矮小條蟲(Hymenolepis nana)에 대한 Albendazole의 영향에 관한 電子顯微鏡的 硏究

王辰聲,金洙鎭,林漢鍾 고려대학교 의과대학 1987 고려대 의대 잡지 Vol.24 No.3

Newly developed albendazole (Zentel®) is a broad spectrum anthelmintic with high activity against intestinal nematodes as well as trematodes and cestodes infections. The present study was undertaken to observe the morphological changes of Hymenolepis nana which obtained from experimentally infected mice after treatment with albendazole. For this study 20 mice were artificially infected with eggs and 16 of them were given albendazole (1×100mg/kg) four weeks after infection. The worms were collected from the small intestine of the mice which were autopsied at 1 and 2 hours after treatment. The fine structure of the collected worms were studied by means of scanning and transmission electron microscopies. The findings were compared with those of untreated worms. The results are as follows: 1. The worms obtained from non-treated mice showed that the whole surface is covered with many microtriches. Microtriches of H. nana may be divided into three anatomical regions; a base, junctional region and cap. Tegumental layer showed well developed syncytium which included a lot of number of discoidal secretory bodies. 2. Many blebs were formed on the surface of the damaged tegument of the worm at 1 hour after treatment with albendazole. The bleb was packed by plasma membrance and included matrix which uniform electron density. Some bleb surface has microtriches. 3. The destruction of tegumental syncytium was recognized at 2 hours after treatment with albendazole. Therefore the blebs on the worm sufrace caused to the death by the tegumatal bursing.

디지털 카메라를 이용한 필름 두부방사선사진의 디지털 이미지 전환

왕성진,김경호,최광철 대한치과교정학회 2004 대한치과교정학회지 Vol.34 No.5

환자의 진단과 치료계획 수립시 필수적인 두부방사선사진 분석을 위한 컴퓨터 프로그램의 개발로 기존의 필름두부방사선사진을 디지털 이미지로 전환시키는 과정이 필요하게 되었다. 이 때 디지털 카메라를 이용할 경우 초점거리에 따른 이미지의 왜곡이 발생하는데 이는 두부방사선사진 분석시 계측치의 오차를 발생시킬 수 있다. 또한 이미지 크기와 압축비에 따른 디지털 이미지의 질에 의해서도 두부방사선사진 분석시 가장 중요한 과정인 계측점식별에 있어 오차를 가져올 수 있다. 따라서 본 연구에서는 COOLPIX4500 디지털 카메라(Nikon, Japan)를 이용하여 기존의 필름 두부방사선사진을 디지털 이미지로 전환시 최소한의 왜곡을 보이는 초점거리를 결정하고 필름 두부방사선사진상에서 보이는 수준의 정확한 계측점 식별을 위한 최소한의 디지털 카메라 셋팅에 관하여 조사한 결과 다음과 같은 결론을 얻었다. 기존의 필름 두부방사선사진 촬영시 초점거리 16.4mm (35mm 필름 카메라로 환산시 79.4mm)에서 최소한의 상의 왜곡을 보였다. 초점거리가 16.4mm보다 짧은 광각(wide) 촬영시 상의 원통형 왜곡(barrel distortion)이 발생했고 16.4mm 보다 긴 망원(tele) 촬영시 상의 실패형 왜곡(pincushion distortion)이 발생했다. 필름 두부방사선사진상에서 직접 손으로 tracing한 필름 이미지 셋팅 수준의 정확한 계측점 식별을 위한 최소한의 디지털 카메라 셋팅은 2272×1704 pixel normal(1/8) compression 이었다. 따라서 COOLPIX4500 디지털 카메라(Nikon, Japan)를 이용하여 기존의 필름 두부방사선사진을 디지털 이미지로 전환하는 경우 초점거리 16.4mm에서 최소한 2272×1704픽셀(pixel) 이미지 사이즈와 normal (1/8) 압축비의 셋팅을 설정하여야 한다. As computer programs for cephalometric analysis were developed in diagnosis & treatment planning, digital imaging of film-based cephalograms came to be needed. When a digital camera is used, a problem encountered is the image distortion produced according to the focal length, which causes errors in indentifying landmarks. In addition, changes in the image size and compression ratio will inevitably produce a low quality image, causing errors in identifying landmarks. Hence, we have found the focal length producing the least image distortion when digital imaging the film-based cephalograms and the minimal digital camera setting which helps to identify the correct landmarks using the COOLPIX4500 digital camera (Nikon, Japan). The results were as follows: The image distortion was minimized at a focal length of 16.4mm (79.4mm when converted into a 35mm film camera) when digital imaging the film-based cephalograms. When wide imaging, with a focal length of under 16.4mm, barrel distortion was found and when tele imaging, with a focal length of over 16.4mm, pincushion distortion was found. The minimal digital camera setting was 2272×1704 pixel at normal (1/8) compression from which we can identify the correct landmarks at the same level as tracing the film-based cephalograms manually. As a result, when digital imaging the film-based cephalograms, using a COOLPIX4500 digital camera (Nikon, Japan), the focal length should be 16.4mm, the pixel image size over 2272×1704, and the compression ratio over normal (1/8).

유산균(L.lactis CBT-8)및 발효 추출물의 Helicobacter pylori 억제 능력

진춘조,박형석,이혜운,김성렬,강동구,이준상,이재동,왕준호 건국대학교 의과학연구소 2002 건국의과학학술지 Vol.12 No.-

Helicobacter pylori infection was the cause of chronic gastritis, duodenal ulcer and gastric MALT lymphoma. The eradication rates of H. pylori using antibiotics are around 80%. Lactobacilli have been demonstrated to have in vivo and in vitro inhibitory effects on H. pylori infection. We aimed to evaluate the inhibitory effect of L. lactis CBT-8 and its extract on H. pylori in human stomach. Thirty-five H. pylori-infected volunteers(30-49 yr)were randomized into two groups which were treated with L. lactis CBT-8 and its extract(Group A n=17) or milk containing L. lactis CBT-8(Group B, n=18). They underwent 13C-urea breath tests before and after treatment. The DOB30 of group A(n=14) was decreased 41.0% after treatment(26.0±4.9 before, 15.3±2.1 after treatment)(p<0.05). the DOB30 of group B(n=13) was decreased 22.3% after treatment(22.0±2.2 before, 17.1±1.7 after treatment)(p<0.05). In conclusion, L. lactis CBT-8 was effective in suppressing H. pylori infection in human stomach.

Polylysine을 입힌 배양면이 GH₃ 뇌하수체 종양 세포에 미치는 영향에 관한 연구

김정진,백상호,황덕호,황영일,이왕재,이병란 대한해부학회 1998 Anatomy & Cell Biology Vol.31 No.3

Biuret 킬레이트 산소배위 크롬(III)착물의 전자전이선 분할 경향 및 리간드장 분석

박성진,이규왕 명지대학교 자연과학연구소 1998 자연과학논문집 Vol.17 No.-

Urea의 chelate형태인 biuret이 Cr(III)전이금속에 tris로 배위된 Cr(biuret)??을 합성하고, 이의 분광학적인 특성을 조사하였다. 저온 (12K)에서의 발광측정으로 100μsec의 비교적 긴 lifetime을 가지는 인광이 검출되었으며, 유사 착물에서 관찰되었던 형광은 관찰되지 않았다. 발광스펙트럼에서 나타나는 진동피크들과 IR과 Raman 진동 data의 비교분석으로 육배위된 산소와 크롬(III)금속간의 여러 진동모드들을 결정하고 이를 바탕으로 저온의 여기스펙트럼에서 관찰되는 피크들을 분석하였다. 연구결과,d-d 전이에서 금지된 배치내 전이인 doublet² Eg및² T??전자전이선들이 결정되었고 비교적 강한 donor로 관찰되었다. 자세한 전자전이선의 분할 경향 및 이에 따른 발광특성, 배위내 geometry그리고 비등방성 π결합에 의한 효과 등이 본문에서 논의될것이다. Chromium(Ⅲ) complex with biuret, a chelate form of urea, was synthesized and its spectroscopic behaviour was observed. Only phosphorescence of 100 μsec lifetimeshould be found at low temperature and there was no evidence for fluorescence emission in any conditions, even though it was found at similar hexaurea chromium complex. Cr-O based strectching and bending vibrational modes in luminesecnce specturm were assigned by comparison with IR and Raman spectra. These peaks make it possible to determine five lines of spin forbidden doublet electronic transition, ⁴A₂? ↔²E?, ²T₁?(O?). Observed splittings were reproduced from AOM (Angular Overlap Model) based ligand field calculations. Detailed analysis regarding electronic transition behaviour, coordination geometry and anisotropic bonding will be discussed in the text.

난소절제후 Estrogen을 투여한 흰쥐 대동맥에서 Protein Kinase C-α와 Fas 발현의 변화

이왕수,이상엽,안지현,송영빈,김학진,이광재,유재격,김상욱,김태호,김치정,류왕성 중앙대학교 의과대학 의과학연구소 2001 中央醫大誌 Vol.26 No.3

Estrogen is believed to decrease coronary artery disease. Protein kinase C-α(PKCα)appear to be important in signal conduction pathways. Estrogen treatment increases catalytic activity of PKCα,and activation of PKCαcan modulate estrogen receptor levels and responsiveness. And it was reported that activation of PKC can protect cells from apoptosis induced by Fas ligation. But the beneficial effect of estrogen on PKCαwas not clearly demonstrated. The objective of this study was to evaluate the role of PKCαafter ovariectomy and estrogen therapy in rat aorta. The results were summarized as below: 1. The significant pathological changes were not observed in the rat aorta irrelevant to ovariectomy and estrogen therapy. 2. The expression of Fas was decreased in the aorta of estrogen-treated rats than that of ovariectomized rats without estrogen therapy. 3. Increased expression of PKCαwas more marked in the aprta of estrogen-treated rats than that of ovariectomized rat without estrogen therapy. In conclusion,PKCαmay be important in signal conduation pathway on the effect of estrogen. Activation of PKCαby estrogen reduced Fas expression, suggesting that PKCαactivation may play a role in protection against atherasclerosis. Further studies are needed to elucidate the role of PKCαafter estrogen therapy.

Sodium Trans-Bis(methyliminodiacetato)Chromate(Ⅲ)의 두가지 다른 空間群 P1￣와 A2/n間의 相互 變換

李珍昊,宋柱昊,崔成山,郭相姬,吳美蘭,박성진,李揆旺,徐廷善,徐日煥 충남대학교 1996 忠南科學硏究誌 Vol.23 No.1

The structure of sodium trans-bis(methyliminodiacetato) chromate(Ⅲ), C_10H_14O_8N_2CrNa, has been elucidated with two space groups P 1-; a=8.9856(6), b=13.308(1), c=6.261(1)Å, α=76.38(1), β=110.361(9), r=107.394(6)˚, V=662(2)Å^3, Z=2, μ=0.47mn^(-1), d=1.831Mg/㎥, R=0.0295 for 1901 independent reflections with F^2>2σ(F^2), and A2/n; a=13.65(5), b=6.261(1), c=16.85(6)Å, β=113.05(1)˚, V=1324(7)Å^3, Z=4, R=0.0287 for 1013 independent reflections with F^2>2σ(F^2). There are two different asymmetric half molecules in triclinic system and one asymmetric half molecule in monoclinic system. The structures of three different molecules are very similar in conformations. The four carboxyl O atoms and the two imino N atoms in each molecule produce a distorted octahedral coordination geometry around the Cr atom. The average distances of Cr-O(carboxyl) and Cr-N(imino) are 1.956 and 2.061Å respectively. Molecules are held together by ionic forces. In this paper, it has been also shown that the cell constants, Miller indices, atomic coordinates, lattice directions and reciprocal lattices of the two space groups can be mutually transformed.

Campylobacter pylori 위장내 분리위치에 따른 병원성

한왕수,한상진,김정목,조양자 대한감염학회 1989 감염 Vol.21 No.1

Compylobacter pylori may cause gastritis and has been proposed as an etiologic factor in the development of peptic ulcer. However, it is unknown where is primary colonization sites of C. pylori. Isolation sites of C. pylori in stomach and duodenum and pathology in antrum were evaluated in 90 subjects endoscoped at Hanyang University Hospital in Seoul, Korea. C. pylori detected in biopsied specimens of 40 (74%) of 54 C. pylori-positive patients at antrum only, and 13 (24%) of the same patients at antrum and body, but did not detected in duodenal biopsied specimens. Antral pathology results were chiefly found in active chronic gastritis. These findings strongly suggest that antrum is a primary colonization sites of C. pylori and the agent may be spread within stomach from the antrum.

Low doses of ionizing radiation suppress doxorubicin-induced senescence-like phenotypes by activation of ERK1/2 and suppression of p38 kinase in MCF7 human breast cancer cells

신재식,우상혁,이형찬,홍승우,유두현,홍석일,이왕재,이명석,진영우,안성관,진동훈,박인철 Sookmyung Women's University Research Institute of 2010 여성과 건강 Vol.6 No.1

정신분열병에 대한 Risperidone의 효과 및 안전성에 관하여

신석철,왕성근,지익성,이선우,이규광,이봉희,이진영,황선희,신용재,배경도,김정란 충남대학교 의과대학 지역사회의학연구소 1997 충남의대잡지 Vol.24 No.1

In order to evaluate the efficacy and safety of risperidone, 10 patients with chronic schizophrenia were examined for 8 weeks in a silgle-open study. After the wash-out period of 1 week, risperidone was administered. Efficacy was evaluated by means of Positive and Negative syndrome Scale for Schizophernia(PANSS) and Clinical Global Impression(CGI). Safety assessment included the Extrapyramidal Symptom Rating Scale(ESRS), vital signs, body weight, ECG, and laboratory tests. The results were as follows 1) On the PANSS total score, there was significant improvement of total score after 1 week (P<.05) of administratiom. 2) On the PANSS positive and negative subscal, there were significant improvement of posotive and negative scores after 2 weeks (P<.05) of administration. 3) On the PANSS general psychopatholgy subscals, there were significant improvement of general psychopathologyscores after 1 week (P<.05) of administratiom. 4) On the CGI, there was significant decreasement of clinical impression of severity of schizophrenia score after 2 week (P<.05) of administration. 5) A statistically significant increase in body weight (P<.05) was observed after 8 weeks of administration. 6) EPS reached the peak at the end of the 1st week of administration of risperidone (11.8± 24.25) but they were easily relieved by addition of benztropine and clonazepam. There was not significant change in laboratory tests, vital sign, ECG after 8 weeks of administration. These results suggest that Risperidone is an effective antipsychotics and clinically safe except for increased body weight in chronic schizophrenia.