인체구강상피세포의 배양조건에 대한 연구

박종수,최희웅,이수관,김수복,고광삼 中央醫學社 1990 中央醫學 Vol.55 No.2

Human epithelial cell cultures were initiated from explants of normal human oral mucosa. Growth parameter and cell type were assessed by DNA concentration and morphology of the cell lines. Tissues were excised and epithelial cells isolated by collagenase digestion. Spermine was used in order to inhibit overgrowth of the epithelial cells by fibroblast. The effects of different medium formulations were compared. Cultures maintained on the same volume of RPMI 1640 and Dulbecco's modified minimum essential medium containing 10 % fetal bovine serum, 10 ?g/ml of insulin, lng/ml of somatomedin and 10 ng/ml of epidermal growth factor showed optimal and maximal growth. For each cultured human oral epithelial cell the DNA content was in the- range of 5.7 pg to 6.6 pg. This culture system provides epithelial cells that can be used for a variety of dental and biochemical studies.

簡略系統特性定數에 의한 調相設備의 最適計算法

周秀元,金權洙,鄭亨煥 동아대학교 공과대학 부설 한국자원개발연구소 1985 硏究報告 Vol.9 No.1

This study aimed at seeking system characteristic constant in power system as simple as possible and, by using it, calculating optimum reactive power source. The results are as follow. 1. The theory employed in this research showed the excellence of obtaining system characteristic constant as fast as possible. 2. Any system characteristic constant reasonable to the system's flow condition, whether it was on heavy or light load, was to be obtained. 3. The validity of the theory was affirmed by some calculating examples in terms of model system.

조직공정성의 개념 및 조직시민행동과의 관계에 대한 이론적 고찰

안관영,변태수 尙志大學校産業經營硏究所 2002 産業經營硏究 Vol.12 No.-

This study is based on the customer's satisfaction and emphasizes the high class service and the importance of personal service-quality according to the customer's visiting for the hotel accomodations located at chun-cheon city area. Service environment quality has become one of major factors in the selection of various purchase decisions, In fact, the level of a server's service quality affects the hotel firm's performance and the server's quality is emphasized not only as key determinants of the customer's satisfaction but also as complements of the server's strategic variables. This study focuses attention on the need of guests and some changes affect resort hotel companied only at an individual level. Hotel industry should be changed to promote diffusion of innovations or the spread of new ideas or hotel service products for behavioral characteristics of the consumers visiting Chun-cheon area. In addition, they should conduct occasional consumer decision surveys to monitor their standing with guests.

실시간 화상처리 기법을 이용한 다물체 위치 추정에 관한 연구

신광수,장완식,민관웅 朝鮮大學校 機械技術硏究所 2005 機械技術硏究 Vol.8 No.2

본 연구는 실시간 고속 다물체 화상처리 기법을 개발하고자 한다. 최근 들어 비젼 시스템의 응용은 검사 및 로봇위치 제어등 여러 분야에서 급속히 증가되고 있다. 비젼 시스템을 이러한 응용분야에 적용하기 위해서 3차원 공간상 물체 위치 값을 CCD카메라로부터 얻어진 이미지 정보를 변환하는 것이 필요하다. 검사 및 로봇위치 제어 등에 비젼 시스템을 적용하기 위해서는 이미지 평면에서 물체의 중심위치를 아는 것이 필요하다 특히 3차원 강체들의 경우에는 형상을 나타내기 위해서는 여러 개의 큐들이 필요하므로, 여러 개의 큐들의 위치 값들이 하나의 이미지 평면에서 동시에 결정되어야 한다. 이러한 문제를 해결하기위해 여러 개 큐들을 동시에 처리하는 다물체 화상 처리 기법을 본 논문에서 제시하고 개발된 알고리즘의 타당성을 실험을 통하여 보여준다. This study is to develop the high speed multi-object image processing algorithm in real time. Recently, the application of vision system is rapidly increasing in inspection and robot's position control. It is necessary to transform the physical coordinate of object into the image information acquired by CCD camera, which is called image processing, in order to apply the vision system. Thus, it is necessary to know the position of object in the image plane to apply the vision system in the inspection and robot's position control. Particularly, in case of rigid body using multi-cue to identify its shape, each position of multi-cue must be calculated in an image plane at the same time. To solve these problems, the image processing algorithm on the position of multi-cue is developed. Finally, the experiments are performed to show the practicality of this algorithm.

카누선수들의 유산소성 트레이닝을 위한 운동강도 설정

김근수,홍관이 江原大學校附設體育科學硏究所 2002 江原大學校附設體育科學硏究所論文集 Vol.- No.25

This research is to examine the exercise intencities neccessary to make out training program to improve canoe player's aerobic capacity. The sample of this research were 21 man and woman players more than 16 years old who have canoe players career more than 2 years. By the maximum exercise to exhaustion all-out state by steady increase subordinate exercise method by Bruce's protocole, established following exercise strength in Jevel of heart rate and VO2 that appear at ventilation threshold. 1. Man player's exercise intensity appeared by 65~70% of maximum heart rate. 2. Woman player's exercise intensity appeared by 70~75% of maximum heart rate. 3. In case establish exercise intensity by VO2, appeared by 65~70% level of maximum VO2 intake amount together man and woman.

산업부산물을 이용한 중금속/유기성 슬러지의 고형화 및 용출특성에 관한 연구

전관수,김인환 嶺南大學校 工業技術硏究所 2004 工業技術硏究所論文集 Vol.32 No.1

This paper discusses the development of mixes for silica fume as stabilization/solidification agents and binders for industrial wastewater residue containing organic and heavy metal contaminants. The UCS(Unconfined Compressive Strength) gradually increased to 66.7% as the silicafume content increased to 15%. The leaching of TOC and chromium decreased as more OPC was substituted with silica fume. When the mix had 5% silica fume, it retained about 85% TOC, and chromium leached out 0.76mg-Cr/g-Cr in acidic solution. Also, microstructural studies of the solidified wastes through the scanning electron microscopy/energy dispersive spectroscopy (SEM/EDS) and X-ray diffraction analysis showed that the silica fume caused an inhibition to the ettringite formation which did not contribute to setting, but coated the cement particles and retarded the setting reactions. The results indicated that the incorporation of silica fume into the cement matrix minimized the detrimental effects of organic materials on the cement hydration reaction and contaminant leachability.

아시아 지역 폐렴 구균의 항균제 내성 현황 및 변화 양상 : Asian Network for Surveillance of Resistant Pathogens (ANSORP) Study

정숙인,김나영,손준성,기현균,고관수,서지연,장현하,김연숙,오원섭,백경란,이남용,송재훈,항생제 내성 감시를 위한 아시아 연합 (ANSORP) 대한감염학회 2004 감염과 화학요법 Vol.36 No.1

목적 : 1980년대부터 페니실린을 비롯한 β-lactam 계열과 비 β-lactam 계열 항균제에 대한 폐렴구균의 항생제 내성이 급격하게 증가하여 국내 뿐 아니라 세계적인 문제로 대두되고 있다. ANSORP 에서는 아시아 지역에서 폐렴구균의 항균제 내성과 그 변화 추이, 내성의 기전 및 위험인자를 분석하고자 본 국제 공동 연구를 시행하였다. 재료 및 방법 : 1999년 11월부터 2001년 8월까지 아시아 지역 12개국의 14개 기관에서 폐렴구균 감염증 환자의 임상 검체로 분리된 침습성 폐렴구균을 대상으로 액체배지 미량희석법을 이용하여 16개 항생제의 MIC를 구하였다. 위험인자의 분석을 위하여 폐렴구균 감염증 환자를 대상으로 의무기록지를 조사하였다. Quellung 반응을 통해 혈청형을 결정하였다. 결과 : 총 685균주 중 52.4%가 페니실린에 대하여 비감수성(중등도 내성 22.9%, 내성 29.5%)을 보였고, 그 빈도는 베트남(92.1%), 스리랑카(85.7%), 홍콩(67.9%), 한국(64.5%)의 순이었다. Erythromycin에 대한 비감수성 비율은 베트남(92.1%), 대만(87.7%), 한국(80.6%), 홍콩(76.8%) 등에서 높게 나타났고, 베트남, 대만, 한국, 홍콩 등의 erythromycin의 MIC_(90)이 32㎍/mL를 넘는 고도내성을 보였다. 홍콩, 싱가포르, 대만, 인도에서는 fluoroquinolone에 대한 내성 균주가 출현하였다. 전체 균주 중 다제내성 균주의 비율은 47.7%였다. 혈청형은 19형과 23형이 가장 많았고, 그 외 6형, 14형 등이 있었다. 페니실린 비감수성 감염증의 위험인자로는 5세 미만(OR=1.7; 95%, CI, 1.2-2.4; P=0.002), 기관지폐질환(OR=2.0; 95%, CI, 1.3-3.1; P=0.003), 악성종양(OR=2.3; 95%, CI, 1.2-4.6; P=0.015), 스테로이드의 사용(OR=2.8; 95%, CI, 1.1-7.4; P=0.032)이 확인되었다. 결론 : 침습성 폐렴구균 균주를 대상으로 한 본 연구의 결과 아시아 국가들에서 페니실린, β-lactam 및 macrolide에 대한 폐렴구균의 내성률 및 내성 정도가 급증하고 있음을 확인하였다. Background : Emergence of pneumococcal resistance became a global issue since 1990s. According to the ANSORP studies with clinical isolates and carriage isolates between 1996 and 1999, some Asian countries showed alarmingly high prevalence of resistance to penicillin and other antimicrobial agents. To investigate the changing trends of pneumococcal resistance, ANSORP study group has performed a multinational surveillance study with invasive pneumococcal isolates from Asian countries. Methods : All isolates from various invasive pneumococcal diseases were prospectively collected from 14 centers in 12 countries between November 1999 to August 2001. Broth microdilution tests with 16 antimicrobial agents were performed according to the NCCLS procedures. Serotyping was performed by means of Quelling reaction with use of group-specific antisera. Results : A total of 685 isolates were collected. Overall, 52.4% of invasive isolates from Asian countries were not susceptible to penicillin (intermediate (1), 22.9%, Resistant (R), 29.5%). Vietnam showed the highest prevalence of penicillin non-susceptibility (1 20.6%, R 71.4%) followed by Sri Lanka (1 71.4%, R 14.3%), Hong Kong (1 24.1%, R 76%) and Korea (1 9.7%, R 54.8%). China (I 19.8%, R 23.4%) and Malaysia (1 9.1%, R 29.5%) also showed remarkable increase in penicillin resistance compared with previous ANSORP data, which were less than 10%. Vietnam (92.1%), Taiwan (87.7%), Korea (80.6%), and Hong Kong (76.8%) showed high prevalence of erythromycin resistance. MIG_(90)S for ciprofloxacin were 4 ㎍/mL (Hong Kong) and 2 ㎍/mL (11 Asian countries except Hong Kong), respectively. Conclusion : Compared with previous data from ANSORP studies, antimicrobial resistance among invasive pneumococcal isolates has markedly increased in Vietnam, Sri Lanka, Taiwan, China, and Malaysia. Continuous surveillance of pneumococcal resistance in Asia is strongly warranted.

경안면 괴사성 근막염 : 증례보고

박관수,정기훈,김효언,정정권,윤규호,전인성 대한악안면성형재건외과학회 2001 Maxillofacial Plastic Reconstructive Surgery Vol.23 No.1

Necrotizing fasciitis is defined as infectious disease showing extensive necrosis of the superficial fascia with widespread involvement of the surrounding tissues and concurrent systemic toxicity. It is found commonly in the extremities, the trunk and the perineum, but shows extremely rare occurrence in the well vascularized area such as head and neck area. Occurred in the head and neck area, it is called "cervicofacial necrotizing fasciitis" (CFNF). It is a fatal disease which carries a mortality rate up to 60%. Early diagnosis, aggressive surgical debridement of necrotic tissues and massive antibiotics therapy are essential for achieving a favorable outcome. We present a case of cervicofacial necrotizing fasciitis in 35-year-old male patient with literature review.

야뇨증 환아들의 심리사회적 특성에 대한 다기관 연구 : 행동 및 정서 문제를 중심으로

조수철,김재원,신민섭,황준원,한상원,박관현,이상돈,김경도,김건석,서홍진,이유식,정재용,김영균,문두건,남궁미경,한창희,조원열,김영식,배기수,이종국,정우영,신의진 大韓神經精神醫學會 2005 신경정신의학 Vol.44 No.6

Objectives : The aim of this study was to examine the behavioral and emotional problems associated with nocturnal enuresis in Korean children. Methods : Three hundred eighteen children with nocturnal enuresis, together with their parents, completed the Child Behavior Checklist (CBCL), Disruptive Behavior Disorder Scale according to DSM-IV (DBDS), Children's Depression Inventory (CDI), State-Trait Anxiety Inventory for Children (STAIC), and Piers-Harris Children's Self-Concept Scale (PHCSC). Ninety-three normal students were selected as the control group. Results : Compared to the normal control group, the mean scores with regard to the withdrawn, social problems, attention problems, delinquent behavior, aggressive behavior, externalizing problems and total problems profiles were significantly higher in the nocturnal enuresis group according to the CBCL results. The nocturnal enuresis group also scored significantly higher in the ADHD and ODD profiles of the DBDS. The nocturnal enuresis group was more depressed and anxious than the control group according to the results of the CDI and STAI. The mean score of the PHCSC was significantly lower in the nocturnal enuresis group when compared to the normal control group. Conclusion : The results of this study suggest that children with nocturnal enuresis in Korea have clinically relevant behavioral and emotional problems. The findings support the link between nocturnal enuresis and psychopathology in Korean children.

TFT-LCD bus line용 ITO/AlNd의 접촉저항의 특성에 관한 연구

김동식,정관수 경희대학교 산학협력기술연구원 1999 산학협력기술연구논문집 Vol.5 No.-

최근 TFT-LCD용 panel이 대형화, 고세정화됨에 따라 저저항 배선 재료의 개발이 요구되고 있다 이에 따라 저저항 배선 재료에 대한 개발 및 ITO와의 접촉저항을 줄이기 위한 연구가 활발히 진행되고 있다. 본 논문에서는 DC magetron sputtering system을 이용하여 다양한 조건하에서 AlNd 박막을 성장하여 각각의 sample들은 SEM, AFM, XRD와 4-point-probe를 사용하여 그 특성을 조사하였고, AIND과 ITO간의 접촉저항을 측정하였다. 본 실험에서의 최적 조건은 120℃, 125W, 0.4Pa, 30 :SCCM(Ar), 350℃-20 min. annealing에서 얻을 수 있었고, 그때의 AlNd(2%wt.)의 저항값은 약 4 Ωcm 이다. AlNd과 ITO간의 가장 낮은 접촉 저항값의 조건은 300℃. Ar 30sccm. O_2 분위기였고 그 값은 약 110Ω㎠이었다