Methylation Status and Expression of E-cadherin in Oral Squamous Cell Carcinomas Compared to Benign Oral Epithelial Lesions

Son, Hyun-Jin,Chu, Jung-Youb,Cho, Eui-Sic,Lee, Dong-Geun,Min, Myung-Gee,Lee, Suk-Keun,Cho, Nam-Pyo The Korean Academy of Oral Biology 2006 International Journal of Oral Biology Vol.31 No.2

Expression of invasion/metastasis suppressor, E-cadherin, is reduced in many types of human carcinomas. Although somatic and germline utations in the CDH1, which encodes the human E-cadherin, have frequently been reported in cases with diffuse gastric and lobular breast ancers, irreversible genetic inactivations are rare in other human carcinomas. Recently, it has been well documented that some genes in human cancers may be inactivated by altered CpG methylation. Herein, we determined the expression and methylation status of E-cadherin in oral squamous cell carcinoma (SCC) by immunohistochemistry and methylation-specific PCR. The expression of E-cadherin was significantly higher in the well-differentiated oral SCCs than the moderately or poorly differentiated ones. None of eight tested benign pithelial hyperplasias showed aberrant methylation, whereas five of 12 oral squamous cell carcinomas showed aberrant methylation. When we compared E-cadherin expression with methylation status, oral SCCs with normal methylation showed a higher expression of E-cadherin than those with methylation. These findings suggest that aberrant CpG methylation of CDH1 promoter region is closely associated with transcriptional inactivation and might be involved in tumor progression of the oral mucosa.

Inoue Balloon을 이용한 PMV의 장·단기 결과 분석

진은선,이연아,전숙,김현숙,정승묵,박상선,최락경,임달수,홍석근,황흥곤 대한순환기학회 2003 Korean Circulation Journal Vol.33 No.11

유방암의 위와 대장전이

유현아,김은영,서민지,정은,조민정,오현진,장지혜,박지찬,이정의,박석영 이화여자대학교 의과학연구소 2014 EMJ (Ewha medical journal) Vol.37 No.2

Gastric metastasis from breast cancer is rare and only six cases have been reported in Korea. Colon metastasis is more rare than gastric metastasis. We report a 63-year-old woman with gastric and colon metastases of invasive lobular carcinoma of breast. She was diagnosed as right breast cancer, received right modified radical mastectomy 10 years ago and has been treated with chemotherapy and hormone therapy. Investigating for melena and a small caliber of stool, we found gastric and colon metastases. The diagnosis of metastatic breast cancer was made through gross pathologic and immunohistochemistry staining. We report a case with gastric and colon metastases from breast cancer and a review of the associated six case reports in Korea.

성장기 아동에서 Cervical Headger 사용시 골격적 변화 양상에 대한 연구

현하영,이진우,차경석 대한치과교정학회 1996 대한치과교정학회지 Vol.26 No.5

성장기 아동의 Class II 부정교합에서 과도한 성장의 억제, 미약한 성장의 촉진으로 성장 시기에 적절히 치료하는 것이 가장 효과적이며 좋은 치료 방법이다. 이러한 방법 중 가장 적절히 쓰이는 장치 중 Cervical headgear의 사용을 들수 있으며 악골에 효과적으로 적용된다. 그러나 장치의 부작용이라 할 수 있는 수직적 성장의 과잉을 볼 수 있는데 이에 대해 많은 서학들의 연구가 시행되어 왔다. 본 연구는 Cervial headgear의 상하악골에 대한 효과를 분석하고 Lower facial height를 기준으로 증가군(>0)과 증가하지 않은 군 ( ≤0)으로 나누어 골격적 특성을 알아보기 위해 단국대학교 부속 치과병원에 내원한 성장기 아동의 II급 부정교합환자 25명을 대상으로 cervical headgear를 사용한 결과 다음과 같이 다소의 지견을 얻었기에 보고하는 바이다. 1. 상악골의 전방 성분이 억제되었고, 구개 평면의 전방이 하방으로 tipping되었으며 상악 제 1대구치의 후방 이동이 있었다. 2. 하악골의 두개저에 대한 상대적인 전방 이동이 있었으며 상악에 대한 하악 제1대구치의 상대적인 전방 이동이 있었고 하악골의 alveolar growth에 의한 수직적인 증가가 있었다. 3. 전안면 고경과 후안면 고경이 각기 유의하게 증가했으나 안면 고경 비율에는 유의성이 없었다. 4. Lower facial height가 증가한 group이 증가하지 않은 group보다 ramus의 길이가 짧고 palatal plane angle이 더 작았으며 상악 제 1대구치의 후방 이동량이 더 많았다. This study was to investigate the horizontal & vertical bone change pattern when using cervical headgear in Class II malocclusion of growing children and compared the skeletal features between the group with increased lower facial height and the group without increase in lower facial height. The results are as follows ; 1. Forward growth of maxilla was inhibited, downward tipping of anterior palatal plane could be seen and distal movement of maxillary first molar was observed. 2. There was relative forward movement of Mandible against the Maxillary cranial base, and relative forward movement of mandibular 1st molar against the Maxilla and vertical increase due to alveolar growth of Mandible. 3. There was significant increase in anterior and posterior facial heights but the ratio of facial height showed no significant difference. 4. The group with increased lower facial height has shorter ramus length, than the smaller palatal plane angle, and more distal movement of Maxillary 1st molar than the group without increase Ha-young Hyun.

콘크리트 構造物의 混合모드 龜裂傳播모델(第1報)

陳致燮,張熙碩,崔顯泰,嚴長燮 부산대학교 공과대학 1989 硏究報告 Vol.38 No.-

This paper attempts to extract the crack tip stress intensity factors and to make a crack propagation model under mixed mode, from the finite element analysis of the concrete structures. In finite element analysis, all elements are initially assumed to be conventional quadratic isoparametric elements and are replaced by 6 node triangular singularity elements in the crack tip zone only. The stress intensity factors and the angle of propagation are carried out by displacement correlation technique and maximum circumferential tensile stress theory respectively. At the first part in a series of two papers, some numerical examples about the concrete SENB and DISK are presented to illustrate the practical effectiveness of the technique.

피로와 의료이용, 업무상 사고 및 질병결근

장세진,고상백,강동묵,김성아,정진주,이철갑,강명근,현숙정,조정진,차봉석,박종구 大韓産業醫學會 2005 대한직업환경의학회지 Vol.17 No.4

Objectives: This study examined the relationship of fatigue to medical utilization, occupational accident and sickness absence. Subjects and Methods: Data were obtained from the National Study for Development and Standardization of Occupational Stress (NSDSOS Project, 2002~2004). A total of 30,146 employees categorized within the Korean Standard Industrial Classification and the Korean Standard Occupational Classification were recruited. A structured-questionnaire was used to assess the participants' sociodemo-graphics, job-related factors, health-related behaviors, personality trait, self=perceived fatigue (MFS) and the frequency of medical utilization, occupational accident and sickness absence. Data on medical utilization and occupational accident were confirmed from company health records. Chi-square test and logistic regression analysis were used to elucidate the relationship of self-perceived fatigue to medical utilization, occupational accident and sickness absence. Results: In logistic regression analyses, self-perceived fatigue was associated with medical utilization (outpatient), occupational accident and sickness absence although the strength of the associations was higher in the blue collar workers except for medical utilization. For medical utilization, the white collar worker's adjusted odds ratios of outpatient utilization for workers scoring in the third quartile and the highest quartile, compared to workers scoring in the lowest quartile were 1.50 (95% CI: 1.20-1.87) and 2.16 (95% CI: 1.69-2.75), respectively. The blue collar workers' adjusted odds ratios of outpatient utilization for workers scoring in the second (OR: 1.23, 95% CI: 1.06-1.43), third (OR: 1.42, 95% CI: 1.22-1.66) and highest quartile (OR:1.83, 95% CI: 1.55-2.17), respectively, compared to workers scoring in the lowest quartile were statistically significant. However, no associations were found between fatigue and inpatient utilization for either the white or blue collar workers. The blue collar workers' adjusted odds ratios of being injured for workers scoring in the second, third and highest quartile were 1.41 (95% CI: 1.00-2.00), 1.57 (95% CI: 1.10-2.22) and 2.41 (95% CI: 1.69-3.44), respectively, compared to those scoring in the lowest quartile, but, those of the white collar workers were not significant. There was more likely to an increased risk of sickness absence in workers scoring in the second, third and highest quartile compared to those scoring in the lowest quartile in both white and blue collar workers, but the magnitudes of risk were higher in the blue collar workers than the white collars workers. Conclusion: This result suggests that fatigue is a determinant predictor of medical utilization, occupational accident and sickness absence, and that the pattern of risks for the three outcomes differs according to the occupational type. Some limitations of this study and considerations for future study were also discussed.

과학 수행 평가 문항의 선정 및 제작을 위한 평가 준거의 개발

김은진,박현주,강호감,노석구 한국과학교육학회 2003 한국과학교육학회지 Vol.23 No.1

본 연구에서는 과학교육에서 수행평가의 올바른 사용과 정착을 위하여 과학 수행평가문항의 선정과 제작을 위한 평가준거를 개발하였다. 이를 위하여 과학과 평가목표 및 수행평가에 대한 이론적 논의를 통해 평가준거의 대범주로 평가 목표의 충실도(Fidelity), 수행평가의 특징과 구성 요소에 대한 만족도(Satisfaction), 문항의 내용 타당도(Content Validity), 학습자에 대한 내용의 적합도 및 공정성(Suitability & Fairness), 문항의 신뢰도와 객관도(Reliability & Objectivity), 사용상의 편의성 및 실용성(Usability)의 6가지 범주를 개발하였고, 각 대범주별 하위항목을 선정하여, 총 25개의 하위 항목을 가진 평가준거표를 개발하였다. 그리고 개발된 평가준거표의 타당도는 과학교육 연구자 5명의 안면타당도로 보았고, 신뢰도는 3가지 종류의 과학 수행평가문항을 과학교육 전문가 2명과 초등예비교사 6명이 평가하교, 그 결과를 Kendall계수를 통한 평가자간 합치도로 검증하였다. 그 결과 합치도는 통계적으로 유의한 수준으로 나타났다. 이상의 과정을 통하여 최종적인 평가준거표를 완성하여 제시하였다. We have developed of a criterion that would help to select and develop performance assessments in science education. There are six categories of a criterion: Fidelity, satisfaction, content validity, fairness & suitability, reliability & objectivity, and usability. There are the total of 25 sub-categories under the six categories. Five science educators evaluated the validity of the criterion. For reliability of the criterion, Kendall's tau-b was used. Eight science educators and elementary teachers evaluated three performance assessment tasks for the correspondence of the criterion. This study also discuss the implications of this criterions as well.

녹동균 세포외막 단백질 백신 CFC-1-101의 안정성 및 면역원성 검토 : 임상 제 Ⅰ/Ⅱa상 시험

장인진,김익상,유경상,임동석,김형기,신상구,장우현,박완제,이나경,정상보,안동호,조양제,안보영,이윤하,김영지,남성우,김현수 대한감염학회 1998 감염 Vol.30 No.3

목적 : 제일제당에서는 녹농균의 세포외막 단밸질을 유효성분으로 하는 백신인 CFC-101을 개발하였으며, 동물시험에서 이 백신의 안전성과 유효성을 입증하였다. 본 연구에서는 이 녹농균 백신의 인체에 대한 안전성과 면역원성을 평가하는 동시에 인체 접종시의 최적 투여 용량을 결정하기 위하여 제 I/Ⅱa상 임상시험을 수행하였다. 방법 : 건강한 성인 남자를 피험자로 선별하여 각 용량군에 백신투여자 6명, 위약투여자 2명을 배정하였다. 백신 투여군은 0.25mg, 0.5mg 또는 1.0mg 용량의 녹농균 백신을 7일 간격으로 3회에 걸쳐 근육주사 하였으며, 위약 투여군에게는 세포외막 단백질을 제외한 동일한 성분을 투여하였다. 백신접종 후 국소적 또는 전신적인 반응의 발생여부를 관찰하고, 혈액시료를 체취하여 백신의 역가와 유효성을 검정하였다. 결과 : 녹농균 백신 CFC-101은 모든 접종자에서 양호한 내약성을 보였다. 또한 0.5mg 과 1.0mg 백신 투여군에서는 100%의 항체양전율을 나타내었다. 생성된 항체는 녹농균 세포외막단백질에 특이성을 보였고, 녹농균 감염에 대해 방어효능이 있었다. 결론 : 이와같은 결과로부터 이 녹농균 백신은 인체에 안전하게 투여할 수 있으며, 높은 항체 생성능으로 감염방어 효능을 보이고 0.5mg과 1.0mg이 최적용량인 것으로 판단되었다. Background : We developed a Pseudomonas aeruginosa outer membrane protein(OMP) vaccine CFC-101, and the prophylactic efficacy of which has been demonstrated in animal models. In order to evaluate the safety and immunogenicity of the P. aeruginosa vaccine, we carried out a phase I/Ⅱa clinical trial in healthy male volunteers. Methods : Groups of eight volunteers, including two placebo subjects, were vaccinated intramuscularly with three doses of 0.25, 0.5 or 1.0 mg of the vaccine at one week intervals. Sings of systemic and local reactions observed after vaccination were recorded for each vaccinee for 5 days. Physical examinations were performed on days 0, 1, 7, 8, 14, 15, 21, and 42, and clinical laboratory tests were done on days 0, 3, and 21. Blood samples for assay of serum antibody levels were obtained up to 42 days after the first vaccination. Results : The vaccine was generally well tolerated by all vaccinees, showing no significant side effects. In the three dosage groups, all vaccinees, except one receiving the 0.25 mg dose, showed significant elevation in serum IgG antibody titers against the vaccine proteins, indicating 100% seroconversion in 0.5 and 1.0 mg groups. The human antibodies induced by the vaccine were specific for P. aeruginosa OMPs, as confirmed by western blot analysis and immunoprecipitation assays. The capacity of the human antisera to enhance opsonophagocytic killing activity by polymorphonuclear leukocytes and to confer protection against P. aeruginosa infections indicates that the antibodies elicited by the vaccine have protective efficacy. Conclusion : We conclude that the P. aeruginosa OMP vaccine is safe and effective for human use and its optimal dose to be 0.5 or 1.0 mg.

연령증가에 따른 혈압요소들의 좌심실비대 및 관동맥질환과의 관련성

박재석,박창규,박미영,박재형,김용현,나진오,신성희,서순용,홍순준,박성미,임홍의,김응주,서홍석,오동주,노영무 대한순환기학회 2004 Korean Circulation Journal Vol.34 No.2

축산분뇨에서 분리한 세균의 동정 및 효소학적 특성

김진선,정소선,이준석,최미영,서승염,이현환 공주대학교 자원재활용 신소재 연구센터 2002 센터사업 성과집 Vol.- No.1

축산 분뇨의 퇴비화에 관련되는 세균들을 분리하고 이들이 생산하는 효소들 중 퇴비화에 관련되는 것으로 사료되는 amylase, cellulase, protease 및 lipase의 특성을 연구하였다. 발효 중에 있는 축산 분뇨로부터 24주의 균주를 분리하여 이중 protease, cellulase, amylase, 그리고 lipase의 활성을 모두 가진 6개의 균주들을 선별하였다. 분리한 6개의 균주들을 동정해본 결과 Corynebacterium varibilis, Bacillus spp., Pseudomonas spinosa, Acetobacter calcoaceticus 및 Athrobacter cumminsii 등으로 밝혀졌다. 이들이 분비하는 효소들의 특징은 중성에서 알칼리성에 이르는 광범위한 pH에서 효소들이 높은 활성을 보였으며, cellulase를 제외한 대부분의 효소들의 최대 활성 온도가 60℃ 정도였으나 cellulase의 경우 37℃가 최적 활성 온도였다. 이는 발효가 진행되어 축산 분뇨에 고온의 열이 발생할 때, 이 환경 하에서 유기물을 분해함으로써 발효과정을 원활히 진행시키는 것으로 생각된다. 다만 cellulase 생산 세균의 경우 축산 분뇨의 초기 발효시에 관여하여 균주의 성장 및 유기물의 분해에 관여하는 것으로 사료된다. 이와같은 결과는 축산분뇨 발효 초기와 발효후기의 온도가 상승된 환경에서 작용하는 세균의 균총이 다름을 암시하고 있다. To develop the effective composting system, we isolated bacteria that have the abilities to degrade organic matters such as cellulose, carbohydrate, protein and lipid during the compositing of livestock manure. Among 24 strains, 6 bacteria have all the enzymatic activities of protease, amylase, cellulase and lipase. These microorganisms were identified as Corynebacterium varibilis, Bacillus spp., Pseudomonas spinosa, Acetobacter calcoaceticus and Athrobacter cumminsii. All the enzymes produced by the bacteria showed activities at the broad pH range and the maximal activities were obtained at 60℃. It seemed that after the increase of temperature caused by fermentation of livestock manure, the enzymes started to degrade the raw materials, which are added for the control of humidity. However cellulase activity was maximum at 37℃, suggesting that the cellulase-producing bacteria work at an early stage of livestock manure fermentation to provide the organic material for the growth of other bacteria. The production of the enzymes were growth-associated and maximal activities appeared at the early stationary phase of growth.