모래상자놀이를 통한 부적응 행동유아의 자아존중감 및 친사회적 행동 변화양상에 관한 연구

이연승(Lee Yeon Seung),유명순(Yoo Myeong Soon) 한국어린이미디어학회 2007 어린이미디어연구 Vol.6 No.2

본 연구는 모래상자놀이를 통한 부적응 행동유아의 자아존중감과 친사회적 행동의 변화양상에 관한 연구로서, 모래상자놀이가 유아들의 전인적인 성장발달을 위한 기초자료로 활용될 수 있는지 확인하는데 그 목적이 있다. 이러한 연구목적을 달성하기 위하여, 부적응한 행동유아가 모래상자놀이를 통하여 어떻게 자신을 표현하고 집중할 수 있는 기회가 늘어나고, 또한 자아존중감 향상에 도움을 받으며, 친사회적 행동 관계에 어떤 영향을 주는지에 대한 비교 분석을 하였다. 연구과정은 25주에 걸쳐 사전검사, 모래상자놀이실시, 사후검사 순으로 진행되었다. 본 연구의 결과는 다음과 같다; 모래상자놀이를 통한 부적응 행동유아의 자아존중감 변화양상은 하위요인인 지적 능력에서 유아의 자아존중감이 향상되었고, 유아개인별 자아존중감 변화추이에서 향상되는 변화를 보였다. 또한, 부적응 행동유아의 친사회적 행동 변화양상은 나누기, 도와주기, 협동하기 등 친사회적 행동이 향상되었고, 유아개인별 친사회적 행동 변화추이에서 향상되는 변화를 보여주었다. The purpose of this study is to investigate the self-esteem and pro-sociality of the maladjusted children and this study is synthetic with using quantitative research. This study is made to present the possibility of the play with sand box to be the basic material for building children’s personality. For this purpose, the practical case has been analyzed to understand the effect on children’s sociality and self-esteem, as well as the way of expression of themselves and the opportunity of concentration on themselves. The target of the researcher is the 5~6 year old children in N child-care center which is attached to welfare facility in J area in Busan. The children are distracted, disturb others and have difficulty of social management, revealing the problem with a external maladjustment and their low self-esteem. Their home backgrounds are also normal except for one child whose parents are divorced. The research has been made in a daily life without any variable factor. The period of this research is 25 weeks, from August 16, 2006 to February 16, 2007 and pre examination, processing program, post examination are operated in order. As qualitative study, Young sook Kim’s modification on the Pictorial Scale of Perceived Competence and Social Acceptance for Young Children by Harter and Pike were used. In addition, Won young Lee’s revision and its questionnaire paper and assist material on the "Skill Situation Measues" by Mcginnis and Goldstein were used as measuring tool of pro-sociable behavior. For qualitative analysis tools used for this study are as follows. The results of this study were as follows: First, there was an improvement of self-esteem in cognitive ability of the children which is one of the sub factors and difference in each children who played with sand box. Second, there was an improvement of pro-sociality in sharing, help, and collaboration and it was shown in each children who played with sand box. And there was a change in children's pro-Behavior.

Purge & Trap-GC를 이용한 의약품 필름코팅 정제 중 잔류용제에 관한 연구

장준식,이명자,소유섭,문춘선,이주헌,박희라,김진숙,강경모,이선옥,방성연,유미자,유문균,금오성,이병욱 식품의약품안전청 2000 식품의약품안전청 연보 Vol.4 No.-

의약품은 약물을 생체에 적풋하기 위하여 유효성분의 효과가 언제나 일정하게 확보되고 사응에 편리하도록 만들어지는 것이므로 유효썽분 이외에 약효에 영향을 주지 않는 성분이 첨가되는 경운가 많다. 이 때 사용되는 용매들은 제피의 광택 및 건쪼시간의 단축 등을 위하여 휘발점이 낮을 용매들이 주로 사용되어진다. 본 연구는 의약품 필름코팅정제 중 잔류용매 4종(chlorofonr benzen, trichloro ethylen, 1,4-dioxane)에 대한 변형된 pirge & trap-GC 장치를 이용한 동시분석방법을 개발하였으며, 각 표준품의 RSD 값은 chloroform 3.03%, benzen 3.17%, trichloroethylen 3.69% and 1,4-dioxane 3.41%였다. 또한 시중 유통중인 의약품 50종에 대하여 잔류웅매 양을 측정하였으며, 검출되는 잔류용매는 한 건도 없었다. This study nras carried out to develope the analytical method for the mixture of chlorefonn, benzen, trichloroethylen and 1,4-dioxane simultaneously and determine the remainingorgauic solvents in coating tablets by Purge & Trap-GC. The results were as follouFs ; 1. Chloroform, benzen, trio:tloroethylen and 1,4-dioxane separated by tenax #5 trap by HP-624GC column by terrlperature programming. The peaks were separated completely at retentiontime of 6.88min for chloroform, 8.21min for benzen, 10.38miu for trichloroethylen and 11.95minfor 1,4-dioxane. 2. Standard RSD were individually chloroform 3.03%, benzen 3.17%, trichloroethylen 3.69%and 1,4-diorane 3.41%. 3. 60 samples were not detrcted chloroform, benzen, trichloroethylen and 1,4-dioxane.

Novel Methylation Biomarker for Non-invasive Diagnostics in Lung Cancer

오태정,( Chang Hun Lee2 ),( Min Ki Lee ),( Yeul Hong Kim ),( Sang Yull Lee ),( Hyo Sung Jeon ),( Shin Yup Lee ),( Seung Soo Yoo ),( Jae Yong Park ),( Sung Whan An ) 대한결핵 및 호흡기학회 2012 대한결핵 및 호흡기학회 추계학술대회 초록집 Vol.114 No.-

To identify aberrantly hypermethylated DNA in lung cancer cells we established a genome-wide analysis for hypermethylation sites, namely Methyl DNA Isolation and Amplification (MeDIA) coupled-CpG microarray analysis. In the comprehensive methyaltion profiling analysis between human lung cancer, A549 cells and normal NHBE cells, we observed that several clusters of genes show a significant level of aberrancy in CpG island methylation pattern in cancer cells compared to normal cells. We further identified PCDHGA12 gene as a new marker of non-invasive diagnostics for lung cancer based on followings. 1) Transcription of PCDHGA12 gene is reactivated after treatment of A549 cells with demethylating agent. 2) Bisulfide clonal-sequencing reveals that CpG island of PCDHGA12 shows a distinctive differential methylation between two cell lines. 3) Pyrosequencing-based quantitative methylation assay for such region in tumor and non-tumorous tissues from lung cancer patients shows aberrant hypermethylation in 37 (92%) of the 40 tumor tissues. In clinical validation by pyrosequencingin induced-sputum of lung cancer patients (n=87) and healthy controls (n=51), we observed aberrant hypermethylation incident at significantly elevated level in samples derived from lung cancer patients. According to the optimal threshold calculated by ROC curve analysis, sensitivity and specificity of PCDHGA12 was 86.2% and 82.4%, respectively. PCDHGA12 methylation status could be a potential methylation biomarker alone or combined with others for the screen and the detection of relapse of lung cancer.

당뇨병과 동반된 화농성 간농양의 임상상 및 예후인자

오은숙,강무일,이원영,오기원,임동준,이소영,이정민,고승현,김성래,안유배,손현식,윤건호,차봉연,이광우,손호영,강성구 대한당뇨병학회 2000 임상당뇨병 Vol.1 No.1

연구배경: 감염질환은 혈관질환과 함께 당뇨병의 중요한 사망원인의 하나이며, 혈당조절이 불량할수록 감염 빈도가 증가됨은 잘 알려져 있다. 간농양의 발생이 있어서도 당뇨병은 중요한 기저질환 중의 하나이다. 일반적으로 간농양의 가장 흔한 원인균은 Escherichi coli라고 알려져 있으나, 최근 당뇨병을 가진 간농양 환자에서 Klesiella pneumoniae의 검출이 증가되고 있다. 또 최근 연구에 의하면 K.pneumonie가 간농양의 가장 흔한 원인균이며, K. pneumoniae에 의한 간농양은 K.pneumoniae 이외의 균주에 의한 간농양보다 당뇨병과 밀접히 관련되어 있다고 보고된 바 있다. 이에 저자들은 간농양 환자를 대상으로 당뇨병환자의 빈도를 알아보고, 그 원인균 및 특징을 비당뇨병 환자들의 경우와 비교해 보고자 하였다. 방법: 1992년 1월부터 1999년 6월까지 방사선학적 검사 혹은 수술에 의해 간농양이 확인되거나, 경피적 천자나 수술을 통한 배농액 배양, 혹은 혈액 검사에서 원인균이 증명된 182명의 환자를 대상으로 후향적 임상고찰을 실시하였다. 결과: 화농성 농양을 가진 167명의 환자(남:94명, 여:73명)중 당뇨병을 가진 환자는 54명 (남:30명, 여:24명)으로 32.3%를 차지하였다. 환자의 평균나이는 당뇨군에서 62.1 ± 13.4세, 비당뇨군에서는 55.1 ±15.5세로 당뇨군에서 높았으며(p=0.0021), 남녀간의 평균나이 비교 시 두 군 모두 여성의 나이가 의미있게 높았다. 비당뇨군에 비하여 당뇨군의 경우 저알부민혈증의 빈도와 aspartate transaminase가 증가되어있었다. 원인균주는 K. pneumoniae (당뇨군54%, 비당뇨군 39.1%), E. coli(당뇨군 17.5%, 비당뇨군 18.8%), Pseudomonas aeruginosa, Streptococcus viridans, Enterococcus 순이었으며 두 군간의 의미는 차이는 없었으나 K. pneumoniae의 경우 다른 균종에 비해 단독감염의 빈도가 높았다(82.8%). 사망률은 당뇨군에서 16.7%, 비당뇨군에서 7.1%로 당뇨군에서 의미있게 높았으며(p=0.019), 당뇨군에서의 사망환자는 모두 패혈증이 동반되어 있었다. 당뇨환자의 사망군은 생존군에 비해 당뇨병 이환기간이 길고 혼합감염의 빈도가 높았다(p=0.046). 검사실 소견에서는 총 빌리루빈 및 알카리성 포스파타제의 증가가 관찰되어 있었다. 결론: E. coli가 간농양의 주된 원인균이라는 기존 보고와는 달리 당뇨군과 비당뇨군 모두 K. pneumoniae가 간농양의 주된 원인균이었다. 또한 당뇨 유병기간이 길고 당뇨조절이 불량한 환자에서 혼합감염이며 총 빌리루빈 및 알카리성 포스파타제의 증가등과 같은 위험인자가 존재할 경우 사망률이 증가될 수 있으므로 이들 군에서는 적극적인 치료가 필요할 것으로 사료된다. Background: Liver abscesses are commonly associated with underlying disease, particularly diabete mellitus. The number of the liver abscesses caused by Klebslella pneumoniae in diabetic patient has been increased in Korea nowadays. This study was conducted to clarify the clinical presentation and prognostic factors of pyogenic liver abscesses, especially in diabetic patients and to determine the proportion of K. pneunomiae as a pathogen in liver abscess in Korea. Methods: Medical records of 167 patients treated for pyogenic liver abscess from January, 1992 through June, 1999 were reviewed retrospectively in detail. Major Pathogenic organism, clinical manifestations, prognostic factors, the importance of diabetes mellilus as an underlying disease and its effect on clinical features and prognosis were analyzed. Results: Among 167 cases of pyogenic liver abscess, underlying diabetes mellitus was present in 32.3%. The mean age of patients was 62.7 13.4 years in diabetic liver abscess group and 55.1 15.5 years in non-diabetic liver abscess group. Most liver abscesses were cryptogenic in origin or secondary to the billary tree diseases. The clinical presentations among the two groups were not significantly different. When compared to patients without diabetes, patients with diabetes had significantly higher proportions of hypoalbuminemia and elevated aspartate transaminase level. The most common organism of the pyogenic abscess was K. pneumoniae in both groups. Percutaneous drainage of the abscess with appropriate antibiotics was the most commonly used therapeutic modality in liver abscess. The mortality rate of diabetic liver abscess is 16.1 % and 7.1% in nondiabetic liver abscess. Complications, especially septicemla, were found more frequently in patients with diabetes than in patients without diabetes (64.8% vs 28.3%, septicemia : 31.5% vs 9.7%). The factors influencing mortality in the diabetic liver abscess were disease duration, mixed infection, presence of septicemia, elevated bilirubin and alkaline phosphatase. Conclusions: In contrast to prior report that the E. coli was the most common pathogen in liver abscess, we found that K. pneumoniae was the most common organism cultured in liver abscess. Diabetic patients have more complications and higher mortality than patients without diabetes. Early detection and proper treatment are needed to improve the outcome for diabetic patient with liver abscess.

악성 림프종 환자에서 발생한 다형홍반

여운철,조광현,이유신,허대석,이승철 ( Un Cheol Yeo,Kwang Hyun Cho,Yoo Shin Lee,Dae Seog Heo,Seung Chul Lee ) 대한피부과학회 1990 대한피부과학회지 Vol.28 No.5

A Case of Erythema Multiforme A ssociated with Malignant Lymphoma Un Cheol Yeo, M.D., Kwang Hyun Cho, M.D., Yoo Shin Lee, M.D., Dae Seog Heo*, M.D., Seoung Chul Lee**, M.D Department of Dermatology and Internal Medicine*, College of Medicine, Seoul National University, Seoul, Korea, Department of Dermatology**, College of Medicine, Inha University, Kyung Gi Do, Korea We report a case of erythema multiforme associated with malignant lymphoma. Fifty-six-year-old male patient visited our clinic complaining generalized skin lesions which were compatible with erythema multiforme clinically and histopathologically. The skin lesions were treatment-resistant, recurrent and persistent for 3 years. In consideration of old age, atypical features of erythema multiforme and no other demonstrable etiology of erythema multiforme, we studied for internal malignancy. Malignant lymphoma at the duodenum was revealed by UGI series, abdominal CT, and endoscopic biopsy. After chemotherapy, the tumor mass and active skin lesions were cleared simultaneously. Erythema multiforme may be regarded as one of the paraneoplastic syndromes.

미세절편체에 의한 절화류의 기내증식

이지영,이승우,김용구,정인식,설일환 慶熙大學校 1997 論文集 Vol.26 No.-

Experiments were conducted to find out the optimum cultural conditions for micro-cross sectioned leaf segments of Gypsuphila paniculata L. Gerbera hybrida and Dianthus caryophyllus. Survival percentages of micro-cross sectioned segments were the highest in gypsophila, followed by carnation. Gerbera showed on survived segments. In gypsophila, segments thicker than 400㎛ were required to obtain over 75% survival. The percentage of adventitious bud formation was much higher with 600㎛ segment as compared with 400㎛. The regeneration of micro-cross sectioned segments of gypsophila was not sensitively affected by the auxins at varying concentrations. The elongation of adventitious shoot was promoted by kinetin compared with BA. In segments of 600㎛ thickness, adventitious shoot formation was promoted by BA compared with kinetin Adventitious shoot formation or shoot growth was not affected by KNO_3 concentrations with the segments of 400㎛ thickness. But adventitious shoot elongation was promoted significantly with segments in 600㎛ thickness. KH_2PO_4 (170 mg/1, standard concentration of MS medium) was effective for adventitious shoot formation. MgSO_4 also promoted adventitious shoot formation myo-Inositol tended to be suppressed regeneration of gypsophila.

意思決定範圍(SDM)의 變化에 의한 組織成長모델

李壽陶,劉承東 慶北大學校 産業開發硏究所 1984 硏究報告 Vol.12 No.-

The scope of decision making (SDM) is the main indicator of what managerial structure is necessary for an organization at a particular point in its life. The question of that elements by which we can measure the SDM thus becomes a basic and crucial one. The SDM is the totality of menagerial problems that require decisions to be made so that the organization will be able to continue its existence. The expansin of SDM takes place through one of the following three dimensions; (a) the level and variety of the technology employed by the organization, (b) the variety of its products or services, (c) the geographical of the organization The managerial structure of an organization must accord with its SDM at a particular point in time. That is, different SDM corresponds to different sized organizations and require different managerial structures to be able to operate efficiently and effectively, and thus survive. When an organization is created it usually has one product or service line and operates only in one area. Only through an entrepreneurial structure can an organization be created and operated. Until it is finally and firmly established it has to continue to be operated in this structure. This structure, however, ceases to be suitable when the level of technology is high and the equipment is effectively utilized. In such cases the organization must operate within a functional structure. As the organization grows and diversifies into two or more distinctly different products or services, the structure of the organization should be decentralized by product lines. Finally, When the organization is operating in a variety of countries that differ from one another in their natural culture, it must be operated in a mutlistructure. This last structure is a federation of the three basic structures.

sfs1 유전자의 cAMP-cAMP receptor protein에 의한 발현 조절

이희영,이승진,유주순,최용락,정수열 東亞大學校附設遺傳工學硏究所 1997 遺傳工學硏究 Vol.- No.4

crp* 유전자가 도입된 대장균 MK2001(crp, cya::km)을 숙주로 사용하여 mal 유전자 발현을 촉진시키는 유전자의 하나인 sfs1(sugar fermentation stimulation)의 구조해석 결과에 의하면, 잠정적인 sfs1의 promoter영역에는 CRP단백질과의 결합영역으로 보이는 염기배열이 존재하였다. 본 실험에서는 sfs1 유전자의 cAMP-CRP에 의한 발현 조절을 확인하고자, lacZ와의 융합 유전자를 작성하였다. 작성된 융합 유전자는 cya결손주인 TP2010에서 cAMP의 첨가에 의해 B-galactosidase 활성이 크게 증가하였으며, Western blotting의 실험에서도 같은 결과를 나타냈다. in vivo에서 발현이 확인된 전사산물은 cAMP에 의해 전사 촉진이 일어났으며, CRP의 결합부위로 예상되는 DNA영역은 cAMP가 존재하면 CRP단백질과 결합하는 특성을 나타내었다. 이상의 결과로 보아, sfs1 유전자의 발현은 cAMP-CRP에 의한 전사촉진 현상을 받는 것으로 나타났다. We have cloned several E. coli sfs genes which stimulate mal gene expression with ??. One the sfs genes(pPVC2) was sequenced and potential CRP binding site is located in the upstream of the putative promoter in the regulatry region. In order to investigate the regulation of the sfs1 gene by the cAMP-CRP complex, we have constructed the sfs-lacZ fusion gene in this research. The overall transcriptional stimulations of sfs1 gene in the presence cAMP were confirmed by B-galactosidase activity and Western blot analysis of sfs1-lacZ fusion gene. Transcriptional regulation by cAMP-CRP was also confirmed by Northern blot analysis. End-labelled DNA of the DNA fragement in sfs1 regulatory region were used for gel retardation assay to examine the CRP-DNA complex in the presence of cAMP. Results here indicate that CRP binding site in the regulatory region of sfs1 gene is positive regulator for the expression of sfs1 gene.

아미노산 투여가 운동중 싸이클선수의 젖산 및 심박수에 미치는 영향

이승국,유병렬,김문희,김완조,이선장 韓國體育大學校附屬 體育科學硏究所 1992 韓國體育大學校附屬 體育科學硏究所論文集 Vol.11 No.1

The purpose of the study was to determine whether a 15.6mg.Kg ??' does of glutamate given one hour prior to incremental exercise test would reduce the lactate formation. Five well trained cyclists served as subjects in this experiment which included a placebo and glutamate trial. The subjects initially work on treadmill 200m/min for 3 minutes and here increased the workload by 20m/min every 3minutes until exhaustion. Blood lactate were collected from finger-tip. Subjects in the glutamate trial showed significantly lower lactate formation during exercise then they did in the placebo trial. In both groups no significant difference was found on heart rate. During the recovery lactate and heart, rate were unaltered in either the glutamate or placebo trials. In summary, glutamate ingestion may reduce lactate formation resultly improvement of performance.

펩타이드-Intergrase 융합 단백질의 재조합 활성

이나영,유승구 연세대학교 산업기술연구소 1996 논문집 Vol.28 No.2

Pepetide-Integrase fusion protein containing 17 additional amino acids at the amino terminal of bacteriophage Lambda Integrase was used for in vitro recombination assay and was found to have full recombination activity just as wild type Integrase. This result suggests the possibility that Integrase recombination system can be used to develop a novel peptide library system in which random peptides are displayed by DNA bound Integrase.