The HBV DNA Amounts in Serum Have No relationship with ALT level and Hetergeneous Population Coexits in Chronic Hepatitis B Virus Infection

Chun, Yoon-Keun,Ha, Joo-hun,Hong-Jung-Woo,Oh, Soo-Myung,Kim, Sung-Soo 경희대학교 동서의학연구소 1999 INTERNATIONAL SYMPOSIUM ON EAST-WEST MEDICINE Vol.1999 No.1

Yoon-Keun Chun¹,Joohun Ha□Hong-Jung Woo□, Soo Myung Oh□,Sung Soo Kim□ ¹Department of Molecular Biology, College of Medicine,²Department of Surgery, college of Medicine,³Department of Internal Medicine, College of Oriental Medicine,and ⁴East-Weat Medical Reserch Institute,Kyung Hee University, Seoul, Korea. The HBV DNA Amounts in Serum Have No relationship with ALT level and Hetergeneous Population Coexits in Chronic Hepatitis B Virus Infection. Proceedings of International Symposium on East-West Medicine, Seoul. 212-230, 1999. -Hepatitis B is caused by hepadnavirus. Hepatitis B virus replicates through 3.5kb pregenomic RNA intermediate which is regulated by core promoter. Pathogenesis of hepatitis B virus has been bilieved the result of host immune response. But recently many studies have reported that high level of viral replication caused by mutation in core promoter might result in severs hepatitis. But these studies were performed in vitro, not in vivo. So there is yet debate about which factor, viral of host factor, is more important in pathogenesis of hepatitis B virus. So we measured real viral replication level in 204 chronic hepatitis B patients by quantifying HBV DNA from sera by our novel PCR-based more sensitive method, and compared these results with ALT level measured from same sera, which indicates liver cell damage. Surprisingly there are no significant correlation between HBV DNA quantity and ALT level. Then we cloned core promoter region. In SSCP, we found that many viral mutants coexist in one patient. Base on SSCP result, we chose main viral core promoter type in each patients, which is thought to determine overall viral replication level in this patient. Main type of core promoter region of each 41 patients were directly sequenced. And with these we measured promoter activity by luciferase assay system and compared promoter activity with on another. We found tha there were some differences in promoter activity according to core promoter sequences. And we constructed replication-competent viral constructs with core promoter from 41 patients and Transfected these into HepG2 cell and measured HBV DNA by southern blot. There were also differences in HBV DNA quantity according to core promoter sequences. On these all results we investigated correlation between the effect of HBV core promoter on viral replication in vitro and HBN DNA quantity, ALT level from sera of each patients. We found there is no significant correlation among them. As a result, we concluded that in determining severity chronic hepatitis B patients, host factors of each patient is more important rather than replicative activity of virus itself.

서울의 Penicillinase Producing Neisseria Gonorrhoeae 발생빈도(1996)

김재홍,황동규,전재홍,김윤석,김중환,김용준,이창균,임동진,김현수,조창근,김경문,박상훈,전우형,김희성,이호정,차명수,김갑형,김형석,김석우,황지환,박병순,권오상,이민수,송기훈,성소영,이인섭,부태성 대한화학요법학회 1999 대한화학요법학회지 Vol.17 No.2

Background : In recent years, gonorrhea has been panedemic and remains one of the most commom STDs in the world, especially in developing countries. Objective & Methods: For the detection of a more effective therapeutic regimen and assessing the prevalence of PPNG, we have been trying to study the patients who have visited the VD Clinic of Choong-Ku Public Health Center in Seoul since 1980 by means of the chromogenic cephalosporin method. Results: In 1996, 139 strains of N. gonorrhoeae were isolated, among which 53(39.0%) were PPNG. Conclusion: Our results suggests that after a peak of 74.3% in 1993, the prevalence of PPNG in Seoul is gradually declining.

Evaluation of Hot Deformation and Dynamic Recrystallization Behaviors of Advanced Reduced-Activated Alloy (ARAA)

Sang‑Wook Kim,Hyeon‑Woo Son,Taek‑Kyun Jung,Young‑Bum Chun,Yi‑Hyun Park,Ji‑Woon Lee,Soong‑Keun Hyun 대한금속·재료학회 2019 METALS AND MATERIALS International Vol.25 No.4

The hot deformation behavior of advanced reduced-activation alloy (ARAA) was investigated using hot torsion tests. Thefl ow stress decreased as deformation temperature increased and as strain rate decreased. The fl ow behavior demonstratedthe typical dynamic recrystallization (DRX). Based on the constitutive analysis of peak stress, the activation energy for hotdeformation was found to be 330.3 kJ mol −1 . Peak stress was analyzed as a function of the Zener–Hollomon parameter, andcalculated and experimental values were in good agreement. A DRX kinetic model for ARAA was derived with deformationconditions based on the Avrami-type model. It was confi rmed that the volume fraction of dynamically recrystallized grainsincreased as deformation temperature increased and as strain rate decreased. The necklace structure and grain boundarybulging were observed in the deformed microstructure of ARAA. The suggested DRX mechanism for ARAA during hotworking is discontinuous DRX.

舞踊 專攻生의 體格과 體型에 關한 考察 : 忠淸南道 所在 中學校 學生을 中心으로

전혜자,우상연,이입주 순천향대학교 기초과학연구소 2004 순천향자연과학연구 논문집 Vol.10 No.2

The aim of this study is to ciassify, compare and analyze charecteristics of physiques and somatotypes of some middle school students majoring in Korean Dance(15 students), Modern Dance(15 students) and Ballet(14 students) in Chung nam Province. In order to get some results this study has used the methods to mesure the elements of physiques(Height, weight, girth of chest, sitting height, length of arms, length of legs) and to mesure skinfold thickness of four parts, bone diameters of two parts and muscle girth of two parts. The results of this study are as follows. 1. The mean height are 160.03±5.47(Modern dancers' group), sitting heights are 86.07±3.20(Modern dancers' group) But there is no significant differences. 2. The mean weights are 50.19±6.34(Korean danders' group), chest girth are 79.29±5.34(Korean cancers' group) But there is no significant differences. 3. The mean length of arms are 76.26±3.13(Ballet group), length of legs are 102.24±3.76(Ballet group) But there is no significant differences. 4. The rankings of muscle girth in two parts of obdies are 1) Modern dancers' group 2) Korean dancers' group 3) Ballter cancers' group and there are significant differences in maximum circumferencer of upper arms(p<0.05).

중학교 일반학급 학생과 특수학급 학생의 체력검사 비교분석

우상연,전혜자 순천향대학교 기초과학연구소 2004 순천향자연과학연구 논문집 Vol.10 No.2

The purpose of the study was to compare regular and special class students' development of physical fitness factors throughout three years of middle school. Also the differences in physical fitness factors between girls and boys in the third year regular and special classes in middle schools were investigated. To obtain the data of physical fitness for this study, 100 students(regular class boys(n=25), regular class girls(n=25), special class boys(n=25) and special class girls(n=25) who enrolled in middle schools in Seoul were surveyed. This study provided the information for a diagnosis of regular and special class students' physical fitness levels. Results of this study provided the data for establishing the polices of the physical education and the basic data for setting a physical education curriculum.

묘지 조성사업지의 비탈면 보호공법에 관한 연구(Ⅰ) : 강릉시 시범 공설묘지 조성사업을 중심으로

전근우,유남재,차두송,이명종,박완근,한상균 강원대학교 삼림과학연구소 2000 Journal of Forest Science Vol.16 No.-

강릉시 시범 공설묘지 조성사업은 경사 30˚ 의 표고차가 심한 산지를 절 · 성토하여 계단식 묘역단을 조성하는 공사로 수직고 15m이상의 대절토 및 성토부가 산재하고 있다. 특히 묘역단장후 주변 유입수 및 표면 유출수에 의한 비탈면 붕괴, 묘역단 유실 등의 피해가 발생할 경우 보수 또는 보강이 사실상 어려운 실정이며, 사회적 여론으로 확대될 가능성이 크므로 비탈면 보호에 필요한 녹화공법을 검토하였다. 구체적인 연구내용으로는 설계도면 및 현장답사를 통한 문제 비탈면 추출, 비탈면의 물리적 특성과 식생의 생육상황 파악, 비탈면 주변부의 식생조사와 유용식생 선발, 비탈면의 토양조건 분석 및 비탈면 토사의 기본물성 조사 등이며, 이를 기초로 하여 비탈면 현황 및 문제점을 파악하고, 그 대안을 제시하였다. The construction of a public cemetery in Kangnung city includes terraced graveyard with cutting or banking in mountain which has severe altitude differences with a slope of 30˚. Therefore, there are scattered cutting and banking sections with vertical height more than 15m. Especially, if the slope failure or the loss of a graveyard happens after graveyard establishment due to surface flow and inflow around, it is difficult to repair, causing serious public censure. Accordingly. revegetation works were examined that need protection of slope. The specific content of the study was the selection of slopes requiring special care through blueprint and field investigation the measurement of the physical property of slope and the situation of vegetative growth, the investigation of vegetation in slopes and the selection of vegetation, and the analysis of soil condition of slopes and the physical condition of slope sediment. On the basis of investigations some alternatives were proposed.Key words: public cemetery, slope protection works, revegetation works

흰쥐의 위축된 저작근에 대한 간헐적 전기자극 효과

천상우,박병림,한두석 대한구강생물학회 1992 International Journal of Oral Biology Vol.16 No.2

This study was designed to evaluate the effect of intermittent electrical stimulation on atrophied masseter muscle in the rat(230-250g). Growing Sprague-Dawley rats(80g) were assigned to either a control(CONT), a soft dieted(SOFT), or a soft dieted plus intermittent electrical stimulation(SOFT-ES) group. CONT group was continued to receive an ordinary hard rat diet and SOFT group received an ordinary diet in a ground form, mixed with water in a proportion of 2:5, for 6 weeks. After 6 weeks of soft diet feeding in SOFT group, mechanical properties of contraction in the masseter muscle were measured by means of isometric force transducer and then electrodes were implanted to the masseter muscle for intermittent electrical stimulation(SOFT-ES group). In SOFT-ES group, electrical stimulation was applied directly to the masseter muscle for 2 weeks at supramaximal intensity, 0.3ms, 20Hz for 15 minutes every 6 hours. After electrical stimulation for 2 weeks, mechanical properties and fatigue tendency of the masseter muscle were observed. The results were as follows. 1. Twitch tension was no significant difference according to the mandibular position, however, tetanic tension at 11㎜ interincisal distance was larger than at 7㎜. 2. In SOFT group, tetanic tension and contraction time were decreased, and fatigue tendency was increased compared with the control group. 3. In SOFT-ES group, tetanic tension was increased and fatigue tendency was decresed compared with the soft dieted group, however, all mechanical properties of muscle contraction were similiar to those of the control group. 4. Effects of electrical stimulation in atrophied muscle were more prominant in tension-related properties than speed-related properties. These data suggest that intermittent electrical stimulation may be useful in prevention and treatment of muscle atrophy.

한글 문서 구조 분석에 관한 연구

우성준,김광호,유명춘,하진영 강원대학교 정보통신연구소 2008 정보통신논문지 Vol.12 No.-

Although information technology has been developed fast, large amount of machine printed document images are not properly handled due to immature document layout analysis techniques. Previous research has focused on character recognition itself to improve character recognition accuracy. Recently, however, some efforts are made to analyze complex document layout automatically. In this paper, we propose document layout analysis and understanding method for various Hangul document images. Skew detection is performed using projection profile and connected component layout information. Top-down and bottom-up methods are combined to analyze document layout. After text region and non-text region determination, table is further processed to extract row, column, and cell position. We got very high performance for skew correction, zone detection, and table processig from experiments with various Hangul document images.

Effects of Hydrogen Peroxide on Neuronal Excitability and Synaptic Transmission in Rat Substantia Gelatinosa Neurons

Son Yong 대한구강생물학회 2007 International Journal of Oral Biology Vol.32 No.4

The superficial dorsal horn, particularly substantiagelatinosa (SG) in the spinal cord, receives inputs fromsmall-diameter primary afferents that predominantly conveynoxious sensation. Reactive oxygen species (ROS) are toxicagents that may be involved in various neurodegenerativediseases. Recent studies indicate that ROS are also involvedin persistent pain through a spinal mechanism. In thepresent study, whole cell patch clamp recordings werecarried out on SG neurons in spinal cord slice of young ratsto investigate the effects of hydrogen peroxide on neuronalexcitability and excitatory synaptic transmission. In currentclamp condition, tert-buthyl hydroperoxide (t-BuOOH), anROS donor, depolarized membrane potential of SG neuronsand increased the neuronal firing frequencies evoked bydepolarizing current pulses. When slices were pretreatedwith phenyl-N-tert-buthylnitrone (PBN) or ascorbate, ROSscavengers, t-BuOOH did not induce hyperexcitability. Involtage clamp condition, t-BuOOH increased the frequencyand amplitude of spontaneous excitatory postsynaptic currents(sEPSCs), and monosynaptically evoked excitatory postsynapticcurrents (eEPSCs) by electrical stimulation of the ipsilateraldorsal root. These data suggest that ROS generated byperipheral nerve injury can modulate the excitability of theSG neurons via pre- and postsynaptic actions.

북한 청소년에 대한 이해

전우택,민성길 연세대학교 통일연구원 1997 통일연구 Vol.1 No.1