국내의 양식산 무지개송어 성어로부터 분리된 IHNV의 G protein gene에 대한 연구

김기홍,김위식,김춘섭,김영진,정태성,신이치, 키타무라,정성주,오명주 한국어병학회 2003 한국어병학회지 Vol.16 No.3

An Infectious hematopoietic necrosis virus strain (IHNV-RTK) was isolated from cultured rainbow trout at Kumi in Korea during 2000 and 2001. In the RT-PCR amplification with the specific primer set designed from IHNV G protein region, a 540 bp PCR product was amplified from the RTK strain. The RTK strain showed higher sequence homology with the published IHNV G protein genes (RB-76, LR-73, Col-85, and Carson-89).

PCR을 이용한 Rat 기관지 세척액에서의 Pneumocystis carinii DNA의 검출 : 기초실험 A pilot study

김주옥,홍석철,한표성,이종진,김선영,이영하,신대환 충남대학교 의과대학 지역사회의학연구소 1994 충남의대잡지 Vol.21 No.1

Background: Pneumocystis carinii (PC) is a opportunistic pathogen causing serious pneumonia (Pneumocystis carinii pneumonia : PCP) in immunosuppressed patients including AIDS. Laboratory diagnosis of PCP is dependent on microscopic demonstration of the PC by using cytochemical staining or by immunocytochemical staining with monoclonal antibodies. However, these staining methods are not highly sensitive. The development of the polymerase chain reaction (PCR) has made it possible to detect very small numbers of pathogens in clinical specimens. To establish the usefulness of PCR for detection of PC DNA, We performed PCR as a pilot study. Methods: PCP was induced on Spraque-Dawley rats with prednisolone 5mg IM twice a week. Bronchial lavages, impression smears, and permanent sections were performed from the 3rd week to 7th week of cortisonized rats. PC DNA was extracted with bead beater / 10% CTAB method. Results: Impression smears showed PC cyst after the 4th week (100%, 28/28 rats), and permanent sections showed 27/28 rats. Control rats showed some PC cysts (2/7). PCR result was positive only one case among the 6th week rats (1/7), But positive 6 cases at the 7th week rats, (6/7). Among control rats. 2 cases were positive (2/7). ??This data is a pilot study for the PC DNA detection using a PCR. PCP were successfully cortisonized rats. If adequate bronchial washing technique will be used, PCR can be a sensitive for PC DNA detection.

新農村建設(定住生活圈開發)을 위한 理論的 考察 및 開發需要調査

金鴻允,李弘柱,鄭禮杓,全禹亭,趙興洙,李信昊,全榮吉 안성산업대학교 1992 論文集 Vol.24 No.-

Rural of Koreas has rapidly changed through the process of industrialization and urbanization. During past 30 years, development differences between urban and rural, metroplitan area and area derived from a highly economic growth, bring about dual specialization that deepens overpopulation and sparsity. Therefore, with a new concern about rural, necessity of rural development policy that developes a settlement and life area for settlement for ensuing stablized settlement and living activity of rural people, has been drived step by step. For rural welfare establishment that is settled and good hereafter, this study aims at attempting a theoretical approach about the way of development for rural settlement as a new rural development strategy through examination of today's rural reality, under assumption that rural people must be able to obtain stable jobs and to be pleasant without leaving rural area, and rural settlement to be able to enjoy urban advantages must be realized. Therefore, items that must be obtained basically rural area to be a human settlement space are equipment connected with a dwelling house, production, consumption, job relaxation, infrastructure connected with transportation or communication network and environment. By the way, the best function of a human settlement space makes a basic pleasant settlement area to be in the middle of that. Then rationalization of land use must be realized as being a best condition for human life around space of a dewelling house. Besides, spacial accessibility about all equipment of inhabitants must be easy. That is, economic distance, social distance and cultural distance as well as spacial distance must be included. Also, those spacial functions must be realized on a basis of suitable enironment for a human settlement life. At the same tiem, the upper area must be connected with the lower area as the system of spacial settlement in settlement space. But truely, as rural people can't satisfy with services of the surrounding rural areas, they desire better city or metroplitan. The these problems bring about inequality between urban and rural. Therefore, here are urgent reasons that must be done rearing of rural central town and rural integrated development of unit area. So, these dys, to correct maldistribution of land use and inequality of settlement space that Korean society faces on, new strategies of rural integrated development for lift area for settlement than gives the meaning. That rural areas become like a place for human settlement life will be able to be chosen in the view of humanism. The say of thinking of rural residents(Jukans-myun, Anseong-gun, Kyeonggi-do) was surveyed in order to develop new rural settlement planning. From the data collected in this survey, rural life-style, view on traditional rural life, agriculture, farming, agri-policy, social attitude, and degree of satifaction of rural life, etc, were analyzed. Firstly, most of rural residents interviewed in this survey have the opinion of not leaving their home village if living conditions improved to a reasonable level in the near future. In decision-making process of village affairs, they want to participate actively and equally. Secondly, in spite of their acceptance of agriculture as a vital sector destined to national security, most of them think that framing should be little of promise. Thirdly, they have the negative attitude to urban-oriented social valued and also to the agricultural policy, while seeking consolation through human and traditional viewing of rural life style. Therefore, in the development of new rural settlement model, idea-making should be directed to the betterment of communal value of village life, stability of rural economy, rationalization of rural life style and enhancement of rural-type happiness.

다량의 하부위장관출혈이 병발된 Vibrio vulnificus 패혈증 1예

최주연,유진홍,한석원,이대훈,최민호,박순민,김연식,김선우,신완식,강문원,강성구 대한화학요법학회 1996 대한화학요법학회지 Vol.14 No.2

최근 저자들은 Vibrio vulnificus 감염에 의해 패혈증 및 이차성 피부 병변을 보인 42세의 남자 환자의 치료중 하부 위장관 출혈이 병발된 1예를 경험하였기에 보고하는 바이다. We experienced a case of Vibrop vulnificus septicemia which showed an unusual manifestation such as hematochezia. The patient was a 42-year-old man with chronic liver disease. The blood and wound culture revealed the organism. During intensive care, he suffered from massive hematochezi for several days. Colonoscopy and abdominal CT showed findings suspicious of ischemic colitis. To our knowledge, this is the first unusual case of V.vulnificus infection which showed massive lower gastrointestinal bleeding.

양식산 터봇, Scophthalmus maximus에서의 Irido-like virus 감염

김위식,김기홍,김춘섭,김영진,정성주,정태성,신이치, 키타무라,마모루, 요시미즈,오명주 한국어병학회 2003 한국어병학회지 Vol.16 No.3

본 연구는 병리조직학적 및 전자현미경적 방법을 통해 양식산 turbot에서 발생하는 irido-like virus의 감염증을 소개하고자 한 것이다. 2003년 6월 전북 고창 소재 육상수조에서 양식중인 turbot치어에서 50∼70%의 대량폐사가 발생하였다. 병어는 사료섭취가 저하되고 힘없이 유영하다 폐사되었으며, 죽기 직전의 어류는 복부가 팽만되고 안구가 돌출되며 체색이 옅어지는 증상을 보였다. 해부학적 조사 결과, 아가미 빈혈과 간이 퇴색되어 있었으며, 특히 비장조직이 심하게 비대되어 있었다. 병어에 대한 조직학적 검사에서 비대 세포를 특징으로 하는 특이 병변이 주로 신장과 비장의 조혈조직에서 관찰되었으며, 아가미, 심장, 위, 장, 간, 췌장, 지방 조직에서도 관찰되었다. 그리고 전자현미경적 관찰 결과, 비대 세포의 세포질내에서 육각형의 지름이 136∼159 ㎚인 Irido-like virus를 관찰할 수 있었다. The high mortality of cultured juvenile turbot, Scophthalmus maximus occurred in Gochang on June, 2003. The diseased fish was lethargic with reduced feed intake. Grossly, these fish showed pale body, abdominal extension and exophthalmia. The dominant internal gross features of diseased fish were severely enlarged spleen, pale gills and or liver. Diseased fish histologically showed basophilic enlarged cells in the kidney, spleen, gill, heart, stomach, intestine, liver, pancreas and adipose tissue. Transmission electron microscopy (TEM) reveled hexagonal virions in the cytoplasm of necrotic cells. The viral particles lead a central electron-dense core and an electron translucent zone, and were 136-159 nm in diameter. These results suggest that the virus belonging to the iridoviridae was responsible for the mortality of cultured juvenile turbot.

물결합재비 및 단위수량에 따른 고유동콘크리트의 제공학적 특성에 관한 실험적 연구

김규용,한장현,신동주,신홍철,김무한 忠南大學校 産業技術硏究所 1998 산업기술연구논문집 Vol.13 No.1

Recently the study on high flowing concrete which has high workability and non-compacting is being proceeded actively in the university and corporative laboratory. There are some cases that has been applied to the field. This high flowing concrete has higher fluidity and segregation resistance than Plain or flowing concrete. And it is being focused as a remarkable know-how which can make high-quality concrete and reduction effect of labor force. This properties of high flowing concrete are influenced by the relationship of several factors; binder content water binder ratio and unit water content. It is the aim of this study to propose reference data at mix design of high flowing concrete, after comparing and analyzing the fluidity and strength properties of high flowing concrete according to water binder content ratio and unlit water content.

황정(黃精) 추출물의 화학구조결정에 관한 연구 (Ⅰ)

윤중호,박주희,김정주,권기락,안철진,주우홍,강진호,신동수 Institute of Genetic Engineering Changwon National 1998 Gene and Protein Vol.2 No.1

본 연구에서는 황정 속에 포함되어 있는 생리활성 물질을 hexane, CHCl₃과 n-butanol층에서 각각의 성분들을 추출하였고, hexane층에서 분리된 화합물 I과 H 중에서, 화합물 I의 봐학구조를 ¹H-nmr, ??, DEPT135, COSY, HMQC, HMBC 스펙트럼 및 MS 스펙트럼 등의 분광학적인 방법에 의해 결정하였다. 화합물 I의 구조는 9,12-(9E, 12E)-octadecadienoic acid 임을 확인하였다. In this Paper, biologically active compounds were extracted using organic solvents as hexane. CHC1₃, n-butanol to give each component. Chemical structure of compound I was characterized using ¹H-nmr, ??, DEPT135, COSY, HMQC, HMBC spectrum and MS-spectrum, in separated compound I and Ⅱ from hexane layer. Finally, chemical structure of compound I was determined as 9,12-(9E,12E)-octadecadienoic acid.

Flavobacterium meningosepticum에 의한 균혈증 1예

장성원,유진홍,진승원,김선우,김연식,박순민,이대훈,최민호,최주연,신완식,강문원,강지민 대한화학요법학회 1997 대한화학요법학회지 Vol.15 No.1

1996년 6월 카톨릭대학교 부천성가병원에 뇌경색으로 입원했던 61세 남자 환자의 혈액에서 Flavobacterium meningosepticum을 분리하였다. 환자는 폐렴을 동반한 패혈증의 예로 ceftriaxone과 metronidazole을 투여하였으나 입원 48시간만에 사망하였다. Flabovacterium meningosepticum is a rae cause of nosocomial infection which shows multi-drug resistance. It mainly invades patients with impaired immunity. Recently, we experienced a case of F. meningosepticum bacteremia in a patient with chronic debilitated state owing to stroke. The clinical progress was so rapid that he died within 48 hours. Blood culture revealed F. meningosepticum which showed characteristic yellow colony in blood agar plate.

동아를 이용한 미용식품 및 화장품 개발 : 1. 멜라닌 생성 억제와 여드름균 억제에 미치는 동아 추출액의 효과

안용근,김승겸,신철승,민주홍 한국식품영양학회 2002 韓國食品營養學會誌 Vol.15 No.2

고형분 함량은 어린 동아 착즙에서는 3.1%, 익은 동아 착즙액에서는 0.1%를 나타냈고, 주성분은 당으로 89.7%를 나타냈다. 팩틴 함량은 익은 동아즙은 4.11mg/ml, 어린 동아즙은 4.43mg/ml이었다. 당 함량은 익은 동아 육질은 sucrose 0.1%, glucose 0.32%, fructose 0.35%, 미확인당 1이 0.06%, 2가 0.04%로 합계 0.87%를 나타냈다. 어린 동아 육질은 sucrose 0.33%, glucose 1.04%, fructose 1.12%, 미확인당 1이 0.18%, 2가 0.12%로 합4계 2.79%를 나타냈다. pH는 익은 동아는 4.64, 육질은 4.94, 어린 동아의 속은 4.96, 육질은 5.40을 나타냈다. 유기산은 익은 동아 육질에는 citric acid 0.409, malic acid 0.084, succnic acid 0.048%, 속에는 citric acid 0.648, malic acid 0.127, succinic acid 0.057%, 어린 동아 육질에는 citric acid 0.028, malic acid 0.219, succinic acid 0.298%, 속에는 citric acid 0.039, malic acid 0.360, succinic acid 0.224% 들어 있다. Fumaric acid는 미미하다. 총량은 익은 동아 속이 0.833, 어린 동아속 0.624, 어린 동아 육질 0.546, 익은 동아 육질 0.541이었다. 여드름균(Propionibacterium acnes)에 대한 항균력은 대조군이 9(Ø, cm)인데 반해, 비열처리 동아즙은 2.6, 열처리 동아즙은 2.5, 1/5 농축한 것은 1.9, 1/10 농축한 것은 2.5, 동결건조한 것은 2.3을 나타냈다. 멜라닌 생성 억제력은 0.01%에서 대조군이 29 ㎕/ml인데 반해 열처리 동아즙은 15 ㎕/ml을 나타냈고, 비열처리 동아즙은 이보다 약간 높았다. 1/5 농축한 것은 24 ㎕/ml, 1/10 농축한 것은 23 ㎕/ml, 동결건조한 것은 19 ㎕/ml를 나타냈다. 이들 결과에 따라 동아즙을 30% 가하여 나리싱크림과 밀크로숀을 조제하였다. Lyophilizate of immatured wax gourd extract was 3.1%, matured wax gourd extract was 1.0%, and its main ingredient was sugar, which accounts for 89.7% in total residue. In matured wax gourd, pectin contents was 4.1 lmg/ml, and in immatured wax gourd 4.43mg/ml. In matured wax gourd sarcocarp, sugar contents was 0.1% of sucrose, 0.32% of glucose, 0.35% of glucose, 0.35% of fructose, the first unidentified sugar was 0.06% and the second was 0.04%, and all total 0.87%. In sarcocarp of immatured wax gourd, sucrose was 0.33%, glucose was 1.04%, frutcose was 1.12% and the first unidentified sugar 0.18%, and the second was 0.12%, which total 2.79%. In matured wax gourd core, pH was 4.64, sarcocarp 4.94, immatured wax gourd core 4.96, sarcocarp 5.40. According to the organic acid analysis, in sarcocarp of matured wax gourd, citric acid of 0.409 was contained, malic acid 0.084, succnic acid 0.048%, in matured wax gourd core, citric acid was 0.648, malic acid 0.127, succnic acid 0.058%, in immatured wax gourd, citric acid 0.023, malic acid 0.219, succinic acid 0.298%, in immutured wax gourd, citric acid was 0.039, malic acid 0.350, succinic 0.224%. Fumaric acid was trace in all cases. Total organic acid in matured wax gourd core was 0.833, immatured wax gourd core wax 0.624 and immatured wax gourd sarcocarp was 0.546, matured wax gourd sarcocarp was 0.541%. In inhibition rate to Propionibacterium acnes, control was 9 (Ø, cm) was gourd that was not heated was 2.6 and wax gourd which was heated was 2.5, concentrated by 1/5 was 1.9, wax gourd by 1/10 was 2.5, freezing dry was 2.3. Wax gourd which not heated on producing melanin in B-16 melanoma cell, the melaning forming unit was 15㎕/ml in addition of 0.01%, while that as a control was 29 ㎕/ml. The unit from heated was gourd shows little lower value than that heated. The concentrate by 1/5 was 24 ㎕/ml. The extract by 1/10 was 23 ㎕/ml, freezing dry was 19 ㎕/ml. Milk lotion and nursing cream were made with the addition of 30% was gourd according to the above results.

새터민의 기초직업능력 진단

이찬,이용환,이윤조,신재호,홍윤선,최홍주 한국농업교육학회 2007 농업교육과 인적자원개발 Vol.39 No.3

이 연구는 새터민의 고용현황 및 문제점을 고찰하고 새터민의 기초직업능력을 진단하여 직업능력개발 방향에 있어 시사점을 제공하는데 그 목적이 있었다. 새터민의 기초직업능력 진단을 위한 도구는 관련 문헌 고찰과 pilot test 및 새터민 교육ㆍ지원 담당자의 안면타당도 검증 등을 통하여 개발되었다. 연구의 목적을 달성하기 위하여 현재 하나원에서 사회 적응교육 및 직업교육을 받고 있는 새터민의 기초직업능력을 진단함으로써 우리나라에 거주하는 새터민의 기초직업능력을 파악하였다. 주요 결과는 다음과 같다. 첫째,새터민들의 기초직업능력 중에서 직업윤리능력은 상당히 갖추어져 있는 것으로 인식하고 있으며,정보능력,즉 컴퓨터 사용능력은 상대적으로 낮은 수준으로 평가하고 있다. 둘째,성별에 따라서는 기초직업능력에 있어서 통계적으로 유의미한 차이를 보이지 않았다. 연령에 따라서는 정보능력과 수리 능력,조직이해능력 영역에 있어서 통계적으로 유의미한 차이를 보였다. 셋째,학력에 따라서는 문제해결능력과 의사소통능력,수리능력,대인관계 영역에 있어서 통계적으로 유의미한 차이를 보였다. 넷째,새터민의 취업을 위한 기초직업능력 개발에 있어 정보능력,수리능력,의사소통능력의 비중 확대,수준별ㆍ맞춤형 교육프로그램개발,새터민을 위한 취업 및 사업정보 제공 등을 제시하였다. The purpose of this study was to explore the current employment status and HRD issues of North Korean refugees and to assess their level of the basic vocational competencies. The assessment tool for the basic vocational competencies was developed based on literature reviews and pilot tests from the North Korean refugees. The face validity was verified by educators and counselors for the North Korean refugees. The major findings in this study were as follows: First, among the basic vocational competencies of the North Korean refugees, the level of vocational ethics was the highest and the level of information teachnology competency was the lowest. Second, the North Korean refugees' basic vocational competencies showed no statistical differences by gender. Territories of information competency, mathematical competency, and communication competency showed statistical differences by age. Third, territories of problem-solving competency, communication competency, mathematical competency, mathematical competency, and communication competency showed statistical differences by education level. Fourth, to develop the basic vocational competencies for North Korean refugees, the following suggestions were presented.; 1) extend the importance of information technology, mathematical, and communication competencies,; 2) develop the customized training programs by competency level,; and 3) offer the employment information for North Korean refugees.