Retinoic acid induces expression of Ig germ line α transcript, an IgA isotype switching indicative, through retinoic acid receptor

Mi-Hee Park,박석래,Mi-Ra Lee,Young-Ha Kim,Pyeung-Hyeun Kim 한국유전학회 2011 Genes & Genomics Vol.33 No.1

Retinoic acid (RA) is considered to possess an activity of IgA isotype switching. Thus far, TGF-β1 is known to be the most powerful IgA isotype switch factor. To elucidate the molecular mechanisms underlying the Ig germ line (GL) α transcriptional regulation by RA, we constructed three different sizes of mouse GLα promoter reporters; short-GLα(-130/+14), middle-GLα(-448/+72) and long-GLα(-3028/+72). Based on luciferase assay, RA increased the activity of all three GLα promoter reporters by approximately 2-fold and the effect was further enhanced by TGF-β1. Overexpression of Smad3/4 increased TGF-β1-induced GLα promoter activities but had no effect on RA-induced GLα promoter activities. In order to analyze the characteristics of the RA-inducible GLα promoter region,we also constructed two mutant reporters: Smad3 binding elements (SBEs)-substituted short-GLα (short-GLα mSBE)and Runx3 binding elements (RBEs)-substituted short-GLα(short-GLα mRBE) promoter reporters. Promoter activities of the two mutant reporters to RA were comparable to that of wild type reporter, while those of the two mutant reporters to TGF-β1 were markedly diminished as compared to that of WT short-GLα. Finally, RA-induced GLα transcription was virtually disappeared by LE540, an antagonist of RA receptor (RAR). Taken together, these results suggest that RA induces GLα transcription mainly through RAR pathway, where neither Smad3/4 nor Runx3 is involved.

류마티스관절염 환자의 인슐린 저항성 결정인자

이정욱 ( Joung Wook Lee ),박영은 ( Young Eun Park ),조미라 ( Mi Ra Cho ),백승훈 ( Seung Hoon Baek ),김근태 ( Geun Tae Kim ),이준희 ( Jun Hee Lee ),김성일 ( Sung Il Kim ) 대한류마티스학회 2009 대한류마티스학회지 Vol.16 No.2

Objective: Rheumatoid arthritis (RA) is associated with an increased cardiovascular events. These may be related to insulin resistance (IR). We evaluated status of IR and analyzed the relationship between IR and clinical and laboratory characteristics in patients with RA. Methods: We examined 52 RA patients (43 females) and 52 age and sex matched healthy controls. We measured Homeostasis model assessment (HOMA) IR, calculated according to fasting serum glucose and insulin. Results: In patients, age was 50.8±10.2 years old, disease duration was 42.1±30.9 months. In controls, HOMA IR was 0.62±0.30 and in patients, it was 1.28±0.50. This difference was highly significant (p<0.001). Patients with early RA (disease duration is less than 36 months) were 28, and established RA (more than 36 months) were 24. HOMA IR was significantly higher in patients with established RA (1.42±0.45) than those with early RA (1.16±0.45) (p=0.03), and significantly correlated with disease duration (r=0.36, p=0.01), BMI (r=0.36, p<0.001), total cumulative prednisolon dose (r=0.34, p=0.01). Disease duration and BMI were independent predictors for HOMA IR (p<0.01, p=0.03). Conclusion: In patients with RA, IR measured by HOMA IR was more significantly increased than that of healthy control and significantly correlated with disease duration, BMI, and total cumulative prednisolon dose; however, the determinants of HOMA IR in RA patients were disease duration and BMI.

Purge & Trap-GC를 이용한 의약품 필름코팅 정제 중 잔류용제에 관한 연구

장준식,이명자,소유섭,문춘선,이주헌,박희라,김진숙,강경모,이선옥,방성연,유미자,유문균,금오성,이병욱 식품의약품안전청 2000 식품의약품안전청 연보 Vol.4 No.-

의약품은 약물을 생체에 적풋하기 위하여 유효성분의 효과가 언제나 일정하게 확보되고 사응에 편리하도록 만들어지는 것이므로 유효썽분 이외에 약효에 영향을 주지 않는 성분이 첨가되는 경운가 많다. 이 때 사용되는 용매들은 제피의 광택 및 건쪼시간의 단축 등을 위하여 휘발점이 낮을 용매들이 주로 사용되어진다. 본 연구는 의약품 필름코팅정제 중 잔류용매 4종(chlorofonr benzen, trichloro ethylen, 1,4-dioxane)에 대한 변형된 pirge & trap-GC 장치를 이용한 동시분석방법을 개발하였으며, 각 표준품의 RSD 값은 chloroform 3.03%, benzen 3.17%, trichloroethylen 3.69% and 1,4-dioxane 3.41%였다. 또한 시중 유통중인 의약품 50종에 대하여 잔류웅매 양을 측정하였으며, 검출되는 잔류용매는 한 건도 없었다. This study nras carried out to develope the analytical method for the mixture of chlorefonn, benzen, trichloroethylen and 1,4-dioxane simultaneously and determine the remainingorgauic solvents in coating tablets by Purge & Trap-GC. The results were as follouFs ; 1. Chloroform, benzen, trio:tloroethylen and 1,4-dioxane separated by tenax #5 trap by HP-624GC column by terrlperature programming. The peaks were separated completely at retentiontime of 6.88min for chloroform, 8.21min for benzen, 10.38miu for trichloroethylen and 11.95minfor 1,4-dioxane. 2. Standard RSD were individually chloroform 3.03%, benzen 3.17%, trichloroethylen 3.69%and 1,4-diorane 3.41%. 3. 60 samples were not detrcted chloroform, benzen, trichloroethylen and 1,4-dioxane.

중년여성의 운동 예측모형

이미라,소희영 충남대학교 간호과학연구소 2002 충남대 간호학술지 Vol.5 No.1

This study was conducted to test the hypothetical model designed to explain exercise behavior of middle-aged women. This hypothetical model was based on behavior-related theories and exercise-related literature. Exogenous variables were exercise habit and perceived subjective norm. Endogenous variables were exercise benefit, exercise barrier, exercise-related affect, exercise self-efficacy, exercise intention, and exercise. A convenience sample of 152 middle-aged women was studied. SAS PC Program and LISREL 8.12a program were used for descriptive statistics and linear structural relationship(LISREL) modeling. The results were as follows. 1)The overall fit of the hypothetical model was acceptable(χ2 34.86(p=0.0015), χ2/df 2.49, RMSEA 0.09, standardized RMR 0.05, GFI 0.95, AGFI 0.85, NFI 0.92, NNFI 0.87, CFI 0.95). 2)The results of statistical testing of hypotheses were as follows. (1)The varibles in this hypothetical model explained 95% of the variance of the exercise behavior. (2)Habit was the most important variable to explain exercise behavior, followed by self-efficacy, barrier, affect and benefit. Subjective norm and intention were not significant predictors of exercise behavior. Habit and self-efficacy had a significant direct effect on exercise behavior. Barrier and intention did not directly influence on exercise behavior. Habit, benefit, barrier and affect had a significant indirect effect on exercise behavior through other variables in this hypothetical model. Norm had no indirect effect on exercise behavior. (3)The direct path between habit and benefit, habit and barrier, habit and self-efficacy, norm and barrier, benefit and affect, barrier and self-efficacy, and affect and self-efficacy were significant. The indirect path between habit and affect, habit and self-efficacy, benefit and self-efficacy, benefit and intention were significant. In conclusion, this study verified that the hypothetical model constructed in this study could be used effectively as a reference for further structural modeling or interventional studies.

폐 내 병변으로 인한 소아 급성 호흡부전의 원인 질환과 예후

이혜영,박지숙,최희라,서지현,김점수,임재영,최명범,박찬후,우향옥,윤희상 대한소아과학회 2004 小兒科 Vol.47 No.5

컴퓨터를 이용한 혈액학 교육시스템의 개발

이기형,손보라,윤희석,조원덕,김승택 충북대학교 의학연구소 2001 忠北醫大學術誌 Vol.11 No.1

연구목적 : 전통적인 의학교육 시스템은 최근 개발되고 있는 여러 가지 영상 진단법 등 시각적 자료를 전달하는데 한계가 있으며, 이러한 한계는 모든 분야에 걸쳐서 나타나지만 특히 혈액학이나 심장학 등 시각적인 자료가 진단에 필수적인 분양에서 두드러진다고 할 수 있다.본 연구는 혈액학 교육에 사용되던 기존의 강의실 교육에 시각적 자료가 첨부된 CD-ROM을 개발하여 혈액학 교육 시스템을 개선하기 위한 것이다. 대상 및 방법 : 기존의 혈액학 교육에 있어서 필수적으로 강의 되어오던 자료를 수집하여 체계적으로 정리하였다.강의 자료에 따라서 필요한 영상 자료를 충북대학교 병원에 입원한 혈액질환 환자들의 검사 자료로부터 수집하여 컴퓨터를 기반으로 한 의학영상 처리 및 분석시스템을 통하여 이미지 파일로 변형하였다.또한 입원 환자들 중 대표적인 증례들을 수집하여 실제 임상 진료와 유사한 형태의 증례를 통한 학습을 할 수 있도록 하였다. 결과: 본 CD-ROM은 혈액학 교육에 필요한 대부분의 영역을 포함한다 각 질환의 병태생리, 진단, 임상양상, 치료 및 예후 등 전통적으로 강의되어 오던 강의록에 이미지 파일로 변형된 영상 자료를 필요한 곳마다 링크시켰다.따라서 학습자는 특별한 장치 없이 텍스트 및 영상을 한 화면에서 볼 수 있어 전통적인 교육 시스템에 비하여 시,공간적인 장점을 갖는다 할 수 있다.또한 입원 환자들로부터 수집된 증례를 통하여 실제 임상 진료와 매우 유사한 경험을 하도록하여 교육효과를 증대시켰다. 결론: 혈액학 교육에 있어서 멀티미디어적 효과를 극대화시켜 질환의 병태생리 등 강의실 교육자료뿐 아니라 진단에 필수적인 시각적 자료를 손쉽게 접할 수 있는 CD-ROM의 개발로 전통적인 강의방법이 갖는 한계를 개선시킬 수 있을 것으로 기대된다. Purpose : The traditional medical education system has its limitations in effectiveness of conveying informations in multiple modalities used in diagnosis of patients with hematologic diseases.This study was performed to develop such a multimedia system using computer based CD-ROM, which includes visual assistant systems, several hematological case studies as well as the traditional text-wise education systems. Materials and Methods: The informations about hematologic diseases were compiled and organized.The main visual data was peripheral blood smears, bone marrow data, and several laboratory results accrued from patients admitted to Chungbuk National University Hospital.Each visual data were transformed through computer-based medical image processing and analyzing system and saved as to JPEG files.Case studies were also acquired from admitted patients and the laboratory data of that patients were used unmodified as possible as. Results : The CD-ROM covers almost all kinds of hematologic diseases that must be included in ordinary medical education courses.The text part includes pathophysiology, diagnosis, clinical features, treatment and prognosis of each hematologic diseases.The essential visual data were linked to each text part and accessible with ease, by one click.Case studies were simulated to actual clinical settings and presented step by step from diagnoses to treatments. Conclusion: A multimedia approach for the study of hematologic diseases has been developed and available in CO-ROM format.This system has advantage over traditional education system in conveying all kinds of visual data which is essential in educating hematologic disorders.

전국 10개 종합병원 중환자실 환자의 직장내 Vancomycin-resistant Enterococci(VRE) 보균율, 보균의 위험요인 및 유전적 다양성 조사

정희진,송준영,엄중식,김우주,최선주,최정현,이혁,정문현,이광미,신동현,손보라,최종성,우흥정,권정아,이경원,최강원,박승철 대한감염학회 2002 감염 Vol.34 No.5

첨가성분에 따른 부가중합 실리콘 인상재의 세포독성에 관한 연구

조리라,고성희,정경호,김경남 대한치과기재학회 2001 대한치과재료학회지 Vol.28 No.3

The purpose of this study was to evaluate the biocompatibility of polyvinyl siloxane impression materials and it's components using cell culture techniques. In the cytotoxicity test, using the culture of L929 mouse fibroblast, the degree of gross cytotoxic effects on the cells by changes in the cell morphology was scored for each materials. And the changes in the cell membrane permeability was tested using the agar overlay test. The results were as follows: 1.The effect of Examix impression material had on cell viability by evaluating survival rates was more severe than any other materials and newly added surfactants. 2.NP-4, nonylphenoxy polyethylene ethanol homologs, showed some cytotoxicity than PC-550 and BYK066. 3.In the test of the changes in cell membrane permeability using the agar overlay method, any cytotoxicity was recorded in silicone base groups. 3.In agar overlay test, Examix and NP-4 showed most response index and lysis index. 4.The application of surfactant such as PC-550, NP-4, and BYK066 might be considered relatively biocompatible.

Perilla frutescens var. japonica and rosmarinic acid improve amyloid-β<SUB>25-35</SUB> induced impairment of cognition and memory function

Ah Young Lee,Bo Ra Hwang,Myoung Hee Lee,Sanghyun Lee,Eun Ju Cho 대한지역사회영양학회 2016 Nutrition Research and Practice Vol.10 No.3

BACKGROUND/OBJECTIVES: The accumulation of amyloid-β (Aβ) in the brain is a hallmark of Alzheimer’s disease (AD) and plays a key role in cognitive dysfunction. Perilla frutescens var. japonica extract (PFE) and its major compound, rosmarinic acid (RA), have shown antioxidant and anti-inflammatory activities. We investigated whether administration of PFE and RA contributes to cognitive improvement in an Aβ25-35-injected mouse model. MATERIALS/METHODS: Male ICR mice were intracerebroventricularly injected with aggregated Aβ25-35 to induce AD. Aβ25-35-injected mice were fed PFE (50 mg/kg/day) or RA (0.25 mg/kg/day) for 14 days and examined for learning and memory ability through the T-maze, object recognition, and Morris water maze test. RESULTS: Our present study demonstrated that PFE and RA administration significantly enhanced cognition function and object discrimination, which were impaired by Aβ25-35, in the T-maze and object recognition tests, respectively. In addition, oral administration of PFE and RA decreased the time to reach the platform and increased the number of crossings over the removed platform when compared with the Aβ25-35-induced control group in the Morris water maze test. Furthermore, PFE and RA significantly decreased the levels of nitric oxide (NO) and malondialdehyde (MDA) in the brain, kidney, and liver. In particular, PFE markedly attenuated oxidative stress by inhibiting production of NO and MDA in the Aβ25-35-injected mouse brain. CONCLUSIONS: These results suggest that PFE and its active compound RA have beneficial effects on cognitive improvement and may help prevent AD induced by Aβ.

MLVA Genotyping of Brucella abortus isolated from cattle in Korea

Jin-Ju Lee(Jin-Ju Lee),Eun-Ji Yum(Eun-Ji Yum),Eun-Ji Park(Eun-Ji Park),Min-Kyeong Kim(Min-Kyeong Kim),Min-Hoe Lee(Min-Hoe Lee),So-Ra Sung(So-Ra Sung),Hee-Soo Lee(Hee-Soo Lee),Bang-Hun Hyun(Bang-Hun Hy 한국예방수의학회 2019 한국예방수의학회 학술대회자료집 Vol.2018 No.-