Histone deacetylase 3 is selectively involved in L3MBTL2-mediated transcriptional repression

Yoo, Jung-Yoon,Choi, Kyung-Chul,Kang, HeeBum,Kim, Young Jun,Lee, Jeongmin,Jun, Woo Jin,Kim, Mi-Jeong,Lee, Yoo-Hyun,Lee, Ok-Hee,Yoon, Ho-Geun Elsevier 2010 FEBS letters Vol.584 No.11

<P><B>Abstract</B></P><P>This is the first report that L(3)mbt-like 2 (L3MBTL2) specifically interacts with the histone deacetylase domain of histone deacetylase 3 (HDAC3) via its MBT domain. Here, we show that L3MBTL2 selectively interacts with HDAC3, but not other class I HDACs. An in vitro peptide-binding assay demonstrated the specific association of HDAC3 with methylated histone-K20 tail and L3MBTL2. Furthermore, depletion of HDAC3 resulted in a decrease of methylated K20-H4, as well as an increase in acetylated histone H3. Consequently, HDAC3 knock-down selectively suppressed L3MBTL2-mediated transcriptional repression. Taken together, our results reveal the concerted action of both HDAC3 and L3MBTL2 in histone deacetylation and methylation-dependent transcriptional repression.</P><P><B>Structured summary</B></P><P>MINT-7719975: <I>L3MBTL2</I> (uniprotkb:Q969R5) and <I>HDAC3</I> (uniprotkb:O15379) <I>colocalize</I> (MI:0403) by <I>fluorescence microscopy</I> (MI:0416)</P><P>MINT-7719941, MINT-7719921: <I>L3MBTL2</I> (uniprotkb:Q969R5) <I>binds</I> (MI:0407) to <I>HDAC3</I> (uniprotkb:O15379) by <I>pull down</I> (MI:0096)</P><P>MINT-7719991: <I>HDAC3</I> (uniprotkb:O15379) <I>physically interacts</I> (MI:0915) with <I>L3MBTL2</I> (uniprotkb:Q969R5) by <I>anti bait coimmunoprecipitation</I> (MI:0006)</P><P>MINT-7719958: <I>L3MBTL2</I> (uniprotkb:Q969R5) <I>physically interacts</I> (MI:0915) with <I>HDAC3</I> (uniprotkb:O15379) by <I>anti tag coimmunoprecipitation</I> (MI:0007)</P><P>MINT-7719897: <I>HDAC3</I> (uniprotkb:O15379) <I>physically interacts</I> (MI:0915) with <I>L3MBTL2</I> (uniprotkb:Q969R5) by <I>two hybrid</I> (MI:0018)</P>

심근 허혈-재관류 백서 모델에서 mitogen-activated protein kinase 활성화에 대한 α-lipoic acid의 효과

김연경,박은미,최준호,윤경호,유남진,이은미,김남호,정진원,김민선,박병림,오석규 圓光大學校 醫科學硏究所 2006 圓光醫科學 Vol.21 No.1

저체중 또는 과체중 청소년에서 캠프 프로그램을 이용한 영양 교육효과에 관한 연구

성미경,승정자,류화춘,박재년,박동연,최미경,조경옥,최선혜,이윤신,김유경,이은주 대한지역사회영양학회 2003 대한지역사회영양학회지 Vol.8 No.4

This study was performed to evaluate the effectiveness of a nutrition education program developed for nutritionally imbalanced adolescents. A summer nutrition camp was held for 23 overweight and 16 underweight subjects. Its effectiveness with regard to was evaluated at the end of the camp and 6 months later. Nutrition knowledge, nutritional attitude, food behavior, nutrient intake, exercise habit and ideal body figures desired by the subjects. The results showed significantly higher nutrition knowledge scores at the end of the camp as compared to those obtained prior to the camp program, and these scores were maintained for at least six months. Nutrition attitude scores also improved after the education program, and these improved scores also lasted for 6 months. However, the food behavior scores measured 6 months after the education program were not significantly different from those obtained prior to the camp. Also, the exercise habit, the ideal body figures and the body figures desired by the subjects remained unchanged. When nutrient intakes of subjects were assessed before the program and 6 months later, the mean daily vitamin C intake was significantly increased after the education program. Also, the intake of iron from plant food sources increased in the overweight subjects, while less iron from animal source were consumed by the underweight subjects. Both groups tended to consume more vegetables and fruits 6 months after the education program which may have contribute to the higher vitamin C and plant-based iron intakes. These results indicate that a 4-day nutrition education camp program sustained changes in nutrition knowledge and nutrition attitude for 6 months. The increased intake of vegetables and fruits was also achieved through this education program. However, changes in dietary behavior in adolescents may require repeated education.

일부 성인의 치과 공포감 관련 요인

김수경 ( Soo Kyung Kim ),김미희 ( Mi Hee Kim ),최현지 ( Hyun Ji Choi ),황정근 ( Jung Geun Hwang ) 한국치위생학회(구 한국치위생교육학회) 2014 한국치위생학회지 Vol.14 No.6

Objectives: The purpose of the study was to investigate the related factors to dental fear in some adults. Methods: A self-reported questionnaire was filled out by 320 adults in Seoul and Gyeonggi-do from April to June, 2013. Except 14 incomplete answers, data were analyzed by SPSS 18.0 program. The questionnaire consisted of general characteristics of the subjects(7 questions), dental fear related factor(18 questions), distrust for dentist(15 questions), and distrust for dental hygienist(11 questions). The instrument for dental treatment fear was Dental fear Survey(DFS) adapted from Kleinknecht and partly modified by Choi. Out of 20 questions, 18 questions were reconstructed and score by Likert 5 scale. Higher score means higher dental fear. Cronbach alpha was 0.959 in the study. The distrust for dentist and dental hygienist was adapted from Choi and reconstructed and score by Likert 5 scale. Higher score means higher distrust for dentist and dental hygienist. Cronbach alpha was 0.937 in distrust for dentist and 0.874 in distrust for dental hygienist in the study. Results: Those who experienced dental pain tended to have dental fear. The reasons for dental fear were as follows; bad breath(2.96 out of 5 points, hereafter represented as of 2.96/5), dental caries(2.88/5), missing teeth(2.87/5) and tooth pain(2.77/5). The distrust for the dentist was the main dental fear in the adults. Conclusions: The dental fear was closely related to gender, experience of dental pain, oral symptoms and distrust for the dentist. As the psychological pain in the patients was mainly influenced by the trustful atmosphere, it is necessary to make the patient easy before treatment.

목표달성에 따른 보상이 학습공동체 참여 대학생들의 학업적 자기효능감에 미치는 효과

최경미 김천대학교 2019 논문집 Vol.40 No.-

The study recruited learning communities consisting of three to six students to explore the impact of compensation on the academic self-efficacy of university students participating in the study community. The participants were asked to decide the goals of their learning communities and the target achievement plans in advance. Their academic self-efficiency of the participating students was investigated by conducting the same survey before and after the operation of the learning community. In addition, to find out students' learning community processes and goals of the study community, the report was guided by parking specific reports, interim reports, final reports, reflective reports, and comment letters. The MLST-II learning strategy diagnosis test was conducted to identify the learning propensity and type of students participating in the study community. The results of the diagnosis showed a negative tendency toward learning compared to students in the Seoul metropolitan area due to low expectations and low internal motivation, and about 60 percent of their study type was very low, potential and stagnant. Nevertheless, the academic self-efficacy of students participating in the study community increased 0.17 points, and even those with negative learning tendencies achieved their goals by 30% through the learning performance.

한의진단명과 진단요건의 표준화 연구 III : 3차년도 연구결과 보고

최선미,양기상,최승훈,박경모,박종현,심범상,김성우,노석선,이인선,정진홍,이진용,김달래,임형호,김윤범,박성식,송태원,김종우,이승기,최윤정,신순식 한국한의학연구원 1997 한국한의학연구원논문집 Vol.3 No.1

The diagnostic requirements were suggested and explained regarding the systems of differentiation of symptoms and signs in the third year study of standardization and unification of the terms and conditions used for diagnosis in oriental medicine. The systems were as follows : - analyzing and differentiating of epidemic febrile disease - analyzing and differentiating in accordance with the Sasang constitution medicine based on four-type recognition - differentiation of disease according to pathological changes of Chong and Ren channels - standards for diagnosis of women's disease - standards for diagnosis of children's disease - standards for diagnosis of motor and sensor disturbance (-muscle. born, joint, etc.) - standards for diagnosis of neuropsychiatric disease - standards for diagnosis of five sense organ disease - standards for diagnosis of external disease The indivisual diagnosis pattern was arranged by the diagnostic requirements in the following order : another name, notion of diagnosis pattern, index of differentiation of symptoms and signs, the main point of diagnosis, analysis of diagnosis pattern, discrimination of diagnosis pattern, prognosis, a way of curing a disease, prescription, herbs in common use, disease appearing the diagnosis pattern, documents. The standards for diagnosis of each disease was arranged by the diagnostic requirements in the following order : another name, notion of disease, the main point of diagnosis, analyzing and differentiating of disease, analysis of disease, discrimination of disease, prognosis, a way of curing and prescription of disease, disease in western medicine appearing the disease in oriental medicine, documents.

대학생의 봉사활동 의식 수준별 성격 특성 연구

최진승,한미옥,김경원 동아대학교 교육대학원 2002 동아교육논총 Vol.28 No.-

This study research on service cognitions of college's students and their personality traits. The subjects are 1,417 college students (504 male students and 913 female students). The used tools are college student's personality test and questionnaires of college student's service activities. The sub-factors of personality traits are 12 traits as responsibility, leadership, diligent, independent, planning, tolerance, self-esteem, legality, generosity, anxiety, impulse, and masculinity. The categories of service activities on college students are 11 items as the importance of service activity to college students, the desire of service activity to college students, the beginning times of service activity to college students, the expense of service activity of college students, the times in one year of service activity to college students, the satisfactions of service activity to college students, the obstacles of service activity to college students, the obstacles of service activity to college students, the advantages of service activity to college students, the credit permissions of service activity to college students, the areas of service activity to collee students, the perspectives in future time of service activity to college students. From the results and discussions, conclusions as follows: 1.The college students on higher level traits as level traits as responsibility, leadership, diligent, planning, tolerance, self-esteem, legality, generosity, impulse, and masculinity recognize the importance of service activity. 2.The college students on higher level traits as responsibility, leadership, diligent, planning, tolerance, self-esteem, legality, and generosity recognize the importance of service activity. But the college students on higher level traits as independent recognize the unimportance of service activity. 3.The college students on higher level traits as leadership tolerance, self-esteem, and legality rrecognize the desire of service activity. 4.The college students on higher level traits as tolerance, legality, and generosity recognize the importance of self-expenses. 5.The college students on higher level traits as planning, tolerance, self-esteem, legality, generosity, and masculinity recognize the more than five times in a year as college students' service activity. 6.The college students on higher level traits as responsibility, leadership, diligent, independents planning, tolerance, self-esteem, legality, generosity, impulse, and masculinity recognize the satisfaction of service activity. 7.The college students on higher level traits as responsibility, leadership, planning, tolerance, and self-esteem recognize the obstacle as misunderstanding of college students, but the college students on higher level traits as independents recognize the obstacle as lake of systems and support from college and govemment. 8.There are no differences among the college student's traits and college student's advantages as spirits of sacrifice, professional knowledge, and a lot of times in college courses. 9.The college students on higher level traits as leadership, diligent, planning, tolerance, self-esteem, generosity, impulse, and masculinity recognize the importance of the credit permissions of service activity to college students. 10.The college students on higher level traits as responsibility and masculinity recognize the importance of visiting home for orphans, the college students on higher level traits as leadership recognize the importance of helping farm country, and the college students on higher level traits as independence recognize the importance of helping homelessness parents. 11.The college students on higher level traits as leadership, planning, tolerance, self-esteem, legality, and generosity recognize the positive perspectives in future time of service activity to college students.

병원영양사의 특성과 영양관리업무 수행과의 관련성에 관한 연구 : 서울시내 종합병원을 중심으로 in Seoul

최미영,장유경 漢陽大學校 韓國生活科學硏究所 1988 韓國 生活 科學 硏究 Vol.- No.6

The complexities of modern medicine and the demand for high quality nutrition care services challenge the adequacy of traditional function and roles of the clinical dietitian. This study was designed to examine the relationship between nutritional care services and characteristics of hospital dietitians in Seoul. Data were collected from 51 dietitians of 51 hospitals that occupy 62.2% of the total number of such hospitals in Seoul during the month of September, 1986. Informations were obtained by questionnaires and interviews. The findings are: (1) Current status and position of the hospital dietitians have been progressed in a little. The following go up according to the increase in bed capacity in the hospital: the working career of dietitians at the present position, the amount of salary, the number of dietitians, the frequency of food service by one dietitian per diem and the independence of the department of nutrition. The nutritional care services performed as follows: generally nutritional care services is unregular, nutritional education and counseling activities aren't performed. (2) The characteristics of dietians such as the bed capacity of the hospital, the number of dietitians, the number of food services per diem, independence of nutrition dept., year working at the present position, the exitence of independent nutrition counseling room were significantly correlated with nutritional care services. (3) Nutritional education and counseling activities showed relatively high correlation. Viewed in this light, the improvement of the status of dietitian and high quality nutritional care services that is more strongly supported by the appropriate laws and institutions, college of education curriculum and continuing education.

주거지역에 따른 도시노인의 생활만족도 연구

최미경,박혜인 啓明大學校 生活科學硏究所 1991 科學論集 Vol.17 No.-

The purpose of this study is find out methods to promote welfare of the elderly by analyzing their family environmental variables and by investigating the life satisfaction. The aged over 60 years old living in Daegu were selected out the subject of investigation and cluster sampling was carried out intentionally by categorizing their residence area. The results of this research can be summarized as follows: According to the results analyzed the life satisfaction of the aged with demographic variables it contained meaningful differences in age, but not in gender and religion. The school career could not effect immediately on the life satisfaction of the aged. And yet it could be concluded that school career could have effects on it indirectly because other various environmental variables became better in accordance with high academic background. The extent of life satisfaction of the aged was found out significant differences according to economic level, objective class, and occupation for the elderly. Both objective class and subjective economic level had influence on the degree of life satisfaction of the aged and the female aged of low income classes was the lowest group. Regarding the degree of life satisfaction related to the family pattern of the aged, the aged of low income classes made meaningful differences especially in that of life satisfaction to the type of living together their offspring, and that of life satisfaction of the aged living together their children was higher than that of the aged living without their children. Participation of family role displayed meaningful differences in sex, age, school career, job, family pattern, class and subjective economic level. As mentioned above, the results analyzed environmental variables effecting on the degree of life satisfaction of the aged indicated meaningful differences statistically according to demographic variables, economic conditions and family environment.

서울시내 직장인의 식습관과 건강에 관한 연구

최미경,김정미,김종군 한국식생활문화학회 2003 韓國食生活文化學會誌 Vol.18 No.1

본 연구는 서울시내 직장인의 식생활태도 및 식습관과 건강과의 관계를 알아보기 위하여 서울에서 직장을 다니고 있는 직장인 389명(암자 224명, 여자 165명)을 대상으로 설문조사를 하였으며, 그 결과는 다음과 같다. 1.조사대상자의 연령은 30~40세가 50.9%, 직업은 사무직·교사·공무원이 46.8%, 교육수준은 대졸이 51.2%, 가족형태는 배우자 및 자녀와 함께 사는 직장인이 29.3%로 가장 높게 나타났다. 2.조사대상자의 건강과 관련된 사항 중 현재 건강상태는 보통이다가 69.4%이며, 평소 건강에 대한 관심은 많다가 55.0%로 높은 관심을 보이고 있다. 평소 운동의 규칙성을 보면 운동을 하지 않는다가 48.8%로 높게 나타났다. 수면상태는 보통이다가 46.8%, 충분하지 못하다는 43.9%로 나타났으며, 흡연양은 담배를 피우지 않는다가 41.6%, 음주횟수로는 주 1~2회가 74.8%로 높게 나타났다. 3.식생활태도에 관한 특성 중 하루 식사 횟수는 2회가 61.4%로 가장 높았으며, 식사시간은 규칙적이다가 53.2%, 규칙적이지 않다는 46.8%로 비슷하게 나타났으며, 식사시 소요시간은 10~30분 미만이 69.2%로 가장 많았으며, 식사량은 거의 일정하다가 62.0%, 일주일에 아침식사를 하는 횟수에 대한 결과는 3~4회가 28.0%로 나타났고, 결식은 아침에 한다는 응답자가 71.4%로 나타났고, 기 이유는 시간이 부족해서 (46.5%), 습관적으로 (22.6%) 순이었다. 편식을 하지 않는 직장인들이 56.5%이며, 하루 동안 간식횟수는 1~2회가 72.2%로 대부분의 직장인들은 간식을 하는 것으로 나타났다. 식욕상태는 보통이다가 43.2%, 좋은 경우는 37.3%로 대체로 식욕이 좋은 것으로 나타났으며, 평소 선호하는 음식맛은 매운맛이 40.3%로 가장 높게 나타났다. 건강보조식품섭취에 대한 결과는 가끔씩 섭취한다가 51.7%, 규칙적으로 섭취한다는 14.4%로 나타났으며, 그 이유는 피로회복을 위해서 (51.7%)가 가장 많았으며, 다음으로는 질병의 예방 및 치료를 위해서 (21.3%)로 나타났다. 직장인의 식습관을 조사한 결과는 평균 4.43점으로 Fair 집단이고, Fair 집단은 42.4%, Pool 집단은 39.6%로 직장인들의 식습관이 좋지 않은 것으로 나타났다. 4.식생활 태도와 인구 통계적 특성과의 관계는 성별에 따라 유의적인 차이가 있었으며, 운동을 주 1~2회 하고, 수면을 충분히 취하는 경우와 건강상태가 좋은 경우에는 식습관 점수가 높은 것으로 나타났다. 또한 식생활태도에 있어서 연령층이 높을수록 좋게 나타났으며, 직업과의 관계에서는 모든 집단이 식욕상태가 양호하며, 피로회복을 위해 건강보조식품을 섭취하는 경우가 많았다. 이상의 결과를 종합해 볼 때, 직장인들은 건강에 대한 관심도가 높은 반면에 올바르지 못한 건강관리와 식생활태도로 인해 좋지 않은 식습관을 가지고 잇는 것을 알 수 있었다. 잘못된 식습관은 신체적인 건강상태뿐만 아니라 개인의 정서발달에도 좋지 않은 영향을 미치게 되므로, 현대의 직장인들은 식생활 전반에 있어서 바람직한 태도와 규칙적인 운동과 적당한 수면 및 휴식을 취함으로써 건강을 유지하기 위한 올바른 식습관을 실천하도록 노력하여야 할 것이다. To investigate the relationship between dietary attitude and healthy status of office workers in Seoul was to be researched for 389 subjects (male: 224 and female: 165). As a result showed 69.4% of them moderately healthy state and 55.0% had a great interest in health. About 41.6% of the respondents was nonsmoker and 74.8% of them drunk alcohol once or twice a week. Only 28.0% of them responded to have breakfast three to four times a week. About 71.0% responded to skip breakfast', The main reasons for it were 'the lack of time'(46.5%) and 'habitually'(22.6%). The usually preferred taste of food was 66.0%. The reason for it was shown to be 'for relaxation'(51.7%), followed by 'for prevention and treatment of diseases (23.1%). On dietary habit showed the groups of married and professional people won the high scores on it. And the groups of those doing exercise once or twice a week, those having a sufficient sleep and those kept in a good food habit showed the high scores on it. The older age group showed the good dietary attitude regardless of occupation.