성장호르몬 분비성 뇌하수체 선종에서 소마토스타틴 수용체 (제2아형, 제5아형), G_i2α 및 Pit-1 유전자 발현

류미숙,양인명,박철영,우정택,김성운,김진우,김영설,최영길,김은희,박승준,김국기 대한내분비학회 2002 Endocrinology and metabolism Vol.17 No.2

Background: Mutation of Gs protein subunit (gsp oncogene), detected in about 30∼40% of growth hormone (GH)-secreting pituitary tumors, is associated with an increased long-acting somatostatin analog octreotide sensitivity. However, the mRNA expression of somatostatin receptor (sst) was not changed in the GH-secreting pituitary tumor, regardless of whether they were gsp oncogene positive or negative. This suggests that the expression of genes coding for G_i2α, Pit-1 and the other factors involved in the regulation of secretory activity in somatotrophs is likely to be altered in gsp oncogene positive tumors. We observed the impact of the gsp oncogene on the expression of the genes coding for Gi2, Pit-1 and sst (2&5) in GH-secreting pituitary tumors. Methods: The GH response to octreotide was examined in 13 acromegalic patients before transsphenoidal adenomectomy. Genomic DNA and RNA were extracted from fresh frozen tumor tissues. PCR was performed to amplify and sequence the region between codon 184 and 251 that includes exons 8 and 9 of the Gs gene. Sst2, sst5, G_i2α and Pit-1 mRNA levels were measured by semi-quantitative RT-PCR. Results: Sst2 and sst5 mRNA transcripts were detected in all tumors (7 gsp +, 6 gsp-). The amount of sst transcripts varied considerably varied between the tumors. There were no significant differences in sex, age, tumor size, grade or basal GH levels. Pit-1 and sst2 mRNA levels were not different. In contrast, G_i2 mRNA levels were significantly higher in gsp (+) while sst5 mRNA levels were higher in gsp (-). Conclusion: These data suggests that gsp oncogene may increase Gi2α levels but decrease sst5 mRNA levels. However, Pit-1 and sst2 mRNA expression may not be affected by gsp oncogene. The increased expression of the G_i2α gene might be an inhibitory compensatory response to the action of gsp oncogene

성인 막성신병증의 임상적 고찰

양종오,이상주,박기현,장윤경,이강욱,서광선,신영태 충남대학교 의학연구소 2001 충남의대잡지 Vol.28 No.2

Membranous nephropathy (MN) is a glomerular disease characterized by diffuse thickening of the glomerular basement membrane without significant mesangial proliferative change and is usually manifested by the nephrotic syndrome. To evaluate the clinical and pathologic characteristics of this disease in adults, we analyzed 65 patients with primary membranous nephropathy who were diagnosed at Department of Internal Medicine, Chungnam National University Hospital from February 1986 to February 2001. The results are as follows: 1) Of total 96 patients with membranous nephropathy, 67.7% was primary MN. Hepatitis B-associated MN and lupus MN were 22.9%, and 9.4%, respectively. Median duration of follow-up was 35.1 months(0.3∼177) in primary MN, 49.6 months(2.5∼103) in lupus MN, and 35.6 months(0.5∼108) in hepatitis B-associated MN. 2) Mean age of the patients with primary MN at the time of diagnosis(43.3 years) was significantly higher than that of lupus MN(30.4 years) and HBV-associated MN(36.2 years). Male to female ratio of primary MN was 1.03:l. 3) At the time of diagnosis, 89.2% of primary MN was presented with nephrotic syndrome. Twenty seven percent of patients showed hypertension. Hematuria was found in 58.5% of primary MN patients. Azotemia was noted in 3.1 %. 4) Global sclerosis, tubular atrophy and interstitial fibrosis were noted in 39.8, 16.1 and 11.3% of primaty MN, respectively. IgG and C3 were deposited on the glomerular capillary loop in 92.7% and 29% of primary MN patients. There was no significant correlation between the electron microscopic pathologic stage and clinical findings. 5) Of 56 patients with primary MN followed more than 6 months, 50(90.9%) patients were treated with prednisolone, cyclophosphamide or cyclosporin. Complete remission was obtained in 29 patients(51.8%), partial remission in 6 patients(10.7%), and no response in 18 patients(32.1%). Of 6 patients who were not treated with immunosuppressive drugs, 3 patients showed spontaneous complete or partial remission. Three patients who did not show clinical improvement progressed to end-stage renal failure. There were no significant clinical difference including renal pathology at the time of diagnosis between the patients who showed complete or partial remission and patients who did not. In conclusion, it was difficult to predict the long-term prognosis of primary MN at the time of diagnosis, clinically. The response to therapeutic modality would be the most important to predict long-term prognosis of primary MN.

소실문자 판독에 관한 연구

양후열,김동욱,최섭,진명수,이영수,나기현 內務部 國立科學搜査硏究所 2002 國立 科學 搜査 硏究所 年報 Vol.34 No.-

제주도 북방, 함덕연안해역에 있어서 춘·하계 식물성 무각편모조류의 동태

윤양호,노홍길,김영기 제주대학교 해양연구소 1990 해양자원연구소연구보고 Vol.14 No.-

간세포암에 대한 화학 색전 및 주입 요법 후에 발생한 발열과 감염성 합병증에 관한 전향적 연구

김양수,이상오,이미숙,정두련,김은옥,정영화,우준희,이영상,서동진,성규보,류지소,고기영 대한감염학회 1998 감염 Vol.30 No.3

목적 : 간동맥 화학 색전 및 주입 요법을 시행 받은 환자에서 시술 후 나타나는 발열 및 감염성 합병증의 빈도와 특성을 관찰하고, 이들 중 감염성 합병증이 발생한 환자들을 미래 예측할 수 있는 임상적 지표들이 있는 지 알고자 하였다. 방법 : 1997년 6월부터 8월까지 간세포암으로 간동맥 화학 색전 및 주입 요법을 시행받은 환자들을 대상으로 전향적으로 조사하였다. 화학 색전 및 주입 요법 시행 후 발열이 발생하였으나 감염 및 간 회 색전증의 증거 없이 자연히 소실된 경우에 한해 색전술 후 증후군이라고 정의하였다. 결과 : 284명에서 303예의 시술(화학 색전 요법 200예, 주입 요법 103예)이 시행되었다. 이중 108예(35.6%)에서 시술 후 발열이 있었다. 발열의 원인으로 감염성 합병증이 7예 (2.3%), 허혈성 담낭염이 5예(1.7%)였고 1예는 분류가 힘들어 나머지 95예(31.4%)를 색전술 후 증후군으로 분류하였다. 감염성 합병증은 배양 음성 중성구성 복수(culture-negative neutrocytic ascites)가 3예, 원발성 세균성 복막염이 2예(이중 1예는 패혈증 동반), 패혈송 쇽이 1예, 요로 감염증이 1예이었다. 색전술 후 증후군은 발열이 없었던 군에 비해 복수가 있거나(odds ratio [OR], 2.74; 95% contidence interval [CI], 1.30∼5.75) 종양이 클수록 (OR, 1/15; 95% CI, 1.06∼1.24), 도한 좌우 감동맥을 모두 막은 경우 (OR, 1.81; 95% CI, 1.00∼3.28) 빈번하게 발생하였다. 감염성 합병증일 때에는 색전술 후 발열이 있으면서 systemic inflammatory response syndrome(SIRS)의 기준을 만족하거나 (p<0.05) 시술 후 72시간 이후에 복부 동통이 발생하는 경우가 많았다.(p<0.01). 그러나 발열 시 감염을 미리 구별할 수 있는 검사실 소견은 없었다. 결론 : 간세포암에 대한 화학 색전 및 주입 요법 후 색전술 후 증후군은 31.4%, 감염성 합병증은 2.3%에서 발생하였다. 색전술 후 발열이 있으면서 SIRS의 기준을 만족하거나 시술 후 72시간 이 후에 복부 동통이 발생할 때에는 감염성 합병증을 반드시 고려해야 하겠다. Background : Transarterial chemoembolization(TACE) and chemoinfusion (TACI) are the methods used increasingly in hepatocelluar carcinoma patients. In this study, the incidence of fever and infectious complications after TACE or TACI in pateints with hepatocellular cacinoma was investigated and clinical parameters predicting such complications were evaluated. Methods : Prospective surveys were done in hepatocelluar carcinoma patients who had undergone TACE or TACI from June August, 1997. Three hundred three treatment cases with TACE (200) or TACI (103) in 284 patients were analyzed. The postembolization syndrome was defined as fever following TACE or TACI that spontaneously subsided without the evidence of infections or extrahepatic emboilizations. Results : One hundread eight (35.6%) out of 303 cases showed fever after the treatment. Regarding the etiology of the fever, after the treatment. Regarding the etiology of the fever, infections occurred in 7 cases (2.3%), and ischemic cholecystitis existed in 5 cases (1.7%). In one case the cause was not clear and 95 cases (31.4%) were classified as the postembolization syndrome. Out of the infections, three had culture-negative neutrocytic scites, two spontaneous bacterial peritonitis (one was accompanied with sepsis), one septic shock, and one urinary tract infection. The postembolization syndrome was more frequently found in the cases with ascites (odd ratio [OR], 2.74; 95% confidence interval[CI], 1.30∼5.75), with larger tumor (OR, 1.15; 95% CI, 1.06∼1.24) and with embolization both hepatic arteries (OR, 1.81; 95% CI, 1.00∼3.28). In the cases with infections, many satisfied the criteria for systemic inflammatory response syndrome (SIRS, P<0.05) or had abdominal pain 72 hours later (P<0.01) after the treatment. However, no other laboratory data predicted infection among the cases with fever. Conclusion : After TACE or TACI in hepatocellular carcinoma patients, 31.4% of cases showed the postembolization syndrome and 2.3% of cases showed infection. Infection should be considered as a cause of fever in patients satisfying the criteria for SIRS or with abdominal pain 72 hours later after the treatment.

대황황련해독탕의 사염화탄소 유발 간장해 보호효과 미치 급성독성

김영석,정은아,장종철,양형길,김남재,조기호,배형섭,이경섭,김동현 WHO COLLABORATING CENTRE FOR TRADITIONAL MEDICINE 2002 東西醫學硏究所 論文集 Vol.2002 No.-

ABSTRACT - This study was performed to evaluate hepatoprotective effect of daewhang-whangryunhaedok-Tang(DWT) on liver injured rats induced by CCI_4 and the acute oral toxicity of it in mice. The activities of serum transaminase(ALT/AST), alkaline phosphatase(ALP) and lactic dehydrogenase(LDH), the levels of serum total cholesterol(TC) and triglyceride(TG), change of liver enlargement, and inhibitory activities of lipid perotidation, catalase and glutathione-S-transfrease(GST) in liver microsome were determined in hepatotoxic rats induced by CCI_4. DWT was significantly reduced the serum ALT, AST, ALP, LDH. TC and TG lecels. And, the increase of lipid peroxidation, decrease of catalase and GST activities in the liver microsome of CCI_4-intoxicated rat were significantly improved by the treatment of DWT. Male and female mice were administered maximum dosages of 5.000 mg/kg b.w. of DWT. After single oral administration of DWT to mice, we observed them daily for 2 weeks.DWT did not induce any toxic signs in the mortalitie, clinical signs, body weight changes, and gross necropsy finfings of mice. Based in these results. It is concluded that DWT may have the hepatoprotective effect on CCI_4 induced hepatotoxicity in rats. Also. DWT may have no side effect and its LD_50 value may be over 5.000mg/kg b.w. in mice.

무선통신 시스템을 위한 새로운 랜덤 접속 방법

신영주,임민중,양기주 동국대학교 산업기술연구원 2009 산업기술논문집 Vol.18 No.2

유선통신 시스템에서는 TDMA 타입의 랜덤 접속 방법들이 많이 사용되지만 무선통신 시스템에서는 TDMA 타입의 랜덤 접속 방법들뿐만 아니라 충돌을 줄일 수 있는 CDMA 타입의 랜덤 접속방법들도 많이 사용된다. 만일 적절한 전력 제어가 이루어진다면 CDMA 타입의 랜덤 접속은 제한된 수의 사용자들이 랜덤 접속할 때 검출 확률을 증대시킬 수 있다. 그러나 만일 전력 제어가 정확히 되지 않는다면 CDMA 타입의 랜덤 접속 방법은 성능이 떨어지게 되고 TDMA 타입의 랜덤 접속보다 오히려 성능이 나빠질 수 있다. 이 논문에서는 CDMA 타입과 TDMA 타입의 랜덤 접속의 결합 방법인 부분적 확산 랜덤 접속 방법을 제안한다. 제안하는 방법은 정밀하지 않은 전력제어가 이루어지는 시스템에서 CDMA 타입의 랜덤 접속이나 TDMA 타입의 랜덤 접속보다 우수한 성능을 낼 수 있다. While TDMA-type random access techniques are popular in wired communication systems, CDMA-type random access techniques are used as well to reduce collisions for wireless communications. If proper power control is applied, CDMA-type random access can increase detection probabilities with limited number of access users. However, if power control is not precisely performed, the performance of CDMA-type random access is degraded and can be worse than that of TDMA-type random access. In this paper, we propose partial spreading random access, which is a combination of CDMA-type and TDMA-type random accesses. The proposed method can outperform both CDMA-type and TDMA-type random accesses for partially power-controlled systems.

수면단계 및 체위에 따른 수면무호흡의 빈도 및 정도에 관한 연구

윤인영,정도언,민양기 大韓神經精神醫學會 1995 신경정신의학 Vol.34 No.4

Among the factors determining severity and frequency of obstructive sleep apnea sysdrome(OSAS), sleeping position and sleep stages seem to be crucial ones, supported by patients' & spouses' reports and clinical observations. This study was done to elaborate on the possible differences in the frequency and the severity of OSAS between supine and lateral positions as well as between REM and NREM sleep stages. Fourteen patients with clinical diagnosis of OSAS were studied polysomnographically for one full night using Oxford Medilog SAC 847 system. Standardized procedures were adopted for sleep recording with the body position sensor placed on the chest at the midline. Obtained computer-scored data were manually corrected regarding sleepstages, apnea types and the presence of arousals. IN the lateral position, compared with the supine position, respiratory disturbance index(RDI), O₂ desaturation and the percent arousals all decreased and the mean of lowest O₂ saturations increased significantly. During REM sleep, in comparison with NREM sleep, RD duration was more prolonged with O₂ desaturation more pronounced. The severity and the frequency of respiratory disturbances are more marked in the supine position and REM stage than in the lateral position and NREM stage respectively. Sleeping position training should be encouraged as an adjunctive therapeutic option, in addition to the adoption of other therapeutic maneuvers.

대황황련해독탕의 항고지혈증 작용

김영석,정은아,장종철,양형길,김남재,조기호,배형섭,이경섭,김동현 경희대학교 동서의학연구소 2001 東西醫學硏究所 論文集 Vol.2001 No.-

Whangryunhaedok-Tang (WT) is formulated with Coptidis Rhizoma, Phellodendri Cortex, Scutellariae Radix and Gardeniae Fructus, and Daewhang-whangryunhaedok-Tang (DWT) is made by the combination of Rhei Rhizoma, a wellknown anticostipation drug in WT. Therefore, DWT has been evaluated for antihyperlipidemic effects on experimental hyperlipidemic rats and mice induced by corn oil and high cholesterol-diet. Oral administration of DWT significantly inhibited the increase of serum triglyceride and LDL-cholesterol levels, and the decrease of serum HDL-cholesterol levels in hyperlipidemic rats induced by corn oil. Also, oral administration of DWT significantly prevented the increase of serum total cholesterol, triglyceride and LDL-cholesterol, and liver total cholesterol and triglyceride in 1% cholesterol-diet fed mice. These results suggest that DWT is effective for the treatment of hyperlipidemia.

L - NAME 주입시 신 교감신경이 신혈류량 변화에 미치는 영향

민영기,양훈모,이석호,김종규 순천향의학연구소 2000 Journal of Soonchunhyang Medical Science Vol.6 No.2

Recently it is known renal medullar will be play a important role of blood pressure control and pressure natriuresis. Renomedullary antihypertensive mechanism is activated by increasing perfusion pressure of kidney and can be inhibited by sympathetic nerve stimulation or blockade of nitric oxide(NO) formation. It is important to know what is affect on renal regional blood flow, especially renal medullary blood flow(MBF). For the purpose of investigating the effect of the renal sympathetic nerve on regional blood flow during infusion of blocking NO synthesis(by N^(ω)-nitro-L-arginine methylester:L-NAME) in spontaneous hypertensive rat(SHR) and Wistar rats normal control, Mean arterial pressure(MAP), total renal blood flow(TRBF), midcortical blood flow(CBF), inner medullary blood flow were measured in innervated or denervated renal sympathetic nerve group of SHR and Wister rats. All group that received the L-NAME were seen increasing MAP while TRBF, CBF and MBF decreased and the decrease of TRBF in SHR is more marked than in Wistar rat. In spite of increasing the MAP, MBF in all four group decreased, respectively C: 220 ± 3 U, 205 ± 2 U Wi : 215 ± 4 U, 165 ± 12 U Wd :218 ± 3 U, 170 ± 10 U Si :195 ± 4 U, 98 ± 15 U Sd : 200 ± 3 U, 120 ± 14 U, especially the most remarkable decrease about 50 % in SHR innervated group. Thus, according to these result, NO seems to maintain the MBF and the partial interaction between renal sympathetic nerve and NO in medullary blood flow can be suggested in innervated SHR.