Impression) 그리고 ESRS(Extrapyramidal Symptom Rating Scale)을 사용하였다. 연구결과 : 377명중 343명(91%)이 8주간의 연구를 완결하였다. 치료 종결시점인 8주후 PANSS 총점수가 20% 이상 호전된 경우를 약물 반응군으로 정의할때, 약물반응군은 81.3%였다. 리스페리돈에 반응하는 예측인자로는 발병연령, 이전의 입원 횟수, 유병기간이 관련 있었다. 리스페리돈은 1주후부터 PANSS양성, 음성, 및 일반정신병리 점수상에 유의한 호전을 보여 효과가 빨랐다. CGI의 경우도 기준점에 비해 1주후부터 유의한 감소를 나타내었다. ESRS의 경우, 파킨슨 평가점수는 기준점과 비교해 투여 1주, 2주, 4주후 유의하게 증가되었다가 8주후 기준점과 차이가 없었다. Dystonia 평가점수는 1주후만 유의한 증가를 보였으며, dyskinesia 평가점수는 유의한 차이가 없었다. 혈압, 맥박수의 생명징후 및 일반 혈액학 검사, 생화학적 검사, 심전도 검사에서 유의한 변화는 없었다. 결 론 : 이상의 다시설 개방 임상 연구를 통해 리스페리돈은 정신분열병 환자에서 양성증상뿐만 아니라 음성증상 및 전반적인 증상에도 효과적인 것으로 사료된다. 보다 명확한 평가를 위해서는 다른 항정신병약물과의 이중맹검 연구가 필요할 것으로 생각되며, 또한 장기적 치료에 대한 평가도 함께 이루어져야 하겠다. Objective : The purpose of this study was to investigate the efficacy and safety of risperidone in the treatment of Korean schizophrenic patients. Method : This multicenter open study included 377 schizophrenic patients drawn from 39 university hospitals. After a wash-out period of 1 week, the schizophrenic patients were treated with risperidone for 8 weeks and evaluated at 5 points ; at baseline, and 1, 2, 4 and 8 weeks of treatment. The dose was increased from 2mg/day(1mg twice daily) to 6mg/day(3mg twice daily) during the first week and adjusted to a maximum of 16mg/day over the next 7 weeks according to the patient's clinical response. Medication to control extrapyramidal symptoms was permitted. The psychiatric and neurological status of the patients was assessed by PANSS, CGI, and ESRS scales. Results : 343(91%) of 377 patients completed the 8-week trial period. Clinical improvement, as defined by a 20% or more reduction in total PANSS score at end point, was shown by 81.3% of patients. The predictors of response to risperidone were associated older age, shorter duration of illness, fewer previous hospitalization. Risperidone had rapid onset of action ; a significant decrease of the total PANSS and three PANSS factor(positive, negative, general), and CGI was already noticed at the end of first week. For the ESRS, parkinsonism rating scores were significantly increased until week 4 comparing with baseline. Dystonia rating scores were significantly increased until week 1, and dyskinesia rating scores were not significantly changed during the study. Laboratory parameters including vital sign, EKG, hematological, and biochemical values showed no significant changes during the trial. Conclusions : This study suggests that risperidone is generally safe and effective against both the positive and negative symptoms in our group of patients.

ID-GC/MS에 의한 쌀 중의 잔류농약 인증표준물질

김유곤,김달호,백병천,이우범 國立 麗水大學校 환경문제硏究所 2001 環境硏究論文集 Vol.4 No.-

The CRM used in evaluation of effectiveness, calibration or analysis equipment when you analyse in material of rice powder or similar matrix. Analytical items were γ-HCH, p,p'-DDE, endosulfan-2, samples of achieved pertreatment analized each component indivisually. Control mode of MS used selected ion monitering(SIM). GC is 6890GC of Agilent Technology company and MS is JMS 700 of Jeol company. MS operating condition was Electron impact ionization with 70eV. 300A, GC operating condition was Column : Rtx-5MS 30m*0.25m ID*0.25um, Oven Temp 90℃(3min)-20℃/min-280℃(2min), Injection port 250℃, splitless, Carrier Gas He with 1mL/min. Water contents was 12.534% and dry mass correction factor 1.143. CRM certified values of the experiment result were p,p'-DDE 0.165±0.011,mg/kg, γ-HCH 0.0129±0.0011mg/kg, endosulfan-2 0.0216±0.0027mg/kg. The numbers following the symbol ± are the numerical values of expanded uncertainties, U=ku_(c) of measurement values preceding the symbol. The level of confidence for the expanded uncertainties are 96% with k=2.

고주파수 PWM 제어를 이용한 ABS의 특성 평가에 관한 연구

김병우,이용주,박호 한국공작기계학회 2004 한국생산제조학회지 Vol.13 No.2

In general the surge pressure generated in hydraulic systems causes noise, vibration and odd effect to the system. To reduce the surge pressure, high frequency PWM control of 20KHz was attempted. To estimate the braking noise caused by surge, a vehicle equipped with on-board ABS hydraulic modulator has been experimented with respect to the various breaking condition. Though this experiments, it was found that breaking noise has been reduced using high frequency PWM control method compare with low frequency method. To evaluate high frequency control in practice, including verification of general functionality, EM tests was experimented. Its was found that it is necessary to have the solution to electromagnetic interference(EMI) generated by switching elements.

공학도의 의사소통과 리더십, 팀웍을 배양하기 위한 공학 소양교육 방법

김병재,김석우,최재선 明知大學校 産業技術硏究所 2004 産業技術硏究所論文集 Vol.23 No.-

Engineering performance skills such as communication, leadership, and teamwork are essential for undergraduate students. This thesis proposes and integrated curriculum for engineering students to teach communication, leadership, and teamwork skill for one semester(16 weeks). When an engineering college is to develop such an integrated curriculum based on educational objectives, the college should emphasize performance skills. That would bring some synergy effect in education.

SECKEL 증후군 환자의 증례보고

김추성,김재곤,백병주,양연미,정진우 大韓小兒齒科學會 2010 大韓小兒齒科學會誌 Vol.37 No.1

Seckel 증후군은 상염색체 열성 유전질환으로 태내에서의 성장지연에 따른 출생 후의 소인증, 심한 소두증을 나타내며, 큰눈과 돌출된 코, 후퇴된 하악을 동반한 새 모양의 안모가 특징적이다. 다른 임상증상으로는 관절의 결함, 내반족, 드문드문 난 모발, 비뇨생식기의 이형성, 정신지체, 혈액학적 이상 등이 나타날 수 있다. Seckel 증후군의 구강 내 소견으로는 부정교합, 법랑질 저형성증, 일부 치아의 선천적 결손, 우상치 등이 나타날 수 있다. 본 증례는 Seckel 증후군을 보이는 두 증례에서 발육부전, 새 모양의 안모, 비뇨기과적 기형, 치아의 저형성과 부정교합 등 다양한 전신증상과 구강 내 증상들을 보였기에 이를 보고하는 바이다. Seckel syndrome is an autosomal recessive disorder characterized by intrauterine growth retardation and post-natally by dwarfism, severe microcephaly, bird-headed profile with receding chin, prominent nose, joint defects, clubfoot, sparse hair, malformation of genitourinary tract and rectum, mental retardation and hematological disorders. There is also a reduction in the number of blood cells. Dental anomalies of Seckel syndrome are crowded teeth with malocclusion, enamel hypoplasia, absence of some teeth and taurodontism. This report described the oral and maxillofacial manifestations of children associated with Seckel syndrome. Children with Seckel syndrome have several dental and skeletal irregularities. The purpose of this study was to report the dental and medical characteristics of the patient and review the literatures of Seckel syndrome.

사이벡스를 이용한 보강트레이닝 처방 : 근력, 파워, 근 지구력을 중심으로

金秉洙,金義煥,李康雨 龍仁大學校 1992 용인대학교 논문집 Vol.8 No.-

The Purpose of this study was to investigate the prescription of isokinetic strength, muscular power and endurance developed by isokinetic training.Subject was one Korea eleite athlete for male. Isokinetic strength was defined as the maximun peak torque achieved through a range of motion at 240˚/sec and muscular endurance was defind to be the work output perforned by 50 repetitions at 240˚/sec on the lumex Cybex-II. Subject performed in 50 sec.8 repetitions at 60˚/sec., 3 sets 10 repetitions at 240˚/sec.in 3 day week for 8 weeks. The findings of the present case study were as follows. 1) The percent ratio(%) increments of isokinetic strength for right(R.) and left(L.) knee was 9% than pre -test. 2) The percent ratio(%) increments of isokinetic muscular power for knee flexor was 7% than pre -test 3) The percent ratio(%) increments of isokinetic muscular endurance for L.knee extensor, L.knee flexor, R.knee extensor, and R.knee flexors were 11%, 18%, 16% and 22% than pre -test respectively. Within the scope of this study, the finding have suggested the following conclusions ; First, to check injury factors should be useing scientific instrument Cybex-II system. Second, to improve strength, power and muscular endurance should be useing isokinetic training be Cybex - II system.

변위 매복된 상악 중절치의 교정적 맹출 유도

김재곤,정진우,백병주,양연미,이용희 大韓小兒齒科學會 2004 大韓小兒齒科學會誌 Vol.31 No.3

매복치아란 어떤 원인에 의해 적절한 맹출 시기를 넘어서까지 맹출 되지 않고 구강점막하나 악골 내에 묻혀 있는 치아를 말하여, 매복치의 일반적인 발생 빈도는 상, 하악 제 3대구치, 상악 견치, 상, 하악 소구치, 그리고 상악 중절치의 순서로 나타난다. 매복 원인으로는 (1) 폐쇄적인 원인 (과잉치, 치아종, 치배의 이소위치) (2) 외상성 원인 (연조직 치유에 의한 폐쇄, 만곡치, 정지된 치근 발육, 급성 외상성 함입)을 생각할 수 있다. 외과적인 노출 후에 교정 장치를 이용한 치아맹출 방법에 있어 첫째로 치열궁내에서 치아를 위해 적절한 공간이 마련되어야 하며, 외과적 노출에 있어 작은 어태치먼트를 수용하는데 충분한 법랑질 표면을 제공, 오염될 우려 없이 접착 술식이 가능할 수 있도록 지혈에 충분할 만큼 공간을 제공하여야 한다. 어태치먼트를 치아에 부탁, 정출력을 유지하기 위해 결찰 선재와 고무줄(elastic)을 이용하여 외부 장치와 연결한다. 본 증례들은 상악 중절치의 맹출 지연을 주소로 본원에 내원한 환아들로 방사선사진상 상악 중절치의 변위 매복을 발견하였으며, 외과적 노출과 교정력을 통하여 본래 위치로 유도하여 양호한 결과를 얻어 맹출 유도의 방법 제시에 도움이 되고자 보고하는 바이다. Impaction is generally defined as the lack of eruption of a tooth after the normal age for the eruption. An impacted tooth may appear blocked by another tooth, bone, or soft tissues, but cause of tooth impaction is often unknown. The clinician should consider the various treatment options available :(a) No treatment and observation, (b) surgical exposure and orthodontic traction (c) auto transplantation (d) extraction. These cases were about the patients with delayed eruption of maxillary central incisor. We surgically exposed impacted tooth and guided it into normal position by the orthodontic traction. Especially, in case 1. #21 was ectopic impacted state with root dilaceration. It is required to examine further root development and alignment of dentition serially.

효모시스템에서 Human Transglutaminase C(TGase 2)의 발현에 관한 연구 : C-말단부위의 결손효과 An Effect of a Domain from Carboxyl Terminal Deletion of the Enzyme

우상규,정선미,이상기,안병윤,김희철 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.3

Transglutaminase(TGase Ⅱ)는 펩타이드-결합 글루타민 잔기와 펩타이드-결합 라이신잔기 및 1차 아민류를 포함하는 다양한 화합물과의 교차결합에 의하여 단백질의 변형을 촉매하는 효소이다. 동물세포에는 구별가능한 5종류의 TGase가 존재한다[TGase Ⅰ(또는 K), Ⅱ(C), Ⅲ(E), Ⅳ(P)와 혈장단백 ⅩⅢa]. 그들은 아미노산 서열에서 N-말단에 약 70%의 상동성을 갖고 있으며, C-말단의 경우에는 거의 상동성이 부위(N-말단 2/3)와 비상동성 부위(C-말단 1/3 부위)의 역할을 알아보기 위해 전체효소 또는 C-말단이 결손된 형태의 효소를 Saccharmoyces cerevisiae에서 발현 하고자 하였다. Southern blot, 역전사 중합 연쇄 반응, Western blot등을 통해 전체효소 및 C-말단 결손형 효소가 형질전환된 효모에서 발현되는 것을 확인하였다. Succinylated casein에 [1,4-^14C]-putrescine이 통합되는 반응을 이용한 활성 측정에서 C-말단이 결손된 TGase Ⅱ는 같은 세포 농도에서 전체 TGase Ⅱ 효소와 비교하여 유사한 수준의 활성을 나타내었다. 이러한 결과들은 사람 TGase Ⅱ의 경우 N-말단의 높은 상동성부위가 효소의 역가결정에 중요하다는 것을 나타내 준다. 효모시스템에서 발현한 결과 효소의 역가가 나타났으나 높지 않았으며 발현된 양이 극히 낮아서 단백질의 정제나 특성연구를 위하여 발현벡터의 개량을 포함한 방법의 개선이 필요하다고 사료된다. 흥미롭게도 TGase Ⅱ의 발현이 효모의 성장을 저해하는 것으로 나타나 이러한 현상이 표피세포의 각질화에 수반된 세포의 죽음과 유관한지의 가능성에 대한 보다 자세한 연구가 필요하다. In an effort to understand the role of the conserved domain and of the heterologous one-third part of the carboxy terminal domain of transglutaminase C (TGase Ⅱ), attempts were made to express TGase Ⅱ cDNA of human origin in yeast Saccharmoyces cerevisiae as in a full-length form as well as in a form of C-terminal truncation. The 2μ-based expression plasmids which contained the TGase Ⅱ cDNA under the gal inducible promoter were introduced into yeast and the maintenance of the full-length and truncated form of the TGase Ⅱ gene plasmids were confirmed by Southern blot. The expression of e results indicate that the N-terminal homologous domain of human TGase Ⅱ retains a catalvtically active domain. The level of TGase Ⅱ expressed in yeast, however, was far lower than satisfactory and other expression system should be sought further characterization of the enzyme. The negative effect of TGase Ⅱ on the growth of yeast is interesting with respect to the physiological effect of TGase Ⅱ in the cornification f epidermal keratinocytes.