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흰쥐 중격측좌핵의 신경전달물질에 대한 뇌 해마 제거 영향
김영석,김종규 순천향의학연구소 2002 Journal of Soonchunhyang Medical Science Vol.8 No.2
A study was planned to analyze glutamate and aspartate in the nucleus accumbens septi of hippocampectomized rats, dorsal hippocampectomized rats and ventral hippocampectomized rats by high-performance liquid chromatography (HPLC). 40 male albino rats were divided into 5 groups. Under chloral hydrate anesthesia(20%, 2㎖/㎏ i.p.), a hole was made in each parietal bone about 4 ㎜ lateral to the sagital suture, and 4㎜ rostral to the lambda suture. The cortical tissue on each side was removed and through this opening, the hippocampal tissue anterodorsal to the hippocampal flexures on both sides was removed by aspiration. This preparation served as the dorsal hippocampal group. The cortical tissue on each side was removed and, through this opening, the hippocampal tissue posteroventral to the hippocampal flexures on both sides was removed by aspiration. This preparation served as the ventral hippocampal group. The cortical tissue on each side was removed and, through this opening, the hippocampal tissue as much as possible on both sides was removed by aspiration. This preparation served as the hippocampal group. The cortical control group received the same surgery short of hippocampectomy. The normal rats served as normal control group. One day later, the animals were sacrificed by decapitation in the cold room. two to three milligrams of tissue was obtained from the accumbens septi in one side of the brain. The tissue samples were homogenized in 200 ㎕ of 0.5 M perchloric acid in 1 mM EDTA with ground-glass homogenizers. After centrifugation at 3,500 rpm for 15 min, an aliquot of 80 ㎕ supernatant was neutralized with 20 ㎕ of 2 M KHCO_3, and then centrifuged again at 3,500 rpm for 10 min. An aliquot of 20 ㎕ was taken and diluted with 350 ㎕ of β-mercaptopropionic acid in 10 ml of 100 mM sodium borate buffer (PH 9.1) and 350 ㎕ of OPA solved in ethanol which was diluted with 10 ml of 100mM sodium borate buffer (PH 9.1). Then 200 ㎕ of the resulting sample was injected with auto injector. Peak areas were automatically integrated and calculated by chromatopac. In the nucleus accumbens septi, the contents of glutamate decreased significantly in the dorsal hippocampal group and hippocampal group compared to those in the normal contrl group (P<0.001) and the cortical contrl group (P<0.001). The contents of glutamate increased significantly in the ventral hippocampal group than in the normal cotrol group (P<0.001) and the cortical control group (P<0.001), while there were no significantly different between the 2 control groups. The contents of glutamate were significantly different between the ventral hippocalpal group, the dorsal hippocampal group and the hippocalpal group. The contents of glutamate were significantly different between the hippocampal group and the dorsal hippocampal group. But the contents of aspartate were not significantly different among the all groups. It is inferred from the above mentioned results that excitatory transmitter substances employ the glutamate and apartate in accumbens septi, and that the dorsal hippocampus was facilitatory to the accumbens septi and that the ventral hippocampus was inhibitory to the accumbenns septi, and that there was a functional differentiation whithin the hippocampus.
김영석,이문성 순천향의학연구소 2004 Journal of Soonchunhyang Medical Science Vol.10 No.2
Polymyositis is one of the idiopathic inflammatory myopathy, characterized by myalgia, progressive symmetrical proximal muscle weakness, chronic non-suppurative inflammation of striated skeletal muscle, elevation of muscle enzyme, and electromyographical change. The main pathogenesis is the cell mediated immunity, and some cases were associated with viral infection which developed autoimmunity. 59 years old female patient who did not have evidence of hepatitis B virus neither hepatitis C virus infection, was complaint of generalized muscle weakness and swallowing difficulty. The patient revealed characteristic findings of polymyositis on muscle biopsy with electromyographical changes. Also, liver biopsy specimen showed chronic hepatitis with mononuclear cell infiltration in widened portal tract and the lobular parenchyme. We experienced a case of polymyositis which involved liver and developed chronic hepatitis comfirmed by liver biopsy. So we report this case with a review of the literature.
Gibberellin처리가 Cyclamen의 生育 및 開化에 미치는 영향
金英奭 대구교육대학교 1969 論文集 Vol.5 No.-
The following conclusion was reached after comparing and testing the effects that Gibberellin treatment gave on the Cyclaman growth. First, In the growth of peduncle, compared with the standard area, the 1-5 ppm Gibberellin area had 1∼2.4cm longer one while the 10-15ppm Gibberellin area on the contrary 0.2∼2.2cm shorter one. The reason of the above result, I think, was that the thickness of the 1-5 ppm area was proper to the growth, while that of the 10-15 ppm area was very thick and harmful to the growth. Second, In the flowering, the size of the flower-petal in the area given Gibberellin treatment was ramarkably bigger than that of the standard area. I would say the reason was that on the same day when I measured the size of the maturest petal in the Gibberellin area, I measured the flower-bud in the standard area. It took 17-27 days for the bud to fully bloom, so I could guess that the time of blooming came earlier: the 5ppm area having the earliest blooming and the 10 ppm area the next. Third, In the leafstalk growth, all the areas given Gibberellin treatment had shorter leafstalks than those of the standard area. So I could realize that bad effects of chemicals worked on the leafstalk growth. Forth, In the leaf growth, all the areas given Gibberellin treatment had smaller leaves than the standard area because of some chemical's-harm. Particulary, of them, the 15 ppm area which was most seriously harmed by chemicals had the smallest leaves while the 1 ppm area had the biggest leaves, but smaller than those of the standard area. But the leaves of the 1 ppm area were smaller than those of standard area. From the above tests and observations, I can conclude as follows: If the thickness of Gibberellin treatment is proper, the peduncle grows longer and flowers begin to bloom 17∼27 days earlier. But because of chemical's harm, no effect was had on the growth of both leafstalks and leaves in the whole Gibberellin treated areas, regardless of thickness.