RT-LAMP를 이용한 콩황화일반모자이크바이러스의 진단

배대현, 이영훈, 김봉섭, 윤영남, 강범규, 최만수, 구성철, 김현태, 윤홍태, 이수헌, 백인열 忠北大學校 農業科學硏究所 2014 農業科學硏究 Vol.30 No.2

Soybean yellow common mosaic virus (SYCMV) has been recently reported, it has been occurred a lot with Soybean mosaic virus (SMV) and Soybean yellow mottle mosaic virus (SYMMV) in soybean field. SYCMV belongs to genus of Sobemovirus and induced viral symptoms with yellowing, mottle and mosaic. A reverse transcription loop-mediated isothermal amplification (RT-LAMP) method allowed one-step detection of gene amplification by simple procedure and needed only a simple incubator for isothermal template. This RT-LAMP method allowed direct detection of RNA from virus-infected plants without thermal cycling and gel electrophoresis. In this study, we designed RT-LAMP primers named SYCML-F3/B3/FIP/BIP from coat protein gene sequence of SYCMV. After the reaction of RTLAMP, products were identified by electrophoresis and with the detective fluorescent dye, SYBR Green I. under daylight and UV light. Optimal reaction condition was at 63 for 60min and the primers of RTLAMP showed the specificity for only SYCMV tested in this study.

혈액 투석 환자에서 중심정맥 협착에 대한 스텐트 삽입술 : Wallstent Placement

임대승,노상필,이유선,정승현,김보영,이정우,강정아,김정희,이민수,정준용,최시완,정진옥,성인환,이강욱,신영태 충남대학교 의과대학 의학연구소 2002 충남의대잡지 Vol.29 No.1

Stenosis of central vein is a common complication arising after percutaneous subclavian vein catheter insertion performed for temporary vascular access in chronic renal failure patients undergoing hemodialysis. There are several treatment methods for the condition like percutaneous angioplasty(PTA), stent insertion, and surgery, but recent trend is toward PTA and stents. Among the patients diagnosed with chronic renal failure from March 1993 to May 2002 and undergoing hemodialysis through AV fistula, the 14 Patients in whom central vein stenosis arose were selected for the study. A total of 28 percutaneous interventions(5 PTA and 23 stent placement) were performed, and restenosis rate and the time taken till the restenosis in de novo lesions and instant lesions were compared. All 28 cases were operated successfully. The 14 cases that received both anigioplasty and stent placement initially. (de novo lesion : 14 cases), Among the 10 cases with de novo lesion that followed up more than 1 year, 3 cases are currently undergoing hemodialysis without restenosis, and the remaining 7 cases have recurred stenosis with the mean time to restenosis of 10.9 months. In the 7 cases in whom stenosis recurred, 11 interventions were done(instent lesion: 11 cases). 4 of these were using only ballon angioplasty with 100% restenosis rate and the mean time of 3 months until restenosis. The remaining 7 cases were using both balloon angioplasty and stent placement, also with 100% restenosis rate but with the mean time of 12 months until restenosis, which was later than the group receiving only balloon angioplasty. In treating the patients with central vein stenosis, stent placement seems to be more advantageous over PTA in terms of restenosis rate and the mean duration of patency. In the case of instent lesion, inserting the stent for the second time after stenosis recurred lengthened the duration of patency compared to performing balloon angioplasty alone.

S.D. Rats를 이용 1, 1-Dichloro-1-fluoroethane의 아민성 흡입독성 연구

김현영,이성배,임철홍,김철우,점용현,한정희,전윤석,최수영,강대봉,이용묵 한국환경독성학회 2002 환경독성보건학회지 Vol.17 No.1

There were no specific effects for test materials on Sprague-Dawley (S.D.) rats in clinical symptoms, amounts of food intakes, weight changes, laboratory findings, and pathology after whole body l, l-Dichloro-1 -fluoroethane (used as coolant, metal cleaner and solvents) exposure(0, 1,500, 3,000, and 6,000 ppm) for 13 weeks (6 hour/day, 5 days/week). However, the loss of capillary vessels in eyeball (pupil) was observed in a female rat among 6,000 ppm group. Though there was a tendency for MCHC (Mean Corpuscular Hemoglobin Concentration) in rat to be decreased (p<0.05), it was not regarded as abnormal because the values were within normal limits. In asthma-stimulation related evaluations, there was also a tendency for inflammatory cell counts in bronchoalveolar lavages to be increased. But it had no statistical significance, and also no dependency on sex and the exposed concentration. Based on this result, the non observed effect level (NOEL) induced by 1, 1-Dichloro-l -fluoroethene inhalation was evaluated in groups with 3,000 ppm below (S .D. Rats, 13 weeks). Finally, it was concluded that the short term exposal of 1, 1-Dichloro-l-fluoroethane is not considered as a asthma stimulant by inhalation despite of some study limitations such as test animals use and short-term exposure .

재이식 가능한 32비트 테스크 코어 설계

윤영상,최종화,김용대,한선경,유영갑 충북대학교 컴퓨터정보통신 연구소 2004 컴퓨터정보통신연구 Vol.12 No.1

본 논문에서 제시한 32비트 테스트 코어는 기존의 ARM과 같은 32비트 RISC 코어를 대신하여 JTAG 및 코어브레이커의 회로 테스트를 위하여 고안되었다. 테스트 코어는 디버깅유닛을 바꾸어가며 재이식(retargetable) 가능하게 사용될 수 있다는 장점을 갖는다. 회로 합성 결과 하드웨어는 ARM과 비교하여 33% 정도의 게이트를 가진다. 테스트 코어는 보다 높은 디버깅 성능을 위해 다중로드(multiple load; LDMA)와 다중저장(multiple store; STMA)명령을 추가하였으며 단일로드(single load; LDR) 또는 단일저장(single store; STR)명령들에 비해 64% 정도의 처리시간 단축을 나타내었다. A 32bit RISC type core is designed to replace full processors for testing JTAG and core breaker modules. It is retargetable and can be utilized to test a debugging unit. The design uses about 33% of gates with respect to a fully designed ARM7. It has instructions, multiple load or store(LDMA, STMA), for debugging purposes. Simulation result shows that they reduce the execution time about 64% with respect to processors having a single load or store(LDR, STR) instruction.

특수효과가 있는 디지털 비디오의 컷 검출

김대환,최연성,고남영 群山大學校 1996 論文集 Vol.23 No.-

Parsing video content is an important first step in the video indexing process. This paper presents algorithms to automate the video parsing task and the presentation key-frame extraction. In this study, we propose a cut detection algorithm using linear coefficients of correlation from edge-map and analyze the existing cut detection methods. Experiments applying to real video clips confirm our methods. Video data modify it for dissolve effect which is fade-out and fade-in. Finally we design a browsing system for video data that use our cut detection algorithm.

강원,경북지역 낙엽송 지위지수 적합성 평가에 관한 연구

이대성 ( Dae Sung Lee ),이의섭 ( Eui Sub Lee ),최종현 ( Jong Hyun Choi ),서영완 ( Yeong Wan Seo ),최정기 ( Jung Kee Choi1 ) 한국임학회 2014 산림과학 공동학술대회 논문집 Vol.2014 No.-

본 연구는 강원 및 경북지역의 낙엽송 지위지수의 적합도를 평가하기 위해 실시되었다. 연구자료는 강원 및 경북지역의 45개 낙엽송 인공림 표준지 조사로부터 우세목 1본을 선발하여 수간석 해를 통해 분석되었다. 산림청에서 개발된 지위지수표에 의거하여 본 연구대상지의 지위지수를 산정한 결과, 지위지수가 18 이하로 낮은 지역은 8곳(18%)이었으며, 지위지수가 20 이상으로 우수한 지역이 37곳(82%)으로서 대부분 지역에서 지위지수가 우세하였다. 지위지수곡선의 적합도를 평가하기위해 수간석해목의 과거에서 현재까지의 수고생장에 따른 지위지수의 변화를 도시한 결과, 초기 임령 때 지위지수와 차이가 없는 곳은 단지 8지역(18%)이었으며, 지위지수가 2∼4 m 차이가 발생한 지역이 19곳(42%), 6 m 이상 발생한 지역이 18곳(40%)으로 나타났다. This study was performed to evaluate the compatibility of site index for Larix kaempferi in Gangwon and Kyeongbuk regions. The data were collected and analyzed from stem alalysis of one dominant tree at each of 45 permanent plots installed in the regions. The result of site index estimation following the site index table developed by Korean Forest Service (KFS) showed that 8 plots (18%) were less than site index 18 and 37 plot (82%) greater than site index 20. Height growth changes of stemmed trees until present were illustrated in a graphic form to evaluate the compatibility of site index curve. The result showed that only 8 plots (18%) did not show any change of site index as trees age, 19 plots (42%) fluctuated in 2∼4m and 18 plots (40%) over 6m.

n-CdS_(0.46)Se_(0.54)/p-Cu_92-x)S_(0.46)Se_(0.54) 이종접합 태양전지의 제작과 그 특성에 관한 연구

유상하,최승평,이상열,홍광준,서상석,김혜숙,전승룡,윤은희,문종대,신영진,정태수,신현길,김택성,유기수 全北大學校 基礎科學硏究所 1994 基礎科學 Vol.16 No.-

승화방법에 의해 CdS_0.46Se_0.54 단결정을 성장하여 결정구조를 조사하고, Van der Pauw 방법으로 Hall effect를 측정하여 carrier density의 온도 의존성과 mobility의 온도 의존성을 조사하였다. 성장된 CdS_0.46Se_0.54 단결정을 치환반응하여 n-CdS_0.46Se_0.54/p-Cu_2-xS_0.46Se_0.54 이종접합 태양전지를 제작하였다. Spectral response, 전류-전압특성 및 전력변환 효율을 조사하여 그 결과로부터 개방전압은 0.48V, 단락 전류 밀도는 21mA/㎠, fill factor와 전력변환효율은 각각 0.75와 9.5%를 얻었다. CdS_0.46Se_0.54 single crystal was grown by a sublimation method. The crystal structure and the temperature dependence of carrier density and mobility of CdS_0.46Se_0.54 single crystal were studied. Heterojunction solar cells on n-CdS_0.46Se_0.54/p-Cu_2-xS_0.46Se_0.54 were fabricated by the substitution reaction. The spectral response, the J-U characteristics and the conversion efficiency of the n-CdS_0.46Se_0.54/p-Cu_2-xS_0.46Se_0.54 heterojunction solar cells were studied. The open-cricuit voltage, short-circuit density, fill factor and conversion efficiency of n-CdS_0.46Se_0.54/p-Cu_2-xS_0.46Se_0.54 heterojunction solar cells under 80mW/㎠ illumination were found to be 0.48V, 21mA/㎠, 0.75 and 9.5%, respectively.

생쥐의 바이러스성 심근염에서 합병증 동반 유무에 따른 조직 및 혈청내 금속단백효소억제자의 발현 차이

김성숙,엄대운,우영주,서재희,허주령,홍영미,최인표 대한병리학회 2001 Journal of Pathology and Translational Medicine Vol.35 No.3

膜分離에 의한 海水의 淡水化에 관한 硏究

申盛義,李性琪,崔炯一,申大允,金永範,李抵憲,姜永周 조선대학교 환경연구소 1989 環境公害硏究 Vol.6 No.-

In this study, we made investigation into water permeation, solute separation, concentration and Na^(+), K^(+), Mg^(2+), Ca^(2+), Cl^(-), SO^(2)_(4)-, HCO^(-)_(3) ions separation of seawater by the revers osmosis process using a suitable semipermeable membrane. The different thickness of membranes were prepared. On heat treatment, temperature was 80℃ and thier effects were also investigated. The flat single module system was made to be capable of treating feed solution at 200-2000cc/min and 120 atm. The experimental results can be summerized as follows: 1) As the effective operating pressure increased, permeability of pure water proportionally increased. 2) As the exposure period of the manufactured membrane in air increased, permeation rate increased, but as the thickness of the membrane increased, permeation rate decreased. 3) The capacity for being reproductive of the manufactured membrane (CA: 30wt%, Formamide: 45wt%, Acetone: 25wt%) in their performance was less than 10-30% and agreed with that reported in the literature. 4) We could separated about 85-95% of salt ion in seawater using a flat single module system.

8년간(1992~1999) 산업안전보건연구원에 의뢰된 직업병 심의 사례 분석

강성규,김규상,김양호,최정근,안연순,진영우,최병순,양정선,김은아,채창호,최용휴,김대성,박정선,정호근 대한산업의학회 2000 대한직업환경의학회지 Vol.12 No.2

Objectives : Pneumoconisis and noise-induced hearing loss (NIHL) have been reported as main occupational diseases by the Special Health Examination. The Industrial Accident Compensation Insurance has reported various work-related diseases, however, these two diseases occupied almost a half of compensated cases. Therefore, it was not well known about the status of occupational diseases other than pneumoconiosis, NIHL, and cardio-cerebrovascular accident (CVA). This study was conducted to analyze claimed cases as an occupational disease, that was requested to the Korea Industrial Safety and Health Agency (KOSHA). Methods : The local office of the Korea Labor Welfare Corporation (KLWC) has asked the KOSHA for confirmation of claimed cases as an occupational disease. We analyzed 379 cases requested from KLWC, the Ministry of Labor, employers, unions and occupational health agencies from 1992 to 1999. Results : Male was 80.7 % of the requested cases. Their mean age was 42 years old and 75.5 % of them were more than 35 years old. The requested cases were increased rapidly from 25 cases in 1992 to 108 cases in 1999 and the accept rate was 50.7 %. The majority of requested cases were respiratory diseases(22.4%), cancers(18.5%), Neuropsychiatric problems(14.5%), and musculoskeletal problems(13.5%). The accept rate was high in reproductive, respiratory, musculoskeletal and digestive disorders and low in neuropsychiatric, renal and otologic problems and occupational cancers. 73.6% of them were caused by chemical agents, especially 28.5 % were by organic solvents. 67% of them were clinically confirmed at university hospitals. A half of the cases were from KyongIn area, even the request came from the whole country. Conclusions : A claim was common in workers whose age was over 35 years old and exposure history was over 10 years. The respiratory diseases and neuropsychiatric disorders were still main problems in occupational health and occupational cancers was increasing even though its accept rate was not high yet.