염산 독세핀 외용제의 피부 전달 시스템을 위한 제제 설계 및 평가

이은미,김정수,경기열,이계원,지웅길 忠南大學校 生命科學硏究院 醫藥品開發硏究所 2006 藥學論文集 Vol.21 No.-

Doxepin hydrochloride(Dox) is a dibenzoxepin-derivative tricyclic antidepressant and used topically for the short-term relief of moderately severe pruritus associated with atopy dermatitis. Dox topical emulsion(or liposome) formulations were evaluated with respect to their rheological properities, permeation through excised skin of hairless mouse and in vitro/in vivo accumulation in the skin. The apparent viscosity was increased according to the concentration of glycerin and decreased with an increase in the concentration of phosphatidylcholine(PC). The permeation rate of formulation increased with a high concentration of PC and showed no relationship with the change of formulations. Amount of accumulated drug in the excised skin of hairless mouse was decreased with a high concentration of glycerin, but increased according to PC concentration.

피부 적용 겔제제의 첨가제에 의한 수분 보유력 평가

이은미,박종희,경기열,전일순,조혜정,지웅길 충남대학교 약학대학 의약품개발연구소 2004 藥學論文集 Vol.19 No.-

The aim of this study was to improve the moisturizing and water retaining effects of a gel containing poloxamer 407, glycerin, sod hyaluronate, sorbo(sorbitol 70%). Topical preparations were formulated as a gel containing different concentration poloxamer 407, glycerin, sod. hyaluronate, sorbo(sorbito1 70%), lipid(ceramide. sphingosine ratio 1:l). We tested a various combination of different additives for the moisturizing effect The water holding capacity of the mixture was measured using a moisture analyzer. In vivo test was carried out on human skin using Skicon-200 and water retaining effect was increased by addition of lipid. Water evaporation test was performed by various concentration of additives and water evaporation characteristics of the preparations were determined using the moisture analyzer. Moisturizing and water retaining effects was increased by adequate combination of various additives with topical gel formulations.

농작물 해충 및 진균류 방제를 위한 방선균의 분리 및 동정

이은정,강경돈,황교열,김두호,김신덕,성수일 한국잠사학회 1998 한국잠사곤충학회지 Vol.40 No.1

Twenty seven out of ca. 5,000 actinomycete strains, which were isolated from soil collected throughout the country, showed antimicrobial effects against fungi, Rhizopus stronifer (ATCC6227a), Rhizoctonia solani (KCCM 11271) and yeast, Candida albicans(ATCC10231). From these antifungal microorganisms, we further selected seven strains which seemed to produce insecticidal substances with in vivo test, using silkworm, Bombyx mori and beet armyworm, Spodoptera exigua. Morphological and biochemical experiments revealed that three strains out of seven were streptomyces. Further investigations on the physical and chemical properties of these antifungal and insecticidal substances are now in progress.

석면관련 폐질환의 진단에 있어서 흉부고해상도전산화단층촬영검사(HRCT)의 유용성

이현재,손지언,,홍영습,,이영일,예병진,유창훈,정갑열,김상훈,임형준,장은철,김정만,김준연 大韓産業醫學會 2006 대한직업환경의학회지 Vol.18 No.2

목적: 석면에 노출된 근로자에서 석면관련 폐질환의 양상을 분석하고 진단에 있어 HRCT의 유용성을 연구하여 석면에 대한 의학적 감시프로그램의 개발에 기여하고자 하였다. 방법: 석면에 직접 노출되는 작업자 68명과 간접 노출되는 작업자 94명 등 총 162명에 대해 OSHA 석면 표준, 의학적 감시 프로그램에 따라 단순 흉부방사선검사, 폐활량검사 및 우리나라 실정에 맞게 일부 수정한 의학적 설문지를 통해 면접조사의 방식으로 환자에 관한 정보를 수집하였으며, 자료의 신뢰성을 확보하기 위하여 면접조사는 1달 이상의 기간을 두고 2차례에 걸쳐 실시된 후 확인, 수정하였다. 또한, 석면관련 폐질환을 확진하기 위하여 HRCT를 추가적으로 실시하였다. 결과: HRCT결과 석면관련 폐질환의 유병율은 162명의 근로자중 17명으로 10.5%였는데, 직접 노출군에서 10명 (14.7%), 간접 노출군에서 7명 (7.4%)이었다. HRCT와 비교할 때 단순 흉부방사선검사의 민감도와 특이도는 각각 70.6%와 98.6%이었고, 양성 예측도와 음성 예측도는 각각 85.7%와 96.6%으로, HRCT는 단순흉부방사선검사에 비해 민감도가 높아 초기 석면관련 폐질환을 진단하는데 효과적이었다. 석면관련 폐질환의 진단에 있어 HRCT와 폐활량검사 사이에 유의한 관련성이 관찰되었다. HRCT 결과 석면관련 폐질환의 유무와 의학적 설문조사 결과의 빈도분석을 실시한 결과, 유의한 변수는 작업기간, 흡연량, 호흡기질환의 과거력, 기침, 호흡곤란이었다. 결론: HRCT는 석면관련 폐질환의 진단에 있어 단순 흉부방사선검사와 비교하여 민감도가 높아 초기 진단에 유용하므로 단순 흉부방사선선검사 결과 석면관련 폐질환소견을 보일 때뿐만 아니라, 정상소견을 보이더라도 폐활량 검사결과와 근무력, 흡연력, 호흡기질환의 과거력, 기침, 호흡곤란 등의 호흡기 증상을 참고하여 HRCT검사를 고려해야 한다. 또한, 본 연구에서 관련성이 없었던 요인들에 대해서도 향후 연관성을 규명하는 광범위한 전향적 연구가 필요하다. Objectives: This study was carried out to improve the medical surveillance program of workers exposed to asbestos by examining the usefulness of High Resolution Computed Tomography (HRCT) in the diagnosis of asbestos-related lung disease. Methods: The study subjects comprised 162 workers in a ship-repairing yard, 68 of whom had been directly exposed to asbestos and 94 indirectly exposed. The 'Occupational Safety & Health Administration (OSHA) asbestos standard, medical surveilance program' and HRCT were conducted to analyze the aspects of the asbestos-related lung disease. The OSHA asbestos standard, medical surveilance program consists of simple chest x-ray, spirometry and medical questionnarie. Results: Seventeen (10.5%) of the 162 subjects, 10 (14.7%) directly exposed and 7 (7.4%) indirectly exposed, revealed asbestos-related lung disease on HRCT. The sensitivity and specificity of simple chest x-ray for asbestos-related lung disease were 70.6% and 98.6%, and the positive and negative predictive values were 85.7% and 96.6% respectively, as compared with HRCT, HRCT was an effective diagnostic tool especially to detect early asbestos-related lung disease. The study results indicated a relative significant association between the results of spirometry and HRCT. The variables significantly associated with asbestos-related lung disease on HRCT were work duration, smoking history (pack-years), past history of respiratory disease, cough and dyspnea. Conclusions: In the diagnosis of asbestos-related lung disease, HRCT should be considered not only for workers with positive findings on simple chest x-ray, but also workers with specific findings on spirometry, occupational history, smoking history, and past history of respiratory disease, or with respiratory symptoms such as cough and dyspnea.

인간 타액선세포 및 선암종세포주의 분화 유도시 Transglutaminase발현과 고정의존성 성장과의 관계

박대열,이선경,이화정,장현주,민승기,김은철 大韓顎顔面成形再建外科學會 2003 Maxillofacial Plastic Reconstructive Surgery Vol.25 No.2

Transglutaminase(TGase) catalyzes the Ca^2+ dependent acyl transfer reaction to from γ-glutamyl lysine cross-links between substrate proteins. we have examined the expression of TGase 1 and TGase 2 enzyme activity and protein expression in differentiation induced salivary gland cells(HSG), and human salivary adenocarcinoma cell lines(SGT)in reation to anchorage independent growth. The TGase1 and TGase 2 activity in SGT was higher than in HSG, and there is no significant difference between TGase 2 and TGase1 cells. HSG cells showed no increase in TGase 1 and TGase 2 upon reaching post confluence and in 1.8mM Ca^2+. Expression of the TGase 1 and TGase 2 was induced by high cell density or by high calcium in SGT. No induction of TGase 1 & 2 protein was observed in postconfiuent HSG cells, but there was notable increases in TGase 1 & 2 protein in post confiuent SGT cells. This pattern of TGase 1 & 2 protein expression correlates with the pattern of particulate activity. SGT cells showed more colony size and colony forming activity in soft agar rather than HSG cells. The ability of SGT cells, containing high levels of TGase 2 and TGase1,to form colonies in soft agar suggests a role for this enzyme in anchorage independence.

배추 시스테인 단백질 분해 효소 억제 유전자 BCPI-1의 발현과 종자 발아 및 유묘 생장과의 연관성

홍준기,이은영,김정률,양경애,최영주,정우식,김호일,윤대진,이상열,조무제,임체오 Plant molecular biology and biotechnology research 2003 Plant molecular biology and biotechnology research Vol.2003 No.-

Phytocystatins are protein inhibitors of cysteine proteinases of the papain family that have been identified in both monocot and dicot plants. A cDNA encoding a phytocystatin, BCPI-1 (Brassica Cysteine Proteinase Inhibitor-1) has been isolated from Chinese cabbage (Brassica campestris L. ssp. pekinensis) flower buds. Here, we tested whether BCPI-1 transcription is regulated by hormones, and could be involved in regulating cysteine proteinases during and after germination. BCPI-1 was sensitive to exogenous GA_(3) and ABA, which are important factors controlling seed germination, suggesting that the expression of BCPI-1 is hormonally regulated. We introduced a recombinant plasmid containing the full-length BCPI-1 cDNA under the control of cauliflower mosaic virus 35S promoter into rice embryogenic calli using the particle delivery method, and regenerated a number of transgenic rice plants. Constitutively over-expressed BCPI-1 caused changes in overall plant growth and development, including reduced germination and seedling growth. These data support the role of the BCPI-1 in the regulation of endogenous proteinases during both seed germination and subsequent seedling development. Phylocystatins은 papain계열의 cysteine 단백질 분해 효소 활성을 특이적으로 억제하는 억제자로 다양한 식물 종으로부터 분리되었다. 본고에서는, 배추 화아 cDNA library로부터 분리된 phytocystatin인 BCPI-1(Brassica Cysteine Proteinase Inhibitor-1)의 생체 내 기능에 대해 연구하였다. 먼저, BCPI-1 전사체는 발아 조절에 중요한 영향을 미치는 GA₃와 ABA에 의해 예민하게 증가, 혹은 감소되는 반응을 보임으로써, BCPI-1이 식물 호르몬의 영향을 받으며, 특히 발아나 유묘의 생장 조절에 관련이 있음을 알 수 있었다. Particle bombardment 방법을 통하여 BCPI-1을 벼의 배 형성 세포 내에 도입, 재분화 시켜 형질 전환 벼를 생성하였다. CaMV 35S promoter의 조절에 의해 지속적인 BCPI-1 발현을 보이는 형질 전환 벼의 경우, 발아와 유묘 생장이 현저히 지연되었다. 위의 결과들을 바탕으로, BCPI-1이 식물체 내에서 생성되는 cysteine 계열의 단밸질 분해 효소 활성을 조절하여 종자의 발아와 유묘의 생장에 영향을 미치는 것으로 추측한다.

우리나라 파프리카 수출단지 및 선과장의 병해충 조사

김기돈,이시원,강은하,신용길,전재용,허노열,이흥식 충남대학교 농업과학연구소 2013 농업과학연구 Vol.40 No.2

The disease and insect were surveyed locally in greenhouse, fruit packing house and store house of 51 farms in 13 towns having purpose of paprika exportation. By analysis, various disease and insect were not only founded locally but more ones detected in farms having old facilities and no natural enemy. We found 15 pathogens such as Fusarium spp., Alternaria solani, Leveilluila taurica, PepMV (Pepino mosaic virus) and TMV (Tobacco mosaic virus) in greenhouse, Fusarium spp. in fruit packing house and Penicillium spp. in store house. We found 15 insects in greenhouse such as Bemisia tabaci, rialeurodes vaporariorum and Myzus persicae in greenhouse, Hylobitelus haroldi in fruit packing house. However, the problem quarantine disease and insect for importation and exportation were not detected in inspection time.

점회절 광간섭계를 이용한 연마된 실리콘 웨이퍼 표면형상 측정

권택민,김승우,김학용,이은상,이상열 한국공작기계학회 2008 한국공작기계학회 춘계학술대회논문집 Vol.2008 No.-

We devised a vibration-insensitive interferometer with a common-path scheme of generating the reference wave directly by transmitting the measurement wave through a single-mode fiber. This fiber-diffraction interferometer enables to cancel out the dominating vibration effect encountered commonly in both the reference and measurement waves in the due course of the two waves' interference. Besides, to suppresss parasite vibration disturbances caused to interferometer hardware components, a special type of spatial phase-shifter is added to capture four phase-shifter interferograms simultaneously without time delay using a single camera shot.

임실관촌 사선대 일대의 식물상과 분포

길봉섭,김창환,김무열,이은복 원광대학교 생명공학연구소 1997 생명공학연구소보 Vol.5 No.1

임실 관촌 사선대의 관속식물은 66과 145속 177종 1아종 20변종 4품종으로 총 202종류가 분포하고 있는 것으로 조사되었다. 조사기간이 가을 한 철에 불과했던 사정을 감안한다면 이보다 더 많은 종류가 있을 것이 확실하다. 사선대의 식물군락은 식물사회학적인 방법으로 분류한 결과 목본은 졸참나무군락(Quercus serrata community), 가침박달군락(Exochorda serratifolia community), 개서어나무군락(Carpinus tschonoskii community), 느티나무군락(Zelkova sarrata community), 상수리나무군락(Quercus acutissima community), 갈참나무군락(Quercus aliena community), 긴산개나리군락(Forsythia saxatilis var. lanceolata community) 그리고 리기다소나무식재림(Pinus rigida plantation) 등으로 구분되었고, 초본은 복수초군락(Adonis amurensis community), 피나물군락(Hylomecon vernale community)으로 총 9개군락과 1식재림으로 나누어졌다. 조사지의 식생은 피나물의 대형군락과 복수초군락, 긴산개나리군락 및 가침박달군락 등이 다른 곳에서 찾아보기 힘들 만큼 식물 분포학상 특이하며 그만큼 중요하므로 이를 특별히 보호해야 할 필요가 있다. 아울러 할미밀망, 큰잎산꿩의다리, 조팝나무는 한국특산식물이다. 흥미로운 점은 조사지가 비교적 낮은 곳에 위치하는데도 불구하고 할미밀망, 큰잎산꿩의다리, 피나물, 복수초 등 비교적 높은 곳에 분포하는 식물들이 이곳에 난다는 것은 국지적인 기후의 특수성 때문일 것으로 생각된다. 즉, 관촌 사선대 일대는 기온이 낮아서 옛부터 해빙이 늦게 되는 곳이라는 점이다. 또, 긴산개나리군락은 우리나라에서 가장 큰 자연군락이며 이곳이 분포의 남한계선이어서 이 군락지는 천연기념물로 지정되어 보호하고 있다. The flora of Saseon-dae, kwanchon, Imsil-gun was composed of 66 families, 145 genera, 177 species, 1 subspecies, 20 varieties, 4 forma and 202 taxa. The vegetaion was classified into 9 communities and 1 planted forest : Quercus serrata community, Exochorda serratifolia community, Carpinus tschonoskii community, Zelkova serrata community, Quercus acutissima community, Quercus aliena community, Forsythia saxatilis var. lanceolata community, Adonis amurensis community, Hylomecon vernale community and Pinus rigida plantation. Several ferns, for example, Pyrrosia linearifolia growing on the rock, Polystichum tsussimense, Camptosorus sibiricus, Asplenium trichomanes, Dryopteris lacera, and Dryopteris bissetiana, endemic species such as Clematis trichotoma, Thalictrum punctatum and Spiraea prunifolia var. simpliciflora were of great significance in terms of the geographical distribution of plants. Especial F. saxatilis var. lanceolata species are forming the biggest natural community and recognized southern boundary line of distribution in Korea, therefore, it should be protected with a special counterplan including designate as a precious natural monument.

심실 조기 수축의 발생 부위 및 연결 간격의 차이에 따른 혈역학적 변화

진승원,김재형,노태호,조은주,김희열,이만영,김종진,박준철,채장성,홍순조,최규보 대한순환기학회 1999 Korean Circulation Journal Vol.29 No.7