심근경색 모델 흰쥐에서 스트레스가 B-type Natriuretic Peptide 발현 및 심박변이율 변화에 미치는 영향

이삼윤(Sam Youn Lee),이미경(Mi Kyoung Lee),김남호(Nam-ho Kim),주민철(Min Cheol Joo),조항정(Hyang Jeong Jo),강지숙(Ji Sook Kang),김병숙(Byung Sook Kim),윤선식(Sun Sik Yoon),최을식(Eul Sig Choi),이문영(Moon Young Lee) 대한스트레스학회 2010 스트레스硏究 Vol.18 No.3

심박변이율의 감소는 심근 경색 환자에서 예후의 악화와 관련되어 있다. 심박변이율 중 very low frequency가 심부전환자의 예후를 판단할 수 있는 독립적 인자로서 사용이 가능하다고 보고된 바 있으며, 심실의 압력 증가에 반응해서 생성되는 B-type natriuretic peptide가 심근 경색의 예후 인자로서 사용할 수 있음 역시 보고된 바 있다. 본 연구에서는 심근경색 모델을 제작하여 일정의 회복 기간을 거친 다음 다시 일정 기간의 스트레스를 겪게 한 후 심박변이율의 변화 및 심실 조직에서의 BNP 발현 정도를 비교하여 스트레스에 의한 영향을 관찰하고자 하였다. Sprague-Dawley계 수컷 흰쥐 15마리를 대상으로 하였다. 실험동물은 인위적 스트레스 및 수술을 받지 않은 대조군(CON, n=5), 심근경색 수술을 받은 후 restraint stress를 받지 않은 군(MI+No Stress, n=5), 심근경색 수술을 받은 후 1주일 동안 restraint stress를 받은 군(MI+Stress, n=5)으로 분류하였다. 심근경색 모델 제작 직후 15분 이상 심전도의 변화를 기록하였으며, 2개월 정도 후 심근경색 수술을 받은 동물을 두 군으로 나누어 그 중 한 군에는 1주일 동안 restraint stress를 가한 후 restraint stress를 가하지 않은 동물과 심박변이율을 비교 분석하였다. 심실 조직은 좌심실 전벽 부위의 위축을 관찰할 수 있었고, 대식세포에 의하여 응고, 괴사된 심근세포의 탐식과 혈관이 풍부한 육아조직 및 섬유 변화를 볼 수 있었다. 심박변이율은 심근경색 수술을 받은 직후 심박수는 유의한 증가를 보였고, standard deviation of the normal to normal intervals (SDNN), very low frequency (VLF) 및 low frequency (LF)의 유의한 감소를 보였다. 심근경색 수술을 받았던 동물에서 두 달 정도의 회복 기간을 거친 후 스트레스를 받지 않은 군은 심박수 및 기타 심박변이율 분석에서 SDNN 값을 제외하고는 정상군과 유의한 차이를 관찰할 수 없었던 반면 1주일 동안 스트레스를 받은 군에서는 심박수가 다시 유의하게 증가했을 뿐만 아니라 SDNN, VLF 및 LF 역시 정상군과 유의한 차이를 보였다. 이상의 결과를 종합하면 심근경색 동물에 대해 1주일 동안 스트레스를 가한 결과 심박변이율이 감소하고 심실에서의 BNP 발현은 더욱 증가하여 악화함을 보여주고 있다. Diminished heart rate variability (HRV) is associated with less favorable prognosis after myocardial infarction (MI). It has been reported that very low frequency (VLF) power in HRV analysis is an independent risk predictor in patients with congestive heart failure and B-type natriuretic peptide (BNP) can be used as a prognostic factor of MI. The purpose of this study was to investigate whether stress has an effect to the changes of BNP expression and/or heart rate variability in MI model in rats. Male Sprague-Dawley rats were divided into 3 groups: control group (CON), MI group (MI+No Stress), and MI followed by stress group (MI+Stress). MI+Stress group rats were raised for a two month recovery period after the operation, followed by being exposed to restraint stress for 2 hours per day for 1 week. Electrocardiogram was recorded after the operation and the last day after 1 week of stress. The frequency components of HRV were calculated in the frequency domain such as VLF, low frequency (LF), high frequency (HF) and so on. In HRV analysis, standard deviation of the normal to normal intervals (SDNN) was significantly reduced in both groups compared to the control group. VLF and LF also were significantly reduced in MI+Stress group compared to the control groups. In addition, BNP expression in western blotting was shown the strongest bands in MI+ Stress group among experimental groups. These results suggest that BNP and HRV were aggravated by stress in MI rat model. (Korean J Str Res 2010;18:275∼285)

국가 음악과 교육과정에 기초한 초·중등학교 음악예술프로그램 적용

석문주,권덕원,최은식,최미영,김명숙,박장미,손미현,정지혜 이화여자대학교 사범대학 교육과학연구소 2011 교육과학연구 Vol.42 No.2

In order to increase opportunities for students to enjoy the musical arts, it is necessary to develop musical arts programs that are based on students’ interests and the national (or provincial) curriculum, that can be systematically and effectively supported by teachers. In this regard, the purpose of this study was to develop those musical arts, carry out the programs in one elementary school and one middle school from October to December 2011, and analyze each implementation. For the analysis, lesson videos, students’ worksheets, in-depth interviews with students, teachers, and teaching artists, and lesson plans were collected. The conclusions and implications are as follows: 1) the role and meaning of musical arts programs based on national curriculum were more highlighted; 2) students could have great depth of aesthetic experience by the means of direct contact with professional musicians and concrete musical experience; 3) the role of music mediators and its importance was magnified; 4) there was a positive change in the musical preferences and attitudes of students with regard to the musical artsprograms. 본 연구는 학생들의 음악 예술 향수기회 확대를 위해 학생들의 흥미와 국가 교육과 정을 중심으로 하는 ‘현장성’, 교사들의 음악 교육을 효과적으로 지원할 수 있는 ‘체계성’, 그리고 음악 예술 전문가들과 학교 사이에 꾸준히 실행될 수 있는 ‘지속성’을 갖춘 음악예술프로그램을 2010년 10월부터 12월까지 초등학교와 중학교에 적용하고 그 결과를 분석하고자 하는데 목적이 있다. 본 연구는 수업 동영상, 학생 활동지, 학생과 교사 심층인터뷰, 강사 인터뷰, 수업안 자료를 수집하여 분석하였다. 본 연구의 결론은 첫째, 국가 교육과정을 반영한 음악 예술프로그램의 역할과 의미가 더욱 확장되었다. 둘째, 음악가들과의 직접적인 만남과 구체적인 예술적 체험을 통하여 학생들은 이전과 비교될 수 없는 ‘깊이 있는 미적 경험’을 하게 되었다. 셋째, 음악메디에이터의 역할이 부각되었다. 넷째, 음악예술프로그램을 통하여 학생들에게 음악에 대한 삶의 변화가 있었다.

골반염에 의한 요관 협착과 신우확장증 1예

김미영,문성택,김윤숙,전섭,최승도,선우재근,배동한 순천향의학연구소 2007 Journal of Soonchunhyang Medical Science Vol.13 No.2

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임신 중기 급성 A형 간염의 치료 1예

김미영,문성택,김윤숙,전섭,최승도,선우재근,배동한 순천향의학연구소 2007 Journal of Soonchunhyang Medical Science Vol.13 No.2

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첨가재료별 증편의 가공 적성 검토

최영희,전화숙,강미영 동아시아식생활학회 1996 동아시아식생활학회지 Vol.6 No.1

The sensory and instrumental characteristics of Jeung-pyun made from various additives were investigated to improve the quality of Jeung pyun and to know the effects of additives on Jeung-pyun preparation. In sensory evaluation of Jeung-pyun made from various additives, hardness was significantly lower by addition of soy bean flour, whole milk powder, and egg yolk. The cell uniformity of Jeung-pyun was significantly lower by addition of egg yolk and mugwort. And the degree of bitterness Jeung-pyun containing soy bean flour and mugwort flour were significantly higher than that of control. Retrogradation of Jeung-pyun assessed from DSC thermogram and hardness measured by texturometer was delayed by addition of soy bean flour, and whole milk powder.

대구지역 대학생의 영양섭취상태, 체형만족도, 체력상태 및 그 관련성

최미자,류숙희,김기진 동아시아식생활학회 1999 동아시아식생활학회지 Vol.9 No.3

The aim of the present study was to evaluate the relations among nutrient intake, self body image and satisfaction, and physical fitness of college students in Taegu. A total of 213 apparently healthy college students living in Taegu were selected for the study. The following anthropometric measurements were made on all participants: weight, height: waist and hip circumferences, and bioimpedence. Using weight and height, the body mass index(kg/㎡) was calculated. The 24-hour recall were obtained from subjects. Dietary habits and energy expenditure were examined through questionnaires. Nutrients intake were analyzed by computer aided nutritional analysis program. Variables of physical fitness were consisted of body fat, muscular strength and endurance, agility, power, flexibility, cardiorespiratory function, physical efficiency index(PEI). Data were analyzed by computer using the SAS 6.12(Statistical Analysis System). The mean heights and weights of these students were similar to those reported for the 50th percentile in a national survey. Nutrient intake of the college students in Taegu was considerably lower than RDA. The male subjects' mean intake of two nutrients, vitamin B₂ and calcium, were less than 55% of the RDAs, the female subjects' mean intake of two nutrients, iron and calcium, were less than 55% of the RDAs. A most variables of physical fitness except sit and reach in male students showed a significant higher values than those of female students. There was a significant positive relationship between PEI and the intake of animal protein, fat, Ca, and B₂ in male students. Also there was a significant positive relationship between exercise duration of PWC 170 and protein, fat, iron, vitamin A, B₂, niacin, and energy intake in female students. The muscular strength showed the significant correlation with weight, height, and waist circumference in male and female students. The cardiorespiratory function and physical efficiency index showed significantly negative correlation with tricep and subscapular skin thickness in the female students. There were significantly positive correlations between WHR and body efficiency index in male students, and significantly positive correlations between WHR and the cardiorespiratory function in female students. There was no significant level of correlation between the degree of satisfaction of body image and physical efficiency index. However, there was a significant positive correlation between waist circumference (and weight) and physical efficiency index in male and female students and almost all of the surveyed male and female college students wanted less waist circumference or weight even though they were in the criteria of standard waist circumference or weight. In conclusion, a beneficial effect of adequate nutrient intake on physical fitness was found, college students must increase their energy and nutrient intake if it is below than the RDA for improving their physical fitness.

성별에 따른 대학생의 내면화된 수치심과 대인관계 문제가 우울증상에 미치는 영향

최재정,김미연,박민지,유미숙 숙명여자대학교 아동연구소 2015 兒童硏究 Vol.27 No.1

본 연구는 대학생의 내면화된 수치심과 대인관계가 우울증상에 미치는 영향력을 규명하고자 한 연구이다. 연구 대상은 서울, 경기 지역의 대학생 404명을 대상으로 자기보고식 설문조사를 실시하였다. 본 연구에 사용된 측정도구는 대학생의 내면화된 수치심, 대인관계, 우울증상의 척도였다. 분석방법은 SPSS 19.0 통계프로그램을 이용하여 빈도와 백분율을 산출하였고, 내적합치도 계수(Cronbach a)를 구하였다. 본 연구문제를 여자 검증하기 위해 중다회귀분석과 단계적 회귀분석을 실시하였고, 밝혀진 결과를 요약하면 다음과 같다. 첫째, 여자 대학생은 사회적 회피와 헌신성이 우울증상과 관계가 있는 것으로 나타났다. 둘째, 남자 대학생은 실수 불안과 지배성, 비주장성이 우울증상에 영향을 미치는 것으로 나타났다. 셋째, 여학생과 남학생의 결과 차이에서 모두 우울 증상에 유의미한 영향을 미치는 것으로 나타났고, 수치심의 하위요인보다 대인관계문제의 하위요인들이 우울 증상에 보다 많은 영향을 미친다. 수치심보다 대인관계 문제가 우울 증상에 보다 큰 영향을 미친다는 결과는 남녀 대학생 모두에게 공통적으로 나타났다.

암환자를 위한 김치의 개발 : 1. 김치레시피조절에 의한 항돌연변이효과 증진연구 1. Studies on Enhancing Antimutagenic Effect of Kimchi by Changing the Recipe

최선미,전영수,이숙희,박건영 부산대학교 김치연구소 2002 김치의 과학과 기술 Vol.8 No.-

To develop kimchi for cancer patient, we studied on enhancing antimutagenic effect of kimchi by manipulating the recipe from the result of prevalence test on kimchi by cancer patients. According to the result of prevalence test, the patients wanted to reduce the level of red pepper powder and increase some sub-ingredients such as garlic and mustard compared to the standardized kimchi. The recipe-manipulated kimchi was fermented at 15℃ for 1 day and then ripened at 5℃ for several days up to pH 4.2~4.3. The antimutagenic effect of the methanol extracts of the kimchi was evaluated by using Ames mutagenicity test with Salmonella typhimurium TA100. Among the kimchi samples, CP_2-M kimchi (2.5% red pepper powder +7.5% mustard leaf added kimchi) exhibited higher inhibitory effect on the mutagenicity induced by aflatoxin B_1 in the Ames test. In vivo supravital staining micronucleus assay, anticlastogenic effect of the CP_2-M kimchi group was higher than that of the control group. The induction of chromosome aberration by mitomycin C was significantly suppressed by CP_2-M kimchi suggesting in post-treatment.

노인의 영적 간호요구

최미혜,김경희,김귀옥,김기숙,김수강,김정신,김춘숙,노흥진,박지연,성혜연,오명선,이선희,이원옥,이윤영,이현수,장명재,차혜경,채정선,홍상희 중앙대학교 의과대학 간호학과 간호과학연구소 2001 중앙간호논문집 Vol.5 No.1

This study was designed to exam the aged's needs for spiritual nursing care. The purpose was to serve as a basis for the development of spiritual nursing practice. The major findings are as follows : 1. The degree of needs for spiritual nursing care as area was that needs of love and relationship mean 22.0, needs of meaning and object mean 28.2, needs of forgiving mean 13.5. Total needs for spiritual nursing care mean 63.7, which was on the upper middle level. The needs of meaning and object was rated highest. 2. Among the general characteristics of the subjects, needs of love and relatiohship wasn't significanlty different. 3. Among the general characteristics of the subjects, needs of meaning and object was significantly different according to two factors : age(F=7.260, p=0.001), religion(F=5.275, p=0.001). Higher needs of meaning and object was possessed by the older than the younger, by the one who have religion than the other. 4. Among the general characteristics of the subjects, four factors made a significantly difference to needs of forgiving : sex(t=-2.851, p=0.006), age(F=8.201, p=0.001), religion(F=6.928, p=0.000), disease(t=2.327, p=0.024). Higher needs of forgiving was possessed by man than woman, by the older than the younger, by the one who have religion than the other, by the one who have disease than the other.

고등학교 여자하키선수에 대한 체력 검사 기준의 타당도

최기수,전혜자,임미숙 순천향대학교 기초과학연구소 2004 순천향자연과학연구 논문집 Vol.10 No.2

The purpose of this study was to confirm the validity of standards in physical fitness test for women high school field hockey players. The results from this study will establish a criterion-referenced standard to distinguish elite players form non-elite players of different performance level. Selected factors of physical fitness were muscular strength, power, agility, flexibility, cardiovascular endurance. And selected physical fitness tests were strength of right hand, strength of left hand, 40m sprint, Illinois agility run, sit and reach, 3,600m run. The result was initially analyzed whit one-way ANOVA to determine if this is any different performance level. The cut-off Scores were determined to establish a criterion-referenced standard of physical fitness test classified by performance level.