Collagenase와 esterase가 상아질 접착강도와 nanoleakage에 미치는 영향

정영정,한세현,김종철,이상훈,김정욱,김영재,장기택 大韓小兒齒科學會 2008 大韓小兒齒科學會誌 Vol.35 No.3

본 연구는 상아질 접착계면에서 collagenase와 esterase가 접착강도와 극미세누출에 미치는 영향을 살펴보기 위해 시행하였다. 발치된 치아의 교합면 상아질에 Single Bond 2(SB)와 Clearfil SE bond(SE)를 사용하여 상아질-레진 접착시편을 제작하고,시편을 인산완충용액(PBS)에 24시간(I),또는 PBS(II),collagenase 용액(III),esterase 용액(IV)에 4주간 보관 한 후 질산은 용액으로 염색하였다. 시편의 미세인장접착강도(μTBS)와 질산은 침투 면적을 측정하여 다음과 같은 결과를 얻었다. 1. SB군의 접착강도가 II∼IV군에서 SE군에 비해 낮은 값을 나타내었다(p<O.05). SB군은 II∼IV군의 접착강도가 I군에 비해 낮은 값을 보였으나(p<O.05),SE군의 접착강도는 I∼IV군간에 차이를 보이지 않았다(p>O.05). 2. SB군의 질산은 침투 면적이 SE군에 비해 높았으며(p<O.05),SB군과 SE군에서 질산은 침투 면적은 I ∼ IV군간에 차이를 보이지 않았다(p>O.05). 3. 접착강도와 질산은 침투 면적은 SE군의 I, II,III군에서 음의 상관관계를 보였다(p<O.05). The purpose of this study was to evaluate the effects of collagenase and esterase on dentin bond strength and nanoleakage. Resin composites were bonded to occlusal dentin of premolars with Single Bond 2(SB) and Clearfil SE Bond(SE). After the microtensile specimens were prepared and stored in PBS for 24 hours(I) or, PBS(II), collagenase(III), esterase(IV) solution for 4 weeks, the specimens were stained with silver nitrate solution. Microtensile bond strength(μTBS) and silver penetration area were measured and, the results were as follows: 1. For group II, III, and IV, the bond strengths of SB were lower than those of SB(p<0.05). The bond strengths of SB II, III, and IV were lower than that of SB I(p<0.05). There was no difference among the bond strengths of SE I ∼ IV groups(p>0.05). 2. Silver penetration areas of SB were higher than those of SE for all storage groups(p<0.05). In SB and SE groups, there was no significant difference of silver penetration area among I ∼ IV groups(p>0.05). 3. SE I, II, and III showed inverse relationship between the bond strengths and the silver penetration areas(p<0.05).

Electrical, Thermal, and Thermoelectric Transport Properties of Se-doped Polycrystalline Re2Te5

Se Woong Lee,Okmin Park,Hyun-Sik Kim,Won-Seon Seo,Sang-il Kim 대한금속·재료학회 2022 대한금속·재료학회지 Vol.60 No.12

Re2Te5 is considered a potential thermoelectric material because of its intrinsically low thermalconductivity, due to its complex crystal structure. Herein, a series of Se-doped Re2Te5 (Re2Te5-xSex, x = 0, 0.2,1, and 2) samples were synthesized, and their electrical and thermal transport properties were investigated. Pure orthorhombic Re2Te5 phases were successfully synthesized without any impurities for all compositions,and the continuous decrease in the calculated lattice parameters confirmed the substitution of Se atoms atthe Te sites. A maximum power factor of 0.135 mW/mK2was achieved for the sample with x = 0.2 at 880 K,mainly due to the increase in carrier concentration and electrical conductivity. The lattice thermalconductivity significantly decreased for all doped samples, which was attributed to the point defect phononscattering caused by Se doping. The thermoelectric figure of merit, zT reached a maximum value of 0.20 at880 K for Re2Te4.8Se0.2 (x = 0.2) sample, which was approximately 22% higher than that of the pristine Re2Te5sample. The weighted mobility, quality factor, and expected zT were calculated to evaluate the optimizationof the power factor and zT.

폐암과 동반되어 나타난 기관기관지병증 골연골형성증 1예

이영진,전현수,박시형,김모세,김현주,이승헌,이영민,이현욱,이현경 仁濟大學校 白病院 2010 仁濟醫學 Vol.31 No.-

Tracheobronchopathia osteochondroplastica (TPO) is a rare benign disease of unknown etiology characterized by multiple cartilaginous or bony submucosal nodules, which project into the tracheobronchial lumen. Although some disorders were reported combined with TPO, these seemed to be considered casual. We report a case of TPO that combined with adenocarcinoma of lung. She was 50-year-old non smoking woman presented with chronic cough with right chest pain. Lung mass on right lower lobe was found on chest x-ray, so lung cancer was suspected. Multiple scattered whitish small elevations from trachea to bronchial trees were found on bronchoscopic exam. We mistook these lesions for metastases from lung cancer and biopsies of these lesions were done. Transbronchial biopsy for right lower lobe mass lesion was also done. But biopsies from trachea and bronchus revealed TPO rather than lung cancer. Transbronchial lung biopsy of right lower lobe mass was diagnosed adenocarcinoma of lung. So TPO combined with adenocarcinoma of lung was diagnosed.

상아질 접착에서 collagenase와 esterase가 미세인장결합강도에 미치는 영향

정영정,현홍근,김영재,김정욱,이상훈,김종철,한세현,장기택 대한소아치과학회 2007 大韓小兒齒科學會誌 Vol.34 No.2

상아질-레진 접착강도에 대한 collagenase와 esterase의 영향을 살펴보기 위해, 소구치의 교합면 상아질에 Single Bond 2와 Clearfil SE Bond로 접착을 시행하고 미세 시편을 제작하여 PBS, collagenase 용액, esterase 용액에 4주간 보관한 후 미세인장결합강도를 측정, 비교하여 다음과 같은 결론을 얻었다. 1. 모든 보관 용액에서 Single Bond 2의 미세인장결합강도는 Clearfil SE Bond보다 유의하게 낮았다(p<0.05). 2. Single Bond 2의 미세인장결합강도는 collagenase군이 PBS군, esterase군보다 낮았다(p>0.05). 3. Clearfil SE Bond의 미세인장결합강도는 esterase군이 PBS군에 비해 낮았으나(p>0.05), collagenase군보다는 높았다(p>0.05). Collagenase군은 PBS군에 비해 유의하게 낮은 미세인장결합강도를 보였다(p<0.05). The purpose of this study was to evaluate the effect of collagenase and esterase on the microtensile bond strength (μTBS) in dentin bonding. After resin composites were bonded to occlusal dentin, μTBS specimens were formed and stored in PBS, collagenase, or esterase solution. After 4-week storage, μTBS was determined and, the results were as follows: 1. μTBS values of Single Bond 2 were lower than those of Clearfil SE Bond for all storage medium (p<0.05). 2. In single Bond 2 group, collagenase solution lowered bond strength more than PBS and esterase solution (p>0.05). 3. In Clearfil SE Bond group, esterase solution lowered bond strength more than PBS(p>0.05). Collagenase solution lowered bond strength more than esterase solution(p>0.05) and PBS(p<0.05).

급성 파라쿼트 중독에서 혈중 파라쿼트 농도

박승민,김세현,최수진,김현,이완구,김영남,이광영,이영희,신성혜 대한응급의학회 2000 대한응급의학회지 Vol.11 No.3

Background: Paraquat is a bipyridyl compound, and when ingested, concentrated paraquat can cause either rapid death from multisystem failure and cardiovascular shock or delayed death from progressive pulmonary fibrosis. Paraquat is poorly absorbed by inhalation, but when ingested orally, severe illness can occur. Death usually occurs within 2 days if more than 50 ㎎/㎏ of paraquat is ingested . The most important prognostic indicator is the quantity of paraquat absorbed, as shown by the plasma paraquat concentration, and the prognostic indication depends mostly on the description given by the patients and their families about the amount of paraquat ingested, which is often underestimated or overestimagted. For these reasons, we tried to compare the plasma paraquat concentrations with amount of paraquat described by patients or their families. Methods: We reviewed the medical records of 59 patients with acute paraquat poisoning from February 1998 through February 1999, the paraquat concentrations in plasma were measured at Presbyterian Medical Center by using high performance liquid chromatography. Results: There was a striking discrepancy between the plasma paraquat concentration and the ingested amount described by the patients or their families. Conclusion: We recommend that the plasma paraquat concentration be measured in patients being treated for acute paraquat poisoning.

관상동맥 시술 도중 발생한 좌주간부 관상동맥 박리에 대한 스텐트 삽입 치료의 단장기 임상 결과 : acute and long-term results

이세환,이승환,홍명기,김영학,이철환,한기훈,송종민,강덕현,송재관,김재중,박성욱,박승정 대한내과학회 2004 대한내과학회지 Vol.66 No.6

목적 : 심도자 사용 시술과 관련된 좌주간부 관상동맥박리에 대한 적절한 치료는 아직 확실히 정립되지 않은 상태이다. 이에 본 연구는 좌주간부 관상동맥 박리에 대한 스텐트 삽입술의 단장기 임상결과를 후향적으로 분석하였다. 방법 : 좌주간부 관상동맥 박리에 대한 치료로 즉각적인 스텐트 삽입술을 시행하는 것이 안전하고 효과적인 방법임을 가설하였고, 이러한 즉각적인 스텐트 삽입을 시행한 10명의 환자를 대상으로 단장기적 임상경과를 의무기록 고찰과 전화 면담을 통해서 평가하였다. 결과 : 전체 환자 모두 처음부터 좌주간부 관상동맥에 유의한 협착을 가진 환자는 없었으며, 8명의 환자는 심도자의 조작으로 인한 박리가 발생한 경우였고(진단적 혈관 조영술 중에 3명, 유도 도자 조작 중에 5명), 나머지 2명은 다른 병변에 스텐트 삽입시술을 하는 중에 박리가 발생하였다. 이 10명의 환자에서 모두 즉각적인 스텐트 삽입술이 시행되었고, 4명의 환자에서 시술 도중 혈압 저하로 Intra-aortic Ballon Pump (IABP)를 장착하였다. 모든 환자에서 성공적으로 스텐트가 삽입되었고, 병원내 사망은 1명도 없었다. 6개월 추적 관상동맥 조영술은 8명의 환자에서 시행되었다. 혈관 조영상의 재협착(직경 협착 50% 이상)은 8명 모두에게서 관찰되지 않았으며, 퇴원 후 평균 31±25개월의 추적관찰 결과 주요 심장사건은 1건도 발생하지 않았다. 결론 : 비록 대상 환자가 적은 연구였지만 좌주간부 관상동맥 박리에 대한 스텐트 삽입술은 기술적으로 안전하고 빠르게 시행 할 수 있으며, 훌륭한 단장기적인 치료 효과를 보여준다. Background : The optimal treatment of patients with left main coronary artery (LMCA) dissection during catheter-based procedure remains uncertain. We retrospectively analyzed the acute and long-term results of bail-out stenting for LMCA dissection. Methods : In cases with significant LMCA dissection occurring during catheter-based procedure, prompt stent implantation may be safe and associated with favorable clinical outcome. We evaluated the acute and long-term results of bail-out stenting for LMCA dissection occurring during catheter-bases procedure in 1- patients. Results : Initially, there was significant stenosis of LMCA segment in these patients. Catheter-induced dissection occurred in 8 patients (during diagnostic angiography in 3 patients and guiding catheter manipulation in 5 patients). Two patients suffered dissection in the setting of stent deployment in other vessels. Therefore, bail-out stenting LMCA dissection was performed in a total of 10 patients. In 4 patients, hypotension developed and intra-aortic balloon pump was placed during procedure. Stents were successfully deployed in all patients. There was no in-hospital mortality. Six-month angiographic follow-up was performed in 8 patients. Angiographic restenosis(diameter stenosis 50%) was not observed in all patients at follow-up study. During a mean follow-up of 31±25 months after hospital discharge, there was no major adverse cardiac event (death, myocardial infarction, and target lesion revascularization). Conclusion : Bail-out LMCA stenting is technically feasible, and showed good acute and long-term results in a small series of patients.

β-Carotene 이 혈중 효소활성도에 미치는 영향

이상한,우기민,조만희,장예진,김창세,김현철 순천향의학연구소 1997 Journal of Soonchunhyang Medical Science Vol.3 No.2

This study was carried out in order to observe the effects of ethanol and β-carotene on the cholesterol, Triglyceride, LDL-cholesterol, HDL-cholesterol, glutathione peroxidase and catalase activities. The normal white S.D rats were classified into 4 group: 0.9% NaCl administration and 20% ethanol administration were referred to as group A, 20% ethanol and 50% β-carotene mixture administration was group B and 50% β-carotene administration was group C for the 30 day-feeding experiment. The results were obtained as following: 1. The blood lipid concentration was most highly increased in group A, was almost normal in group B, but significantly decreased in goup C. 2. The blood antioxidant enzyme activities were significantly decreased in group A, but significantly increased in goup B and C. 3. The blood β-carotene concentration were significantly decreased in group A, and slightly decreased in group B. Surprisingly it was increased in group C.

뇌종양 환자의 제왕절개술을 위한 전신마취 : 증례 보고 A case report

이헌근,전세현,손주태,신일우,정영균 대한마취과학회 2002 Korean Journal of Anesthesiology Vol.43 No.4

A 28-year-old primigravida at 33 weeks gestation was referred to our hospital because of aggravation of seizures which occurred intermittently for the past 2 years. A brain magnetic resonance imaging scan taken at 33 weeks gestation showed a large brain tumor (7 × 5 × 4 cm) in the left high frontal lobe. She had a cesarean section under general anesthesia at 38 weeks and 1 day gestation. General anesthesia was induced with thiopental 250 mg, succinyleholine 75 mg and lidocaine 60 mg after a vecuronium 0.8 mg intravenous injection. The hyperventilation and mannitol used to reduce increased intracranial pressure (ICP) in this patient may be harmful and detrimental to the fetus before delivery. Achieving both ICP control and fetal well being at the same time during a cesarean section can be challenging to anesthesiologists. We report the anesthetic management of a cesarean section in a patient with a large brain tumor. (Korean J Anesthesiol 2002; 43: 515~519)

허혈성 장질환의 조기진단을 위한 혈청 락테이트(lactate)의 유용성

이미진,오동렬,박규남,김세경,양진홍,박상현 대한응급의학회 2000 대한응급의학회지 Vol.11 No.4

Background: Intestinal ischemia remains a devastating event despite improvements in clinical recognition and in diagnostic and therapeutic modalities. The ischemic bowel diseases encompass a wide clinical spectrum from mild, reversible disease to severe, irreversible injury. The clinical picture is characterized initially by poorly localized visceral pain. Thus, it is difficult to diagnose this ischemia at an early stage. This study investigated whether an increased serum lactate level is a recognized danger signal marker for intestinal ischemia in patients who present at the emergency department because of abdominal complaints. Methods: Patients who came to our emergency department with abdominal pain and the risk factors of intestinal ischemia between Apr. 1999 and Nov. 1999 were included in this study. The data analysis included age, sex, final diagnosis, pathogenesis of bowel ischemia, and serum lactate level. Results: The serum lactate level in the intestinal ischemia group was 28.54±22.51 mg/dl; in non-ischemia group, it was 15.49±22.52 mg/dl. This difference between the two groups was significant(p<0.05). An increased serum lactate level had a sensitivity of 88.2% and specificity of 59.2%, a positive likelihood ratio of 4.92, and a negative likelihood ratio of 0.47 as a marker of bowel ischemia. These results do not represent a very meaningful revision of bowel ischemlc provability, but may make a small contribution to management of the disease, depending upon their magnitude and the clinical context in which they are applied. Conclusion: In patients with abdominal complaints, an increased serum lactate level is usually a useful aid as a diagnostic marker of bowel ischemia.

멸치젓의 呈味成分

李應昊,金世權,錢重均,金洙賢,金程均 釜山水産大學校 1982 釜山水産大學 硏究報告 Vol.22 No.1

멸치젓의 呈味成分에 관한 기초자료를 얻고자 생멸치와 이를 原料로 하여 제조된 멸치젓 중의 核酸關聯物質, 유리아미노산, betaine, TMAO, TMA 및 總creatinine을 分析하였다. 核酸關聯物質 중 생멸치에서는 IMP가 乾物量基準으로 2.8 μmole/g로 가장 많았으나 멸치젓에서는 hypoxanthine이 4.2 μmole/g으로 가장 많았고 , ATP는 흔적량에 불과 하였다. 멸치젓 중의 유리아미노산 중 함량이 많은 것은 leucine, isoleucine, phenylalanine, lysine, tyrosine, alanine, histidine, valine, methionine의 順이었고 glycine, proline, glutamic acid, arginine, threonine, serine 등은 함량이 적었다. 그리고 全유리아미노산은 젓갈 숙성중생원료에 비하여 약 2배로 增加 하였다. Betaine 은 생멸치 및 멸치젓 중에 各各 10.2㎎/100g, 30.0㎎/100/g였으며 이들이 엑스分室素에 대한 比率은 各各 0.6% 및 1.2%였다. TMAO 含量은 생멸치가 13.9㎎/100g, 멸치젓이 9.0㎎/100g였으며 TMA 含量은 各各 22.7㎎/100g 및 30.9㎎/100g였다. 總 creatinine 含量은 생멸치 및 멸치젓에서 各各 433.1㎎/100g, 575.8㎎/100g로서 엑스分室素에 대해 27.2% 및 22.8%였다. Omission test 結果 멸치젓의 呈味成分으로서는 유리아미노산 및 核酸關聯物質이 중요한 구실을 한다는 것을 알았다. This study was conducted to evaluate the taste compounds of fermented anchovy. The contents of such compounds as nucleotides and their related compounds, free amino acids, betaine, TMAO and total creatinine raw and fermented anchovy were analysed. The contents of IMP in raw anchovy appeared higher than other nucleotides and tended to decrease rapidly during fermentation. Therefore, in the case of fermented anchovy, hypoxanthine was abundant and IMP was very low in content. In the free amino acid composition of fresh samples abundant amino acids were leucine, isoleucine, phenylalanine, lysine, tyrosine, alanine, histidine, valine, methionine in order. Such amino acids as glycine, proline, glutamic acid, arginine, threonine and serine were poor in content. The contents of total free amino acids reached approximately about two times as compared with that of raw sample. The amount of betaine and TMA increased, while TMAO decreased during fermentation. The content of total creatinine in raw and fermented anchovy was occupied 22.8% and 27.2% of total of extractive nitrogen, respectively. As the results of omission test, the taste-active compounds of fermented anchovy are assumed to be free amino acids and nucleotides.