간 세포암에서 VEGF, TGF-β1, b-FGF 발현의 의의

김성용,남충현,주종우,채만규,백무준,이문수,김형철,안현철,김홍수,김창진,김창호 순천향의학연구소 2001 Journal of Soonchunhyang Medical Science Vol.7 No.1

Purpose: Angiogenesis is important for the proliferation and the metastasis of solid tumors. The growth of a solid tumor is widely recognized to depend on the process of neovascularrozation. Without angiogenesis, tumors cease to grow beyond even a few milimeters in diameter. It has been shown that tumor vascular density is an independent prognostic marker in several types of human tumors and is known to correlate with poor prognosis. To date, many angiogenic factors have been identified, such as transforming growth factor-α(TGF-α), transforming growth factor-β(TGF-β), fibroblast growth factor family(FGF), vascular endothelial growth factor(VEGF), platelet derived endothelial cell growth factor(PD-ECGF), tumor necrosis factor-α(TNF-α), and angiogenin. Hepatocellular carcinoma(HCC) is the second most common tumor in Korean males and is known as a typical hypervascular tumor with frequent portal vein invastion. The authors identified the expreesion of VEGF, TGF-β1, and b-FGF in HCC specimens and evaluated the relationship between these growth factors and the clinicopathologic characteristics of HCC. Method: We reviewed the medical records of 30 patients who were diagnosed as hepatocellular carinoma treated with hepatic resection between January 1994 and December 1998 in Soonchunhyang University Chunan Hospital. The selection of the cases was decided according to the condition of paraffin block fixation. The prognostic factors such as age, sex, tumor size, concentration of serum α-fetoprotein, presence of liver cirrhosis, presence of tumor emboli in portal vein, TMN stage, amount of transfusion during the operation, hepatitis B virus(HBV) infection, and Edmonson-Steiner(E-S) grade were investigated. Relationship between the prognostic factors and the immunopathologic expression of the TGF-β1, b-FGF, and VEGF was examined. Result: Thirty patients (24 males, 6 females) were included in the current study. The patient's mean age was 50.6 years and the age ranged from 36 to 65 years. The mean size of the tumor was found to be 5.2cm. All the patients were follewed up for 7 to 63 months. Child's classification A patients were 23(76.7%)cases, B patients were 7(23.3%)cases, and C was none. Immunohistochemical staining of HCC tumor mass in VEGF expression patients were 17(56.7%), b-FGF expression patients were 10(33.3%), and TGF-β1 expression patients were 10(33.3%). VEGF expression or more than one positive expression among the three factors correlated with tumor size and the stage of HCC but did not correlated with other clinicopathological characteristics. TGF-β1 and b-FGF did not correlate with any clinicopathological characteristics. Conclusion: The results suggest that the expression of VEGF or more than one positive expression among the three factors in HCC cells may be a significant prognostic factor of HCC.

신축학교 실내공기질이 초등학생들의 인지기능에 미치는 영향

김태섭,김선주,박시영,전만중,김규태,김창윤,정종학,백성옥,사공준 대한산업의학회 2007 대한직업환경의학회지 Vol.19 No.1

목적: 신축초등학교와 대조학교를 선정하여 교실 내 포름알데히드와 TVOCs를 측정하고 컴퓨터 신경행동검사를 이용하여 실내공기 오염물질이 학생들의 인지기능에 미치는 영향을 평가하였다. 방법: 신축초등학교 5학년 71명을 실내공기 오염물질 노출군으로 하고, 개교한지 12년째인 대구시의 일개 초등학교 5학년 2학급 63명을 대조군으로 하여 실내공기 오염물질농도 측정 및 컴퓨터 신경행동검사를 실시하였다. 결과: 신축학교에서 의도적으로 창문을 개방한 학급에서 1교시 포름알데히드 농도는 34.6 ㎍/㎥, 4교시에는 27.3 ㎍/㎥, 창문을 개방하지 않은 학급에서 1교시 80.2 ㎍/㎥, 4교시 127.1 ㎍/㎥, 대조학교에서는 2학급 각각 1교시에 12.6 ㎍/㎥, 7.5 ㎍/㎥, 4교시에 19.8 ㎍/㎥, 25.2 ㎍/㎥였다. TVOCs의 경우 신축학교의 의도적으로 창문을 개방한 학급에서 1교시에 농도는 487.2 ㎍/㎥, 4교시에 428.2 ㎍/㎥, 창문을 개방하지 않은 학급에서 1 교시에 농도는 1,283.5 ㎍/㎥, 4교시에 1,715.4 ㎍/㎥, 대조학교의 경우 2학급에서 각각 1교시에 농도는 240.9 ㎍/㎥, 150.8 ㎍/㎥, 4교시에 농도는 371.9 ㎍/㎥, 448.0 ㎍/㎥였다. 부호숫자 짝짓기의 평균반응시간은 대조학급이 1교시에 2,585 msec에서 4교시에 2,459 msec으로 감소한 반면 신축학교의 창문을 의도적으로 개방한 학급은 2,343 msec에서 2,510 msec으로, 평소대로 수업한 학급은 2,340 msec에서 2,563 msec으로 반응시간이 각각 167 msec, 223 msec 증가하였다. 1교시의 부호숫자 짝짓기 의 반응시간을 100%로 했을 때 창문을 의도적으로 개방 한 학급은 4교시에 5.9% 증가하였고(p<0.01), 평소대로 수업한 학급은8.3% 증가하였다(p<0.01). 반면에 대조학급은 4교시에 5.0% 감소하였다(p<0.01). 결론: 향후 학교의 실내공기질의 안전성 평가는 샘플링 된 공기의 오염수준의 측정뿐만 아니라 학생들의 건강영향도 함께 고려되는 것이 바람직할 것이다. Objectives: Most studies on the indoor air quality of newly built schools have focused only on the number of schools exceeding a standard or on subjective symptoms, such as sneezing, irritated eyes and an irritated nose. However, there has been no report on how poor indoor air quality affects children's academic performance. This study evaluated the effects of indoor air quality on the neurobehavioral performance of elementary school children. Methods: This study measured the indoor air pollutants and compared children's performance, using a computerized neurobehavioral test, between a newly built and a 12-year-old school. In addition, a questionnaire was administered regarding the indoor air quality related symptoms of children in a newly built and a 12-year-old school. Results: In the newly built school, the formaldehyde levels in the 1^(st) and 4^(th) classes with an open window were 34.6 and 27.3 μg/㎥, respectively. The levels in the 1^(st) and 4^(th) classes with the window closed were 80.2 and 127.1 μg/㎥, respectively. In the 12-year-old school, the formaldehyde levels in 1^(st) and 4^(th) class with open and closed window were 12.6 and 7.5 μg/㎥, and 19.8 and 25.2 μg/㎥, respectively. With respect to total volatile organic compounds, the levels in the 1^(st) and 4^(th) classes with the window open in the newly built school were 87.2 and 428.2 μg/㎥, respectively. In the 1^(st) and 4^(th) classes with the window closed, the total volatile organic compounds were 1,283.5 and 1,715.4 μg/㎥, respectively. In the 12-year-old school, the total volatile organic compounds were 240.9 and 150.8 μg/㎥, and 371.9 and 448.0 μg/㎥ in the 1^(st) and 4^(th) classes, respectively. In the case of a newly built school, the performance of the students in the neurobehavioral test in the 4^(th) class with the window closed was 8.3% lower than for those in the 1^(st) class with the window closed, but was only 5.9% lower when the windows were open. There was a statistical significant difference in the total numbers of symptoms between the two schools. Conclusions: These results suggest a proper evaluation of the indoor air quality within schools is required into both the level of contamination and the health effects of these contaminants toward children.

VDT작업별 정신사회적 스트레스와 근골격계 장애에 관한 연구

백남종,강종두,주영수,배인근,권호장,박종만,조수헌,김돈규,김재용,최홍렬 大韓産業醫學會 1998 대한직업환경의학회지 Vol.10 No.4

It has been hypothesized that jobs that have both high psychological demands and low decision latitude("job strain") can lead to musculoskeletal disorder. The objective of this study was to test whether job strain was correlated with the presence of work-related musculoskeletal disorder, especially myofascial pain syndrome(MPS). Information on demographic factors, confounders such as household load and taking care of children or not, and scores for decision latitude, job demand, and social support was obtained by self-administered questionnaire, which had been developed in Korean language, by adopting NIOSH instrument and Extended Karasek Model(16 items). All subjects were also examined by rehabilitation medicine specialists for musculoskeletal disorders. Subjects(n=370) could be categorized into 4 groups, these were, housewives(n=89), shipyard CAD workers(n=89), general female workers(n=79; nurses, insurance counselors, public officials, clerks, etc), and telephone directory assistance operators(n=113). Results from univariate analyses indicated that all demographic factors, all confounders, scores for decision latitude and social support were not associated with the risk of musculoskeletal disorder. However, score for job demand was higher in musculoskeletal disorder cases than others. In subgroup analysis, this association was convinced again, in telephone directory assistance operators. Job strain model showed that the group of telephone directory assistance operators was high-stain group, and OR of musculo skeletal disorder was 2.446(95% C. I. : 1.174, 5.096), when comparing this with the low strain group. In conclusion, job strain is a risk factor for work-related musculoskeletal disorder.

임상교정 교육용 멀티미디어 데이터베이스 웹서버 구축에 관한 연구

박재우,이종기,장영일,남동석,김명기,양원식,김태우,백승학 대한치과교정학회 2000 대한치과교정학회지 Vol.30 No.2

본 연구는 인터넷상의 가상공간에 다양한 자료로 구성된 환자 증례를 통해, 교정을 배우고자 하는 임상의나 학생들의 교육에 필요한 멀티미디어 데이터베이스를 구축하기 위한 것이다. 이러한 시스템을 구현하기 위해서는 이미지 자료의 처리, computer network를 통한 빠른 정보처리 기능, 진단 분석법의 개발, 데이터베이스의 구축 기술, 데이터베이스와 인터넷과의 연계, 증례를 통한 교육방법론과 같은 기술적 문제점들이 있다. 진단과 치료계획을 위한 분석은 모델 분석과 두부방사선 사진분석으로 나누어 제공하였다. 모델분석법은 arch length discrepancy와 Bolton tooth ratio를 환자 정보란에 제공하였다. 두부방사선 사진분석은 초진란에 제공하였다. Cephalometric analysis ver 2.0과 Power Ceph pro 3.3.5를 이용하여 Tweed, Steiner, Jarabak분석법 등을 제공하였으나, 본문에서는 주로 Kim's analysis와 몇 가지 계측치를 추가하여 설명하였고, 나머지는 tracing란에서 제시하였다. 또한 치료 종료 후나 보정란에서 치료 전, 후의 두부방사선 사진을 중첩해서 보여줌으로써 치료에 의한 효과를 직접 볼 수 있도록 하였다. The Objective of this research was to construct the multimedia database system that was necessary for the education of the practitioners and the students. To establish this system, there were technical problems as follows : 1) The processing of the images, 2) The rapid processing of the information with the computer network, 3) The development of diagnosis tools, 4) The technique to establish the database system, 5) The link between the database system and the internet, and 6) The method to educate through many cases. The analysis for diagnosis and treatment planning were provided as two parts : model analysis and cephalometric analysis. As a model analysis, arch length discrepancy and Bolton tooth ratio were provided for the part of patient information. Cephalometric analyses were provided in the part of initial diagnosis. The Cephalometric analysis ver 2.0 and the PowerCeph pro 3.3.5 were used to show Tweed, Steiner and Jarabak analysis. In the main part, Kim's analysis and some measurements were added. In the post-treatment or retention part, we show the superimposition of the cephalometrics with which you can find the effectiveness of the various orthodontic treatment. The address of this home page is "http://damis.snu.ac.kr/orthodontics"

한국인 직무 스트레스 측정도구의 개발 및 표준화

장세진,고상백,강동묵,김성아,강명근,이철갑,정진주,조정진,손미아,채창호,김정원,김정일,김형수,노상철,박재범,우종민,김수영,김정연,하미나,박정선,이경용,김형렬,공정옥,김인아,김정수,박준호,현숙정,손동국 大韓産業醫學會 2005 대한직업환경의학회지 Vol.17 No.4

Background and Purposes: Over the past three decades, numerous studies performed in Korea have reported that job stress is a determinant risk factor for chronic diseases and work disability. Every society has its own culture and occupational climate particular to their organizations, and hence experiences different occupational stress. An occupational stress measurement tool therefore needs to be developed to estimate it objectively. The purpose of this study is to develop and standardize the Korean Occupational Stress Scale (KOSS) which is considered to be unique and specific occupational stressors in Korean employees. Subjects and Methods: Data were obtained from the National Study for Development and Standardization of Occupational Stress (NSDSOS Project: 2002-2004). A total of 12,631 employees from a nationwide sample proportional to the Korean Standard Industrial Classification and the Korean Standard Occupational Classification were administered. The KOSS was developed for 2 years (2002-2004). In the first year, we collected 255 items from the most popular job stress measurement tools such as JCQ, ERI, NIOSH and OSI, and 44 items derived from the a qualitative study (depth interview). Forty-three items of KOSS, in the second year, were retained for use in the final version of the KOSS by using Delphi and factor analysis. Items were scored using conventional 1-2-3-4 Likert scores for the response categories. Results: We developed eight subscales by using factor analysis and validation process: physical environment (3 items), job demand (8 items), insufficient job control (5 items), interpersonal conflict (4 items), job insecurity (6 items), organizational system (7 items), lack of reward (6 items), and occupational climate (4 items). Together they explained 50.0% of total variance. Internal consistency alpha scores were ranged from 0.51 to 0.82. Twenty-four items of the short form of the KOSS (KOSS-SF) were also developed to estimate job stress in the work setting. Because the levels of the subscales of occupational stress were gender dependent, gender-specific standard norms for both the 43-item full version and the 24-item short form using a quartile for the subscales of KOSS were presented. Conclusion: The results of this study suggest that KOSS might be an appropriate measurement scale to estimate occupational stress of Korean employees. Further and more detailed study needs to be conducted to improve the validity of this scale.

WWW와 CORBA를 이용한 원격 의료진단시스템의 설계 및 구현

김성용,백종욱 위덕대학교 부설 전자기술연구소 1997 전자기술연구소 논문집 : 위덕대 Vol.1 No.1

본 논문에서는 WWW을 통하여 분산된 상이한 형태의 의료 영상들을 진단하고 용도에 따라 하이퍼미디어 기술을 이용하여 그룹별로 모델링하고, 저장하고, 검색하며, 지능적으로 표현할 수 있는 의료 정보 진단 시스템을 설계하고 구현하였다. 분산 객체 기술인 OMG의 CORBA를 사용하여 여러 병원에 분산되어 있는 의료 정보를 효율적으로 검색할 수 있도록 하였다. In this paper, we designed and implemented a medical diagnosis system which could store, retrieve, and present medical information using WWW(World Wide Web) technology. We make it possible that various forms of multimedia medical information on the distributed environments can be processed and presented on WWW browser. Using CORBA ORB technique, we make it possible that users can access transparently several remote medical database servers without knowing the actual addresses of database servers.

원형질체 융합에 의한 Aspergillus nidulans의 효소생산

백종현,김성준,양영기 조선대학교 기초과학연구소 1988 自然科學硏究 Vol.11 No.1

Aspergillus nidulans FGSC 473, 154 각각의 원형체질 형성 최적 조건을 구해서 FGSC 473(pyr^-,rib^-)과 FGSC 154(ade^-, met^-)의원형질체 융합을 통하여 얻은 hybrid 균주의 효소 활성도를 모균주의 효소활성도와 비교하였다. 각각의 실험결과는 다음과 같다. 원형질체 형성은 0.5% Novozym 234의 세포벽 분해효소를 0.6MKCl의 삼투압 안정체에 녹여 37℃에서 2시간 동안 반응시켰을 때 최적으로 나타났다. 원형질체 융합의 빈도는 0.22%~0.51%로 나타났다. 모균주와 hybrid균주간의 세포외 효소활성도를 비교해본 결과 hybrid 균주는 CMCase만이 모균주 보다 높았고 다른 효소들은 대체로 모균주보다 낮은 효소 활성도를 나타냈다. Formation of protoplasts from auxotrophes of Aspergillus nidulans FGSC 473 and 154 by Novozym 234, and their protoplast fusion between complementary mutants were investigated. From Aspergillus nidulans FGSC 473 (pyr^-, rib^-) and 154 (ade^-, met^-), hybrid strains (No. 1-13 Strain) were isolated by protoplast fusion. Enzyme activities of each of Aspergillus nidulans 473, 154 and hybrid strains (No. 1-13 strain) were investigated. The main results of the present study were as follows: Optmimal concentration and reaction time of the lytic enzyme for protoplasts were 5㎎/㎖ and 2hr respectively. As osmotic stabilizer for protoplast formation, 0.6M KCl was to be suitable. The fusion frequencies between complementary strains ranged from 0.22% to 0.51% . In Cellulaseand Xylanase system, the most of enzyme activities of hybrid strains were lower than those of parental stains, but only CMCase activities of hybrid strains were higher than those of parental strains.

전자식 선하증권의 운용에 따른 문제점에 관한 연구

김종락,백주홍 순천향대학교 사회과학연구소 2004 순천향 사회과학연구 Vol.9 No.2

Even if the purpose and functions of electronic Bills of Lading are the same as those of traditional B/L, the characteristics of media used for electronic Bills of Lading are different from those of traditional B/L in nature to have different technical operative features and legal effects. In this research, what the cause of the crisis of bills of lading is, what kinds of methods are introduced in electronic Bills of Lading to have the same functions as traditional B/L, and how the relationship between electronic Bills of Lading and related laws is formed were surveyed from commercial and legal aspects. To secure the function as document of title and the transferability of electronic Bills of Lading, various electronic Bills of Lading were studied and the Bolero system, succeeded in its commercialization mainly in Europe, was introduced. The Bolero electronic Bills of Lading operated on the Bolero system realized the essential operative functions of traditional B/L. Some of these are the receipt document of products, the evidence of the transportation contract of products and securities enabling the transfer of ownership etc. In addition, traditional transaction processes can be realized more rapidly without damaging the legal effectiveness in electronic Transactions. However, the Bolero is far from perfect. There are several problems in its practical usage. These problems can be reviewed from legal and practical aspects, respectively.

청양 지역에 분포하는 트라이아스기 후기의 조계리층에서 산출된 규화목 화석

김종헌,백승덕,이원국 공주대학교 과학교육연구소 2003 과학교육연구 Vol.34 No.-

충남 청양 지역에 분포하는 트라이아스기 후기의 조계리층에서 규화목 화석이 발견되었다. 규화목 화석의 산출은 남한에 분포하는 하부중생계 지층에서 최초의 기록이다. 분류학적 연구결과, 구과류에 속하는 Xenoxylon sp.와 Dadoxylon sp.의 2속 2종이 식별되었다. 본 논문에서 구과류 화석의 특징과 비교 및 그 의미에 대해서 고찰하였다. Petrified wood fossils are discovered from the Upper Triassic Jogyeri Formation distributed in the Cheongyang area. The occurrence of petrified fossils is the first record in the Lower Mesozoic strata distributed in South Korea. As a result of anatomical study of wood fossils, two specie belonging to two genera of Xenoxylon sp. and Dadoxylon sp. which belong to conifers, are recognized. This paper deals with the characteristics, comparison and significance of conifer wood fossils.

레슬링선수들의 체중감량에 따른 섭취열량과 신체조성의 변화

백영호,염종우,염원상,김세종,서혜림 부산대학교 체육과학연구소 2000 體育科學硏究所 論文集 Vol.16 No.-

This study was to find out calorie intake and body composition changes in accordance with weight loss of wrestlers. The wrestlers were divided three groups : no weight loss groups, 5∼7% weight loss group and over 7% weight loss group. And they were observed three : before weight loss, at measuring weight and before games. The following are the conclusion of this study: 1.The changes of calorie intake 1) Before weight loss, the percentage of the daily intake to the daily energy expenditure was 57.7% for the no weight loss group, 47.2% for the 5%∼7% weight loss group and 53.7% for the over 7% weight loss group. 2) The calorie intake of each group per meal: the no weight loss group intake 1,118kcal before weight loss, 760.9kcal before weight measurement and 1026.8kcal from after weight measurement to before games, and the 5∼7% weight loss group intake 955.4kcal before weight loss, 483.1kcal before weigh measurement and 880.4kcal from after weight measurement to before games, and the over 7% weight intake 959.3kcal before weight loss, 339.4kcal before weight measurement and 872.7kcal from after weight measurement to before games. 3) The ratios of calorie intake(protein : fat : glucose) : the no weight loss group showed 16.7∼35.1% : 20.8∼23.8% : 44.1∼60%, the 5∼7% weight loss group showed 15.5∼26.6% : 22.2∼23.7% : 50.6∼62.3% and the over 7% weight loss group showed 15.0∼30.2% : 19.6∼27,6% : 50.7∼59 4%. 2.The changes in body composition 1) After weight loss, weight and BMI changes in accordance with weight loss among groups showed meaningful differences in all circumstances : weight, before games versus at measuring weight and before weight loss versus before games.(p≤.001) 2) In the changes of total body water, protein, mineral, fat mass and %fat in accordance with weight loss among groups, when we compared those of before games with those of at measuring weight, we found that the over 7% weight loss group showed a meaningful increase over the no weight loss group.(p≤0.5) 3) In the changes within groups, though we found no meaningful difference, total body water, protein and mineral of before games were less than those of before weight loss, and fat mass and %fat of before games were higher than those of before weight loss. According to the results of this study, the wrestlers lost their weights intensively in three days before games by eating less and when lean body mass was restored, the level was lower than before weight loss. Therefore, these results intake the weakness of exercise capacity, long-term weight loss plan is more helpful than short-term weight loss, and for the enhancement of exercise capacity systematic plan and training of the weigh loss should be done.