Production and Characterization of Enzymes Involved in Chitin Catabolic Cascade from a Bacterial Strain Isolated from Soil

( Ji Hyun Lee ),( Jeong In Lee ),( So Yeon Park ),( Yoo Kyeong Jang ),( You Bin Lim ),( Hye Yoon Lee ),( Young Min Jung ),( Hae Chang Lee ),( Jong Hwa Lee ),( Shaheen Amna ),( Jae Kweon Park ) 한국키틴키토산학회 2019 한국키틴키토산학회지 Vol.24 No.1

The present study was aimed to isolate the bacteria producing chitinolytic enzymes including chitin deacetylase (CDA) from soil origin. About 24 bacteria were screened for their chitinolytic enzymes producing ability on the basis of colloidal chitin. Amongst one of the most potent isolates designated to JI-02 was further selected as the most prominent chitinolytic and CDAlike enzyme (CDA-X) producer based on the ability of deacetylation of an artificial substrate pNP-acetamilide. The maximum production of CDA-X in crude enzyme from JI-02 was observed in the presence of 1% colloidal chitin at 37°C and pH 6.4 after 120 h of incubation. Potent enzyme activity of CDA-X was determined toward pNP-acetamilide, demonstrating that optimal pH, temperature and ionic strength of the CDA-X were assessed to be 7.0, 52°C and 125 mM, respectively. Unexpectedly, Nacetylglucosamine (GlcNAc) was not fully converted by the action of CDA-X to glucosamine (GlcN) under the established conditions. Further study on enzyme activity toward chitin-oligosaccharides consisting of multi-N-acetylglucosamine (GlcNAc)n, n=2-5 may be necessary to elucidate the mode of action which requires the minimum size of (GlcNAc)n. However, our data suggest that CDA-X can convert chitin to chitosan with the maximum yield of approximately 0.08g/L during the fermentation of the strain in the presence of chitin. Furthermore, we found that crude enzyme showed the significant chitosanolytic activity. Taken together, our data suggest that the strain JI-02 may be a potential candidate as a particular strain for better understating of chitin catabolic pathway.

여성농업인 노동의 경제적 가치평강 관한 연구 : 농업노동과 가사노동 Agricultural Work and Household Work

유소이,최윤지,조현숙,김경미 대한가정학회 2003 Human Ecology Research(HER) Vol.41 No.7

The purposes of this study were to explore the rural women's labor by measuring labor value economically. To achieve the purpose, this study applied four methods : replacement cost method individual function, replacement cost method generalist, opportunity cost method and shadow wage method. The results of this study were as follows; 1) Time used for agricultural work and household work by rural women were 5.3 hours and 3.8 hours each. 2) According to the methods used, the amounts of valuing rural women's labor were varied and ranged from 23,000 won to 43,000 won per day. This study might help recognize the degree of rural women's labor contribution to the household income of farm households and improve the socio-economic status of rural women through showing the productivity of the rural women's labor.

소배양법으로 배양한 생쥐췌장 베타세포의 형태학적 검정

유광성,전현우,박주홍,윤지원,고광삼 朝鮮大學校 附設 醫學硏究所 1986 The Medical Journal of Chosun University Vol.11 No.1

생쥐췌장 베타세포를 보다 순수하게 배양하기 위해 변형 고안한 소배양법을 검정하였다. 이 소배양법에 있어서는 96well plate에 islet cell을 이식하고 decantation한 다음 thimerosal에 노출시켜 많은 viable cell을 얻을 수 있었고, 생후 7일째의 생쥐의 췌장베타세포의 생존율이 가장 높았다. 또 이 소배양법으로 배양한 생쥐췌장 베타세포들은 위상차현미경, FITC-labelled anti-insulin antibody 염색 및 전자현미경으로 검정한 결과 형태학적으로 정상이었다. 기계적 선택방법으로서 생쥐췌장 베타세포의 decantation 최적시간은 세포를 이식한 후 12∼15시간 이었으며, 화학적 선택방법으로 0.6∼0.8㎍/㎖의 thimerosal에 노출시켜 fibroblastoid cell로 부터 생쥐췌장 베타세포의 분리를 증가시킬 수 있었으며, 두가지 방법을 동시에 응용하여 더 좋은 효율을 얻을 수 있었다. 또 이 소배양법에 있어서 cell duplication은 배양후 36시간후에 2배, 72시간후에 3배가 되었으며, 평균 generation시간은 46.8±7.0시간 이었다. 이들 결과로 미루어 보아 이소배양법은 생쥐췌장 베타세포 배양의 유용한 방법으로서, 췌장 베타세포에 연관된 각종 연구에 이용할 수 있을것으로 사료된다.

요로결석 환자에 대한 응급실에서의 진단방법

유지영,최옥경,유진현,정구영 大韓應急醫學會 1997 대한응급의학회지 Vol.8 No.1

Nephrolithiasis is one of the most common disease seen in the Emergency Department. Among the patients who visited in the Emergency Department with acute abdominal pain, many people were suspected urolithiasis. Because of limitation of diagnostic method on Emergency Department we planned this study in order to confirm the our accuracy of diagnosis and the possibility of misdiagnosis. During 5 months from April 1996 to August 1996, the 189 patients was suspected the urolithiasis. Among this patient, 51 cases was excluded due to incomplete study. After performing P/Ex, U/A, KUB in the Emergency Department, they were followed out patients department of Urology and perfomed Intravenous Pyelogram. The sensitivity and specificity for KUB were 84.5% and 94.0%, respectively. The positive predictive value was 93.8%, the nagative predictive value was 93.8%. Diagnostic accuracy of urolithiasis in our Emergency Department is 89.1%. There was no relationship between urinary occult blood and KUB or IVP. There was no relationship between degree of occult blood and diagnosis of urolithiasis. For differential diagnosis of urolithisis, emergency ultrasound and IVP will be available. So combination of this diagnostic methods will improve accuracy and safty of diagnosis and treatment of urolithiasis.

우리나라 유방아마에서 CYPIA1, EPHX 유전자 다형성의 역할

최지엽,유정희,이은옥,유근영,박수경,안세현,노동영,최국진,강대희 대한암예방학회 2002 Journal of cancer prevention Vol.7 No.3

석면관련 폐질환의 진단에 있어서 흉부고해상도전산화단층촬영검사(HRCT)의 유용성

이현재,손지언,,홍영습,,이영일,예병진,유창훈,정갑열,김상훈,임형준,장은철,김정만,김준연 大韓産業醫學會 2006 대한직업환경의학회지 Vol.18 No.2

목적: 석면에 노출된 근로자에서 석면관련 폐질환의 양상을 분석하고 진단에 있어 HRCT의 유용성을 연구하여 석면에 대한 의학적 감시프로그램의 개발에 기여하고자 하였다. 방법: 석면에 직접 노출되는 작업자 68명과 간접 노출되는 작업자 94명 등 총 162명에 대해 OSHA 석면 표준, 의학적 감시 프로그램에 따라 단순 흉부방사선검사, 폐활량검사 및 우리나라 실정에 맞게 일부 수정한 의학적 설문지를 통해 면접조사의 방식으로 환자에 관한 정보를 수집하였으며, 자료의 신뢰성을 확보하기 위하여 면접조사는 1달 이상의 기간을 두고 2차례에 걸쳐 실시된 후 확인, 수정하였다. 또한, 석면관련 폐질환을 확진하기 위하여 HRCT를 추가적으로 실시하였다. 결과: HRCT결과 석면관련 폐질환의 유병율은 162명의 근로자중 17명으로 10.5%였는데, 직접 노출군에서 10명 (14.7%), 간접 노출군에서 7명 (7.4%)이었다. HRCT와 비교할 때 단순 흉부방사선검사의 민감도와 특이도는 각각 70.6%와 98.6%이었고, 양성 예측도와 음성 예측도는 각각 85.7%와 96.6%으로, HRCT는 단순흉부방사선검사에 비해 민감도가 높아 초기 석면관련 폐질환을 진단하는데 효과적이었다. 석면관련 폐질환의 진단에 있어 HRCT와 폐활량검사 사이에 유의한 관련성이 관찰되었다. HRCT 결과 석면관련 폐질환의 유무와 의학적 설문조사 결과의 빈도분석을 실시한 결과, 유의한 변수는 작업기간, 흡연량, 호흡기질환의 과거력, 기침, 호흡곤란이었다. 결론: HRCT는 석면관련 폐질환의 진단에 있어 단순 흉부방사선검사와 비교하여 민감도가 높아 초기 진단에 유용하므로 단순 흉부방사선선검사 결과 석면관련 폐질환소견을 보일 때뿐만 아니라, 정상소견을 보이더라도 폐활량 검사결과와 근무력, 흡연력, 호흡기질환의 과거력, 기침, 호흡곤란 등의 호흡기 증상을 참고하여 HRCT검사를 고려해야 한다. 또한, 본 연구에서 관련성이 없었던 요인들에 대해서도 향후 연관성을 규명하는 광범위한 전향적 연구가 필요하다. Objectives: This study was carried out to improve the medical surveillance program of workers exposed to asbestos by examining the usefulness of High Resolution Computed Tomography (HRCT) in the diagnosis of asbestos-related lung disease. Methods: The study subjects comprised 162 workers in a ship-repairing yard, 68 of whom had been directly exposed to asbestos and 94 indirectly exposed. The 'Occupational Safety & Health Administration (OSHA) asbestos standard, medical surveilance program' and HRCT were conducted to analyze the aspects of the asbestos-related lung disease. The OSHA asbestos standard, medical surveilance program consists of simple chest x-ray, spirometry and medical questionnarie. Results: Seventeen (10.5%) of the 162 subjects, 10 (14.7%) directly exposed and 7 (7.4%) indirectly exposed, revealed asbestos-related lung disease on HRCT. The sensitivity and specificity of simple chest x-ray for asbestos-related lung disease were 70.6% and 98.6%, and the positive and negative predictive values were 85.7% and 96.6% respectively, as compared with HRCT, HRCT was an effective diagnostic tool especially to detect early asbestos-related lung disease. The study results indicated a relative significant association between the results of spirometry and HRCT. The variables significantly associated with asbestos-related lung disease on HRCT were work duration, smoking history (pack-years), past history of respiratory disease, cough and dyspnea. Conclusions: In the diagnosis of asbestos-related lung disease, HRCT should be considered not only for workers with positive findings on simple chest x-ray, but also workers with specific findings on spirometry, occupational history, smoking history, and past history of respiratory disease, or with respiratory symptoms such as cough and dyspnea.

기혼여성의 경제활동참가의 변화에 관한 연구

조유현,김지경 중앙대학교 생활과학연구소 2000 생활과학논집 Vol.13 No.-

The purpose of this study was to investigate the factors affecting the changes of the labor force participation rate for married women, and thus, to provide useful information on the related studies and public polices for the labor force participation of married women. Based on the review of literature, labor force participation of married women turned out to be very important issue because of the implications it has on economic, social and even political policies. In order to investigate the change and the trend of the labor force participation for married women, the data conducted by National Statistical Office Republic of Korea was used. And a simple regression was adopted in investigating the factors affecting the change of the labor force participation rate for married women. The labor force participation rate of married women was explained by birth rate, hours of labor per week, the level of education and the rate of urbanization. However, unlike the western countries, the higher education level of married women and the higher rate of urbanization had negative impacts on the labor force participation rate of married women.

비타민 E 농도에 따른 마우스 면역능과 항산화 효소 활성도의 변화

이유숙,이지혜,김현숙 한국노화학회 2001 한국노화학회지 Vol.11 No.1

Chlorpromazine으로 유발된 음경지속발기증 1례

신유호,윤도준,이충현,송지영,이상철 大韓神經精神醫學會 1996 신경정신의학 Vol.35 No.3

저자들은 항정신병약물 chlorpromazine 치료 중에 음경지속발기증이 발생하여 수술까지 시행하여 회복된 환자 1례를 체험하였기에 문헌고찰과 함께 보고하였다. 항우울제 trazodone과 함께 chlorpromazine, thioridazine과 같은 항정신병약물이 음경지속발기증의 원인과 관련이 있는 것으로 알려지고 있다. 이러한 약물에 의해 유발되는 음경지속발기증의 기전에 대해서는 알파-아드레날린 차단으로 인한다는 설이 많은 지지를 받고 있다. 치료는 보존적인 치료와 외과적인 치료가 있으며, 외과적인 치료로는 음경해면체 천자와 흡입 세척술 그리고 분로를 만들어 주는 방법들이 있다. 음경지속발기증은 신속한 진단과 치료를 하지 않을 경우 영구적인 발기불능을 초래할 수도 있는 부작용이므로 향정신약물 투여시 이에 대한 관심과 주의를 가져야 할 것이다. Antipsychotic drugs can induce several sexual side effects. Priapism, one of the side effects, is defined as "the persistent abnormal erection of the penis, which usually occurs without sexual desire". There has been an increasing number of reports recently linking the etiology of priapism with psychotropic medication. The drugs known to be associated with priapism are psychotropic drugs such as trazodone, chlorpromazine, thioridazine as well as antihypertensives, anticoagulants and so on. The mechanism of a drug which induces priapism is proposed to be mediated by its alpha-adrenergic blocking effect. Prompt diagnosis and treatment are essential. Priapism is a severe side effect, which can lead to impotence if prompt diagnosis and treatment are not carried out. There are medical and surgical treatments for priapism. The surgical treatments usually involve aspiration, irrigation and the creation of shunt. We report a case of priapism which developed after two years of chlorpromazine treatment. He is a 20-year-old man with schizophrenia. He experienced two times of brief episode of prolonged penile erection before developing intractable priapism. He was treated by shunt operation which showed satisfactory relief of priapism. It seemed by this case that duration and dosage of chlorpromazine were not closely related with priapism. Psychiatrist is needed to give attention to the priapism as one of sexual side effects due to antipsychotics though it is not common.

심한 척추후측만증 환자의 대퇴골절 복원술을 위한 부위마취 : 증례보고 A case report

김지은,박대용,유시현,강규식,권진형,김천숙,안기량 순천향의학연구소 2004 Journal of Soonchunhyang Medical Science Vol.10 No.2

Kyphoscoliosis is a disease manifested as lateral curvature of spine and accompanying rotation of the vertebrae, resulting in distortion of rib cage. Restrictive lung disease and pulmonary hypertension progressing to cor pulmonale are the major cause of mortality in patients with kyphoscoliosis. We had experienced a successful spinal and caudal epidural anesthesia for the open reduction and internal fixation with plate of fractured femur shaft in two patients with severe kyphosoliosis. We had chosen regional anesthesia as an anesthetic method because of there poor pulmonary function and severe deformities of thoracolumbar spine. We could achieve the adequate anesthetic sensory dermatome level, T_(5) and T_(8), for operation without any significant deterioration of blood pressure and respiration. We recommended that regional anesthesia is a useful anesthetic method for a patients with severe kyphoscoliosis when the operation on hip or lower extremity is required.