삼릉(三稜) 추출물의 간세포 보호 및 미토콘드리아 보호 효과

서혜림,이주희,장미희,권영원,조일제,김광중,박숙자,김상찬,김영우,변성희,Seo, Hye-Lim,Lee, Ju-Hee,Jang, Mi-Hee,Kwon, Young-Won,Cho, Il-Je,Kim, Kwang-Joong,Park, Sook-Jahr,Kim, Sang-Chan,Kim, Young-Woo,Byun, Sung-Hui 대한한의학방제학회 2015 大韓韓醫學方劑學會誌 Vol.23 No.2

Objectives : Sparganii Rhizoma is frequently used in traditional herbal medicine for treatment of blood stasis, amenorrhea and functional dyspepsia and has been reported to exhibit anti-oxidant, anti-proliferation and anti-angiogenesis peoperties. In this study, we investigated the cytoprotective effect and underlying mechanism of Sparganii Rhizoma water extract (SRE) against oxidative stress-induced mitochondrial dysfunction and apoptosis in hepatocyte. Methods : To determine the effects of SRE on oxidative stress, we induced synergistic cytotoxicity by co-treatment of arachidonic acid (AA) and iron in the HepG2 cell, a human derived hepatocyte cell line. Results : Treatment of SRE increased relative cell viability and altered the expression levels of apoptosis-related proteins such as Bcl-xL, Bcl-2 and procaspase-3. And SRE also inhibited the mitochondrial dysfunction and excessive reactive oxygen species production induced by AA+iron. In addition, SRE activated of AMP-activated protein kinase (AMPK), a potential target for cytoprotection, by increasing the phosphorylation of AMPKα at Thr-172. Morever, SRE increased phosphorylation of acetyl-CoA carboxylase, a direct downstream target of AMPK. Conclusion : These results indicated that SRE has the ability to protect against oxidative stress-induced hepatocyte damage, which may be mediated with AMPK pathway.

Inhibition of WEE1 Potentiates Sensitivity to PARP Inhibitor in Biliary Tract Cancer

서혜림,남아롱,방주희,오경석,김재민,윤지선,김태용,오도연 대한암학회 2022 Cancer Research and Treatment Vol.54 No.2

Purpose Up to 20% of patients with biliary tract cancer (BTC) have alterations in DNA damage response (DDR) genes, including homologous recombination (HR) genes. Therefore, the DDR pathway could be a promising target for new drug development in BTC. We aim to investigate the anti-tumor effects using poly(ADP-ribose) polymerase (PARP) and WEE1 inhibitors in BTC. Materials and Methods We used 10 BTC cell lines to evaluate an anti-tumor effect of olaparib (a PARP inhibitor) and AZD1775 (a WEE1 inhibitor) in in vitro. Additionally, we established SNU869 xenograft model for in vivo experiments. Results In this study, we observed a modest anti-proliferative effect of olaparib. DNA double-strand break (DSB) and apoptosis were increased by olaparib in BTC cells. However, olaparib-induced DNA DSB was repaired through the HR pathway, and G2 arrest was induced to secure the time for repair. As AZD1775 typically regulates the G2/M checkpoint, we combined olaparib with AZD1775 to abrogate G2 arrest. We observed that AZD1775 downregulated p-CDK1, a G2/M cell cycle checkpoint protein, and induced early mitotic entry. AZD1775 also decreased CtIP and RAD51 expression and disrupted HR repair. In xenograft model, olaparib plus AZD1775 treatment reduced tumor growth more potently than did monotherapy with either drug. Conclusion This is the first study to suggest that olaparib combined with AZD1775 can induce synergistic anti-tumor effects against BTC. Combination therapy that blocks dual PARP and WEE1 has the potential to be further clinically developed for BTC patients. Purpose Up to 20% of patients with biliary tract cancer (BTC) have alterations in DNA damage response (DDR) genes, including homologous recombination (HR) genes. Therefore, the DDR pathway could be a promising target for new drug development in BTC. We aim to investigate the anti-tumor effects using poly(ADP-ribose) polymerase (PARP) and WEE1 inhibitors in BTC.Materials and Methods We used 10 BTC cell lines to evaluate an anti-tumor effect of olaparib (a PARP inhibitor) and AZD1775 (a WEE1 inhibitor) in in vitro. Additionally, we established SNU869 xenograft model for in vivo experiments.Results In this study, we observed a modest anti-proliferative effect of olaparib. DNA double-strand break (DSB) and apoptosis were increased by olaparib in BTC cells. However, olaparib-induced DNA DSB was repaired through the HR pathway, and G2 arrest was induced to secure the time for repair. As AZD1775 typically regulates the G2/M checkpoint, we combined olaparib with AZD1775 to abrogate G2 arrest. We observed that AZD1775 downregulated p-CDK1, a G2/M cell cycle checkpoint protein, and induced early mitotic entry. AZD1775 also decreased CtIP and RAD51 expression and disrupted HR repair. In xenograft model, olaparib plus AZD1775 treatment reduced tumor growth more potently than did monotherapy with either drug.Conclusion This is the first study to suggest that olaparib combined with AZD1775 can induce synergistic anti-tumor effects against BTC. Combination therapy that blocks dual PARP and WEE1 has the potential to be further clinically developed for BTC patients.

The effect of visual information on gait parameters with induced ankle muscle fatigue

서혜림,황진희,이수영 물리치료재활과학회 2017 Physical therapy rehabilitation science Vol.6 No.3

Objective: The purpose of this study was to investigate the effect of visual block with ankle joint fatigue on gait and dynamic balance ability. Design: Cross-sectional study. Methods: Thirty healthy young adults (men=15, women=15) between 22 to 25 years of age voluntarily participated in this study. All subjects performed the gait and dynamic balance test successively in two conditions: the visual block and the open eyes condition. Before the gait and dynamic balance test, muscle fatigue on the ankle joint was induced to all subjects by doing ankle dorsiflexion and plantarflexion alternately, and then gait parameters (step length, stride length, cadence, velocity, single limb support, and double limb support) were assessed by walking on the GAITRite system (CIR Systems Inc., USA). Subjects also performed the functional reach test (FRT) for assessment of dynamic balance. This study examined gait parameters and FRT scores in each visual block and open eyes condition. Results: The results showed that FRT scores with the visual block condition significantly decreased compared to without visual blocking (p<0.01). Step length, stride length, cadence, and velocity of gait parameters decreased significantly in the visual block condition (p<0.01) while there was no significant difference for single limb support. However, double limb support increased significantly in the visual block system (p<0.01). Conclusions: Therefore, blocking of visual information with muscle fatigue of the ankle joint can affect gait and balance ability of young adults and increase the risk of falls.

MICE산업 활성화와 지방정부의 지원정책 비교 분석

서혜림,박인권 한국공간환경학회 2014 한국공간환경학회 학술대회 논문집 Vol.2014 No.1

사전 질문에 대한 외적/내적 틀린 인출이 후속 학습에 미치는 영향

서혜림,남서현,박주용 한국인지및생물심리학회 2012 한국심리학회지 인지 및 생물 Vol.24 No.3

The pretesting effect refers to the enhancement of learning due to unsuccessful retrieval upon being asked a question that is not easily answered. However, the results of research on the effect of overt retrieval on learning, have not been consistent. Therefore, the present study sought to clarify such confusion. We examined whether memory enhancement is affected by the number of wrong answers generated by the examinees and by the duration of retrieval. Four experiments were carried out with college students as participants. In Experiments 1 and 2, we manipulated the number of unsuccessful retrievals to either 1 or 3, and observed that there was no difference in performance. In Experiments 3 and 4, participants were asked to think of possible answers without overt responses. The results showed that the performance was better for those who were asked to think of more answers. The present study also examined whether pretesting effect is found even after a week's duration. After a week, pretesting effect was observed in case of the covert retrieval group; however it did not last for the overt retrieval group. These results support the search set theory by Grimaladi and Karpicke (2012) which states that active exploration of related material promotes learning. The present study also suggests that overt retrieval brings about retrieval competition and interferes with the retrieval of correct responses, and thus disrupts learning. 정답을 맞히기 어려운 문제를 제시하여 틀린 인출을 유도하는 사전시험(pretesting) 연구들은, 틀린 인출이 학습을 촉진하는 결과를 보여주고 있다. 하지만 틀린 인출 중 오답을 산출하는 것이 학습에 어떠한 영향을 미치는지에 대하여 보고된 결과들은 일관되지 않다. 본 연구에서는 먼저 오답 개수와 탐색 시간의 체계적 변화에 따른 학습 효과 차이를 알아보았다. 실험 1, 2에서는 참가자의 오답 반응수를 1개와 3개로 다르게 변화시켰는데 오답 산출 증가에 따른 수행 차이가 나타나지 않았다. 그러나 참가자에게 내적 인출 즉, 오답을 산출하지 않고 사전 문제와 관련된 정보를 탐색만 하도록 지시한 실험 3, 4에서는 탐색을 많이 할수록 수행도 함께 증가하였다. 본 연구에서는 또한 기존 연구들보다 지연시간이 긴 경우에도 사전시험 효과가 나타나는지 알아보았는데, 내적 탐색만 하는 경우 학습 효과가 일주일 뒤에도 확인되었다. 그러나 외적으로 오답을 산출한 경우 즉시검사에서 나타났던 효과가 일주일 후 지연검사에서는 사라졌다. 이러한 결과들은 사전시험에서 관련된 정보를 능동적으로 탐색하는 것이 학습을 촉진한다는 탐색 집합 이론(Grimaldi & Karpick, 2012)을 지지하는 동시에, 오답을 직접 산출하면 지연검사에서 정답과 인출 경쟁을 일으켜 학습을 방해할 수 있음을 시사한다.

MICE산업 활성화와 지방정부의 지원정책 비교 분석

서혜림,박인권 대한지리학회 2014 대한지리학회 학술대회논문집 Vol.2014 No.6

텍토닉

서혜림 현대건축사 1998 월간 CONCEPT Vol.- No.3

인조 합성 테니스 스포츠 바닥재의 운동생리학적 분석

서혜림,박승범,서국웅,이재규,서혜진 釜山大學校 附設 體育科學硏究所 1999 體育科學硏究所 論文集 Vol.15 No.-

The purpose of this study was to investigate the changes of heart rate(HR), Ventileation, Carbon elimination, Oxygen take, Oxygen uptake per weight, Respiratory Exchange ratio and Breath frequency. To do this, we selected three Synthetic tennis playing surface(A:Synpave Rebound Ace Sample, B:Synpave Rebound Classic Sample, C:Synpave Spring Sample) by using of Austrilian Tennis Open. The result of this study is as follows 1. In the changes of heart rate(HR, beats/min), it is not significantly in a stastics. Tennis playing surface sample A was shown a little bit higher than sample C, sample C was shown a little bit higher than sample B, 2. In the changes of Ventileation(VE, 1/min), it is not significantly in a stastics. Tennis playing surface sample B was shown a little bit higher than sample A·C, sample C was shown a little bit higher than sample A, 3. In the changes of Carbon elimination (Vco2, ml/min), it is not significantly in a stastics. Tennis playing surface sample A was shown a little bit higher than sample B·C, sample B was shown a little bit higher than sample C, 4. In the changes of Oxygen take (Vo2, ml/min), it is not significantly in a stastics. Tennis playing surface sample A was shown a little bit higher than sample B·C, sample B was shown a little bit higher than sample C, 5. In the changes of Oxygen uptake per weight(Vo2/wt, ml/kg/min), it is not significantly in a stastics. Tennis playing surface sample A was shown a little bit higher than sample B·C, sample B was shown a little bit higher than sample C, 6. In the changes of Respiratory Exchange ratio(RER, Vco2/Vo2), it is not significantly in a stastics. Tennis playing surface sample A was shown a little bit higher than sample B·C, sample C was shown a little bit higher than sample B, 7. In the changes of Breath frequency (BF, fre/min), it is not significantly in a stastics. Tennis playing surface sample B was shown a little bit higher than sample A·C.

서울시 도시문화시설의 입지특성에 관한 연구

서혜림(Hye Rim Seo),김근영(Geunyoung Kim) 한국지역개발학회 2010 한국지역개발학회 학술대회 Vol.2010 No.11

Urban cultural facilities are the facilities for providing facilities, equipments, data and documents, information, and education services for cultural activities of urban residents. Culture is defined as the complex and comprehensive life-style composed of regulations, customs, and activities that have learned and educated by a society's members. World cities in 21st century are creating cultural and historical images of cities using urban cultural resources including arts, historical heritages, and entertainment resources. They also improve competitiveness of urban industries by developing new creative businesses with high added values in fields of design, entertainment, and tourism industries by connecting urban cultural activities with cultural industry strategies. The demand of urban cultural activities is in increasing trend when Koreans national income is recently close to 20,000 U.S. dollars per capita. Central and local governments are building cultural clusters and cultural streets in order to satisfy cultural needs of city residents. However, there has been a lack of understanding to public administrators and urban planners in existing urban culture and cultural facilities. The objective of this research is to analyze location characteristics of urban cultural facilities using statistical analysis methods and GIS techniques in the Seoul metropolitan city.

Disciplinary Session7 / Sport Physiology ( Baekhap Rm ) : 교육수준에 따른 노인들의 자가건강관리형태 , 일상생활수행능력 , 신체적 자기효능감에 관한 연구

서혜림(Hea Lim Seo),백영호(Yeong Ho Baek),이의철(Eui Chul Lee) 한국체육학회 1999 New Millennium and Sport Vision PROCEEDINGS1 Vol.- No.-