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      • KCI등재

        폴리우레탄 인공혈관을 위한 extracellular matrix 기질상의 내피세포이식

        이윤신,박동국,민병구,Lee, Yoon-Shin,Park, Dong-Kook,Min, Byoung-Goo 대한의용생체공학회 1991 의공학회지 Vol.12 No.3

        Many experiments about endothelial cell seeding on artificial vessels were studied and conducted For this one or a combination of the extramatrix was used for the underlying matrix. But we used the whole ECM(extracellular matrix) that made excreated from flbroblasl. In thls study, we obtained human adult omental microvascular endothelium by collagenase digestion and used polyurthane sheets in order to make a new artificial vessel material. We cultured fibroblast on the polyurethane and gelatin - coated polyurethane. After confluent ingrowth we treated the polyure thane with triton in order to destroy the cytoskeleton and nucleus. We observed the preformed extra cellular matrix on the ployurethane and cultured the isolated microvascular endothelium. We also ok served the growth of microvascular endothelium on the polyurethane and gelatin. We conclude that the use of the whole ECM is promising fair as a new underying substrate for endothelial cell seeding on artificial vessels.

      • 어린이 안전 가방 및 부모용 애플리케이션 개발

        이윤신,이화민 순천향대학교 부설 산업기술연구소 2019 순천향 산업기술연구소논문집 Vol.25 No.1

        The number of reported missing children was 27,295 in 2012, 23,089 in 2013, 21,291 in 2014, 19,428 in 2015, and 19,870 in 2016. From 2012 to 2015, less than 10 missing children were reported, but 182 children were not found in 2016. Also, many incidents such as the Incheon Elementary School murder case and Gwangju Jjimtong Bus accident were found to be dangerous for kindergarteners in various crimes. For this reason, We design and develop safety bags and smartphone application for kids. It will help the safety of young children and their parents.

      • KCI등재

        인조혈관재료 표면에 도포된 혈관내피세포의 생리적 변화에 관한 연구

        이윤신,김용배 대한의용생체공학회 1996 의공학회지 Vol.17 No.2

        Antithrombogenic surFace is one of the most important things to the artificial vascular prostheses. This problem will be solved if the surface of prosthesis is covered with endothelial cells. The attachment and the growth of endothelial cells onto vascular prosthesis are very difficult. So many studies have been concentrated on the attachement of endothelial cell. But no good performance of the in uiwo experiments has been shown until now. In this study, we used the whole extracellular matrix (ECM) excreted from fibroblasts as an underlying matrix, and the endothelial cells were seeded to obtain the long term patency of vascular graft(i.e., for the patent 8 week implanted wafts in the animal model of rat). In order to study the antithrombogenic functions of cultured endothelial cells, prostaglandin(PGF 1 a) synthesis and platelet adhesion were assayed. The concentration of PGF a of stimulated group was sisnificantly higher than that of control group(21.97 $\pm$ 3.45 vs 4.93 $\pm$0.71 pg/1000 cells). The platelet adhesion of the polyurethane sheet covered with endothelial cells was lower than that of polyurethane sheet or sheet covered with ECM(1.04$\pm$0.28, 2.87$\pm$0.77, 2.89$\pm$0.70, % radioactivities, respectively). Endothelial cells grew well on polyurethane coated with ECM, synthesized the prostacyclin and functioned well as antithrombogenic. Therefore the endothelialization onto the ECM excreted from fibroblasts may be a good method for the vfudig prosthesis.

      • “온종일 활기찬 어린이집”을 위한 보육지원체계 개편

        이윤신 육아정책연구소 2019 육아정책포럼 Vol.62 No.-

        ’20년 3월부터 보육지원체계가 개편된다. 어린이집은 “기본보육”과 “연장보육”으로 보육시간을 구분하고, 시간대별 교사를 배치하여 기본보육시간에는 담임교사를, 연장보육에는 연장보육 전담교사를 배치할 수 있다. 기본보육에 보육료를, 연장보육에는 이용시간당 연장보육료를 지원하는 한편, 연장반 전담교사를 배치하면 인건비를 지원(신규로 채용하거나 보조교사가 전환·겸임하는 경우)한다. 보육지원체계 개편으로 교사의 근무여건을 개선하고, 장시간 보육이 필요한 아동의 수요에 적절히 부응하여 보육의 질을 높이고자 한다.

      • KCI등재

        공개 딥러닝 라이브러리에 대한 보안 취약성 검증

        정재한,손태식 한국정보보호학회 2019 정보보호학회논문지 Vol.29 No.1

        Deep Learning, which is being used in various fields recently, is being threatened with Adversarial Attack. In this paper,we experimentally verify that the classification accuracy is lowered by adversarial samples generated by malicious attackersin image classification models. We used MNIST dataset and measured the detection accuracy by injecting adversarialsamples into the Autoencoder classification model and the CNN (Convolution neural network) classification model, which arecreated using the Tensorflow library and the Pytorch library. Adversarial samples were generated by transforming MNISTtest dataset with JSMA(Jacobian-based Saliency Map Attack) and FGSM(Fast Gradient Sign Method). When injected into theclassification model, detection accuracy decreased by at least 21.82% up to 39.08%. 최근 다양한 분야에서 활용중인 딥러닝은 적대적 공격 가능성의 발견으로 위험성이 제기되고 있다. 본 논문에서는딥러닝의 이미지 분류 모델에서 악의적 공격자가 생성한 적대적 샘플에 의해 분류 정확도가 낮아짐을 실험적으로 검증하였다. 대표적인 이미지 샘플인 MNIST데이터 셋을 사용하였으며, 텐서플로우와 파이토치라이브러리를 사용하여만든 오토인코더 분류 모델과 CNN(Convolution neural network)분류 모델에 적대적 샘플을 주입하여 탐지정확도를 측정한다. 적대적 샘플은 MNIST테스트 데이터 셋을 JSMA(Jacobian-based Saliency MapAttack)방법으로 생성한 방법과 FGSM(Fast Gradient Sign Method)방식으로 변형하여 생성하였으며, 분류모델에 주입하여 측정하였을 때 최소 21.82%에서 최대 39.08%만큼 탐지 정확도가 낮아짐을 검증하였다.

      • KCI등재

        수술 후 첫 48시간 동안의 수술후 통증에 영향을 미치는 요인

        이윤신,손재순,윤혜상 기초간호학회 2014 Journal of korean biological nursing science Vol.16 No.3

        Purpose: This prospective study was designed to investigate the incidence of acute postoperative pain (APP) ≥4 and the risk factors of APP≥ for the first 48 hours after surgery. Methods: Data from 531 surgical patients were collected from November, 2009 to May, 2010. APP was assessed from the time of arrival at the Post Anesthetic Care Unit (PACU) to the end of the post-operative 48 hours. Risk factors of APP≥ were analyzed by logistic regression analysis. Results: The incidence of APP ≥4 was 58.8% for the first postoperative 4 hours; 33.5%, 24 hours; 11.1%, 48 hours. The score of pain was 5.55, the highest on arriving at PACU; 5.03 at postoperative 30 minutes; 4.03 at 1 hour; 3.96 at 4 hours; 2.76 at 24 hours; 1.44 at 48 hours Risk factors for APP ≥4 were females (Odds ratio [OR], 1.94; p=.013), general anesthesia (OR, 4.29; p<.001) and patient controlled analgesia (PCA) (OR, 2.83; p<.001) at 4 hours after operation; body mass index (BMI) ≥25 (OR, 1.80; p=.009), duration of surgery ≥1 hour (OR, 2.87; p=.037), general anesthesia (OR, 3.99; p<.001) and PCA (OR, 6.23; p<.001) at 24 hours; general anesthesia (OR, 3.53; p=.003) and PCA (OR, 3.01; p=.013) at 48 hours. Conclusion: Surgical patients with BMI ≥25, PCA and general anesthesia seem to have a higher incidence of pain ≥4 through the first postoperative 48 hours.

      • KCI우수등재

        임상간호사의 긍정심리자본과 조직공정성이 직무배태성에 미치는 영향

        이윤신,김희영 간호행정학회 2022 간호행정학회지 Vol.28 No.3

        Purpose: This study aimed to investigate factors affecting the job embeddedness of clinical nurses. Methods: Participants were 204 nurses working in four general hospitals. Data were collected from September 3 to October 8, 2020 and analyzed using the SPSS/WIN 25.0 program. Results: The mean scores for positive psychological capital, organizational justice, and job embeddedness were 3.31±0.38, 3.16±0.46, and 3.17±0.38, respectively. The job embeddedness of the participants had a positive correlation with optimism (r=.57, p<.001), hope (r=.56, p<.001), self-efficacy (r=.44, p<.001), and resilience (r=.38, p<.001) in the sub-factors of positive psychological capital. It also had a positive correlation with procedural (r=.58, p<.001), distributive (r=.52, p<.001), and interactional (r=.35, p<.001) justice in the sub-factors of organizational justice. The factors affecting nurses’ job embeddness were procedural justice, optimism, position, distributive justice, and hope. In addition, the explanatory power of the model was 60% (F=39.11, p<.001). Conclusion: The results suggest that to improve the job embeddedness, positive psychology-based coaching and counseling programs suitable for individual clinical nurses, organizational management through transparent procedures and objective and systematic distribution are needed. In addition, a position system suitable for the characteristics of a nursing organization is required.

      • KCI등재

        서울지역 일부 특급 호텔 조리종사자의 직무실태와 직업의식조사에 관한 연구

        이윤신,현영희 한국조리과학회 2000 한국식품조리과학회지 Vol.16 No.2

        A survey was carried out from 143 cooks working at the deluxe hotels in Seoul to obtain the information on the working environment, characteristics, and the satisfaction of the cooks to improve the culinary art training program and the working environment. The results were as follows: Most of the cooks(58.7%) worked for 8-9 hours/day and 51.7% of the cooks earned 1-1.5 million won in a month. The cooks had average 1.6 licenses per person, however, their licenses accorded with their work place only with 85.3%. Subjects were unsatisfied with the pay(55.2%), but 42.7% of the cooks hoped to work in their current work place. If they could transfer to other work place, they wished to run a restaurant of their own. The unsatisfaction rate was high among the cooks worked for 10-15 years, and 19.2% of them was unsatisfied with the environment of work place and 11.5% was in promotion. The longer the working period of the cook, the higher the recognition of the culinary skill. Also, the more frequently transfer to other places, the higher the self-estimation in their skills. Subjects answered that the most important factors for good cooking are the good taste and hygiene. The quality of ingredients was recognized more important among the cooks worked for longer period. The greatest hindrance for the improvement of cooking skill was indicated as insufficient knowledge among the cooks worked under one year, lack of confidence among those worked for 2-5 years, and authoritarianism of seniors for 6-15 years. They answered that the most important qualification for cook is the sincere attitude. The cooks with under 5 years of experience indicated experience and studying attitude and the ones with over 6 years of experience culinary skill as the important factors for cooks. The important factors for promotion was pointed out as culinary skill and human relationship.

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