인간 재조합 인터루긴-32 면역조절작용에 대한 유세포 분석

이광수,김영관,채정일,심정현,김은미,강형식,김수현,윤도영,명평근 충남대학교 생물공학연구소 2006 생물공학연구지 Vol.12 No.-

Xenotransplantation of porcine organs has the potential to overcome the severe shortage of human tissues and organ available for human transplantation. however, it remains various hurdles for clinical xenotransplantation. In pig and mouse xenotransplantation, porcine xenograft evoke a strong cellular rejection response in immunocompetent host and grafts are destroyed within a week. This cellular immune response could involved both T cells and NK cells. A number of groups have shown that human NK cells can recognize and damage porcine endothelial cells. In addition, human T cells can respond to porcine endothelial cells through both direct and indirect mechanisms. Cellular rejection of porcine tissues requires T cells, particularly CD4^(+) cells. A new cytokine recombinant human interleukin-32α,β(IL-32α,β) has a role innate and acquired immune system. In order to investigate the role of recombinant mouse IL-18 and recombinant human IL-32α,β in xenograft rejection, we transplanted the PK(15) cells to C57BL/6 mice with or without intraperitoneal injection of recombinant mouse IL-18 or recombinant human IL-32 α,β. It was analyzed the population of NK cell, T cell and B cell in the C57BL/6 mice transplanted with PK(15) cells and recombinant mouse IL-18 or recombinant human IL-32α,β by flow cytometry analysis. As a result, lymph node and thymus of PK15/IL18, PK15/IL32α and PK15/IL32β injected group were increased to T cell activation population than normal injected groups. CD8^(+) T cells were decreased in lymph node of PK15/IL18, PK15/IL32α and PK15/IL32β injected groups. CD4^(+) T cells were increased in lymph node cell of PK15/IL32α and PK15/IL32β injected group and also, B cell population were increased in lymph node cell and spleen of PK15/IL18, PK15/IL32α and PK15/IL32β injected group. Therefore, we suggest that recombinant mouse IL-18 and recombinant human IL-32α,β suppress xenograft rejection in cellular xenotransplantation.

통풍성 관절염의 임상적 고찰

윤채중,정승문,김영학,김동규,허광식,김태원,배학연,정종훈,이승일,김평남 朝鮮大學校 附設 醫學硏究所 1997 The Medical Journal of Chosun University Vol.22 No.2

통풍은 Purine 대사의 이상으로 발생하는 질환으로, 고뇨산혈증인 사람의 전부가 통풍으로 발현되지 않고 증상의 출현 양상이 다양하여 진단과 치료에 주의가 필요하며, 조기에 적절한 조치를 한다면 충분히 조절이 가능한 질환이다. 본대학 내과학교실에서는 통풍으로 치료한 32명의 환자에서 임상양상, 병력과 검사소견을 분석하여 다음과 같음 결과를 얻었다. 1. 32명 모두 남자이며, 최초 발병 시기는 24세에서 72세로 평균 43.8±11.9세이었으며, 30대에서 40대까지가 19명(59.4%)으로 대부분을 차지하였다. 내원 당시 나이는 27세에서 75세까지로 평균 52.3±10.4세였으며, 내원시 까지 평균 유병기간은 8.5±6.8년으로 나타났다. 2. 동반 질환으로는 고지혈증 12례, 신장질환 10례, 고혈압 12례, 비만 8례, 당뇨 2례 등이었다. 3. 이환된 관절은 단관절 침범이 19례(59.4%), 다관절 침범이 13례 이었으며, 최초 이한된 관절은 족무지 중족골지골 관절로 19례(59.4%)로 가장 많았고, 통풍 결절은 20례(62.5%)에서 관찰되었으며, 유병기간이 10년 이상된 12례중 11례 (91.7%)에서 결절이 관찰되었다. 4. 평균 혈중 요산치는 9.17±1.75 ㎎/dl이었으며, 8.0 ㎎/dl에서 9.9 ㎎/dl 사이가 19명으로 전체의 59.4%를 차지하였다. Objective: The gout is a heterogeneous group of diseases resulting from tissue deposition of monosodium urate or uric acid crystals from extracellular fluids supersaturated with respect to this end product of human purine metabolism. The clinical manifestations are such as hyperuricemia, gouty arthritis, gouty nephropathy, uric acid nephrolithiasis. We analyze of clinical manifestations and associated factors in gout. Method: We have reviewed the medical records, radiologic findings and clinical results of thirty-two patients admitted at our department from April 1996 to July 1997. Result: 1) All patients were male. The mean age at initial attack was 43.8 years old, ranging from 24 to 72 years old. 2) The mean level of serum uric acid was 9.17mg/dl on admission. 3) The first metatarsopharyngeal joint was involved in 19 cases (59.4%). Tophus was observed in 20 cases (62.5%). 4) Hyperuricemia was associated with hypertension, obesity, nephrolithiasis and hyperlipidemia.

토끼의 Aflatoxin B₁중독증에 대한 인삼엑기스의 효과 : 임상, 생리적 및 병리학적 변화 Clinical, Physiological and Pathological Changes

김차용,변유성,문평일,정덕화,김종수 慶尙大學校 1987 論文集 Vol.26 No.1

Chinchilla 계통 1~1.5㎏ 되는 토끼 30마리를 5군 으로 나누어 체중㎏당 제 1군은 대조군으로서 Cornoil 0.3ml 씩 제 2군은 aflatoxin B? 0.03mg과 인삼 엑기스 8mg을 제 5군은 aflatoxin B? 0.06mg과 인삼엑기스 8mg을 3주동안 카테타로 경구 투여하여 임상, 혈액학적 및 병리학적 변화를 관찰하였다. 1.사료소비량 감소, 침울, 쇠약, 혼수, 체중증가율감소등과 같은 임상증상은 대조군을 제외한 전 처리군에서 독을 투여후 3일째 부터 전처리기간을 통하여 나타났으나 aflatoxin B? 단독처리군에서 인삼엑기스 혼합처리군보다 더 현저하게 나타났다. 2. 혈청 효소와 AST, ALT는 독물투여후 7일부터 인삼엑기스 혼합투여군 보다 aflatoxin B? 단독 투여군에서 높게 증가하였다. 3.전처리군에서 적혈수용적, 헤모글로빈농도는 영향을 받지 않았고, 혈장단백질은ㅇ 독물투여후 14일이후 aflatoxin B?단독 처리군에서 현저하게 감소하였다. 4. Aflatoxin B?과 인삼엑기스 혼합 투여군 보다 aflatoxin B? 단독투여군에서 간장의 공포변성,담관증생, 핵농축, 붕괴, hepatic fibrosis와 임파절 및 비장에서 현저한 임파양 세포의 소실을 나타내었다. The present study has been carried out to investigate the effectsof ginseng extract on induced chronic aflatoxicosis in rabits. Groups of rabbits were treated orally with aflatoxin B? and mixture of ginseng extract for 21 days with a dosage of 0.03mg, 0.06mg of the toxin and 0.03mg or o.o6mg of the toxin mixture of 8mg of ginseng extract or 0.3ml corn oil s place to per kg body weight. The results obtained were is follows. 1. Sings o toxicosis included reduction of feed consumption emaciation, decrese of body weight and coma revealed in both groups treated with 0.03mg pr 0.06,mg of aflatoxin B?/Kg/B.W than groups 4.5 after days 3. 2. In groups 2, 3(aflatoxin B? 0.03, 0.06mg), Activity of asparate aminotransferase(AST) and alanine aminotransferase (ALT)were increased sigificantly in dats7, 14 and 21. 3. In all groups, Mean values for packed cell volume(PCV)and hemogobin were unremarkasble, but plasma total protein were decreased in groups 2,3than 4,5 groups on days 14 and 21. 4. The rabits treated with aflatoxin B?(2,3 groups)showed markedly degenerative chasnges ranging from swelling and decreaseed granularity to marked vacuolation, hepatic necrosis, fibrosis and bile duct, Proliferation and depletion of lymphocytes in lymphocytes in lymphonde, spleen than groups 4,5.

살충성 O,O-Dimethyl-O-(3-Metyl-4-Nitrophenyl)-phosphorothioate (Sumithion^�)의 전기화학적 환원반응에 미치는 Micell의 영향

成洛道,明平根,朴勝熙,金日光 圓光大學校 基礎自然科學硏究所 1988 基礎科學硏究誌 Vol.7 No.3

The electrochemical reduction of sumithion in various surfactants, NaLS, CTABr, Triton X-100 and in acetonitrile solution has been examined by DC, DP polarography and cyclic voltammetry (CV). Especially, in anionic surfactant, NaLS solution, the height of reduction wave is dramatic eliminated and half-wave potentials are shifted to strong negative potential (-2.7 volt vs. Ag-AgCl) by repulsion of nitro group in sumithion and anionic micell surfaces. The processes of reduction of sumithion were irreversibly electrochemical mechanism and the result of the reaction at high cathodic potential (-2.7v. vs. Ag-AgCl), O,O-dimethyl-O-(3-methylhydoxyaminophenyl) phosphorothioate is formed as major product via O,O-dimethyl-O-(3-methylnitrosophenyl) phospho-rothioate in NaLS micell solution.

살충성 O,O-Dimethyl-O-(3-Methyl-4-Nitrophenyl)-Phosphorothioate (Sumithion^�)의 전기화학적 환원반응에 미치는 Micell의 영향

成洛道,明平根,朴勝熙,金日光 圓光大學校 基礎自然科學硏究所 1987 基礎科學硏究誌 Vol.6 No.3

The electrochemical reduction of sumithion in various surfactants, NaLS, CTABr, Trition X-100 and in acetonitrile solution has been examined by DC, DP polarography and cyclic voltammetry(CV). Especially, in anionic surfactant, NaLS solution, the height of reduction wave is dramatic eliminated and half-vale potentials are shifted to strong negative potential (-2.7 volt vs. Ag-AgCl) by repulsion of nitro group in sumithion and anionic micell surfaces. the processes of reduction of sumithion were irreversibly electrochemical mechanism and the result of the reaction at high cathodic potential (-2.7 volt vs. Ag-Agl) by repulsion of nitro group in sumithion and anionic micell surfaces. The processes of reduction of sumithion were irreversibly electrochemical mechanism and the result of the reaction at high cathodic potential (-2.7 volt vs. Ag-AgC1), O,O-dimethy1-O-(3-methylhydoxyaminophenyl) phosphorothioate is formed as major product via O,O-dimethyl-O-(3-methylhydoxyaminophenyl) phosphorothioate in NaLS micell solution.

살충성 O,O-Dimethyl-O-(3-Methyl-4-Nitrophenyl)-phosphorothioate (Sumithion^�)의 전기화학적 환원반응에 미치는 Micell의 영향

成洛道,明平根,朴勝熙,金日光 충남대학교 약학대학 의약품개발연구소 1986 藥學論文集 Vol.2 No.-

The electrochemical reduction of sumithion in various surfactants, LaLS, CTABr, Triton X-100 and in acetonitrile solution has been examined by DC, DP polarography and cyclic voltammetry (CV). Especially, in anionic surfactant, NaLS solution, the height of reduction wave is dramatic eliminated and half-wave potentials are shifted to strong negative potential (-2.7 volt vs. Ag-AgCl) by repulsion of nitro group in sumithion and anionic micell surfaces. The processes of reduction of sumithion were irreversibly electrochemical mechanism and the result of the reaction at high cathodic potential (-2.7 v. vs. Ag-AgCl), O,O-dimethyl-O-(3-methylhydoxyaminophenyl) phosphorothioate is formed as major product via O,O-dimethyl-O-(3-methylnitrosophenyl) phosphorothioate in NaLS micell solution.

Rhizobacteria-induced Resistance Perturbs Viral Disease Progress and Triggers Defense-related Gene Expression

Il-Pyung Ahn,Kyungseok Park,Choong-Hoe Kim 한국분자세포생물학회 2002 Molecules and cells Vol.13 No.2

Selected strain of nonpathogenic rhizobacterium EXTN-1 from the Bacillus amyloliquefaciens is capable of eliciting broad-spectrum induced systemic resistance (ISR) in several crops that is phenotypically similar to pathogen-induced systemic acquired resistance (SAR). In tobacco (Nicotiana tabacum cv. Samsun- nn), EXTN-1 treatment also perturbs the disease progress by Pepper mild mottle virus (PMMoV), a member of Tobamovirus group. To investigate the defense mechanisms induced by this rhizobacterium, expression patterns of defense-related genes were analyzed. The EXTN-1-treated tobacco plants showed augmented, rapid transcript accumulation of defenserelated genes including PR-1a, phenylalanine ammonia- lyase (PAL), and 3-hydroxy-3-methylglutaryl CoA reductase (HMGR) following inoculation with PMMoV. This was the typical phenomenon of potentiation. Accelerated expression of all these genes was subsequently detected in the noninoculated, upper leaves; thus, their expression is associated with the development of both local and systemic resistance. Coordinated reduction of viral genome accumulation was clearly detected in the leaves of tobacco pretreated with EXTN-1. EXTN-1 treatment on Arabidopsis wild type Col-0 resulted in the activation of PR-1 and PDF1.2 at the same time. All these results may indicated that EXTN-1 induces systemic resistance via salicylic acid- and jasmonic acid-dependent pathways and timely recognition followed by rapid counterattack against the viral invasion is the key differences between incompatible interaction and compatible one.

성인에서의 담관 낭종과 췌담관 합류 이상

김명환,김영환,김효정,이성구,서동완,최호순,이승규,유병무,민영일,고문수,민병철,이미화,임미경,김표년 대한소화기내시경학회 1996 Clinical Endoscopy Vol.16 No.1

Background/Aims: Choledochal cyst is a congenital dilatation of extraor intrahepatic bile duct . Recently, anomalous union of pancreaticobiliary duct(AUPBD) is supposed to be one of the major causes of choledochal cyst. The aims of this study are to assess the association ratio of AUPBD in choledochal cyst and whether the AUPBD in choledochal cyst have a clinical significance. Methods: The study subjects were 35 adult patients who were diagnosed by ERCP in Asan Medical Center from August, 1990 to July, 1995. We have classified the type of choledochal cyst and AUPBD according to Todani and Kimura, respectively. Result: The number of choledochal cyst was 14 cases(40%) in type I, I case(3%) in type II, 6 cases(17%) in type Ⅲ, 11 cases(31%) in type IVA. Three cases were unclassified. Among the 35 cases, 22 cases(63%) of choledochal cyst were associated with AUPBD. AUPBD was only seen in type I, IVA and unclassified form of choledochal cyst. The number of patients with type 1 AUPBD was 10(45%) and type 2 was 12(55%) by Kimura's classification. 'Che mean age was younger in patients with choledochal cyst and AUPBD(36.2 16 years) than in choledochal cyst aione(49.3 19 years). Acute inflammation and malignancy of biliary tree 8z pancreas were more commonly seen in patients with choledochal cyst and AUPBD than choledochal cyst alone. Conclusion: In patients with choledochal cyst, the association of AUPBD was not uncommon and they had more grave clinical course. AUPBD may be a contributing factor to develop mali~ancy in patiehts with choledochal cyst.

Intranasal Delivery of HMGB1 siRNA Confers Target Gene Knockdown and Robust Neuroprotection in the Postischemic Brain

Kim, Il-Doo,Shin, Joo-Hyun,Kim, Seung-Woo,Choi, Sunghyun,Ahn, Junseong,Han, Pyung-Lim,Park, Jong-Sang,Lee, Ja-Kyeong Elsevier Science B.V., Amsterdam 2012 Molecular therapy Vol.20 No.4

韓國際政治에 있어서 中共의 位置

金一平(Il Pyung Kim),安秉俊(Byung Choon Ahn) 한국국제정치학회 1965 國際政治論叢 Vol.4·5 No.-