Choline dehydrogenase interacts with SQSTM1/p62 to recruit LC3 and stimulate mitophagy.

Park, Sungwoo,Choi, Seon-Guk,Yoo, Seung-Min,Son, Jin H,Jung, Yong-Keun Landes Bioscience 2014 AUTOPHAGY Vol.10 No.11

<P>CHDH (choline dehydrogenase) is an enzyme catalyzing the dehydrogenation of choline to betaine aldehyde in mitochondria. Apart from this well-known activity, we report here a pivotal role of CHDH in mitophagy. Knockdown of CHDH expression impairs CCCP-induced mitophagy and PARK2/parkin-mediated clearance of mitochondria in mammalian cells, including HeLa cells and SN4741 dopaminergic neuronal cells. Conversely, overexpression of CHDH accelerates PARK2-mediated mitophagy. CHDH is found on both the outer and inner membranes of mitochondria in resting cells. Interestingly, upon induction of mitophagy, CHDH accumulates on the outer membrane in a mitochondrial potential-dependent manner. We found that CHDH is not a substrate of PARK2 but interacts with SQSTM1 independently of PARK2 to recruit SQSTM1 into depolarized mitochondria. The FB1 domain of CHDH is exposed to the cytosol and is required for the interaction with SQSTM1, and overexpression of the FB1 domain only in cytosol reduces CCCP-induced mitochondrial degradation via competitive interaction with SQSTM1. In addition, CHDH, but not the CHDH FB1 deletion mutant, forms a ternary protein complex with SQSTM1 and MAP1LC3 (LC3), leading to loading of LC3 onto the damaged mitochondria via SQSTM1. Further, CHDH is crucial to the mitophagy induced by MPP+ in SN4741 cells. Overall, our results suggest that CHDH is required for PARK2-mediated mitophagy for the recruitment of SQSTM1 and LC3 onto the mitochondria for cargo recognition.</P>

파트너십에 의한 지하철 입구 공간의 쌈지공원 설계 및 시공

김승환,강현우,손용욱,박승범,남정칠,이기철,차민준 東亞大學校 建設技術硏究所 2009 硏究論文集 Vol.32 No.2

A pocket park is valued in securing green space in downtown and improving urban view in the aspect of natural and environmental values, and it signifies a lot as the center of local residents’ community and harmony as well. Since subway exits and surroundings that have high portions in taking up street space due to lack of green space in downtown aren't made full use of, valuable pocket park place. Dangni Pocket Park of subway exits is a park completed with cooperative works and partnership by students, specialists, administrative organizations, businesses and civilian organizations, etc. without support of special budget. The study site was finally chosen to the space between exit No.1 of Dangni subway station, after analyzing the surrounding circumstances, availability and accessibility of the space among the several subway station exits of Saha, Dangni, and Hadan Station in Busan Metropolitan city. For the designing process, The Citizens organization for Million Amenity Park performed the planning and project proceeding, and 19 design drafts were proposed during the class of landscape architecture in Dong-a University. Design review panel members discussed to select the best draft in practical and authentical, and the related experts reviewed and revised to present the final design plan. The construction was conducted was performed under the cooperation between the civil, authorities, and industrial organizations, among which Saha District office managed for the waste treatment and maintenance matters. The graduates of landscape architecture department of Dong-a University and related industrial enterprises supplied landscape materials and related labors, were supported by 20 members of GPM, one of clubs of Dong-a University volunteered durig the most of construction progress, completing the Pocket Park of subway exits through partnership.

대중교통 환승주차장의 CPTED 체크리스트에 의한 환경디자인 연구

김대용(Kim, Dae Yong),김태용(Kim, Tae Yong),정승호(Chung, Seung Ho),박현호(Park, Hyeon Ho) 한국전시산업융합연구원 2012 한국과학예술융합학회 Vol.10 No.-

본 연구는 늘어나는 대중교통인접 환승주차장의 범죄위험성으로부터 이용자들의 안전을 위하여 ‘환경설계를 통한 범죄예방(Crime Prevention Through Environment Design: CPTED, 이하: CPTED)"개념을 적용하여, 환경디자인 개선사항을 도출하는 연구이다. 연구의 방법과 범위로는 CPTED이론, 문헌분석, 관련법 및 법규, 관련 전문가그룹을 통하여 1차 정량·정성체크리스트를 설계하여 현장답사 하였으며, 이를 보안하는 2차 정성체크리스트를 설계, 현장답사 하였으며, 현장답사는 대중교통환승 주차장의 유형과 환승형태, 주차장규모, 운영주체등을 고려하여 수도권의 5개 환승주차장을 선정하였다. 설계된 체크리스트를 바탕으로 현장답사 결과 범죄위험성으로부터 환경디자인 개선사항은 5곳 중 1곳을 제외하고 4곳 모두 개선이 필요한 것으로 분석되었다. 분석은 주차지역, 유지관리, 전자보안, 안내표지, 조경, 조명으로 구분하였으며 조사대상지 대부분 범죄위험성으로부터 전자보안에만 의지하는 행태를 가졌으며, 그 또한 요금 징수 등을 주 목적으로 하고 있다. This study applies the concept of Crime Prevention Through Environmental Design (CPTED) and suggests ways to make improvements in environmental design to provide secure facilities for those who use park-and-ride structures which are increasing on a regular basis. First, the authors made a checklist for field examination after consulting related literature and related laws and holding discussion sessions with the experts of the field. After the field investigation with the quantitative and qualitative checklist, another qualitative checklist was developed to supplement the first one. With the second checklist, the authors visited five different park-and-ride structures around the city of Seoul that were diverse in layout, scale, and management system. The result showed that four out of the five sites assessed needed improvements. The analysis was divided into the following areas: location of the structure, maintenance, electronic security, signs, landscaping, and lighting. A popular method of crime prevention at the parking structures was electronic security which was in fact operated mainly to encourage the drivers to pay their parking fees.

당뇨병환자에서 뇨증 Fibronectin농도의 증가에 관한 연구

한승범,조준승,손건영,서성문,박근용,조성래,박규영,박정모,이인규,여준기 啓明大學校 醫科大學 1994 계명의대학술지 Vol.13 No.4

Plasma fibronectin is an α₂-glyoprotein, which is produced by vascular endothelial cells. Raised level of plasma fibronectin has been observed in diabetic patients particularly in the presence of microvascular complications. However, no available data exist about urine level of this glycoprotein in diabetic patients. We measured urine fibronectin level by ELISA methods in 54 diabetic patients who have microvascular comlications or not. The following results were obtained. 1) Urine fibronectin level(㎍/g creatinine) in diabetic group(1740.0±678.0) is increased compaired with those in normal control group(471.0±59.0). but this results were not significantly different among two groups(P>0.05). 2) Urine fibronectin level(㎍/g creatinine) was significantly increased in patients with nephropathy (6188.0±3144.0) compared with those in normal control group(471.0±59.0) and patients without nephropathy(645.0±251.0)(P<0.001). 3) There were a significant correlation between BUN, creatinine, creatinine clearance, 24hr urine total protein and urine fibronectin level in diabetic patients. Our data suggest that urine fibronectin excretion level might be used as a sensitive guide for diabectic nephropathy.

우심방을 침범한 거대 비호지킨씨 림프종 1예

강상범,진승원,이은경,박용현,최용호,김용주,박준철,김재형,홍순조,최규보,권종범,원용순,박건,이은희 대한순환기학회 2000 Korean Circulation Journal Vol.30 No.4

Reference Map of Soluble Protenis from Salmonella enterica Serovar Enteritidis by Two-Dimensional Electrophoresis

김곤섭,Eung-goo Lee,Yong-hwan Kim,정태성,Yong-seung Shin,Gee-wook Shin,Hui-guen Cha,Mi-rim Park 대한수의학회 2003 Journal of Veterinary Science Vol.4 No.2

Map of Soluble Proteins from Salmonella enterica Serovar Enteritidis byTwo-Dimensional ElectrophoresisMi-rim Park, Eung-goo Lee, Yong-hwan Kim, Tae-sung Jung, Yong-seung Shin, Gee-wook Shin, Hui-guen Cha and Gon-sup Kim*Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University, Jinju 660-701, KoreaReceived May 17, 2003 / Accepted July 23, 2003J. Vet. Sci. (2003), 4(2), 143-149JOURNAL OFVeterinaryScience*Corresponding author: Gon-sup Kim Laboratory of Veterinary Biochemistry, Collage of Veterinary Medicine, Gyeongsang National University, 900 Gazwa, Jinju 660-701 Korea Tel: +82-55-751-5823, Fax: +82-55-751-5803 E-mail: gonskim@nongae.gsnu.ac.kr

한의진단명과 진단요건의 표준화 연구 III : 3차년도 연구결과 보고

최선미,양기상,최승훈,박경모,박종현,심범상,김성우,노석선,이인선,정진홍,이진용,김달래,임형호,김윤범,박성식,송태원,김종우,이승기,최윤정,신순식 한국한의학연구원 1997 한국한의학연구원논문집 Vol.3 No.1

The diagnostic requirements were suggested and explained regarding the systems of differentiation of symptoms and signs in the third year study of standardization and unification of the terms and conditions used for diagnosis in oriental medicine. The systems were as follows : - analyzing and differentiating of epidemic febrile disease - analyzing and differentiating in accordance with the Sasang constitution medicine based on four-type recognition - differentiation of disease according to pathological changes of Chong and Ren channels - standards for diagnosis of women's disease - standards for diagnosis of children's disease - standards for diagnosis of motor and sensor disturbance (-muscle. born, joint, etc.) - standards for diagnosis of neuropsychiatric disease - standards for diagnosis of five sense organ disease - standards for diagnosis of external disease The indivisual diagnosis pattern was arranged by the diagnostic requirements in the following order : another name, notion of diagnosis pattern, index of differentiation of symptoms and signs, the main point of diagnosis, analysis of diagnosis pattern, discrimination of diagnosis pattern, prognosis, a way of curing a disease, prescription, herbs in common use, disease appearing the diagnosis pattern, documents. The standards for diagnosis of each disease was arranged by the diagnostic requirements in the following order : another name, notion of disease, the main point of diagnosis, analyzing and differentiating of disease, analysis of disease, discrimination of disease, prognosis, a way of curing and prescription of disease, disease in western medicine appearing the disease in oriental medicine, documents.

NaNO₂의 N 핵사중극 공명 연구

박영민,송승기,강재필,서용문 명지대학교 자연과학연구소 1995 자연과학논문집 Vol.12 No.-

펄스형 핵사중극 공명장치(Pulsed system)를 이용하여 상온에서 NaNO? 분말시료의 위와 아래 공명진동수, 핵사중극 결합상수, 비대칭인자와 스핀-스핀완화시간을 구하였다. FID공명선으로부터 구한 값은 υ+=4.6354 NHz/ υ-=3.5983 MHz, e?qQ/h=5.4891 MHz와n=0.3779이었다. 90?,ι, 180? 펄스연속(spin-echo 신호)과 r를 변화시켜 얻은 진폭을 이용하여 스핀 -스핀 완화시간 T2를 측정하였다. 이결과 υ(T?)=2.50ms, υ-(T?)=2.17ms임을 보였다. Measurements of the upper and lower resonance freqency, quadrupole coupling constant, asymmetry parameter and spin-spin relaxation time are reported for NaNO? powder sample by using the pulsed NQR system at room temperature.From FID line-shapes, we obtained υ=4.6354 MHz, υ=3.5983 MHz, e?qQ/h=5.4891 MHz and η=0.3779. The spin-spin relaxation time T? was maesured using a 90?,ι, 180? pulsed sequanco (spin-echo signals) and varying τwhile measuring the amplitude of the echo. The result showed υ+(T?) =2.50 ms and υ+(T?) =2.17ms.

정신분열병과 Neuregulin 1 유전자의 연합연구

박동연,이유상,조은영,조승희,장용이,전현옥,장수연,윤세창,김종원,홍경수 大韓神經精神醫學會 2005 신경정신의학 Vol.44 No.3

Objectives : The authors recently found a suggestive evidence of linkage of chromosome 8p21-12 to schizophrenia in Korean multiplex families. Neuregutin 1 (NRGI) was identified in this locus as a positional and functional candidate gene for Schizo-phrenia, through several independent studies with European and Chinese populations. The purpose of this study is to determine whether NRGl is associated with schizophrenia in Korean population. Methods : Three SNPs (SNP8NRG221533, SNP8NRG241930, SNP8NRG243177) and two microsatellites markers (478B14-848,420M9-1395) located at the 5' end of NRGI were genotyped for 242 unrelated schizophrenia patients and the same num-ber of normal controls. Genetic association was tested by χ²-test (df=1). Not only for the whole patients group but also for asubgroup of patients with auditory hallucination. This subtype showed stronger linkage with chromosome 8p12 in the prior study of the authors with multiplex families. Results : G allele of SNP8NRG241930 was significantly in excess in the subgroup of patients with auditory hallucination compared to the control group (p=0.03, 0R=1.76). We also found that 3 SNPs haplotype TTC (p=0.04, 0R=0.58) and five markers haplotype TTC53 (p=0.01,0R=0.49) were associated with schziophrenia with a protective effect. Three SNPs haplotype CGT which is a part of the at-risk haplotype of the Icelandic schizophrenia families was found in excess in the patients group but no significant association was observed. Conclusion : NRGI might either play a mle in the predisposition to schizophrenia or be in linkage disequilibrium with a causal locus of this illness.

여대생들의 SNS와 소통 : 서울여자대학교 재학생의 Twitter 및 Facebook 활용을 중심으로

박미현,전유리,김유솔,신윤지,노승용 서울여자대학교 2010 여성연구논총 Vol.25 No.-

This study focuses on the characteristics of communication by such social network service (SNS) as Twitter and Facebook. In order to achieve the goal of this study, interview to students in Seoul Women's University was introduced to figure out such communicative behavior using SNS as frequency, motivation, purposes, and effects of SNS. The interview results show that students in Seoul Women's University have strong desire to communicate each other and SNS is one of digital mechanism to enhance their communication. Also, the results point out that interviewees communicative behavior using SNS can be categorized into two types of communication, network- oriented communication to promote friendship and fact-oriented communication to collect and share information. Moreover, interviewees indicate that SNS has both positive and negative (for example, privacy and security, time-consuming, false information, and addiction) aspects, as a results, users' self-control and digital competency should be improved. 소통은 인간 사회의 핵심 가운데 하나이다. 인간은 다른 사람들과 소통하고 싶어 한다. 즉, 상대방에 대해 알고 싶어 하는 동시에 자신을 알리고 싶어 하는 욕구를 가지고 있다. 인간의 소통에 대한 욕구를 충족시키는 방법은 시대의 발전에 따라 변화해왔다. 수천년 전부터 인류는 직접 만나서 언어로서 서로의 감정을 표현하고 정보를 교환하며 지내왔다. 즉, 이 시기에는 소통에서의 시간적․공간적 제약이 존재하였다. 그러나, 문자와 종이가 발명되면서 인류는 소통에서의 시간적․공간적 제약을 극복하기 시작하였다. 인류의 소통은 전화의 발명으로 한발 더 나아가게 되었으며, 디지털 혁명은 인간에게 시간적․공간적 제약을 뛰어넘는 소통을 가능하게 해 주었다. Bill Gates가 “PC는 인간이 만든 도구 중 인간에게 가장 많은 권한을 부여하는 도구라고 자신있게 말할 수 있다. PC는 소통의 도구, 창의력의 도구이며 사용자가 직접 만들어 나갈 수 있다(I think it's fair to say that personal computers have become the most empowering tool we've ever created. They're tools of communication, they're tools of creativity, and they can be shaped by their user.)”라고 주장한 바와 같이, PC와 인터넷의 급속한 발전으로 시발된 디지털 혁명은 인간의 소통에도 20세기 후반에는 상상도 할 수 없었던 큰 변화를 가져왔다. 소위 제 3의 물결(Toeffler, 1980)이라 불리는 정보화 시대의 물결을 타고 현대인들의 소통에 대한 욕구를 더욱 더 편리하게 발현 할 수 있도록 하는 수단의 변화를 가져왔다. 현 시대의 사람들이 행하는 소통의 행태는 전통적인 소통의 방법과 디지털 네트워크의 소통방법이 함께 어우러져 행해지는 모습을 보이고 있고, 이와 더불어 인터넷 등을 통한 가상 커뮤니케이션의 방법으로도 이루어지고 있다. 그리고 디지털 네트워크 기술 발전에 따른 해당 시스템의 이용자가 증가하고 있으며, 관련 시스템이나 모바일 어플리케이션도 지속적으로 개발되어 유통되고 있다. 이러한 디지털 네트워크 발전 속에서 Social Network Service(이하 SNS)가 새롭게 등장했다. 정보기기와 해당 시스템의 발전으로, 면대면 형식의 소통을 벗어나 SNS를 통한 온라인상의 소통이 가능하게 되었고, 이는 다른 소통의 방법보다 상대적으로 편리함을 제공하여 SNS 이용자들에게 폭발적인 반응을 불러일으켰다. 2010년 초 미국에서 처음 쓰이기 시작한 신조어로 ‘TGIF’가 있다. ‘TGIF’는 트위터, 구글, 아이폰, 페이스북 등 스마트 열풍의 진원지라 할 수 있는 네 가지 서비스를 통칭하는 표현이다. TGIF가 급속히 확산된 지난 2년간 세계인들은 정치․경제․사회․문화 모든 면에서 큰 변화를 겪었다. 2008년 당선된 미국의 오바마 대통령은 세계 최초의 트위터 대통령이라 할만하며, 2011년 초에 발생한 튀니지의 ‘재스민 혁명’과 이집트의 ‘키파야 혁명’ 또한 ‘TGIF’의 소산이었다. 오바마 대통령의 당선 이후 유튜브 정치(YouTube Politics)란 용어가 탄생하였고, 2011년 1월 26일 미국 오바마 대통령의 연두교서는 유튜브를 통해 세계에 생중계 되었다. 이러한 정치 분야에서의 소통 뿐만 아니라 기업이 직접 SNS계정을 만들어 네트워크상에서 자신의 고객과의 소통을 하는 기업이라는 조직 대 개인의 소통도 증가하고 있다. KT나 삼성 등의 대기업 등은 SNS를 이용한 고객과의 소통을 통해 기업이미지 제고와 고객과의 친밀함을 시도하고 있는 것은 이미 주위에서 많이 볼 수 있다.