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- 저자 : ( Chang Hun Lee2 ) (검색결과 3 건)
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Novel Methylation Biomarker for Non-invasive Diagnostics in Lung Cancer
오태정,( Chang Hun Lee2 ),( Min Ki Lee ),( Yeul Hong Kim ),( Sang Yull Lee ),( Hyo Sung Jeon ),( Shin Yup Lee ),( Seung Soo Yoo ),( Jae Yong Park ),( Sung Whan An ) 대한결핵 및 호흡기학회 2012 대한결핵 및 호흡기학회 추계학술대회 초록집 Vol.114 No.-
To identify aberrantly hypermethylated DNA in lung cancer cells we established a genome-wide analysis for hypermethylation sites, namely Methyl DNA Isolation and Amplification (MeDIA) coupled-CpG microarray analysis. In the comprehensive methyaltion profiling analysis between human lung cancer, A549 cells and normal NHBE cells, we observed that several clusters of genes show a significant level of aberrancy in CpG island methylation pattern in cancer cells compared to normal cells. We further identified PCDHGA12 gene as a new marker of non-invasive diagnostics for lung cancer based on followings. 1) Transcription of PCDHGA12 gene is reactivated after treatment of A549 cells with demethylating agent. 2) Bisulfide clonal-sequencing reveals that CpG island of PCDHGA12 shows a distinctive differential methylation between two cell lines. 3) Pyrosequencing-based quantitative methylation assay for such region in tumor and non-tumorous tissues from lung cancer patients shows aberrant hypermethylation in 37 (92%) of the 40 tumor tissues. In clinical validation by pyrosequencingin induced-sputum of lung cancer patients (n=87) and healthy controls (n=51), we observed aberrant hypermethylation incident at significantly elevated level in samples derived from lung cancer patients. According to the optimal threshold calculated by ROC curve analysis, sensitivity and specificity of PCDHGA12 was 86.2% and 82.4%, respectively. PCDHGA12 methylation status could be a potential methylation biomarker alone or combined with others for the screen and the detection of relapse of lung cancer.
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간벌에 의한 서식지 구조와 양서,파충류 개체군의 특성 차이
박창득 ( Chang Deuk Park ),정지화 ( Ji Hwa Jung ),손승훈 ( Seung Hun Son ),황현수 ( Hyun Su Hwang2 ),이우신 ( Woo Shin Lee ) 한국임학회 2016 한국산림과학회지 Vol.105 No.2
본 연구는 간벌에 따른 서식지 구조와 양서·파충류 개체군 특성 차이를 파악하기 위해 2014년과 2015년 의 6월부터 9월까지 강원도 홍천군 매화산의 침엽수림과 활엽수림에서 각각 실시되었다. 산림의 수직구조를 파악한 결과, 모든 임상에서 간벌 이후 산림환경이 구조적으로 크게 변화하였다. 특히 간벌 후 중층 및 하층의 피도량이 감소한 반면, 도목잔존물의 수와 부피는 증가하는 경향을 보였다. 침엽수림과 활엽수림에 각각 2개 조사구, 총 4개 조 사구 내에서 간벌 전과 후 월별 1회씩 총 32번의 선형횡단조사를 통해 양서류는 5종 119개체, 파충류는 3종 5개체 가 확인되었다. 임상별 관찰된 평균 종수 및 개체수 차이를 파악한 결과, 침엽수림에서는 양서류의 평균 종수가 간벌 후 감소한 것으로 나타났으며, 특히 참개구리(Pelophylax nigromaculatus)와 북방산개구리(Rana dybowskii) 등 2종 이 차이를 보였다. 파충류는 관찰 간벌 후 모든 임상에서 관찰되지 않았다. 향후 간벌에 따른 양서·파충류의 개체 군 특성 차이를 파악하기 위해서는 간벌의 규모와 빈도, 강도 등 다양한 요인을 고려한 정밀 연구가 필요할 것으로 생각된다. This study was conducted to clarify the differences in habitat structure and amphibian and reptile (herpetofauna) populations between before and after thinning in the coniferous and deciduous forests from June to September 2014 and 2015 at the Mt. Maewha of Hongcheon, Gangwon province, Korea. Forest structure was dramatically changed after thinning. Coverage of understory and mid-story were decreased while volume and number of downed trees were increased after thinning. We used line transect sampling method on a total of 4 transect lines, 2 lines in each forest type. 119 individuals of 5 amphibian species and 5 individuals of 3 reptile species were recorded. Mean number of amphibian species were decreased after thinning in the coniferous forest. There was no observed reptiles after thinning in the coniferous and deciduous forests. Detailed monitoring with different area, frequency and intensity might be needed to clarify more precise differences characterisitics of herpetofauna by thinning.
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