임신 및 각종 갑상선질환에서 갑상선 기능 판정에 관한 연구 : 혈청유리 T4의 진단적 의의에 관한 고찰 The diagnostic value of free thyroxine by RIA

이종철,유명희,윤휘중,신영태,정순일,조보연,이문호,이명철 대한핵의학회 1981 핵의학 분자영상 Vol.15 No.1

To evaluate the diagnostic accuracy of the measurement of free thyroxine(FT4) by radioimmunoassay, we measured free T4 and T4, T3, T3RU, TSH and TBG serum levels by radioimmunoassay in 18 healthy persons and 52 patients with various thyroid diseases and 11 normal pregnant women. The results are as follows. 1) In 19 cases of overt hyperthyroidism, T3, free T4 and FTI, T4/TBG ratio reflect hyperfunction in all cases. T4 is increased in 94%(18/19) and TBG and TSH are decreased in 79%(15/19). 2) In 8 patients with overt hypothyroidism, TSH is increased in all cases and free T4 and FTI is decreased in all cases. T4 is decreased in 87.5%(7/8), T3 is decreased in 75%(6/8) and T4/TBG ratio is decreased in 62.5%(5/8). 3) In 5 patients who are clinically in euthyroid state after treatment of hyperthyroidism, T4 free T4, FTI and TSH are in the normal range in all cases and T3 is normal in 60%(3/5) and slightly increased in 40%(2/5). 4) In 10 patients who showed clinically borderline hypothyroidism after treatment of hyperthyroidism, TSH is increased in all cases and free T4 and FTI are decreased in all cases, but T4 and T3, T4/TBG ratio are in the normal limit in all cases. So after treatment of hyperthyroidism, TSH, free T4 or FTI are recommended as optimal function test. 5) In normal pregnancy, free T4, FTI and T4/TBG ratio reflect normal function, but the other parameters unreliable due to the influence of increased TBG. Also TBG and TSH level in pregnancy is increased significantly compared with normal healthy control group. 6) The coefficients of correlation between T4 and FTI were 0.862(p〈0.001) and 0.685(p〈0.001) between free T4 and T4/TBG ratio. In most patients, diagnostic value of free T4 was comparable and even superior to FTI, so free T4 measurement can be used routinely with thyrotropin assay in the diagnosis of hypothyrodism or with T3 for the diagnosis of hyperthyroidism.

조사료원과 급여수준이 한우의 비육능력 (肥育能力) 및 도체성적에 (屠體成績) 미치는 영향

정태영(T . Y . Chung),김종민(C . M . Kim),이왕열(W . Y . Lee),선우훈희(H . H . Sunwoo) 한국영양사료학회 1994 韓國營養飼料學會誌 Vol.18 No.1

정상 임신에서 임신주기에 따른 갑상선기능의 변화

김원배,정재훈,윤보현,이석인,김민선,오태근,조보연,이홍규,고창순 대한내분비학회 1994 Endocrinology and metabolism Vol.9 No.3

It is well known that normal pregnancy is accompanied by a rise in serum concentrations of thyroxine-binding globulin(TBG) and human chorionic gonadotropin (hCG). Alterations of biochemical parameters of thyroid function are recognized during gestation and sensitive tests to evaluate the alterations easily are required. Therefore, a cross-sectional study was undertaken in 140 healthy pregnant women to evaluate the efficacy of free T_4 measured by 2-step RIA compared to other thyroid function tests and to confirm the changes of thyroid function according to the stages of normal pregnancy. The sensitivities of free T_4 index, free T_4(by 2-step RIA), T_3 and TSH were realtively high(99.3%, 93.6%, 92.9%, 83.6%, respectively) compared to those of T_4 and T_3 bead upgake(49.3%, 21.4%) during all stages of pregnancy. There were positive correlations between free T_4 index and free T_4 or total T_4(r=0.68, r=0.72; p$lt;0.001). The values of free T_4 index sharply decreased from 3.22+-0.10(meam +-SEM) during 6th-12th week to an plateau after 16th-20th week of gestation(p$lt;0.01). The serum concentrations free T_4 and T_3 bead uptake also significantly decreased from 1.65+-0.05 ng/dl, 24.7+- 0.7% during 6th-12th week to an plateau after 16th-20th week of gestation, respectively(p$lt;0.001), No differences were found in the changes of serum concentrations of T_3, T_4 and TSH according to the stages of pregnancy. In conclusion, it is adequate to measure some tests including free T_4 index and free T_4 to evaluate thyroid function during pregnancy. The thyroid physiology and changes of thyroid function according to the stages of pregnancy should be considered in the interpretation of thyroid function status during pregnancy(J Kor Soc Endocrinol 9: 183-189, 1994).

200 GeV/핵자 유황이온과 핵건판핵의 충돌에 의해 생성된 헬륨 파쇄핵의 극한파쇄 연구

김동철,송진섭,윤천실,정성헌,박인곤,김종오,김철수,김태연,이승희,조재희,천병구,김재률,김준원,김태익,박명렬,장한일,임인택 慶尙大學校 기초과학연구소 1992 基礎科學硏究所報 Vol.8 No.-

고에너지 중이온 원자핵과 핵건판의 충돌에서, 200GeV/핵자 유황이온에 의해 생성된 파쇄 헬륨핵(Z=2)의 실험실계의 방출각 분포는 표적핵에 무관한 회귀공식. dN=exp[a+k exp(η-y_b)]d[exp(η-y_b)]로 잘 표현된다. 여기에서 의사신속도 η=-ln[tan(θ/2)]이고, y_b는 실험실계의 입사입자(^32S)의 신속도이다. 이 공식에 의한 적합에서 k=-0.057±0.008로 얻어진다. 즉, 핵건판과 고에너지 중이온의 충돌에서 파쇄 헬륨핵의 exp(η-y_b)의 분포는 "극한파쇄" 현상을 잘 설명하고 있다. The angular distribution of emission angle θ of helium (Z=2) produced in the collisions of incident particles of 200 GeV/nucleon ^32S in nuclear emulsion is well expressed by dN=exp[a+k exp(η-y_b)]d[exp(η-y_b)] where the pseudorapidity is η=-ln[tan(θ/2)], the laboratory system primary rapidity is y_b, and k=-0.057+0.008. The shape of this frequency of occurrence distributions in terms of exp(η-y_b) attests to the validity of the concept of "limiting fragmentation" for helium projectile fragments produced in the projectile fragmentation regions of heavy ion collisions in nuclear emulsion.

돈 분뇨 액비에 미생물 첨가가 배추의 발아지수에 미치는 영향

김태일,유용희,정의수,양창범,김민균,Kim, T. I.,Yoo, Y. H.,Chung, E. S.,Barroga, Antonio J.,Yang, C. B.,Kim, M. K. 한국축산환경학회 2005 축산시설환경학회지 Vol.11 No.2

본 연구는 돈 분뇨 액비에 미생물 첨가가 배추의 발아지수에 미치는 효과를 구명하기 위해서 수행하였다. 돈 분뇨 액비는 107일 동안 폭기와 무폭기 상태로 액상비료를 제조하여 공시하였고 미생물 첨가는 $0.05\%,\;0.1\%,\;0.2\%,\;0.3\%$ 수준으로 하였다. $0.05\%$ 미생물 접종 처리구에서 식물 독성이 낮고 발아지수가 높게 나타났다. 첨가수준별 돈분 액비 이용시험에서 초기 돈 슬러리의 액비성상은 T-N 2,873 ppm, T-P 753 ppm, $NH_4-N$ 1,441.6 ppm, $NO_3-N$ 16.48ppm으로 50일이 되면서 폭기조건에서 T-N 3,672 ppm, T-P 164 ppm, $NH_4-N$ 183.87 ppm, $NO_3-N$ 21.97 ppm로 변화하였으며, 무폭기 조건에서 T-N 1,261 ppm, T-P 68 ppm, $NH_4-N$ 161 ppm, $NO_3-N$ 16.87 ppm로 나타났다. 미생물을 처리하지 않고 107일동안 폭기조건과 무폭기 조건으로 액비화한 시료의 발아지수는 각각 83과 40.4이였다. 돈 분뇨 액비를 무산소 제조시 $40\%$ 이상, 호기제조시 $50\%$ 이상의 발아지수가 개선되었다. 이와 같은 결과는 액비제조 처리가 무산소 제조보다는 호기제조 처리가 작물에 양호한 것으로 판단되어지며 이는 호기처리시 총질소의 함량을 높게 유지할 수 있도록 하는 결과인 것으로 사료된다. This study was carried out to investigate the effect of Synechocystis sp. KACC 91007 when added to a pig slurry or pig liquid fertilizer (PLF) on germination index (GI) of Chinese cabbage. The preliminary experiment involved the screening of inoculant levels which were; 0.05, 0.1, 0.2, and $0.3\%$, respectively. The $0.05\%$ level of inoculant was selected based on low phytotoxicity and high GI.. The PLF underwent a 107 day aerobic and anoxic processing conditions. The T-N, T-P, $NH_4$, and $NO_3-N$ concentrations of the untreated pig slurry were; 2,873, 753, 1,441.6, and 16.48 ppm, respectively. Using aerobic processing treatment, the fertilizer value of the PLF was 3,672, 164, 183.87, and 21.97 ppm, respectively. In contrast, the fertilizer value of the PLF processed under anoxic condition was reduced to 1,261, 68, 161, and 16.87 ppm. The GI value of the untreated PLF under aerobic and anoxic processing condition was 83 and $40.4^{*}\%$, respectively. With the addition of the $0.05\%$ microbial inoculant, the GI improved by more than 40 and $50\%$ respectively, when the PLF was processed under anoxic and aerobic conditions. The above findings proved that the aerobic processing of PLF for 107 days was better than anoxic and yielded higher T-N which is a macro-nutrient fertilizer material. Consequently, the addition of $0.05\%$ microbial inoculant resulted to a higher GI of the Chinese cabbage specifically under aerobic processing condition.

Bacteroides fragilis의 독소 유전자 분석 및 발병기전에 관한 연구

오희복,이근영,정경태,성원근 국립보건연구원 2000 국립보건원보 Vol.37 No.-

The antimicrobial peptide human cationic antimicrobial protein-18/cathelicidin LL-37 as a putative growth factor for malignant melanoma

Kim, J.E.;Kim, H.J.;choi, J.M.;Lee, K.H.;Kim, T.Y.;Cho, B.K.;Jung, j.Y.;Chung, K.Y.;Cho, D.;Park, H.J. Sookmyung Women's University Research Institute of 2011 여성과 건강 Vol.6 No.2

Background Recent evidence suggests cathelicidin LL-37 to be a growth factor for various human cancers such as lung cancer, ovarian cancer and breast cancer. However, the effect of LL-37 against malignant skin cancer has not been reported. Objectives To investigate whether the human cathelicidin LL-37 is involved in the carcinogenesis of various skin tumours. Methods Human cationic antimicrobial protein-18 (hCAP-18)/LL-37 production in several cell lines including HaCaT, a chronic myelogenous leukaemia (CML) cell line and various melanoma cell lines was examined using reverse transcription polymerase chain reaction and enzyme-linked immunosorbent assay. Immunohistochemical analysis of melanoma, nonmelanoma skin cancer and precancerous and benign skin lesions was performed. After adding LL-37 to a melanoma cell line, tumour cell proliferation, migration and invasion were investigated. Results Human malignant melanoma cell lines overexpressed hCAP-18/LL-37 mRNA and peptide compared with HaCaT and CML cell lines. Immuno- histochemistry showed that the peptide was strongly expressed in malignant melanoma and moderately expressed in squamous cell carcinoma, whereas basal cell carcinoma, precancerous lesions and seborrhoeic keratosis showed no or weak expression. LL-37 also stimulated melanoma cell proliferation, migration and invasion in vitro. Conclusions Cathelicidin LL-37 was primarily expressed in human malignant skin cancer. LL-37 promoted melanoma cell proliferation, migration and invasion in vitro. We report that an increase in the level of LL-37 is associated with malignant skin tumours such as malignant melanoma. These results highlight the importance of LL-37 in the malignant tendency of skin tumours.

AFLP Marker 를 이용한 한우의 유전자 지문 분석

정의룡,김우태,김연수,한상기 한국동물자원과학회 2000 한국축산학회지 Vol.42 No.4

This study was conducted to investigate genetic variability and to develop the breed-specific DNA markers of Hanwoo (Korean cattle) and to estimate phylogenetic relationships among Hanwoo, Chinese Yanbian cattle and other foreign cattle breeds (Holstein, Angus, Charolais and Hereford) using AFLP markers as a new DNA fingerprinting technique. Genomic DNA was digested with a particular combination of two restriction enzymes with 4 base (Mse I and Taq I) and 6 base (EcoR I and Hind III) recognition sites, ligated to restriction specific adapters and amplified using the selective primer combinations. In the Hanwoo breed, the number of scorable AFLP bands detected per primer combination varied from 37 to 102, with an average of 66.1 using 13 primer combinations. A total of 859 markers were generated and 568 bands were polymorphic (66%). Among the primer combinations, E35/H12, M11/T32, E38/H13 and E32/H11 primer combinations showed a high level of polymorphism with 0.93, 0.89, 0.86 and 0.83, respectively. Therefore, these primer combinations were effective for AFLP analysis of Hanwoo. The level of polymorphism of Hanwoo was similar to that of Chinase Yanbian cattle (65%) and higher than those of other foreign cattle breeds (48% to 55%). E35/H32 and T32/M13 primer combinations generated AFLP fingerprinting showing individual-specific of Hanwoo. M13/H13 and T32/M13 primer combinations produced breed-specific DNA markers when compared AFLP DNA bands between breeds. Three bands (0.84kb, 0.70kb and 0.64kb) identified in M13M13 primer combination and two bands (0.25kb and 0.24kb) identified in T32/M13 primer combination were found to be unique in Hanwoo. Therefore, these AFLP DNA bands could be used as putative breed-specific DNA markers for Hanwoo breed identification. In comparison of genetic distances, Hanwoo was the most closely related to the Chinase Yanbian cattle, followed by the Hereford and Holstein breeds. AFLP fingerprints may be useful for parentage testing, pedigree analysis, genetic variability and relationships and for the development of DNA markers linked to economic traits in Hanwoo.

Studies on Morphological Characteristics of Korean Taenia saginata taiwanensis from Korea(Cheju Strain)

Chung, W.C.,Lin, C.Y.,Fan, P.C.,Soh, C.T. INSTITUTE OF TROPICAL MEDICINE YONSEI UNIVERSITY 1993 YONSEI REPORTS ON TROPICAL MEDICINE Vol.24 No.1

The present report is of an extensive investigation on the morphology of the adult and cysticercus stages of Korea Taenia (Cheju strain). The data on the adult worm are based on seven worms (two with scolex and five without) obtained from three infected persons. The worms weighed an average of 19.3gm (12∼30gm) and averaged 432cm (303∼665cm) long with 760 (640∼954) proglottides. The scolices averaged 458㎛ (440∼457㎛)×805㎛ (785∼825㎛), sucker 326㎛ (310∼341㎛)×236㎛ (230×242㎛), and rostellum 109㎛ (108∼110㎛)×110㎛ (108∼110㎛). In the mature proglottid, there were 832 (640∼1,034) testes which covered the whole dorsal side of the proglottid except for behind the vitellaria. The two ovaries averaged 430㎛ (250∼610㎛)×301㎛ (175∼550㎛) on the left and 418㎛ (260∼630㎛)×296㎛ (175∼485㎛) on the right. The vitellariae averaged 589㎛ (410∼840㎛)×138㎛ (75∼185㎛) and the mehlis gland 93㎛ (75∼110㎛) in diameter. The cirrus pouch did not extent to the excretory vessel and the vagina had a sphincter. In the gravid proglottid, there were 18 (13∼24) uterine branches, 2 (0∼5) buds, and 59 (23∼102) twigs present on the left side. Those on the right side averaged 18 (13∼24), 2 (0∼5), and 61 (35∼101), respectively. The length, width and thickness of the inner shell (embryophore) of the fixed eggs were 32㎛ (26∼36㎛), 26㎛ (20∼30㎛), and 1.2㎛ (1∼2㎛), respectively. The oncospheres averaged 20㎛ (13∼24㎛)×16㎛ (12∼19㎛); all had six hooks. The corresponding figures for the unfixed or fresh eggs were 35㎛ (28∼51㎛), 29㎛ (24∼43㎛), 2㎛ (1∼3㎛), 27㎛ (20∼35㎛)×22㎛ (18∼28㎛) and 6 hooks, respectively. There were an average of 58,847 (31,540∼89,300) eggs in a gravid proglottid. The length, width and diameters of the protoscolex, rostellum, and suckers of the cysticerci were 1.6mm(0.4∼3.4㎛), 1.5mm (0.3∼3mm), 501㎛ (150∼1,528㎛), 89㎛ (4∼240㎛), and 192㎛ (90∼380㎛), respectively. There were two rows of rudimentary hooklets on the scolex of the cysticerci. The inner hooklets averaged 16 (1∼76) in number and 11㎛ (2∼28㎛) in length. The outer hooklets were numerous and appeared as small dots. The surface of the cysticerci had a tubercular appearanc. These biological and morphological characteristics of Korean Taenia are similar to other T. saginata-like tapeworms in Asia, and have been designated as a new subspecies T. saginata taiwanensis.

Clostridium difficile에 대한 분자역학 분석 방법 비교 연구

정경태,정예선,성원근,오희복 국립보건연구원 2000 국립보건원보 Vol.37 No.-