단백질 S 결핍증에 의한 장간막정맥혈전증 치험 1예

김용신,박창영,손정일,김병익,서원태,김명숙,전우규,정을순,김흥대 대한소화기학회 1999 대한소화기학회지 Vol.34 No.6

Mesenteric venous thrombosis is rare but clinically important because it can cause ischemia or infarction in the small intestine. Unless it is diagnosed in time, the patient die eventually. However, the diagnosis is difficult because patients often present with nonspecific abdominal symptoms. Furthermore, if it occurs in young individuals without any known predisposing factor, the diagnosis may become more difficult. Recently, the development of modern imaging techniques (particularly ultrasonography, duplex scanning, and computed tomography) have enabled early recognition of this disease. We report a case of mesenteric venous thrombosis and deep vein thrombosis of left lower extremity. A patient was admitted to our hospital due to continuous, dull abdominal pain. The simple abdomen examination showed abnormal gas pattern probably due to small bowel edema. Abdominal computerized tomography revealed mesenteric venous thrombosis with bowel wall thickenings. Bowel resection was performed and the patient was recovered soon. Mesenteric venous thrombosis was known to be secondary to protein S deficiency later.

복발효 감식초의 품질

김미경,김미정,김소연,정대성,정용진,김순동 대구효성가톨릭대학교 식품과학연구소 1994 식품과학지 Vol.6 No.-

고품질의 감식초를 농가에서 자가생산하기 위한 기초적 자료를 마련할 목적으로 자연발효시킨 경우와 복발효시킨 경우의 품질을 비교하였다. 그 결과 복발효시킨 식초는 자연발효 식초에 비하여 초산농도가 1/2인 3%정도였다. 그리고 HPLC 법으로 측정한 유리당의 총함량은 자연발효 식초는 6%, 복발효시킨 것은 5%였다. 감식초내에 함유된 유기산류로는 oxalic acid, malic acid, lactic acid, citric acid, succinic acid, glutaric acid 등 이었으며 자연발효시킨 경우가 전반적으로 함량이 높았다. 특히 자연 발효시킨 경우 lactic acid함량이 높았다. 그러나 감과실의 flavour와 과실의 고유색상과 맛은 복발효시킨 경우가 강하게 나타났다. To prepare the basic information for the fermentation of high quality persimmon vinegar in the farm, the quality of the complex fermented persimmon vinegar(CFPV) was compared to that of naturally fermented persimmon vinegar(NFPV). The titratable acidity of the CFPV was 3% which was the half concentration of the NFPV. The content of total free sugar by HPLC was 6% in CFPV and NFPV was 5%. Organic acid compositions in persimmon vinegar were oxalic acid, malic acid, lactic acid, citric acid, succinic acid and glutaric acid in addition to acetic acid. Total content of the organic acids in NFPV was higer than that in CFPV. Especially, the content of latic acid in NFPV was very higher than that of CFPV. The flavour, color and taste of persimmon fruit itself in CFPV were stronger than those in NFPV.

베타-글루쿠로니다제에 의한 황련ㆍ황금 공침물의 가수분해 및 생체이용률 증가

김미정,김남순,김영일,김대근,양재헌 한국약제학회 2003 Journal of Pharmaceutical Investigation Vol.33 No.2

During the preparation of decoction from the mixture of Coptidis Rhizoma and Scutellariae Radix, insoluble copreciptate was formed. The coprecipitated product(COP) was composed of berberine and baicalin which was the active ingredient of Coptidis Rhizoma and Scutellariae Radix, respectively. COP was slightly soluble in water and could not be well absorbed after oral administration. This poor bioavailibility might be associated with its poor aqueous solubility. With the purpose of increasing the solubility and bioavailibility of COP, hydrolysis of COP by β-glucuronidase was carried out. Hydrolyzed products (HOP) of COP were identified and assayed for active ingredients. The partition coefficient study, in situ absorption test, and pharmacokinetic study after oral administration were also performed. COP was found to be consisted of berberine and baicalin with molecular ratio or 1 to 1. This compound was hydrolyzed to berberine and baicalin by β-glucuronidase. The rate of hydrolysis was higher at higher temperature up to 50℃ and higher concentration of β-glucuronidase up to 2500 unit under our experimental conditions. Baicalein, which is more liphophilic than baicalin, showed greater absorption in small intestine than baicalin did. The plasma concentrations of berberine and baicalein after oral administration of HOP were significantly higher than those of COP. The possible mechanism of increased bioavailibility of berberine and baicalein could be the hydrolysis of COP by β-glucuronidase. On the basis of the above results, it might be said that HOP should be a suitable preparation for increasing the bioavailibility of Coptidis Rhizoma and Scutellariae Radix.

8년간(1992~1999) 산업안전보건연구원에 의뢰된 직업병 심의 사례 분석

강성규,김규상,김양호,최정근,안연순,진영우,최병순,양정선,김은아,채창호,최용휴,김대성,박정선,정호근 대한산업의학회 2000 대한직업환경의학회지 Vol.12 No.2

Objectives : Pneumoconisis and noise-induced hearing loss (NIHL) have been reported as main occupational diseases by the Special Health Examination. The Industrial Accident Compensation Insurance has reported various work-related diseases, however, these two diseases occupied almost a half of compensated cases. Therefore, it was not well known about the status of occupational diseases other than pneumoconiosis, NIHL, and cardio-cerebrovascular accident (CVA). This study was conducted to analyze claimed cases as an occupational disease, that was requested to the Korea Industrial Safety and Health Agency (KOSHA). Methods : The local office of the Korea Labor Welfare Corporation (KLWC) has asked the KOSHA for confirmation of claimed cases as an occupational disease. We analyzed 379 cases requested from KLWC, the Ministry of Labor, employers, unions and occupational health agencies from 1992 to 1999. Results : Male was 80.7 % of the requested cases. Their mean age was 42 years old and 75.5 % of them were more than 35 years old. The requested cases were increased rapidly from 25 cases in 1992 to 108 cases in 1999 and the accept rate was 50.7 %. The majority of requested cases were respiratory diseases(22.4%), cancers(18.5%), Neuropsychiatric problems(14.5%), and musculoskeletal problems(13.5%). The accept rate was high in reproductive, respiratory, musculoskeletal and digestive disorders and low in neuropsychiatric, renal and otologic problems and occupational cancers. 73.6% of them were caused by chemical agents, especially 28.5 % were by organic solvents. 67% of them were clinically confirmed at university hospitals. A half of the cases were from KyongIn area, even the request came from the whole country. Conclusions : A claim was common in workers whose age was over 35 years old and exposure history was over 10 years. The respiratory diseases and neuropsychiatric disorders were still main problems in occupational health and occupational cancers was increasing even though its accept rate was not high yet.

우리나라 長壽者의 生活 및 意識調査에 관한 硏究 - Ⅴ. 過去의 健康疾病 및 苦痛에 대한 態度 -

최진호(Jin-Ho Choi),변재형(Jae-Hyeung Pyeun),임채환(Chae-Hwan Rhim),양종순(Jong-Soon Yang),김수현(Soo-Hyun Kim),김정한(Jeung-Han Kim),이병호(Byeong-Ho Lee),우순임(Soon-Im Woo),최선남(Sun-Nam Choe),변대석(Dae-Seok Byun),김무남(Mu-Nam 군산대학교 수산과학연구소 1994 군산대학교 수산과학연구소 연구논문집 Vol.1 No.-

The present study was designed to be link a part of the research on the investigation on daily life and consciousness of the longevous people in Korea, and to investigate the health conditions, and the attitude on disease and suffering of longevous people in the past. 1. By the health conditions of longevous people between 40 and 60 years, ""very good"" showed the highest figure of 71.0%, followed by ""ordinary"" (21.6%). Consequently it is found that 92.6% of total longevous people surveyed maintained the good health conditions above ordinary. 2. Of the experiences of disease and/or injury after 60 years of longevous people, ""never"" showed the highest figure of 74.7%, while ""had"" showed the only 22.6% of total longevous people surveyed. 3. Of the details of disease and/or injury after 60 years of longevous people. ""digestive disease"" showed the highest figure of 32.6%. followed by ""apoplexia cerebri & hypertensions""(14.0%) and ""heart disease""(14.0%), and followed by ""respiratory disease""(12.3%). 4. Of the experiences of suffering between 40 and 60 years of longevous people, ""never"" showed 53.7% of total longevous people surveyed, while the frequency of suffering decreased in the order of ""once""(22.7%), ""2 times""(15.8%) and ""3 times""(4.7%). By the details of suffering between 40 and 60 years of longevous people. ""family life"" showed the highest figure of 85.1%, followed by ""business""(10.1%). 5. By the attitude on suffering of longevous people, ""tried to forget soon"" showed the highest figure of 54.2%, followed by ""continuous worried""(23.3%), and followed by ""not worried""(7.2%).

Methexymethyl sulfide를 이용한 합성연구

김정순,고영관,김대광,윤세중 공주대학교 기초과학연구소 1992 論文集 Vol.30 No.1

For the preparation of broad range of substituted aryl derivatives from the bromophenyl methoxymethyl sulfide and the bromobenzyl methoxymethyl sulfide, various functional groups such as alkyl, ester and haloketone could be easily introduced in the aromatic methoxymethyl sulfides by treatment with various electrophiles. Substituted methoxymethyl phenyl sulfides were converted to the arenesulfonyl chlorides with chlorine in water-dichloromethane at 5∼10℃. And substituted benzyl methoxymethyl sulfides were also converted to the arylmethanesulfonyl chlorides with N-chlorosuccinimide in aqueous acetic acid, at 5∼10℃.

유럽共同體法上의 無效訴訟(Nichtigkeitsklage)에 관한 硏究

金大淳 전북대학교 법학연구소 1986 法學硏究 Vol.13 No.-

Abstract This paper deals with an annulment action (in German terminology Nichtigkeitsklage) in the EC law, which is laid down by Article 173 EEC, Article 146 Euratom, and Article 33 ECSC respectively. Article 146 Euratom is in entirely identical terms with Article 173 EEC. In this paper, mainly the annulment action of the EEC is discussed, with an occasional mention of that of the ECSC. The aim of an annulment action is to have the European Court declare void an invalid act of the Council or the Commission. Article 173 EEC provides that 'the Court of Justice shall review the legality of acts of the Council and the Commission other than recommendations or opinions', while Article 33 ECSC states that 'the Court shall have jurisdiction... to have decisions or recommendations of the High Authority declared void.... The two provisions form a contrast in enumerating the reviewable acts. In the former case, all the legal acts , especially including the so-called 'acts suigeneris' and possibly an international agreement between the European Community and a third country, can be the object of an annulment action. Under the latter system, on the contrary, only the legal acts mentioned there, that is the decision or the recommendation of the High Authority can be reviewed by the European Court. Therefore it is fairly safe to say that the scope of the jurisdiction ratione materiae of the European Court is much wider under the EEC(and Euratom) system in comparison with that under the ECSC system. Who can institute annulment proceedings? The applicants are divided into two categories, privileged and non-privileged applicants. The privileged applicants are the Council, the Commission and the Member States. The Council may institute proceedings against the legal acts of the Commission, the Commission against the legal acts of the Council, and any Member State against the legal acts of either of the two. The locus standi of the privileged applicants is not limited by any special requirements. The reason is that every act of the Council or the Commission concerns them. The non-privileged applicants are natural persons and legal persons. Article 173 EEC limits the locus standi' of the non-privileged applicants as follows : Any natural or legal person may... institute proceedings against a decision addressed to that person or against a decision which, although in the form of a regulation or a decision addressed to another person, is of direct and individual concern to the former. Two points need mentioning in connection with the above provision. First, the non-privileged applicants may bring proceedings against only one category of legal acts, a 'decision'. The above provision predicts three kinds of decisions : a decision addressed to the applicants, a decision in the formed a regulation, and a decision addressed to another person. Then, what is a decision ? How can it be defined at all ? In determining this problem, the European Court dose not depend on the formal designation of a legal act. Therefore the Court ruled in one case that a letter of the Commission addressed to the applicant is a decision. In short, the term 'decision' means an act not in the formal but in the material sense. The reason why a disguised decision in the form of a regulation has been inserted into Article 173 is to prevent the Council or the Commission from attempting to blockade the institution by a natural or a legal person of an annulment action simply by labelling its act 'regulation'. Second, in order to bring an annulment action, the non-privileged applicants must prove that the decision (in the material sense) at issue is of direct and individual concern to them. The meaning of direct and individual concern has not been defined so successfully and consistently by the European Court. The grounds which may be the object of judicial review are four : lack of competence, infringement of an essential procedural requirement, infringement of the EC Treaties or any rule of law relating to their application, and the misuse of powers. These four grounds are said to have come from the French administrative law. This does not, however, mean that their meanings should be determind according to their source. These terms are now community legal ones, and therefore their meanings should be fixed on the Community level. It is generally observed by the European writers that the formation of grounds is not so satisfactory. For the third ground is comprehensive enough to cover all the other three. In principle, the invalid acts of the Commission or the Council are voidable, not void. In the EC law, most legal acts are deemed to be effective until they have been finally declared void by the European Court. The situation is rare that the legal acts of the Commity are absolutely invalid or non-existent from the beginning. The final ruling by the European Court annuls the act at issue retroactively and affects everybody, erga omnes. The writer comes to the idea that the annulment action system under the EC legal order serves satisfactorily to promote the integration of the Western European peoples. But it is submitted that the special requirement adding to the difficulties in proving the invalidity of an act, that is the direct and individual concern, needs modifying which only the non-privileged applicants encounter before the Court.

혈청 Fructosamine에 대한 임상적 유용성에 관한 연구

김철,김용태,노상현,홍순표,김미송,장숙진,박희완,문대수 朝鮮大學校 附設 醫學硏究所 1991 The Medical Journal of Chosun University Vol.16 No.1

Diabetes mellitus is a universal health problem and may occur at any age. The traditional biochemical measurements for initially detection patients with diabetes mellitus are random estimations of blood and urine glucose concentrations. Despite their common use both tests are fairly non-specific, being influenced by wide variety of condition. Measurement of the HbA_(1c) in a random blood specimen is a more simple screening test for diabetes, and widely used as an index of diabetic control. But unfortunately, it has difficulties with methodology. Fructosamine has been described as a new parameter for the estimation of glycosylated protein, It is based on the reduction potential of ketoamines in an alkaline medium and measures colorimethcally the concentration of all glycosylated semm proteins belonging to the class of fructosamine. It reflects the time-integrated glucose concentration over the previous 1 to 3 weeks and can be used for the follow up of diabetes mellitus. We measured serum fructosamine concentration and compared with any other items. The results are summehzed as follows : 1. Within-run and between-run imprecision were assessed by 20 analysis of 3 differential fructosamine concentration in control sera. The respective coefficients of variation were 2.9%, 6.6% in low concentration, 2.2%, 5.1% in medium concentration and 3.3%, 4.7% in high concentration. 2. The serum fructosamine concentration were 2.26±0.21 mmol/L in normal control subjects(n=29) and 3.35±0.65 mmol/L (n=62) in patients with diabetes mellitus (p<0.001). 3. In diabetic patients (n=62), the serum fructosamine concentrations correlated less significantly with glucose (Y(fructosamine) = 0.01×(glucose)+2.52, r=0.54, P<0.001) and more significantly with HbA_(1c) (Y(fructosamine) = 0.22×(HbA_(1c))+1.71 , r=0.63, P<0.001). 4. The concentrations of fructosamine in serum and EDTA plasma were not significently diferent (P<0.01). So, Plasma is as well suited to fructosamine determinations as serum(Y(serum) = 0.95×(Plasma)+0.25, r=0.96). 5. No interference was observed with lipemic sera (cholesterol) in detection of serum fructosamine(P=0.393).

성장에 따른 백서 절치유두의 신경 분포 변화

김영진,남순현,김현정,이재문,구대학 大韓小兒齒科學會 1996 大韓小兒齒科學會誌 Vol.23 No.1

출생후 성장에 따른 절치 유두의 신경분포 및 변화를 관찰하기 위하여 생후 1, 5, 10, 15, 20, 25, 35일 된 백서와 완전히 성장한 백서를 대상으로 calcitonin gene related peptide (CGRP)를 이용한 면역조직 화학적 방법으로 관찰하여 다음과 같은 결과를 얻었다. 생후 1일군에서는 염주알 모양의 굵은 신경섬유가 성긴 결합조직에 나타났다. 5일군에서는 이러한 신경섬유의 밀도와 분지가 증가하여 상피하층으로 더욱 가까이 연장되었고 여러개의 varicosities도 관찰되었다. 10일군부터 신경섬유의 분지 및 밀도가 증가하였고 상피하층에서 신경총을 관찰할 수 있었으며 상피내 신경섬유의 진입을 관찰할 수 있었다. 20일군에서는 증가된 상피층내 신경섬유를 관찰할 수 있었으나 25일군에서부터 전반적인 신경섬유의 분포 및 밀도가 많이 감소되었으며 성장이 완료된 군에서는 chemosensory corpuscle 지역을 제외하고는 신경섬유를 거의 관찰할 수 없었다. The purpose of this study was to investigate the distribution of nerves in the incisive papilla of rat by each developing stage. The distribution on nerves in the incisive papilla was investigated by means of immunohistochemistry using an antibody against calcitonin gene related peptide (CGRP). The results were as follows : At 1 day after birth, CGRP-immunoreactive(IR) nerve fibers first appeared in loose connective tissue without forming an apparent terminal structure. At 5 days, the CGRP-IR nerve fibers increased in density and extended more to subepithelium. CGRP-IR nerve fiber began to penetrate into the epithelium at 10 days and reached maximum at 20 days. At 25 days, intraepithelial nerve fibers were not found, and the distribution and density of nerve fiber began to decrease. In adult rat, the nerve fiber almost could not be found in dome region except in chemosensory corpuscle region.

Pb_(1-X)SN_XTe 단결정 성장과 특성 연구

김대중,이현순 圓光大學校 基礎自然科學硏究所 2000 基礎科學硏究誌 Vol.18 No.1

HWP(hot wall epitaxy)법으로 BaF_2기판 위에 Pb_(1-x)Sn_nTe단결정 박막을 성장시켰다. source온도는 510~530℃로 변화시키면서 결정을 성장시켰다. 성장된 박막의 결정성은 XRD(X-ray diffraction)와 DCXRD(double crystal X-ray diffraction)를 사용하여 조사한 결과 격자상수는 6.462~6.372Å이었고, XRD 피크의 반치폭(FWHM)은 560~900arcsec로 나타났다. Pb_(1-x)Sn_nTe박막의 특성을 알아보기 위하여 spectroscopic ellipsometry(SE) technique을 사용하여 복소유전율(ε) 및 복소굴절율(n^*), 흡수계수(α), 반사율(R)의 값을 구했다. Pb_(1-x)Sn_nTe films were grown on BaF_2 substrates by the hot wall epitaxy(HWE) technique. The single crystals have grown controlling that source temperature from 510℃ to 530℃ and substrate temperature from 300℃ to 340℃, respectively. The structural properties of thin films were measured using the X-ray diffraction(XRD) and double crystal x-ray diffraction(DCXRD).The lattice constant of thin films were from 6.462Å to 6.372Å.The full width at half maximum(FWHM)were from 560 arcsec to 900 arcsec. The optical constants, such as the complex dielectric funtion(ε), complex refrative index(n^*), absorption coefficient(α) and normal incidence reflectivity(R), of the Pb_(1-x)Sn_nTe films hve been determined using spectroscopic ellipsometry(SE) technique